Expression and Regulation of c-erb-A mRNA from Lymphocytes in Patients with Thyroid Dysfunction
To investigate the expression and the regulation of nuclear triiodothyronine (T3) receptor at the gene level, cellular (c)-erb-A mRNA isolated from lymphocytes in patients with thyroid dysfunction was examined by Northern gel analysis and dot blot hybridization using viral (v)-erb-A cDNA probe. Huma...
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| Veröffentlicht in: | Endocrinologia Japonica 1988, Vol.35(3), pp.463-467 |
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| container_title | Endocrinologia Japonica |
| container_volume | 35 |
| creator | NAGAYAMA, YUJI YAMASHITA, SHUNICHI HIRAYU, HIDESHI ASHIZAWA, KIYOTO HARAKAWA, SEIJIRO INOUE, SHUJI IZUMI, MOTOMORI NAGATAKI, SHIGENOBU |
| description | To investigate the expression and the regulation of nuclear triiodothyronine (T3) receptor at the gene level, cellular (c)-erb-A mRNA isolated from lymphocytes in patients with thyroid dysfunction was examined by Northern gel analysis and dot blot hybridization using viral (v)-erb-A cDNA probe. Human lymphocytes contained c-erb-A mRNA (approximately 2.0 kilobase (kb) in length), and c-erb-A mRNA, which was determined by dot blot hybridization, was observed to be increased in hypothyroid patients but unaltered in hyperthyroid patients. The high level of c-erb-A mRNA may contribute in part to the increase in nuclear T3 receptor and these results suggest the presence of up-regulation of nuclear T3 receptor at the gene level in the lymphocytes of hypothyroid patients. |
| doi_str_mv | 10.1507/endocrj1954.35.463 |
| format | Article |
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Human lymphocytes contained c-erb-A mRNA (approximately 2.0 kilobase (kb) in length), and c-erb-A mRNA, which was determined by dot blot hybridization, was observed to be increased in hypothyroid patients but unaltered in hyperthyroid patients. The high level of c-erb-A mRNA may contribute in part to the increase in nuclear T3 receptor and these results suggest the presence of up-regulation of nuclear T3 receptor at the gene level in the lymphocytes of hypothyroid patients.</description><identifier>ISSN: 0013-7219</identifier><identifier>EISSN: 2185-6370</identifier><identifier>DOI: 10.1507/endocrj1954.35.463</identifier><identifier>PMID: 3197657</identifier><identifier>CODEN: ECJPAE</identifier><language>eng</language><publisher>Tokyo: The Japan Endocrine Society</publisher><subject>Biological and medical sciences ; Cell Nucleus - analysis ; Cell Nucleus - ultrastructure ; DNA Probes - analysis ; DNA, Viral - analysis ; Female genital diseases ; Gene Expression Regulation ; Gynecology. Andrology. Obstetrics ; Humans ; Hypothyroidism - genetics ; Lymphocytes - metabolism ; Lymphocytes - ultrastructure ; Medical sciences ; Receptors, Thyroid Hormone - analysis ; Receptors, Thyroid Hormone - genetics ; RNA, Messenger - genetics ; Thyroid Gland - metabolism ; Thyroid Gland - physiopathology ; Tumors</subject><ispartof>Endocrinologia Japonica, 1988, Vol.35(3), pp.463-467</ispartof><rights>The Japan Endocrine Society</rights><rights>1990 INIST-CNRS</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4163-a48f5f29cba8a521d13dd3a651dd0fb214469da8d2b31c157c950a571644107b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1882,4023,27922,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6752128$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3197657$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NAGAYAMA, YUJI</creatorcontrib><creatorcontrib>YAMASHITA, SHUNICHI</creatorcontrib><creatorcontrib>HIRAYU, HIDESHI</creatorcontrib><creatorcontrib>ASHIZAWA, KIYOTO</creatorcontrib><creatorcontrib>HARAKAWA, SEIJIRO</creatorcontrib><creatorcontrib>INOUE, SHUJI</creatorcontrib><creatorcontrib>IZUMI, MOTOMORI</creatorcontrib><creatorcontrib>NAGATAKI, SHIGENOBU</creatorcontrib><title>Expression and Regulation of c-erb-A mRNA from Lymphocytes in Patients with Thyroid Dysfunction</title><title>Endocrinologia Japonica</title><addtitle>Endocrinol Japon</addtitle><description>To investigate the expression and the regulation of nuclear triiodothyronine (T3) receptor at the gene level, cellular (c)-erb-A mRNA isolated from lymphocytes in patients with thyroid dysfunction was examined by Northern gel analysis and dot blot hybridization using viral (v)-erb-A cDNA probe. Human lymphocytes contained c-erb-A mRNA (approximately 2.0 kilobase (kb) in length), and c-erb-A mRNA, which was determined by dot blot hybridization, was observed to be increased in hypothyroid patients but unaltered in hyperthyroid patients. The high level of c-erb-A mRNA may contribute in part to the increase in nuclear T3 receptor and these results suggest the presence of up-regulation of nuclear T3 receptor at the gene level in the lymphocytes of hypothyroid patients.</description><subject>Biological and medical sciences</subject><subject>Cell Nucleus - analysis</subject><subject>Cell Nucleus - ultrastructure</subject><subject>DNA Probes - analysis</subject><subject>DNA, Viral - analysis</subject><subject>Female genital diseases</subject><subject>Gene Expression Regulation</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Hypothyroidism - genetics</subject><subject>Lymphocytes - metabolism</subject><subject>Lymphocytes - ultrastructure</subject><subject>Medical sciences</subject><subject>Receptors, Thyroid Hormone - analysis</subject><subject>Receptors, Thyroid Hormone - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>Thyroid Gland - metabolism</subject><subject>Thyroid Gland - physiopathology</subject><subject>Tumors</subject><issn>0013-7219</issn><issn>2185-6370</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkF1r2zAUhsXY6ELXPzAY6GLszpmOPu3L0HVtIWyjdNdC1kfjYFuZZLP5308hIYzp4gjxPuc9Oi9C74GsQRD12Y8u2rSHRvA1E2su2Su0olCLSjJFXqMVIcAqRaF5i25y3pNyJJWKkyt0xaBRUqgV0nd_Dsnn3MURm9HhJ_8y92Y6PmPAtvKprTZ4ePq2wSHFAW-X4bCLdpl8xt2IfxTUj1PGv7tph593S4qdw1-WHObRHl3eoTfB9NnfnO9r9PPr3fPtQ7X9fv94u9lWloNkleF1EIE2tjW1ERQcMOeYkQKcI6GlwLlsnKkdbRlYEMo2ghihQHIORLXsGn06-R5S_DX7POmhy9b3vRl9nLNWtZBECCggPYE2xZyTD_qQusGkRQPRx2D1P8FqJnQJtjR9OLvP7eDdpeUcY9E_nnWTrelDMqPt8gWTqqxE64Ldn7B9nsyLv-gmTZ3t_f-T2amUD1wIuzOpYOwvfjGbYw</recordid><startdate>1988</startdate><enddate>1988</enddate><creator>NAGAYAMA, YUJI</creator><creator>YAMASHITA, SHUNICHI</creator><creator>HIRAYU, HIDESHI</creator><creator>ASHIZAWA, KIYOTO</creator><creator>HARAKAWA, SEIJIRO</creator><creator>INOUE, SHUJI</creator><creator>IZUMI, MOTOMORI</creator><creator>NAGATAKI, SHIGENOBU</creator><general>The Japan Endocrine Society</general><general>Japan Endocrine Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1988</creationdate><title>Expression and Regulation of c-erb-A mRNA from Lymphocytes in Patients with Thyroid Dysfunction</title><author>NAGAYAMA, YUJI ; YAMASHITA, SHUNICHI ; HIRAYU, HIDESHI ; ASHIZAWA, KIYOTO ; HARAKAWA, SEIJIRO ; INOUE, SHUJI ; IZUMI, MOTOMORI ; NAGATAKI, SHIGENOBU</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4163-a48f5f29cba8a521d13dd3a651dd0fb214469da8d2b31c157c950a571644107b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Biological and medical sciences</topic><topic>Cell Nucleus - analysis</topic><topic>Cell Nucleus - ultrastructure</topic><topic>DNA Probes - analysis</topic><topic>DNA, Viral - analysis</topic><topic>Female genital diseases</topic><topic>Gene Expression Regulation</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Hypothyroidism - genetics</topic><topic>Lymphocytes - metabolism</topic><topic>Lymphocytes - ultrastructure</topic><topic>Medical sciences</topic><topic>Receptors, Thyroid Hormone - analysis</topic><topic>Receptors, Thyroid Hormone - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>Thyroid Gland - metabolism</topic><topic>Thyroid Gland - physiopathology</topic><topic>Tumors</topic><toplevel>online_resources</toplevel><creatorcontrib>NAGAYAMA, YUJI</creatorcontrib><creatorcontrib>YAMASHITA, SHUNICHI</creatorcontrib><creatorcontrib>HIRAYU, HIDESHI</creatorcontrib><creatorcontrib>ASHIZAWA, KIYOTO</creatorcontrib><creatorcontrib>HARAKAWA, SEIJIRO</creatorcontrib><creatorcontrib>INOUE, SHUJI</creatorcontrib><creatorcontrib>IZUMI, MOTOMORI</creatorcontrib><creatorcontrib>NAGATAKI, SHIGENOBU</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Endocrinologia Japonica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>NAGAYAMA, YUJI</au><au>YAMASHITA, SHUNICHI</au><au>HIRAYU, HIDESHI</au><au>ASHIZAWA, KIYOTO</au><au>HARAKAWA, SEIJIRO</au><au>INOUE, SHUJI</au><au>IZUMI, MOTOMORI</au><au>NAGATAKI, SHIGENOBU</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and Regulation of c-erb-A mRNA from Lymphocytes in Patients with Thyroid Dysfunction</atitle><jtitle>Endocrinologia Japonica</jtitle><addtitle>Endocrinol Japon</addtitle><date>1988</date><risdate>1988</risdate><volume>35</volume><issue>3</issue><spage>463</spage><epage>467</epage><pages>463-467</pages><issn>0013-7219</issn><eissn>2185-6370</eissn><coden>ECJPAE</coden><abstract>To investigate the expression and the regulation of nuclear triiodothyronine (T3) receptor at the gene level, cellular (c)-erb-A mRNA isolated from lymphocytes in patients with thyroid dysfunction was examined by Northern gel analysis and dot blot hybridization using viral (v)-erb-A cDNA probe. Human lymphocytes contained c-erb-A mRNA (approximately 2.0 kilobase (kb) in length), and c-erb-A mRNA, which was determined by dot blot hybridization, was observed to be increased in hypothyroid patients but unaltered in hyperthyroid patients. The high level of c-erb-A mRNA may contribute in part to the increase in nuclear T3 receptor and these results suggest the presence of up-regulation of nuclear T3 receptor at the gene level in the lymphocytes of hypothyroid patients.</abstract><cop>Tokyo</cop><pub>The Japan Endocrine Society</pub><pmid>3197657</pmid><doi>10.1507/endocrj1954.35.463</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Endocrinologia Japonica, 1988, Vol.35(3), pp.463-467 |
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| language | eng |
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| source | J-STAGE Free; MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Biological and medical sciences Cell Nucleus - analysis Cell Nucleus - ultrastructure DNA Probes - analysis DNA, Viral - analysis Female genital diseases Gene Expression Regulation Gynecology. Andrology. Obstetrics Humans Hypothyroidism - genetics Lymphocytes - metabolism Lymphocytes - ultrastructure Medical sciences Receptors, Thyroid Hormone - analysis Receptors, Thyroid Hormone - genetics RNA, Messenger - genetics Thyroid Gland - metabolism Thyroid Gland - physiopathology Tumors |
| title | Expression and Regulation of c-erb-A mRNA from Lymphocytes in Patients with Thyroid Dysfunction |
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