Evidence for Multiple Opioid Receptors in the Human Posterior Pituitary
The distribution and quantification of opioid receptor types in post‐mortem human pituitary cryostat sections was determined by quantitative in vitro receptor autoradiography. Highly specific radioligands were used for each opioid receptor type, i.e. [125I]‐FK‐33‐824 for μ‐opioid sites, [125I][D.Ala...
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| Veröffentlicht in: | Journal of neuroendocrinology 1996-11, Vol.8 (11), p.883-887 |
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| container_title | Journal of neuroendocrinology |
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| creator | Jordan, D. Tafani, J. A. M. Ries, C. Zajac, J. M. Simonnet, G. Martin, D. Kopp, N. Allard, M. |
| description | The distribution and quantification of opioid receptor types in post‐mortem human pituitary cryostat sections was determined by quantitative in vitro receptor autoradiography. Highly specific radioligands were used for each opioid receptor type, i.e. [125I]‐FK‐33‐824 for μ‐opioid sites, [125I][D.Ala2]‐Deltorphin‐I for δ‐opioid sites and 3H‐U69,593 for κ‐opioid sites.
None of the five specimens tested exhibited any labelling in the anterior lobe of the pituitary for the three radioligands. As for the posterior pituitary, all 5 specimens contained both μ and κ‐opioid binding sites whereas none of them showed δ‐binding sites.
The presence of both μ‐ and κ‐opioid binding sites in the human posterior pituitary contrasts with previous findings in the rat where only κ‐sites have been found. The present study could contribute to understanding of the functional action of opioids in the human pituitary. |
| doi_str_mv | 10.1046/j.1365-2826.1996.53210.x |
| format | Article |
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None of the five specimens tested exhibited any labelling in the anterior lobe of the pituitary for the three radioligands. As for the posterior pituitary, all 5 specimens contained both μ and κ‐opioid binding sites whereas none of them showed δ‐binding sites.
The presence of both μ‐ and κ‐opioid binding sites in the human posterior pituitary contrasts with previous findings in the rat where only κ‐sites have been found. The present study could contribute to understanding of the functional action of opioids in the human pituitary.</description><identifier>ISSN: 0953-8194</identifier><identifier>EISSN: 1365-2826</identifier><identifier>DOI: 10.1046/j.1365-2826.1996.53210.x</identifier><identifier>PMID: 8933366</identifier><language>eng</language><publisher>Oxford: Blackwell Science Ltd</publisher><subject>3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer ; Adult ; Benzeneacetamides ; Binding Sites ; Binding, Competitive ; Cadaver ; D-Ala,MePhe,Met-ol-enkephalin - metabolism ; Enkephalin, Ala-MePhe-Gly ; Enkephalins - pharmacology ; Female ; human ; Humans ; Male ; Middle Aged ; Narcotics - metabolism ; Oligopeptides - pharmacology ; opioid receptors ; pituitary ; Pituitary Gland, Posterior - metabolism ; Pyrrolidines - metabolism ; Receptors, Opioid - metabolism ; Tissue Distribution</subject><ispartof>Journal of neuroendocrinology, 1996-11, Vol.8 (11), p.883-887</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4012-3f0980cee557d93c7770caeef59c0218dfbd948dbf41c5e8c808b01036a569373</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2826.1996.53210.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2826.1996.53210.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8933366$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jordan, D.</creatorcontrib><creatorcontrib>Tafani, J. A. M.</creatorcontrib><creatorcontrib>Ries, C.</creatorcontrib><creatorcontrib>Zajac, J. M.</creatorcontrib><creatorcontrib>Simonnet, G.</creatorcontrib><creatorcontrib>Martin, D.</creatorcontrib><creatorcontrib>Kopp, N.</creatorcontrib><creatorcontrib>Allard, M.</creatorcontrib><title>Evidence for Multiple Opioid Receptors in the Human Posterior Pituitary</title><title>Journal of neuroendocrinology</title><addtitle>J Neuroendocrinol</addtitle><description>The distribution and quantification of opioid receptor types in post‐mortem human pituitary cryostat sections was determined by quantitative in vitro receptor autoradiography. Highly specific radioligands were used for each opioid receptor type, i.e. [125I]‐FK‐33‐824 for μ‐opioid sites, [125I][D.Ala2]‐Deltorphin‐I for δ‐opioid sites and 3H‐U69,593 for κ‐opioid sites.
None of the five specimens tested exhibited any labelling in the anterior lobe of the pituitary for the three radioligands. As for the posterior pituitary, all 5 specimens contained both μ and κ‐opioid binding sites whereas none of them showed δ‐binding sites.
The presence of both μ‐ and κ‐opioid binding sites in the human posterior pituitary contrasts with previous findings in the rat where only κ‐sites have been found. The present study could contribute to understanding of the functional action of opioids in the human pituitary.</description><subject>3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer</subject><subject>Adult</subject><subject>Benzeneacetamides</subject><subject>Binding Sites</subject><subject>Binding, Competitive</subject><subject>Cadaver</subject><subject>D-Ala,MePhe,Met-ol-enkephalin - metabolism</subject><subject>Enkephalin, Ala-MePhe-Gly</subject><subject>Enkephalins - pharmacology</subject><subject>Female</subject><subject>human</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Narcotics - metabolism</subject><subject>Oligopeptides - pharmacology</subject><subject>opioid receptors</subject><subject>pituitary</subject><subject>Pituitary Gland, Posterior - metabolism</subject><subject>Pyrrolidines - metabolism</subject><subject>Receptors, Opioid - metabolism</subject><subject>Tissue Distribution</subject><issn>0953-8194</issn><issn>1365-2826</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtOwzAQRS0EgvL4BCSv2KXYcfyS2KBSyrNUCMTSSp2JcEmbYCdQ_h6XVl2zmpHunJnRQQhT0qckE-ezPmWCJ6lKRZ9qLfqcpTFb7qDeNthFPaI5SxTV2QE6DGFGCJWckX20rzRjTIgeGg2_XAELC7isPX7sqtY1FeCnxtWuwM9goWlrH7Bb4PYd8E03zxd4UocWvIvAxLWda3P_c4z2yrwKcLKpR-j1evgyuEkenka3g8uHxGaEpgkriVbEAnAuC82slJLYHKDk2pKUqqKcFjpTxbTMqOWgrCJqSihhIudCM8mO0Nl6b-Przw5Ca-YuWKiqfAF1F4xUXDCh0jio1oPW1yF4KE3j3Tx-aigxK4dmZlaqzEqVWTk0fw7NMqKnmxvddA7FFtxIi_nFOv92Ffz8e6-5Gw9jG_FkjbtocbnFc_9hhGSSm7fxyFxNRuPr-7eBEewXKfOOtw</recordid><startdate>199611</startdate><enddate>199611</enddate><creator>Jordan, D.</creator><creator>Tafani, J. A. M.</creator><creator>Ries, C.</creator><creator>Zajac, J. M.</creator><creator>Simonnet, G.</creator><creator>Martin, D.</creator><creator>Kopp, N.</creator><creator>Allard, M.</creator><general>Blackwell Science Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199611</creationdate><title>Evidence for Multiple Opioid Receptors in the Human Posterior Pituitary</title><author>Jordan, D. ; Tafani, J. A. M. ; Ries, C. ; Zajac, J. M. ; Simonnet, G. ; Martin, D. ; Kopp, N. ; Allard, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4012-3f0980cee557d93c7770caeef59c0218dfbd948dbf41c5e8c808b01036a569373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer</topic><topic>Adult</topic><topic>Benzeneacetamides</topic><topic>Binding Sites</topic><topic>Binding, Competitive</topic><topic>Cadaver</topic><topic>D-Ala,MePhe,Met-ol-enkephalin - metabolism</topic><topic>Enkephalin, Ala-MePhe-Gly</topic><topic>Enkephalins - pharmacology</topic><topic>Female</topic><topic>human</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Narcotics - metabolism</topic><topic>Oligopeptides - pharmacology</topic><topic>opioid receptors</topic><topic>pituitary</topic><topic>Pituitary Gland, Posterior - metabolism</topic><topic>Pyrrolidines - metabolism</topic><topic>Receptors, Opioid - metabolism</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jordan, D.</creatorcontrib><creatorcontrib>Tafani, J. A. M.</creatorcontrib><creatorcontrib>Ries, C.</creatorcontrib><creatorcontrib>Zajac, J. M.</creatorcontrib><creatorcontrib>Simonnet, G.</creatorcontrib><creatorcontrib>Martin, D.</creatorcontrib><creatorcontrib>Kopp, N.</creatorcontrib><creatorcontrib>Allard, M.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroendocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jordan, D.</au><au>Tafani, J. A. M.</au><au>Ries, C.</au><au>Zajac, J. M.</au><au>Simonnet, G.</au><au>Martin, D.</au><au>Kopp, N.</au><au>Allard, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence for Multiple Opioid Receptors in the Human Posterior Pituitary</atitle><jtitle>Journal of neuroendocrinology</jtitle><addtitle>J Neuroendocrinol</addtitle><date>1996-11</date><risdate>1996</risdate><volume>8</volume><issue>11</issue><spage>883</spage><epage>887</epage><pages>883-887</pages><issn>0953-8194</issn><eissn>1365-2826</eissn><abstract>The distribution and quantification of opioid receptor types in post‐mortem human pituitary cryostat sections was determined by quantitative in vitro receptor autoradiography. Highly specific radioligands were used for each opioid receptor type, i.e. [125I]‐FK‐33‐824 for μ‐opioid sites, [125I][D.Ala2]‐Deltorphin‐I for δ‐opioid sites and 3H‐U69,593 for κ‐opioid sites.
None of the five specimens tested exhibited any labelling in the anterior lobe of the pituitary for the three radioligands. As for the posterior pituitary, all 5 specimens contained both μ and κ‐opioid binding sites whereas none of them showed δ‐binding sites.
The presence of both μ‐ and κ‐opioid binding sites in the human posterior pituitary contrasts with previous findings in the rat where only κ‐sites have been found. The present study could contribute to understanding of the functional action of opioids in the human pituitary.</abstract><cop>Oxford</cop><pub>Blackwell Science Ltd</pub><pmid>8933366</pmid><doi>10.1046/j.1365-2826.1996.53210.x</doi><tpages>5</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | 3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer Adult Benzeneacetamides Binding Sites Binding, Competitive Cadaver D-Ala,MePhe,Met-ol-enkephalin - metabolism Enkephalin, Ala-MePhe-Gly Enkephalins - pharmacology Female human Humans Male Middle Aged Narcotics - metabolism Oligopeptides - pharmacology opioid receptors pituitary Pituitary Gland, Posterior - metabolism Pyrrolidines - metabolism Receptors, Opioid - metabolism Tissue Distribution |
| title | Evidence for Multiple Opioid Receptors in the Human Posterior Pituitary |
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