The cellular internalization of recombinant gamma interferon differs from that of natural interferon gamma
Purified natural and recombinant murine gamma interferons (MuIFN-γ) bind at 4°C to cultured L929 mouse fibroblasts with comparable receptor-binding affinity (Kd=9 × 10 −10M). Both 125I-labeled MuIFNs are rapidly internalized by cells at 37°C, although recombinant IFN is internalized somewhat more sl...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1988-11, Vol.157 (1), p.109-114 |
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| creator | Kushnaryov, Vladimir M. MacDonald, Hector S. Sedmak, J. James Grossberg, Sidney E. |
| description | Purified natural and recombinant murine gamma interferons (MuIFN-γ) bind at 4°C to cultured L929 mouse fibroblasts with comparable receptor-binding affinity (Kd=9 × 10
−10M). Both
125I-labeled MuIFNs are rapidly internalized by cells at 37°C, although recombinant IFN is internalized somewhat more slowly than natural IFN (t
1/2=90 sec and 45 sec, respectively). Immunoelectronmicroscopy showed that the majority of bound recombinant MuIFN-γ was located on the plasma membrane outside of coated areas, whereas natural interferon was found mainly in coated pits. At 37°C most of the recombinant molecules entered the cytoplasm in pinocytotic vesicles, while natural interferon was internalized by the specific mechanism of receptor-mediated endocytosis [1]. However, nearly equal amounts of immunocytochemically detectable molecules of both IFNs were found in the cell nucleus within 2–3 min incubation at 37°C. Thus, the process of translocation of the recombinant IFN-γ appears to differ from that of the natural product. |
| doi_str_mv | 10.1016/S0006-291X(88)80019-6 |
| format | Article |
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125I-labeled MuIFNs are rapidly internalized by cells at 37°C, although recombinant IFN is internalized somewhat more slowly than natural IFN (t
1/2=90 sec and 45 sec, respectively). Immunoelectronmicroscopy showed that the majority of bound recombinant MuIFN-γ was located on the plasma membrane outside of coated areas, whereas natural interferon was found mainly in coated pits. At 37°C most of the recombinant molecules entered the cytoplasm in pinocytotic vesicles, while natural interferon was internalized by the specific mechanism of receptor-mediated endocytosis [1]. However, nearly equal amounts of immunocytochemically detectable molecules of both IFNs were found in the cell nucleus within 2–3 min incubation at 37°C. Thus, the process of translocation of the recombinant IFN-γ appears to differ from that of the natural product.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/S0006-291X(88)80019-6</identifier><identifier>PMID: 2848515</identifier><identifier>CODEN: BBRCA9</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Analysis of the immune response. Humoral and cellular immunity ; Animals ; Biological and medical sciences ; Biological Transport ; Cell Compartmentation ; Cell Nucleus - metabolism ; Coated Pits, Cell-Membrane - metabolism ; Cytoplasm - metabolism ; Endocytosis ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Immunobiology ; In Vitro Techniques ; Interferon-gamma - pharmacokinetics ; L Cells (Cell Line) - metabolism ; Mice ; Miscellaneous ; Receptors, Cell Surface - metabolism ; Recombinant Proteins ; Regulatory factors and their cellular receptors</subject><ispartof>Biochemical and biophysical research communications, 1988-11, Vol.157 (1), p.109-114</ispartof><rights>1988 Academic Press, Inc.</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-a3196beb34e05914d7a296de933c3374f879ae46086358273783f38e035d94193</citedby><cites>FETCH-LOGICAL-c420t-a3196beb34e05914d7a296de933c3374f879ae46086358273783f38e035d94193</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-291X(88)80019-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6743340$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2848515$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kushnaryov, Vladimir M.</creatorcontrib><creatorcontrib>MacDonald, Hector S.</creatorcontrib><creatorcontrib>Sedmak, J. James</creatorcontrib><creatorcontrib>Grossberg, Sidney E.</creatorcontrib><title>The cellular internalization of recombinant gamma interferon differs from that of natural interferon gamma</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>Purified natural and recombinant murine gamma interferons (MuIFN-γ) bind at 4°C to cultured L929 mouse fibroblasts with comparable receptor-binding affinity (Kd=9 × 10
−10M). Both
125I-labeled MuIFNs are rapidly internalized by cells at 37°C, although recombinant IFN is internalized somewhat more slowly than natural IFN (t
1/2=90 sec and 45 sec, respectively). Immunoelectronmicroscopy showed that the majority of bound recombinant MuIFN-γ was located on the plasma membrane outside of coated areas, whereas natural interferon was found mainly in coated pits. At 37°C most of the recombinant molecules entered the cytoplasm in pinocytotic vesicles, while natural interferon was internalized by the specific mechanism of receptor-mediated endocytosis [1]. However, nearly equal amounts of immunocytochemically detectable molecules of both IFNs were found in the cell nucleus within 2–3 min incubation at 37°C. Thus, the process of translocation of the recombinant IFN-γ appears to differ from that of the natural product.</description><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Cell Compartmentation</subject><subject>Cell Nucleus - metabolism</subject><subject>Coated Pits, Cell-Membrane - metabolism</subject><subject>Cytoplasm - metabolism</subject><subject>Endocytosis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Immunobiology</subject><subject>In Vitro Techniques</subject><subject>Interferon-gamma - pharmacokinetics</subject><subject>L Cells (Cell Line) - metabolism</subject><subject>Mice</subject><subject>Miscellaneous</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Recombinant Proteins</subject><subject>Regulatory factors and their cellular receptors</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9LHTEUxUNR7KvtRxBmUaRdjE0mfyZZiYjaguCiFroL92VuamQmo0lGaD-9M-89Ht25SuD8Tu7NOYScMHrGKFPfflJKVd0Y9vuL1l81pczU6h1ZMWpo3TAqDshqj7wnH3J-nBkmlDkiR40WWjK5Io_3D1g57Puph1SFWDBF6MM_KGGM1eirhG4c1iFCLNUfGAbYQh7TrHfBz5dc-TQOVXmAsjgilClB_z-3MX4khx76jJ925zH5dX11f_m9vr27-XF5cVs70dBSA2dGrXHNBVJpmOhaaIzq0HDuOG-F160BFIpqxaVuWt5q7rlGymVnBDP8mJxu331K4_OEudgh5OWLEHGcsm21FEZL9SbIJFONEHQG5RZ0acw5obdPKQyQ_lpG7VKG3ZRhl6St1nZThl0GnOwGTOsBu71rl_6sf97pkB30PkF0Ie8x1QrON-PPtxjOqb0ETDa7gNFhF-Z2iu3G8MYir1owpoE</recordid><startdate>19881130</startdate><enddate>19881130</enddate><creator>Kushnaryov, Vladimir M.</creator><creator>MacDonald, Hector S.</creator><creator>Sedmak, J. 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Humoral and cellular immunity</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>Cell Compartmentation</topic><topic>Cell Nucleus - metabolism</topic><topic>Coated Pits, Cell-Membrane - metabolism</topic><topic>Cytoplasm - metabolism</topic><topic>Endocytosis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Immunobiology</topic><topic>In Vitro Techniques</topic><topic>Interferon-gamma - pharmacokinetics</topic><topic>L Cells (Cell Line) - metabolism</topic><topic>Mice</topic><topic>Miscellaneous</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Recombinant Proteins</topic><topic>Regulatory factors and their cellular receptors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kushnaryov, Vladimir M.</creatorcontrib><creatorcontrib>MacDonald, Hector S.</creatorcontrib><creatorcontrib>Sedmak, J. 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−10M). Both
125I-labeled MuIFNs are rapidly internalized by cells at 37°C, although recombinant IFN is internalized somewhat more slowly than natural IFN (t
1/2=90 sec and 45 sec, respectively). Immunoelectronmicroscopy showed that the majority of bound recombinant MuIFN-γ was located on the plasma membrane outside of coated areas, whereas natural interferon was found mainly in coated pits. At 37°C most of the recombinant molecules entered the cytoplasm in pinocytotic vesicles, while natural interferon was internalized by the specific mechanism of receptor-mediated endocytosis [1]. However, nearly equal amounts of immunocytochemically detectable molecules of both IFNs were found in the cell nucleus within 2–3 min incubation at 37°C. Thus, the process of translocation of the recombinant IFN-γ appears to differ from that of the natural product.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>2848515</pmid><doi>10.1016/S0006-291X(88)80019-6</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1988-11, Vol.157 (1), p.109-114 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Analysis of the immune response. Humoral and cellular immunity Animals Biological and medical sciences Biological Transport Cell Compartmentation Cell Nucleus - metabolism Coated Pits, Cell-Membrane - metabolism Cytoplasm - metabolism Endocytosis Fundamental and applied biological sciences. Psychology Fundamental immunology Immunobiology In Vitro Techniques Interferon-gamma - pharmacokinetics L Cells (Cell Line) - metabolism Mice Miscellaneous Receptors, Cell Surface - metabolism Recombinant Proteins Regulatory factors and their cellular receptors |
| title | The cellular internalization of recombinant gamma interferon differs from that of natural interferon gamma |
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