Cryospectrophotometric Determination of Tumor Intravascular Oxyhemoglobin Saturations: Dependence on Vascular Geometry and Tumor Growth
To delineate the complex relationships between overall tumor oxygenation and vascular configuration, intravascular oxyhemoglobin (HbO2) saturation distributions were measured with cryospectrophotometric techniques. Four factors related to vascular morphometry and tumor growth were evaluated: a) vess...
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description | To delineate the complex relationships between overall tumor oxygenation and vascular configuration, intravascular oxyhemoglobin (HbO2) saturation distributions were measured with cryospectrophotometric techniques. Four factors related to vascular morphometry and tumor growth were evaluated: a) vessel diameter, b) distance of vessel from the tumor surface, c) tumor volume, and d) vascular density. To measure intertumor heterogeneity, two murine sarcomas (RIF-1 and KHT) and two human ovarian carcinoma xenografts (OWI and MLS) were utilized. In contrast to skeletal muscle, a preponderance of very low HbO2 saturations was observed for both large and small tumors of all lines. Saturations up to about 90% were also generally present, however, even in very large tumors. Variations in vascular configuration were predominantly tumor-line dependent rather than due to inherent characteristics of the host vasculature, and widely disparate HbO2 distributions were found for alternate lines implanted in identical host mice. Although peripheral saturations remained fairly constant with tumor growth, HbO2 values were markedly lower for vessels nearer the tumor center and further decreased with increasing tumor volume. HbO2 satuations did not change substantially with increasing vascular density (except for KHT tumors), although density did decrease with increasing distance from tumor surface. Combined effects of vessel diameter, tumor volume, and vessel location on HbO2 saturations were complex and varied markedly with both tumor line and vessel class. For specific classes, HbO2 distributions correlated closely with radiobiological hypoxic fractions, i.e., for tumor lines in which hypoxic fraction increased substantially with tumor volume, corresponding HbO2 values decreased, while for lines in which hypoxic fraction remained constant, HbO2 values also were unchanged. Although these trends may also be a function of differing oxygen consumption rates between tumor lines, functional alterations in the rapidly expanding tumor vasculature undoubtedly play a primary role in explaining spatial oxygenation heterogeneities. |
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Four factors related to vascular morphometry and tumor growth were evaluated: a) vessel diameter, b) distance of vessel from the tumor surface, c) tumor volume, and d) vascular density. To measure intertumor heterogeneity, two murine sarcomas (RIF-1 and KHT) and two human ovarian carcinoma xenografts (OWI and MLS) were utilized. In contrast to skeletal muscle, a preponderance of very low HbO2 saturations was observed for both large and small tumors of all lines. Saturations up to about 90% were also generally present, however, even in very large tumors. Variations in vascular configuration were predominantly tumor-line dependent rather than due to inherent characteristics of the host vasculature, and widely disparate HbO2 distributions were found for alternate lines implanted in identical host mice. Although peripheral saturations remained fairly constant with tumor growth, HbO2 values were markedly lower for vessels nearer the tumor center and further decreased with increasing tumor volume. HbO2 satuations did not change substantially with increasing vascular density (except for KHT tumors), although density did decrease with increasing distance from tumor surface. Combined effects of vessel diameter, tumor volume, and vessel location on HbO2 saturations were complex and varied markedly with both tumor line and vessel class. For specific classes, HbO2 distributions correlated closely with radiobiological hypoxic fractions, i.e., for tumor lines in which hypoxic fraction increased substantially with tumor volume, corresponding HbO2 values decreased, while for lines in which hypoxic fraction remained constant, HbO2 values also were unchanged. Although these trends may also be a function of differing oxygen consumption rates between tumor lines, functional alterations in the rapidly expanding tumor vasculature undoubtedly play a primary role in explaining spatial oxygenation heterogeneities.</description><identifier>ISSN: 0027-8874</identifier><identifier>EISSN: 1460-2105</identifier><identifier>DOI: 10.1093/jnci/80.20.1612</identifier><identifier>PMID: 3193479</identifier><language>eng</language><publisher>Cary, NC: Oxford University Press</publisher><subject>Animal tumors. Experimental tumors ; Animals ; Biological and medical sciences ; Blood Vessels - metabolism ; Blood Vessels - pathology ; Experimental tumors, general aspects ; Female ; Freezing ; Humans ; Medical sciences ; Mice ; Mice, Inbred C3H ; Neoplasms, Experimental - blood supply ; Neoplasms, Experimental - metabolism ; Neoplasms, Experimental - pathology ; Oxyhemoglobins - metabolism ; Spectrophotometry ; Tumor Cells, Cultured ; Tumors</subject><ispartof>JNCI : Journal of the National Cancer Institute, 1988-12, Vol.80 (20), p.1612-1619</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-2b4e3b33a54029beca90061492df5d8c490ddc2b5141dfcd71e09e3ac08bd3923</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7084782$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3193479$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fenton, Bruce M.</creatorcontrib><creatorcontrib>Rofstad, Einar K.</creatorcontrib><creatorcontrib>Degner, Frank L.</creatorcontrib><creatorcontrib>Sutherland, Robert M.</creatorcontrib><title>Cryospectrophotometric Determination of Tumor Intravascular Oxyhemoglobin Saturations: Dependence on Vascular Geometry and Tumor Growth</title><title>JNCI : Journal of the National Cancer Institute</title><addtitle>J Natl Cancer Inst</addtitle><description>To delineate the complex relationships between overall tumor oxygenation and vascular configuration, intravascular oxyhemoglobin (HbO2) saturation distributions were measured with cryospectrophotometric techniques. Four factors related to vascular morphometry and tumor growth were evaluated: a) vessel diameter, b) distance of vessel from the tumor surface, c) tumor volume, and d) vascular density. To measure intertumor heterogeneity, two murine sarcomas (RIF-1 and KHT) and two human ovarian carcinoma xenografts (OWI and MLS) were utilized. In contrast to skeletal muscle, a preponderance of very low HbO2 saturations was observed for both large and small tumors of all lines. Saturations up to about 90% were also generally present, however, even in very large tumors. Variations in vascular configuration were predominantly tumor-line dependent rather than due to inherent characteristics of the host vasculature, and widely disparate HbO2 distributions were found for alternate lines implanted in identical host mice. Although peripheral saturations remained fairly constant with tumor growth, HbO2 values were markedly lower for vessels nearer the tumor center and further decreased with increasing tumor volume. HbO2 satuations did not change substantially with increasing vascular density (except for KHT tumors), although density did decrease with increasing distance from tumor surface. Combined effects of vessel diameter, tumor volume, and vessel location on HbO2 saturations were complex and varied markedly with both tumor line and vessel class. For specific classes, HbO2 distributions correlated closely with radiobiological hypoxic fractions, i.e., for tumor lines in which hypoxic fraction increased substantially with tumor volume, corresponding HbO2 values decreased, while for lines in which hypoxic fraction remained constant, HbO2 values also were unchanged. Although these trends may also be a function of differing oxygen consumption rates between tumor lines, functional alterations in the rapidly expanding tumor vasculature undoubtedly play a primary role in explaining spatial oxygenation heterogeneities.</description><subject>Animal tumors. Experimental tumors</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood Vessels - metabolism</subject><subject>Blood Vessels - pathology</subject><subject>Experimental tumors, general aspects</subject><subject>Female</subject><subject>Freezing</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Neoplasms, Experimental - blood supply</subject><subject>Neoplasms, Experimental - metabolism</subject><subject>Neoplasms, Experimental - pathology</subject><subject>Oxyhemoglobins - metabolism</subject><subject>Spectrophotometry</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors</subject><issn>0027-8874</issn><issn>1460-2105</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE9v1DAQxS0EKkvhzAkpB8QtXf9NYm5oC9uilYrEglAvlmNP2JQkTm0Hup-Ar423G3Yuo9H7vTfSQ-g1wRcES7a8G0y7rPAFTXdB6BO0ILzAOSVYPEULjGmZV1XJn6MXIdzhNJLyM3TGiGS8lAv0d-X3LoxgonfjzkXXQ_StyS4hgu_bQcfWDZlrsu3UO59dD9Hr3zqYqdM-u3nY76B3PztXt0P2VcfJP_LhffKPMFgYDGTJ__2_Yw2PD_aZHuwcufbuT9y9RM8a3QV4Ne9z9O3Tx-3qKt_crK9XHza5YULGnNYcWM2YFhxTWYPREuOCcEltI2xluMTWGloLwoltjC0JYAlMG1zVlknKztG7Y-7o3f0EIaq-DQa6Tg_gpqDKSnAhRJHA5RE03oXgoVGjb3vt94pgdaheHapXFVY03an65HgzR091D_bEz10n_e2spzZ013idAsIJK3HFy-oQkx-xNkR4OMna_1JFyUqhrn7cKiwu6ebz7Re1Zf8A8aKf1A</recordid><startdate>19881221</startdate><enddate>19881221</enddate><creator>Fenton, Bruce M.</creator><creator>Rofstad, Einar K.</creator><creator>Degner, Frank L.</creator><creator>Sutherland, Robert M.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19881221</creationdate><title>Cryospectrophotometric Determination of Tumor Intravascular Oxyhemoglobin Saturations: Dependence on Vascular Geometry and Tumor Growth</title><author>Fenton, Bruce M. ; Rofstad, Einar K. ; Degner, Frank L. ; Sutherland, Robert M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-2b4e3b33a54029beca90061492df5d8c490ddc2b5141dfcd71e09e3ac08bd3923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animal tumors. Experimental tumors</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood Vessels - metabolism</topic><topic>Blood Vessels - pathology</topic><topic>Experimental tumors, general aspects</topic><topic>Female</topic><topic>Freezing</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Neoplasms, Experimental - blood supply</topic><topic>Neoplasms, Experimental - metabolism</topic><topic>Neoplasms, Experimental - pathology</topic><topic>Oxyhemoglobins - metabolism</topic><topic>Spectrophotometry</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fenton, Bruce M.</creatorcontrib><creatorcontrib>Rofstad, Einar K.</creatorcontrib><creatorcontrib>Degner, Frank L.</creatorcontrib><creatorcontrib>Sutherland, Robert M.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>JNCI : Journal of the National Cancer Institute</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fenton, Bruce M.</au><au>Rofstad, Einar K.</au><au>Degner, Frank L.</au><au>Sutherland, Robert M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryospectrophotometric Determination of Tumor Intravascular Oxyhemoglobin Saturations: Dependence on Vascular Geometry and Tumor Growth</atitle><jtitle>JNCI : Journal of the National Cancer Institute</jtitle><addtitle>J Natl Cancer Inst</addtitle><date>1988-12-21</date><risdate>1988</risdate><volume>80</volume><issue>20</issue><spage>1612</spage><epage>1619</epage><pages>1612-1619</pages><issn>0027-8874</issn><eissn>1460-2105</eissn><abstract>To delineate the complex relationships between overall tumor oxygenation and vascular configuration, intravascular oxyhemoglobin (HbO2) saturation distributions were measured with cryospectrophotometric techniques. Four factors related to vascular morphometry and tumor growth were evaluated: a) vessel diameter, b) distance of vessel from the tumor surface, c) tumor volume, and d) vascular density. To measure intertumor heterogeneity, two murine sarcomas (RIF-1 and KHT) and two human ovarian carcinoma xenografts (OWI and MLS) were utilized. In contrast to skeletal muscle, a preponderance of very low HbO2 saturations was observed for both large and small tumors of all lines. Saturations up to about 90% were also generally present, however, even in very large tumors. Variations in vascular configuration were predominantly tumor-line dependent rather than due to inherent characteristics of the host vasculature, and widely disparate HbO2 distributions were found for alternate lines implanted in identical host mice. Although peripheral saturations remained fairly constant with tumor growth, HbO2 values were markedly lower for vessels nearer the tumor center and further decreased with increasing tumor volume. HbO2 satuations did not change substantially with increasing vascular density (except for KHT tumors), although density did decrease with increasing distance from tumor surface. Combined effects of vessel diameter, tumor volume, and vessel location on HbO2 saturations were complex and varied markedly with both tumor line and vessel class. For specific classes, HbO2 distributions correlated closely with radiobiological hypoxic fractions, i.e., for tumor lines in which hypoxic fraction increased substantially with tumor volume, corresponding HbO2 values decreased, while for lines in which hypoxic fraction remained constant, HbO2 values also were unchanged. Although these trends may also be a function of differing oxygen consumption rates between tumor lines, functional alterations in the rapidly expanding tumor vasculature undoubtedly play a primary role in explaining spatial oxygenation heterogeneities.</abstract><cop>Cary, NC</cop><pub>Oxford University Press</pub><pmid>3193479</pmid><doi>10.1093/jnci/80.20.1612</doi><tpages>8</tpages></addata></record> |
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subjects | Animal tumors. Experimental tumors Animals Biological and medical sciences Blood Vessels - metabolism Blood Vessels - pathology Experimental tumors, general aspects Female Freezing Humans Medical sciences Mice Mice, Inbred C3H Neoplasms, Experimental - blood supply Neoplasms, Experimental - metabolism Neoplasms, Experimental - pathology Oxyhemoglobins - metabolism Spectrophotometry Tumor Cells, Cultured Tumors |
title | Cryospectrophotometric Determination of Tumor Intravascular Oxyhemoglobin Saturations: Dependence on Vascular Geometry and Tumor Growth |
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