Separation of Functional West Nile Virus Replication Complexes from Intracellular Membrane Fragments
The Wistar Institute, 36th and Spruce Streets, Philadelphia, Pennsylvania 19104, U.S.A. Flaviviruses encode seven non-structural proteins for which functions have not yet been described. The identification of the viral and possible host proteins which may be involved in flavivirus replication has be...
Gespeichert in:
| Veröffentlicht in: | Journal of general virology 1988-12, Vol.69 (12), p.3121-3127 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3127 |
|---|---|
| container_issue | 12 |
| container_start_page | 3121 |
| container_title | Journal of general virology |
| container_volume | 69 |
| creator | Grun, Janet B Brinton, Margo A |
| description | The Wistar Institute, 36th and Spruce Streets, Philadelphia, Pennsylvania 19104, U.S.A.
Flaviviruses encode seven non-structural proteins for which functions have not yet been described. The identification of the viral and possible host proteins which may be involved in flavivirus replication has been impeded by the fact that the viral replication complexes are tightly associated with endoplasmic reticular membranes within infected cells and that in vitro polymerase activity is associated with large membrane fragments. To facilitate further study of flavivirus replication complexes, selected ultrapure detergents were analysed for their effect on West Nile virus (WNV) in vitro RNA-dependent RNA polymerase activity and for their ability to release functional replication complexes from partially purified intracellular BHK-21 membrane fragments. A few previous reports indicated that flavivirus in vitro polymerase activity was sensitive to detergent treatment. The present study indicates that WNV polymerase activity is variably inhibited depending on the concentration and identity of the detergent used. Of the five detergents (Tween 20, maltoside, octylglucoside, lubrol PX and sodium deoxycholate) tested, sodium deoxycholate was the most efficient at releasing functional viral replication complexes from intracellular membranes.
Keywords: West Nile virus, replication complexes, RDRP
Received 19 April 1988;
accepted 5 September 1988. |
| doi_str_mv | 10.1099/0022-1317-69-12-3121 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78566586</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>78566586</sourcerecordid><originalsourceid>FETCH-LOGICAL-c442t-73a8771ab6bb12377cc5edbc1bd7c0aa70242fc3cf9b5084ccc2fbf59716a2b43</originalsourceid><addsrcrecordid>eNqFkVuLFDEQhYMo6-zqP1DIgwg-tKbSuXQeZXB0YVXw-hiSTDIbSV9Mur38-00zw-CbT1VQ36k6nELoCZCXQJR6RQilDbQgG6EaoE0LFO6hDTDBG1qB-2hzRh6iy1J-EAKMcXmBLlpQCki7QfvPfjLZzHEc8Bjwbhnc2puEv_sy4w8xefwt5qXgT35K0R3J7dhPyf_xBYc89vh6mLNxPqUlmYzf-95mM3i8y-bQ-2Euj9CDYFLxj0_1Cn3dvfmyfdfcfHx7vX190zjG6NzI1nRSgrHCWqCtlM5xv7cO7F46YowklNHgWheU5aRjzjkabOBKgjDUsvYKPT_unfL4c6n-dR_L6qu6GZeiZceF4J34LwicdiAEVJAdQZfHUrIPesqxN_mvBqLXL-g1Yr1GrIXSQPX6hSp7etq_2N7vz6JT7HX-7DQ3xZkUalwuljMmKRecqYq9OGK38XD7O2avD37oY_Vi46h_xfzPyTtlkJ7s</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15281661</pqid></control><display><type>article</type><title>Separation of Functional West Nile Virus Replication Complexes from Intracellular Membrane Fragments</title><source>MEDLINE</source><source>Free E-Journal (出版社公開部分のみ）</source><source>Microbiology Society</source><source>Alma/SFX Local Collection</source><creator>Grun, Janet B ; Brinton, Margo A</creator><creatorcontrib>Grun, Janet B ; Brinton, Margo A</creatorcontrib><description>The Wistar Institute, 36th and Spruce Streets, Philadelphia, Pennsylvania 19104, U.S.A.
Flaviviruses encode seven non-structural proteins for which functions have not yet been described. The identification of the viral and possible host proteins which may be involved in flavivirus replication has been impeded by the fact that the viral replication complexes are tightly associated with endoplasmic reticular membranes within infected cells and that in vitro polymerase activity is associated with large membrane fragments. To facilitate further study of flavivirus replication complexes, selected ultrapure detergents were analysed for their effect on West Nile virus (WNV) in vitro RNA-dependent RNA polymerase activity and for their ability to release functional replication complexes from partially purified intracellular BHK-21 membrane fragments. A few previous reports indicated that flavivirus in vitro polymerase activity was sensitive to detergent treatment. The present study indicates that WNV polymerase activity is variably inhibited depending on the concentration and identity of the detergent used. Of the five detergents (Tween 20, maltoside, octylglucoside, lubrol PX and sodium deoxycholate) tested, sodium deoxycholate was the most efficient at releasing functional viral replication complexes from intracellular membranes.
Keywords: West Nile virus, replication complexes, RDRP
Received 19 April 1988;
accepted 5 September 1988.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-69-12-3121</identifier><identifier>PMID: 3199103</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Animals ; Biological and medical sciences ; Cell Fractionation - methods ; Cells, Cultured ; Detergents - pharmacology ; DNA-Directed RNA Polymerases - antagonists & inhibitors ; Electrophoresis, Polyacrylamide Gel ; Endoplasmic Reticulum - microbiology ; Fundamental and applied biological sciences. Psychology ; Intracellular Membranes - microbiology ; Membrane Proteins - isolation & purification ; Microbiology ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; Surface-Active Agents - pharmacology ; Viral Proteins - isolation & purification ; Virology ; Virus Replication ; West Nile virus</subject><ispartof>Journal of general virology, 1988-12, Vol.69 (12), p.3121-3127</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-73a8771ab6bb12377cc5edbc1bd7c0aa70242fc3cf9b5084ccc2fbf59716a2b43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3733,3734,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7256549$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3199103$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Grun, Janet B</creatorcontrib><creatorcontrib>Brinton, Margo A</creatorcontrib><title>Separation of Functional West Nile Virus Replication Complexes from Intracellular Membrane Fragments</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>The Wistar Institute, 36th and Spruce Streets, Philadelphia, Pennsylvania 19104, U.S.A.
Flaviviruses encode seven non-structural proteins for which functions have not yet been described. The identification of the viral and possible host proteins which may be involved in flavivirus replication has been impeded by the fact that the viral replication complexes are tightly associated with endoplasmic reticular membranes within infected cells and that in vitro polymerase activity is associated with large membrane fragments. To facilitate further study of flavivirus replication complexes, selected ultrapure detergents were analysed for their effect on West Nile virus (WNV) in vitro RNA-dependent RNA polymerase activity and for their ability to release functional replication complexes from partially purified intracellular BHK-21 membrane fragments. A few previous reports indicated that flavivirus in vitro polymerase activity was sensitive to detergent treatment. The present study indicates that WNV polymerase activity is variably inhibited depending on the concentration and identity of the detergent used. Of the five detergents (Tween 20, maltoside, octylglucoside, lubrol PX and sodium deoxycholate) tested, sodium deoxycholate was the most efficient at releasing functional viral replication complexes from intracellular membranes.
Keywords: West Nile virus, replication complexes, RDRP
Received 19 April 1988;
accepted 5 September 1988.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Fractionation - methods</subject><subject>Cells, Cultured</subject><subject>Detergents - pharmacology</subject><subject>DNA-Directed RNA Polymerases - antagonists & inhibitors</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Endoplasmic Reticulum - microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Intracellular Membranes - microbiology</subject><subject>Membrane Proteins - isolation & purification</subject><subject>Microbiology</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>Surface-Active Agents - pharmacology</subject><subject>Viral Proteins - isolation & purification</subject><subject>Virology</subject><subject>Virus Replication</subject><subject>West Nile virus</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVuLFDEQhYMo6-zqP1DIgwg-tKbSuXQeZXB0YVXw-hiSTDIbSV9Mur38-00zw-CbT1VQ36k6nELoCZCXQJR6RQilDbQgG6EaoE0LFO6hDTDBG1qB-2hzRh6iy1J-EAKMcXmBLlpQCki7QfvPfjLZzHEc8Bjwbhnc2puEv_sy4w8xefwt5qXgT35K0R3J7dhPyf_xBYc89vh6mLNxPqUlmYzf-95mM3i8y-bQ-2Euj9CDYFLxj0_1Cn3dvfmyfdfcfHx7vX190zjG6NzI1nRSgrHCWqCtlM5xv7cO7F46YowklNHgWheU5aRjzjkabOBKgjDUsvYKPT_unfL4c6n-dR_L6qu6GZeiZceF4J34LwicdiAEVJAdQZfHUrIPesqxN_mvBqLXL-g1Yr1GrIXSQPX6hSp7etq_2N7vz6JT7HX-7DQ3xZkUalwuljMmKRecqYq9OGK38XD7O2avD37oY_Vi46h_xfzPyTtlkJ7s</recordid><startdate>19881201</startdate><enddate>19881201</enddate><creator>Grun, Janet B</creator><creator>Brinton, Margo A</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19881201</creationdate><title>Separation of Functional West Nile Virus Replication Complexes from Intracellular Membrane Fragments</title><author>Grun, Janet B ; Brinton, Margo A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-73a8771ab6bb12377cc5edbc1bd7c0aa70242fc3cf9b5084ccc2fbf59716a2b43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Fractionation - methods</topic><topic>Cells, Cultured</topic><topic>Detergents - pharmacology</topic><topic>DNA-Directed RNA Polymerases - antagonists & inhibitors</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Endoplasmic Reticulum - microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Intracellular Membranes - microbiology</topic><topic>Membrane Proteins - isolation & purification</topic><topic>Microbiology</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>Surface-Active Agents - pharmacology</topic><topic>Viral Proteins - isolation & purification</topic><topic>Virology</topic><topic>Virus Replication</topic><topic>West Nile virus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Grun, Janet B</creatorcontrib><creatorcontrib>Brinton, Margo A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Grun, Janet B</au><au>Brinton, Margo A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Separation of Functional West Nile Virus Replication Complexes from Intracellular Membrane Fragments</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1988-12-01</date><risdate>1988</risdate><volume>69</volume><issue>12</issue><spage>3121</spage><epage>3127</epage><pages>3121-3127</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>The Wistar Institute, 36th and Spruce Streets, Philadelphia, Pennsylvania 19104, U.S.A.
Flaviviruses encode seven non-structural proteins for which functions have not yet been described. The identification of the viral and possible host proteins which may be involved in flavivirus replication has been impeded by the fact that the viral replication complexes are tightly associated with endoplasmic reticular membranes within infected cells and that in vitro polymerase activity is associated with large membrane fragments. To facilitate further study of flavivirus replication complexes, selected ultrapure detergents were analysed for their effect on West Nile virus (WNV) in vitro RNA-dependent RNA polymerase activity and for their ability to release functional replication complexes from partially purified intracellular BHK-21 membrane fragments. A few previous reports indicated that flavivirus in vitro polymerase activity was sensitive to detergent treatment. The present study indicates that WNV polymerase activity is variably inhibited depending on the concentration and identity of the detergent used. Of the five detergents (Tween 20, maltoside, octylglucoside, lubrol PX and sodium deoxycholate) tested, sodium deoxycholate was the most efficient at releasing functional viral replication complexes from intracellular membranes.
Keywords: West Nile virus, replication complexes, RDRP
Received 19 April 1988;
accepted 5 September 1988.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>3199103</pmid><doi>10.1099/0022-1317-69-12-3121</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1317 |
| ispartof | Journal of general virology, 1988-12, Vol.69 (12), p.3121-3127 |
| issn | 0022-1317 1465-2099 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78566586 |
| source | MEDLINE; Free E-Journal (出版社公開部分のみ）; Microbiology Society; Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences Cell Fractionation - methods Cells, Cultured Detergents - pharmacology DNA-Directed RNA Polymerases - antagonists & inhibitors Electrophoresis, Polyacrylamide Gel Endoplasmic Reticulum - microbiology Fundamental and applied biological sciences. Psychology Intracellular Membranes - microbiology Membrane Proteins - isolation & purification Microbiology Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Surface-Active Agents - pharmacology Viral Proteins - isolation & purification Virology Virus Replication West Nile virus |
| title | Separation of Functional West Nile Virus Replication Complexes from Intracellular Membrane Fragments |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-04T14%3A00%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Separation%20of%20Functional%20West%20Nile%20Virus%20Replication%20Complexes%20from%20Intracellular%20Membrane%20Fragments&rft.jtitle=Journal%20of%20general%20virology&rft.au=Grun,%20Janet%20B&rft.date=1988-12-01&rft.volume=69&rft.issue=12&rft.spage=3121&rft.epage=3127&rft.pages=3121-3127&rft.issn=0022-1317&rft.eissn=1465-2099&rft.coden=JGVIAY&rft_id=info:doi/10.1099/0022-1317-69-12-3121&rft_dat=%3Cproquest_cross%3E78566586%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15281661&rft_id=info:pmid/3199103&rfr_iscdi=true |