Ipriflavone does not alter bone apatite crystal structure in adult male rats
We have previously found that a short-term treatment with high doses of ipriflavone increased bone density and improved the biomechanical properties of adult male rat bones, without altering their mineral composition. To determine whether this effect can be associated with alterations of bone crysta...
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Veröffentlicht in: | Calcified tissue international 1996-12, Vol.59 (6), p.496-499 |
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creator | Ghezzo, C Civitelli, R Cadel, S Borelli, G Maiorino, M Bufalino, L Bongrani, S |
description | We have previously found that a short-term treatment with high doses of ipriflavone increased bone density and improved the biomechanical properties of adult male rat bones, without altering their mineral composition. To determine whether this effect can be associated with alterations of bone crystal structure, we have performed X-ray diffraction analysis of bones obtained from rats treated with ipriflavone at doses that were effective in inducing favorable changes on bone density and biomechanics. Eighteen-week-old male Sprague Dawley rats were treated by oral route with either ipriflavone (200 or 400 mg/kg/day), or its vehicle for 12 weeks. The treatment was well tolerated and body weight increased to the same extent in all animals. As a measure of bone crystallinity, we examined the (310) and (002) reflections of the X-ray diffraction patterns, corresponding to the directions perpendicular and parallel to the c-axis of the crystals, respectively. No major differences were observed between ipriflavone-treated and control animals for the broadening parameter beta(1/2) for (310) and (002) peaks, as well as for lattice parameters. Therefore, a 12-week treatment with ipriflavone at high doses does not induce significant modifications of bone "crystallinity." Thus, the positive effect of ipriflavone on bone mineral density appears to be associated with an increased apatite crystal formation rather than an increase of crystal size. These results provide further evidence for the safety and usefulness of ipriflavone in the treatment of osteoporotic syndromes. |
doi_str_mv | 10.1007/BF00369217 |
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To determine whether this effect can be associated with alterations of bone crystal structure, we have performed X-ray diffraction analysis of bones obtained from rats treated with ipriflavone at doses that were effective in inducing favorable changes on bone density and biomechanics. Eighteen-week-old male Sprague Dawley rats were treated by oral route with either ipriflavone (200 or 400 mg/kg/day), or its vehicle for 12 weeks. The treatment was well tolerated and body weight increased to the same extent in all animals. As a measure of bone crystallinity, we examined the (310) and (002) reflections of the X-ray diffraction patterns, corresponding to the directions perpendicular and parallel to the c-axis of the crystals, respectively. No major differences were observed between ipriflavone-treated and control animals for the broadening parameter beta(1/2) for (310) and (002) peaks, as well as for lattice parameters. Therefore, a 12-week treatment with ipriflavone at high doses does not induce significant modifications of bone "crystallinity." Thus, the positive effect of ipriflavone on bone mineral density appears to be associated with an increased apatite crystal formation rather than an increase of crystal size. These results provide further evidence for the safety and usefulness of ipriflavone in the treatment of osteoporotic syndromes.</description><identifier>ISSN: 0171-967X</identifier><identifier>EISSN: 1432-0827</identifier><identifier>DOI: 10.1007/BF00369217</identifier><identifier>PMID: 8939778</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Apatites - chemistry ; Bone Remodeling ; Crystallography, X-Ray ; Femur - drug effects ; Femur - metabolism ; Isoflavones - pharmacology ; Male ; Rats ; Rats, Sprague-Dawley ; Space life sciences</subject><ispartof>Calcified tissue international, 1996-12, Vol.59 (6), p.496-499</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c313t-8dbae2982f1c92ecca9c54227b20c2b35bcc89a5ab53bd60e946ee4d4d6a09ac3</citedby><cites>FETCH-LOGICAL-c313t-8dbae2982f1c92ecca9c54227b20c2b35bcc89a5ab53bd60e946ee4d4d6a09ac3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8939778$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ghezzo, C</creatorcontrib><creatorcontrib>Civitelli, R</creatorcontrib><creatorcontrib>Cadel, S</creatorcontrib><creatorcontrib>Borelli, G</creatorcontrib><creatorcontrib>Maiorino, M</creatorcontrib><creatorcontrib>Bufalino, L</creatorcontrib><creatorcontrib>Bongrani, S</creatorcontrib><title>Ipriflavone does not alter bone apatite crystal structure in adult male rats</title><title>Calcified tissue international</title><addtitle>Calcif Tissue Int</addtitle><description>We have previously found that a short-term treatment with high doses of ipriflavone increased bone density and improved the biomechanical properties of adult male rat bones, without altering their mineral composition. To determine whether this effect can be associated with alterations of bone crystal structure, we have performed X-ray diffraction analysis of bones obtained from rats treated with ipriflavone at doses that were effective in inducing favorable changes on bone density and biomechanics. Eighteen-week-old male Sprague Dawley rats were treated by oral route with either ipriflavone (200 or 400 mg/kg/day), or its vehicle for 12 weeks. The treatment was well tolerated and body weight increased to the same extent in all animals. As a measure of bone crystallinity, we examined the (310) and (002) reflections of the X-ray diffraction patterns, corresponding to the directions perpendicular and parallel to the c-axis of the crystals, respectively. No major differences were observed between ipriflavone-treated and control animals for the broadening parameter beta(1/2) for (310) and (002) peaks, as well as for lattice parameters. Therefore, a 12-week treatment with ipriflavone at high doses does not induce significant modifications of bone "crystallinity." Thus, the positive effect of ipriflavone on bone mineral density appears to be associated with an increased apatite crystal formation rather than an increase of crystal size. These results provide further evidence for the safety and usefulness of ipriflavone in the treatment of osteoporotic syndromes.</description><subject>Animals</subject><subject>Apatites - chemistry</subject><subject>Bone Remodeling</subject><subject>Crystallography, X-Ray</subject><subject>Femur - drug effects</subject><subject>Femur - metabolism</subject><subject>Isoflavones - pharmacology</subject><subject>Male</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Space life sciences</subject><issn>0171-967X</issn><issn>1432-0827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0DtLA0EUhuFBlBijjb0wlYWwOpfduZQavAQCNgp2y5nZs7Aym40zs0L-vQkJWlodODx8xUvIJWe3nDF99_DEmFRWcH1EpryUomBG6GMyZVzzwir9cUrOUvpkjJdKqQmZGCut1mZKlot17NoA38MKaTNgoqshUwgZI3W7H6whdxmpj5uUIdCU4-jzGJF2KwrNGDLtISCNkNM5OWkhJLw43Bl5f3p8m78Uy9fnxfx-WXjJZS5M4wCFNaLl3gr0HqyvSiG0E8wLJyvnvbFQgaukaxRDWyrEsikbBcyClzNyvd9dx-FrxJTrvkseQ4AVDmOqtamUVtr-C3llrdTCbOHNHvo4pBSxrbdZeoibmrN617j-a7zFV4fV0fXY_NJDVPkDMVB3ZQ</recordid><startdate>19961201</startdate><enddate>19961201</enddate><creator>Ghezzo, C</creator><creator>Civitelli, R</creator><creator>Cadel, S</creator><creator>Borelli, G</creator><creator>Maiorino, M</creator><creator>Bufalino, L</creator><creator>Bongrani, S</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>19961201</creationdate><title>Ipriflavone does not alter bone apatite crystal structure in adult male rats</title><author>Ghezzo, C ; Civitelli, R ; Cadel, S ; Borelli, G ; Maiorino, M ; Bufalino, L ; Bongrani, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c313t-8dbae2982f1c92ecca9c54227b20c2b35bcc89a5ab53bd60e946ee4d4d6a09ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Apatites - chemistry</topic><topic>Bone Remodeling</topic><topic>Crystallography, X-Ray</topic><topic>Femur - drug effects</topic><topic>Femur - metabolism</topic><topic>Isoflavones - pharmacology</topic><topic>Male</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Space life sciences</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ghezzo, C</creatorcontrib><creatorcontrib>Civitelli, R</creatorcontrib><creatorcontrib>Cadel, S</creatorcontrib><creatorcontrib>Borelli, G</creatorcontrib><creatorcontrib>Maiorino, M</creatorcontrib><creatorcontrib>Bufalino, L</creatorcontrib><creatorcontrib>Bongrani, S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Calcified tissue international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ghezzo, C</au><au>Civitelli, R</au><au>Cadel, S</au><au>Borelli, G</au><au>Maiorino, M</au><au>Bufalino, L</au><au>Bongrani, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ipriflavone does not alter bone apatite crystal structure in adult male rats</atitle><jtitle>Calcified tissue international</jtitle><addtitle>Calcif Tissue Int</addtitle><date>1996-12-01</date><risdate>1996</risdate><volume>59</volume><issue>6</issue><spage>496</spage><epage>499</epage><pages>496-499</pages><issn>0171-967X</issn><eissn>1432-0827</eissn><abstract>We have previously found that a short-term treatment with high doses of ipriflavone increased bone density and improved the biomechanical properties of adult male rat bones, without altering their mineral composition. To determine whether this effect can be associated with alterations of bone crystal structure, we have performed X-ray diffraction analysis of bones obtained from rats treated with ipriflavone at doses that were effective in inducing favorable changes on bone density and biomechanics. Eighteen-week-old male Sprague Dawley rats were treated by oral route with either ipriflavone (200 or 400 mg/kg/day), or its vehicle for 12 weeks. The treatment was well tolerated and body weight increased to the same extent in all animals. As a measure of bone crystallinity, we examined the (310) and (002) reflections of the X-ray diffraction patterns, corresponding to the directions perpendicular and parallel to the c-axis of the crystals, respectively. No major differences were observed between ipriflavone-treated and control animals for the broadening parameter beta(1/2) for (310) and (002) peaks, as well as for lattice parameters. Therefore, a 12-week treatment with ipriflavone at high doses does not induce significant modifications of bone "crystallinity." Thus, the positive effect of ipriflavone on bone mineral density appears to be associated with an increased apatite crystal formation rather than an increase of crystal size. These results provide further evidence for the safety and usefulness of ipriflavone in the treatment of osteoporotic syndromes.</abstract><cop>United States</cop><pmid>8939778</pmid><doi>10.1007/BF00369217</doi><tpages>4</tpages></addata></record> |
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subjects | Animals Apatites - chemistry Bone Remodeling Crystallography, X-Ray Femur - drug effects Femur - metabolism Isoflavones - pharmacology Male Rats Rats, Sprague-Dawley Space life sciences |
title | Ipriflavone does not alter bone apatite crystal structure in adult male rats |
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