Molecular cloning and expression of the glucose/xylose isomerase gene from Streptomyces sp. NCIM 2730 in Escherichia coli

Abstract A partial genomic library of Streptomyces sp. NCIM 2730 was constructed in Escherichia coli using pUC8 vector and screened for the presence of the d-glucose/xylose isomerase (GXI) gene using an 18-mer mixed oligonucleotide probe complementary to a highly conserved six-amino acid sequence of...

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Veröffentlicht in:	FEMS microbiology letters 1996-11, Vol.145 (1), p.95-100
Hauptverfasser:	Bhosale, S.H., Ghatge, M.S., Deshpande, V.V.
Format:	Artikel
Sprache:	eng
Schlagworte:	Actinomycetes Aldose-Ketose Isomerases Amino acid sequence Amino acids Bacterial Proteins - genetics Bacterial Proteins - metabolism Blotting, Southern Blotting, Western Carbohydrate Epimerases - genetics Carbon sources Cloning Cloning, Molecular Conserved sequence E coli Escherichia coli Escherichia coli - genetics Expression Fusion protein Gene expression Gene Expression Regulation, Bacterial - physiology Gene Expression Regulation, Enzymologic - physiology Gene Library Genes, Bacterial - genetics Genetic Complementation Test Genetic Testing Glucose Glucose isomerase Glucose/xylose isomerase Microbiology Oligonucleotides pUC8 Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Restriction Mapping Streptomyces Streptomyces - enzymology Streptomyces - genetics Substrate Specificity Xylose Xylose isomerase
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