Cryospectrophotometric Determination of Tumor Intravascular Oxyhemoglobin Saturations: Dependence on Vascular Geometry and Tumor Growth

To delineate the complex relationships between overall tumor oxygenation and vascular configuration, intravascular oxyhemoglobin (HbO2) saturation distributions were measured with cryospectrophotometric techniques. Four factors related to vascular morphometry and tumor growth were evaluated: a) vess...

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Veröffentlicht in:JNCI : Journal of the National Cancer Institute 1988-12, Vol.80 (20), p.1612-1619
Hauptverfasser: Fenton, Bruce M., Rofstad, Einar K., Degner, Frank L., Sutherland, Robert M.
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Rofstad, Einar K.
Degner, Frank L.
Sutherland, Robert M.
description To delineate the complex relationships between overall tumor oxygenation and vascular configuration, intravascular oxyhemoglobin (HbO2) saturation distributions were measured with cryospectrophotometric techniques. Four factors related to vascular morphometry and tumor growth were evaluated: a) vessel diameter, b) distance of vessel from the tumor surface, c) tumor volume, and d) vascular density. To measure intertumor heterogeneity, two murine sarcomas (RIF-1 and KHT) and two human ovarian carcinoma xenografts (OWI and MLS) were utilized. In contrast to skeletal muscle, a preponderance of very low HbO2 saturations was observed for both large and small tumors of all lines. Saturations up to about 90% were also generally present, however, even in very large tumors. Variations in vascular configuration were predominantly tumor-line dependent rather than due to inherent characteristics of the host vasculature, and widely disparate HbO2 distributions were found for alternate lines implanted in identical host mice. Although peripheral saturations remained fairly constant with tumor growth, HbO2 values were markedly lower for vessels nearer the tumor center and further decreased with increasing tumor volume. HbO2 satuations did not change substantially with increasing vascular density (except for KHT tumors), although density did decrease with increasing distance from tumor surface. Combined effects of vessel diameter, tumor volume, and vessel location on HbO2 saturations were complex and varied markedly with both tumor line and vessel class. For specific classes, HbO2 distributions correlated closely with radiobiological hypoxic fractions, i.e., for tumor lines in which hypoxic fraction increased substantially with tumor volume, corresponding HbO2 values decreased, while for lines in which hypoxic fraction remained constant, HbO2 values also were unchanged. Although these trends may also be a function of differing oxygen consumption rates between tumor lines, functional alterations in the rapidly expanding tumor vasculature undoubtedly play a primary role in explaining spatial oxygenation heterogeneities.
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Four factors related to vascular morphometry and tumor growth were evaluated: a) vessel diameter, b) distance of vessel from the tumor surface, c) tumor volume, and d) vascular density. To measure intertumor heterogeneity, two murine sarcomas (RIF-1 and KHT) and two human ovarian carcinoma xenografts (OWI and MLS) were utilized. In contrast to skeletal muscle, a preponderance of very low HbO2 saturations was observed for both large and small tumors of all lines. Saturations up to about 90% were also generally present, however, even in very large tumors. Variations in vascular configuration were predominantly tumor-line dependent rather than due to inherent characteristics of the host vasculature, and widely disparate HbO2 distributions were found for alternate lines implanted in identical host mice. Although peripheral saturations remained fairly constant with tumor growth, HbO2 values were markedly lower for vessels nearer the tumor center and further decreased with increasing tumor volume. HbO2 satuations did not change substantially with increasing vascular density (except for KHT tumors), although density did decrease with increasing distance from tumor surface. Combined effects of vessel diameter, tumor volume, and vessel location on HbO2 saturations were complex and varied markedly with both tumor line and vessel class. For specific classes, HbO2 distributions correlated closely with radiobiological hypoxic fractions, i.e., for tumor lines in which hypoxic fraction increased substantially with tumor volume, corresponding HbO2 values decreased, while for lines in which hypoxic fraction remained constant, HbO2 values also were unchanged. Although these trends may also be a function of differing oxygen consumption rates between tumor lines, functional alterations in the rapidly expanding tumor vasculature undoubtedly play a primary role in explaining spatial oxygenation heterogeneities.</abstract><cop>Cary, NC</cop><pub>Oxford University Press</pub><pmid>3193479</pmid><doi>10.1093/jnci/80.20.1612</doi><tpages>8</tpages></addata></record>
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subjects Animal tumors. Experimental tumors
Animals
Biological and medical sciences
Blood Vessels - metabolism
Blood Vessels - pathology
Experimental tumors, general aspects
Female
Freezing
Humans
Medical sciences
Mice
Mice, Inbred C3H
Neoplasms, Experimental - blood supply
Neoplasms, Experimental - metabolism
Neoplasms, Experimental - pathology
Oxyhemoglobins - metabolism
Spectrophotometry
Tumor Cells, Cultured
Tumors
title Cryospectrophotometric Determination of Tumor Intravascular Oxyhemoglobin Saturations: Dependence on Vascular Geometry and Tumor Growth
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