Quantitative analysis of immunofluorescence and lipofuscin distribution in human cortical areas by dual-channel confocal laser scanning microscopy

A method for quantitative analysis of confocal laser scanning microscopy (CLSM) images of immunofluorescence and lipofuscin in human brain cortical areas is described. Indirect immunofluorescence was used to show the distribution and density of a synaptic vesicle protein — synaptophysin (p38). Dual...

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Veröffentlicht in:	Journal of neuroscience methods 1996-11, Vol.69 (2), p.155-161
Hauptverfasser:	Belichenko, Pavel V., Fedorov, Alexander A., Dahlström, Annica B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Adult Cerebral Cortex - chemistry Cerebral Cortex - physiology Confocal microscopy Digital image analysis Female Fluorescent Antibody Technique - methods Human brain mapping Humans Image Processing, Computer-Assisted Immunofluorescence Lipofuscin Lipofuscin - analysis Microscopy, Confocal - methods Quantification Software Synaptophysin - analysis
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