High-Efficiency Full-Length cDNA Cloning by Biotinylated CAP Trapper

We have devised a method for efficiently constructing high-content full-length cDNA libraries based on chemical introduction of a biotin group into the diol residue of the cap structure of eukaryotic mRNA, followed by RNase I treatment to select full-length cDNA. The selection occurs by trapping the...

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Veröffentlicht in:	Genomics (San Diego, Calif.) Calif.), 1996-11, Vol.37 (3), p.327-336
Hauptverfasser:	Carninci, Piero, Kvam, Catrine, Kitamura, Akiko, Ohsumi, Tomoya, Okazaki, Yasushi, Itoh, Mitsuteru, Kamiya, Mamoru, Shibata, Kazuhiro, Sasaki, Nobuya, Izawa, Masaki, Muramatsu, Masami, Hayashizaki, Yoshihide, Schneider, Claudio
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Proteins Base Sequence Biological and medical sciences Biotin Brain Chemistry Chromatography, Affinity Cloning, Molecular - methods Diverse techniques DNA, Complementary - genetics Fundamental and applied biological sciences. Psychology Gene Library Glyceraldehyde-3-Phosphate Dehydrogenases - genetics Mice Molecular and cellular biology Molecular Sequence Data Peptide Elongation Factor 1 Peptide Elongation Factors - genetics Ribonuclease, Pancreatic - metabolism RNA Caps - chemistry RNA, Messenger - genetics Streptavidin
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