Direct 99mTc-labeling of antibodies by sodium dithionite reduction, and role of ascorbate as a stabilizer in cysteine challenge

A method for the direct 99mTc-labeling of antibodies by dithionite reduction was developed. Among three murine monoclonal IgG1 and one human polyclonal IgG (hIgG) antibodies tested, hIgG was the most quickly reduced by dithionite. These differences may reflect the reactivities of antibody disulfide...

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Veröffentlicht in:	Nuclear medicine and biology 1996-08, Vol.23 (6), p.827-835
Hauptverfasser:	Qi, P., Muddukrishna, S.N., Torok-Both, R., Rahn, J., Chen, A.
Format:	Artikel
Sprache:	eng
Schlagworte:	99mTc Animals Antibodies, Monoclonal - chemistry Ascorbate Ascorbic Acid - chemistry Chromatography, High Pressure Liquid Cysteine - chemistry Direct labeling Dithionite Dithionite - chemistry Excipients - chemistry Humans Immunoglobulin G - chemistry Isotope Labeling - methods Mice Monoclonal antibody Oxidation-Reduction Sulfhydryl Compounds - chemistry Technetium Compounds - chemical synthesis
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