Dynamics of gene transfer to retinal pigment epithelium

To examine the nature and dynamics of gene transfer to human retinal pigment epithelium (RPE) using an adenoviral vector and adjuvants that may enhance the uptake of recombinant adenoviruses. Human RPE cultures (HRPE7) were transfected in vitro with varying concentrations (4, 20, 40, 120, and 200 pf...

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Veröffentlicht in:	Investigative ophthalmology & visual science 1996-11, Vol.37 (12), p.2447-2454
Hauptverfasser:	da Cruz, L, Rakoczy, P, Perricaudet, M, Constable, IJ
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenoviruses, Human - genetics beta-Galactosidase - genetics beta-Galactosidase - metabolism Biological and medical sciences Biotechnology Child Defective Viruses Fibroblasts - cytology Fibroblasts - drug effects Fibroblasts - enzymology Fibroblasts - virology Fluorescent Antibody Technique, Indirect Fundamental and applied biological sciences. Psychology Gene Expression Genetic engineering Genetic technics Genetic Vectors Humans Hyaluronan Receptors - metabolism Hyaluronic Acid - pharmacology Hyaluronic Acid - physiology Intercellular Adhesion Molecule-1 - metabolism Lung - cytology Lung - drug effects Lung - enzymology Lung - virology Methods. Procedures. Technologies Middle Aged Pigment Epithelium of Eye - cytology Pigment Epithelium of Eye - drug effects Pigment Epithelium of Eye - enzymology Pigment Epithelium of Eye - virology Transfection Vectors (cloning, transfer, expression). Insertion sequences and transposons
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