An improved technique for the assay of red blood cell superoxide dismutase (SOD) activity
Superoxide dismutase (SOD) activity of erythrocytes from normal pregnant women (controls) and from pre-eclamptic toxaemia (PET) cases was determined by the method of Winterbourn et al. (J Lab Clin Med 1975;85:337–341) with slight modifications. Instead of the Michaelis-Menten type of plot that is us...
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| Veröffentlicht in: | Clinica chimica acta 1996-03, Vol.247 (1), p.1-6 |
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| container_title | Clinica chimica acta |
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| creator | Ilouno, L.E Shu, E.N Igbokwe, G.E |
| description | Superoxide dismutase (SOD) activity of erythrocytes from normal pregnant women (controls) and from pre-eclamptic toxaemia (PET) cases was determined by the method of Winterbourn et al. (J Lab Clin Med 1975;85:337–341) with slight modifications. Instead of the Michaelis-Menten type of plot that is usually obtained and which these authors have found to be imprecise, a linear plot was obtained when the reciprocal values for the percentage inhibition of nitroblue tetrazolium (NBT) reduction were plotted against the corresponding volumes of red blood cell (RBC) extract. This plot was found to give precise values for the volumes of RBC extracts required for 50% inhibition of NBT reduction. Consequently, a clearer understanding is beginning to emerge regarding the SOD activity of PET cases compared with that of normal pregnant women. |
| doi_str_mv | 10.1016/0009-8981(95)06193-2 |
| format | Article |
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(J Lab Clin Med 1975;85:337–341) with slight modifications. Instead of the Michaelis-Menten type of plot that is usually obtained and which these authors have found to be imprecise, a linear plot was obtained when the reciprocal values for the percentage inhibition of nitroblue tetrazolium (NBT) reduction were plotted against the corresponding volumes of red blood cell (RBC) extract. This plot was found to give precise values for the volumes of RBC extracts required for 50% inhibition of NBT reduction. 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(J Lab Clin Med 1975;85:337–341) with slight modifications. Instead of the Michaelis-Menten type of plot that is usually obtained and which these authors have found to be imprecise, a linear plot was obtained when the reciprocal values for the percentage inhibition of nitroblue tetrazolium (NBT) reduction were plotted against the corresponding volumes of red blood cell (RBC) extract. This plot was found to give precise values for the volumes of RBC extracts required for 50% inhibition of NBT reduction. Consequently, a clearer understanding is beginning to emerge regarding the SOD activity of PET cases compared with that of normal pregnant women.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Blood Chemical Analysis - methods</subject><subject>Diseases of mother, fetus and pregnancy</subject><subject>Enzyme Activation - drug effects</subject><subject>Erythrocytes - enzymology</subject><subject>Female</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Nitroblue tetrazolium</subject><subject>Nitroblue Tetrazolium - pharmacology</subject><subject>Oxidation-Reduction</subject><subject>Pre-Eclampsia - enzymology</subject><subject>Pre-eclamptic toxaemia</subject><subject>Pregnancy</subject><subject>Pregnancy. Fetus. Placenta</subject><subject>Red blood cells</subject><subject>Superoxide dismutase</subject><subject>Superoxide Dismutase - blood</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLHTEYhoO02FPrP7CQRSm6GM1tLtkUxN4EwYXtoquQyxeMzEyOyczB8-_NeA5n2VVI3uf7ePMgdEbJJSW0uSKEyKqTHT2X9QVpqOQVO0Ir2rW84kKyd2h1QD6gjzk_laso4DE67iQjjLEV-nc94jCsU9yAwxPYxzE8z4B9THh6BKxz1lscPU4lNn2MDlvoe5znNaT4EhxgF_IwTzoDPn-4_36BtZ3CJkzbT-i9132G0_15gv7-_PHn5nd1d__r9ub6rrK8a6bKCcZq5yj3hgnCHIPWWaMNF4yamjZWksYaoSlpXevKq6m9Z6TmtDPeEcdP0Nfd3vKJUj1Pagh5KalHiHNWbVeLmklWQLEDbYo5J_BqncKg01ZRohajatGlFl1K1urNqFrGPu_3z2YAdxjaKyz5l32us9W9T3q0IR8wXhaLlhTs2w6D4mITIKlsA4wWXEhgJ-Vi-H-PV14ikXo</recordid><startdate>19960329</startdate><enddate>19960329</enddate><creator>Ilouno, L.E</creator><creator>Shu, E.N</creator><creator>Igbokwe, G.E</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960329</creationdate><title>An improved technique for the assay of red blood cell superoxide dismutase (SOD) activity</title><author>Ilouno, L.E ; Shu, E.N ; Igbokwe, G.E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-d4225dd13fb2402d2e7dcbab3421b516c906cb4a107d7d342b5ff205318bfd0d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Blood Chemical Analysis - methods</topic><topic>Diseases of mother, fetus and pregnancy</topic><topic>Enzyme Activation - drug effects</topic><topic>Erythrocytes - enzymology</topic><topic>Female</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Nitroblue tetrazolium</topic><topic>Nitroblue Tetrazolium - pharmacology</topic><topic>Oxidation-Reduction</topic><topic>Pre-Eclampsia - enzymology</topic><topic>Pre-eclamptic toxaemia</topic><topic>Pregnancy</topic><topic>Pregnancy. Fetus. Placenta</topic><topic>Red blood cells</topic><topic>Superoxide dismutase</topic><topic>Superoxide Dismutase - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ilouno, L.E</creatorcontrib><creatorcontrib>Shu, E.N</creatorcontrib><creatorcontrib>Igbokwe, G.E</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ilouno, L.E</au><au>Shu, E.N</au><au>Igbokwe, G.E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An improved technique for the assay of red blood cell superoxide dismutase (SOD) activity</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1996-03-29</date><risdate>1996</risdate><volume>247</volume><issue>1</issue><spage>1</spage><epage>6</epage><pages>1-6</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><coden>CCATAR</coden><abstract>Superoxide dismutase (SOD) activity of erythrocytes from normal pregnant women (controls) and from pre-eclamptic toxaemia (PET) cases was determined by the method of Winterbourn et al. (J Lab Clin Med 1975;85:337–341) with slight modifications. Instead of the Michaelis-Menten type of plot that is usually obtained and which these authors have found to be imprecise, a linear plot was obtained when the reciprocal values for the percentage inhibition of nitroblue tetrazolium (NBT) reduction were plotted against the corresponding volumes of red blood cell (RBC) extract. This plot was found to give precise values for the volumes of RBC extracts required for 50% inhibition of NBT reduction. Consequently, a clearer understanding is beginning to emerge regarding the SOD activity of PET cases compared with that of normal pregnant women.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>8920222</pmid><doi>10.1016/0009-8981(95)06193-2</doi><tpages>6</tpages></addata></record> |
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| ispartof | Clinica chimica acta, 1996-03, Vol.247 (1), p.1-6 |
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| subjects | Adolescent Adult Biological and medical sciences Blood Chemical Analysis - methods Diseases of mother, fetus and pregnancy Enzyme Activation - drug effects Erythrocytes - enzymology Female Gynecology. Andrology. Obstetrics Humans Medical sciences Nitroblue tetrazolium Nitroblue Tetrazolium - pharmacology Oxidation-Reduction Pre-Eclampsia - enzymology Pre-eclamptic toxaemia Pregnancy Pregnancy. Fetus. Placenta Red blood cells Superoxide dismutase Superoxide Dismutase - blood |
| title | An improved technique for the assay of red blood cell superoxide dismutase (SOD) activity |
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