Associations of UBE2I with RAD52, UBL1, p53, and RAD51 Proteins in a Yeast Two-Hybrid System
The yeast RAD52-dependent pathway is involved in DNA recombination and double-strand break repair. Yeast ubiquitin-conjugating enzyme UBC9 participates in S- and M-phase cyclin degradation and mitotic control. Using the human RAD52 protein as the “bait” in a yeast two-hybrid system, we have identifi...
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| Veröffentlicht in: | Genomics 1996-10, Vol.37 (2), p.183-186 |
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| container_title | Genomics |
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| creator | Shen, Zhiyuan Pardington-Purtymun, Paige E. Comeaux, Jarmon C. Moyzis, Robert K. Chen, David J. |
| description | The yeast RAD52-dependent pathway is involved in DNA recombination and double-strand break repair. Yeast ubiquitin-conjugating enzyme UBC9 participates in S- and M-phase cyclin degradation and mitotic control. Using the human RAD52 protein as the “bait” in a yeast two-hybrid system, we have identified a human homolog of yeast UBC9, designated UBE2I, that interacts with RAD52, RAD51, p53, and a ubiquitin-like protein UBL1. These interactions are UBE2I-specific, since another DNA repair-related ubiquitin-conjugating enzyme, RAD6 (UBC2), does not interact with these proteins. The interaction of UBE2I with RAD52 is mediated by RAD52’s self-association region. These results suggest that the RAD52-dependent processes, cell cycle control, p53-mediated pathway(s), and ubiquitination interact through human UBE2I. |
| doi_str_mv | 10.1006/geno.1996.0540 |
| format | Article |
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Yeast ubiquitin-conjugating enzyme UBC9 participates in S- and M-phase cyclin degradation and mitotic control. Using the human RAD52 protein as the “bait” in a yeast two-hybrid system, we have identified a human homolog of yeast UBC9, designated UBE2I, that interacts with RAD52, RAD51, p53, and a ubiquitin-like protein UBL1. These interactions are UBE2I-specific, since another DNA repair-related ubiquitin-conjugating enzyme, RAD6 (UBC2), does not interact with these proteins. The interaction of UBE2I with RAD52 is mediated by RAD52’s self-association region. These results suggest that the RAD52-dependent processes, cell cycle control, p53-mediated pathway(s), and ubiquitination interact through human UBE2I.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1006/geno.1996.0540</identifier><identifier>PMID: 8921390</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>ANIMAL CELLS ; BIOLOGY AND MEDICINE, BASIC STUDIES ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; CELL CYCLE ; Chromosome Mapping ; Chromosomes, Human, Pair 16 ; DNA REPAIR ; DNA SEQUENCING ; DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; DNA-CLONING ; ENZYMES ; FLUORESCENCE ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; GENE RECOMBINATION ; GENE REGULATION ; GENETIC MAPPING ; HUMAN CHROMOSOME 16 ; Humans ; HYBRIDIZATION ; IN-SITU HYBRIDIZATION ; Ligases - genetics ; Ligases - metabolism ; Male ; MEIOSIS ; MITOSIS ; Molecular Sequence Data ; Protein Binding ; PROTEINS ; Rad51 Recombinase ; Rad52 DNA Repair and Recombination Protein ; RNA, Messenger - metabolism ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae Proteins ; Schizosaccharomyces pombe Proteins ; STRAND BREAKS ; STRUCTURE-ACTIVITY RELATIONSHIPS ; SUMO-1 Protein ; Testis - metabolism ; Tumor Suppressor Protein p53 - metabolism ; Ubiquitin-Conjugating Enzymes ; YEASTS</subject><ispartof>Genomics, 1996-10, Vol.37 (2), p.183-186</ispartof><rights>1996 Academic Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3200-7f8a1c25502f69d45d06413901435eeeafbf82f74466ec6389a6eb92302e956d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0888754396905406$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,881,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8921390$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/577008$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Shen, Zhiyuan</creatorcontrib><creatorcontrib>Pardington-Purtymun, Paige E.</creatorcontrib><creatorcontrib>Comeaux, Jarmon C.</creatorcontrib><creatorcontrib>Moyzis, Robert K.</creatorcontrib><creatorcontrib>Chen, David J.</creatorcontrib><title>Associations of UBE2I with RAD52, UBL1, p53, and RAD51 Proteins in a Yeast Two-Hybrid System</title><title>Genomics</title><addtitle>Genomics</addtitle><description>The yeast RAD52-dependent pathway is involved in DNA recombination and double-strand break repair. Yeast ubiquitin-conjugating enzyme UBC9 participates in S- and M-phase cyclin degradation and mitotic control. Using the human RAD52 protein as the “bait” in a yeast two-hybrid system, we have identified a human homolog of yeast UBC9, designated UBE2I, that interacts with RAD52, RAD51, p53, and a ubiquitin-like protein UBL1. These interactions are UBE2I-specific, since another DNA repair-related ubiquitin-conjugating enzyme, RAD6 (UBC2), does not interact with these proteins. The interaction of UBE2I with RAD52 is mediated by RAD52’s self-association region. These results suggest that the RAD52-dependent processes, cell cycle control, p53-mediated pathway(s), and ubiquitination interact through human UBE2I.</description><subject>ANIMAL CELLS</subject><subject>BIOLOGY AND MEDICINE, BASIC STUDIES</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>CELL CYCLE</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Human, Pair 16</subject><subject>DNA REPAIR</subject><subject>DNA SEQUENCING</subject><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>DNA-CLONING</subject><subject>ENZYMES</subject><subject>FLUORESCENCE</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>GENE RECOMBINATION</subject><subject>GENE REGULATION</subject><subject>GENETIC MAPPING</subject><subject>HUMAN CHROMOSOME 16</subject><subject>Humans</subject><subject>HYBRIDIZATION</subject><subject>IN-SITU HYBRIDIZATION</subject><subject>Ligases - genetics</subject><subject>Ligases - metabolism</subject><subject>Male</subject><subject>MEIOSIS</subject><subject>MITOSIS</subject><subject>Molecular Sequence Data</subject><subject>Protein Binding</subject><subject>PROTEINS</subject><subject>Rad51 Recombinase</subject><subject>Rad52 DNA Repair and Recombination Protein</subject><subject>RNA, Messenger - metabolism</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>Schizosaccharomyces pombe Proteins</subject><subject>STRAND BREAKS</subject><subject>STRUCTURE-ACTIVITY RELATIONSHIPS</subject><subject>SUMO-1 Protein</subject><subject>Testis - metabolism</subject><subject>Tumor Suppressor Protein p53 - metabolism</subject><subject>Ubiquitin-Conjugating Enzymes</subject><subject>YEASTS</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE2LFDEQhoMo67h69SbEi6fpsfLZyXFc9wsGFN09CELIpKvdyExnTDIu8-_tdgZvngqqnnp5eQh5zWDBAPT7HzikBbNWL0BJeEJmDIxtjJb6KZmBMaZplRTPyYtSfgKAFYafkTNjORMWZuT7spQUoq8xDYWmnt5_uOS39DHWB_pl-VHx-bhZsTndKTGnfuj-bhn9nFPFOL7EgXr6DX2p9O4xNTeHdY4d_XooFbcvybPebwq-Os1zcn91eXdx06w-Xd9eLFdNEBygaXvjWeBKAe-17aTqQMupHpNCIaLv173hfSul1hi0MNZrXFsugKNVuhPn5O0xN5UaXQmxYngIaRgwVKfaFsCMzLsjs8vp1x5LddtYAm42fsC0L641SpqWTeDiCIacSsnYu12OW58PjoGblLtJuZuUu0n5-PDmlLxfb7H7h58cj3dzvOOo4HfEPDXEIWAX81SwS_F_0X8AHTGK0w</recordid><startdate>19961015</startdate><enddate>19961015</enddate><creator>Shen, Zhiyuan</creator><creator>Pardington-Purtymun, Paige E.</creator><creator>Comeaux, Jarmon C.</creator><creator>Moyzis, Robert K.</creator><creator>Chen, David J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>19961015</creationdate><title>Associations of UBE2I with RAD52, UBL1, p53, and RAD51 Proteins in a Yeast Two-Hybrid System</title><author>Shen, Zhiyuan ; Pardington-Purtymun, Paige E. ; Comeaux, Jarmon C. ; Moyzis, Robert K. ; Chen, David J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3200-7f8a1c25502f69d45d06413901435eeeafbf82f74466ec6389a6eb92302e956d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>ANIMAL CELLS</topic><topic>BIOLOGY AND MEDICINE, BASIC STUDIES</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>CELL CYCLE</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Human, Pair 16</topic><topic>DNA REPAIR</topic><topic>DNA SEQUENCING</topic><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>DNA-CLONING</topic><topic>ENZYMES</topic><topic>FLUORESCENCE</topic><topic>Fungal Proteins - genetics</topic><topic>Fungal Proteins - metabolism</topic><topic>GENE RECOMBINATION</topic><topic>GENE REGULATION</topic><topic>GENETIC MAPPING</topic><topic>HUMAN CHROMOSOME 16</topic><topic>Humans</topic><topic>HYBRIDIZATION</topic><topic>IN-SITU HYBRIDIZATION</topic><topic>Ligases - genetics</topic><topic>Ligases - metabolism</topic><topic>Male</topic><topic>MEIOSIS</topic><topic>MITOSIS</topic><topic>Molecular Sequence Data</topic><topic>Protein Binding</topic><topic>PROTEINS</topic><topic>Rad51 Recombinase</topic><topic>Rad52 DNA Repair and Recombination Protein</topic><topic>RNA, Messenger - metabolism</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>Schizosaccharomyces pombe Proteins</topic><topic>STRAND BREAKS</topic><topic>STRUCTURE-ACTIVITY RELATIONSHIPS</topic><topic>SUMO-1 Protein</topic><topic>Testis - metabolism</topic><topic>Tumor Suppressor Protein p53 - metabolism</topic><topic>Ubiquitin-Conjugating Enzymes</topic><topic>YEASTS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shen, Zhiyuan</creatorcontrib><creatorcontrib>Pardington-Purtymun, Paige E.</creatorcontrib><creatorcontrib>Comeaux, Jarmon C.</creatorcontrib><creatorcontrib>Moyzis, Robert K.</creatorcontrib><creatorcontrib>Chen, David J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Genomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shen, Zhiyuan</au><au>Pardington-Purtymun, Paige E.</au><au>Comeaux, Jarmon C.</au><au>Moyzis, Robert K.</au><au>Chen, David J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Associations of UBE2I with RAD52, UBL1, p53, and RAD51 Proteins in a Yeast Two-Hybrid System</atitle><jtitle>Genomics</jtitle><addtitle>Genomics</addtitle><date>1996-10-15</date><risdate>1996</risdate><volume>37</volume><issue>2</issue><spage>183</spage><epage>186</epage><pages>183-186</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>The yeast RAD52-dependent pathway is involved in DNA recombination and double-strand break repair. Yeast ubiquitin-conjugating enzyme UBC9 participates in S- and M-phase cyclin degradation and mitotic control. Using the human RAD52 protein as the “bait” in a yeast two-hybrid system, we have identified a human homolog of yeast UBC9, designated UBE2I, that interacts with RAD52, RAD51, p53, and a ubiquitin-like protein UBL1. These interactions are UBE2I-specific, since another DNA repair-related ubiquitin-conjugating enzyme, RAD6 (UBC2), does not interact with these proteins. The interaction of UBE2I with RAD52 is mediated by RAD52’s self-association region. These results suggest that the RAD52-dependent processes, cell cycle control, p53-mediated pathway(s), and ubiquitination interact through human UBE2I.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>8921390</pmid><doi>10.1006/geno.1996.0540</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | ANIMAL CELLS BIOLOGY AND MEDICINE, BASIC STUDIES Carrier Proteins - genetics Carrier Proteins - metabolism CELL CYCLE Chromosome Mapping Chromosomes, Human, Pair 16 DNA REPAIR DNA SEQUENCING DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism DNA-CLONING ENZYMES FLUORESCENCE Fungal Proteins - genetics Fungal Proteins - metabolism GENE RECOMBINATION GENE REGULATION GENETIC MAPPING HUMAN CHROMOSOME 16 Humans HYBRIDIZATION IN-SITU HYBRIDIZATION Ligases - genetics Ligases - metabolism Male MEIOSIS MITOSIS Molecular Sequence Data Protein Binding PROTEINS Rad51 Recombinase Rad52 DNA Repair and Recombination Protein RNA, Messenger - metabolism Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins STRAND BREAKS STRUCTURE-ACTIVITY RELATIONSHIPS SUMO-1 Protein Testis - metabolism Tumor Suppressor Protein p53 - metabolism Ubiquitin-Conjugating Enzymes YEASTS |
| title | Associations of UBE2I with RAD52, UBL1, p53, and RAD51 Proteins in a Yeast Two-Hybrid System |
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