Failure of a recombinant Babesia bovis antigen to protect cattle against heterologous strain challenge
Groups of cattle were inoculated subcutaneously with (i) a recombinant DNA-derived Babesia bovis protein (KaBbl-GZ) fused to β-galactosidase and combined with adjuvants, or (ii) native β-galactosidase (GZ) plus adjuvant, or (iii) adjuvant only or (iv) a live, attenuated B bovis vaccine. KaBbl-GZ was...
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| Veröffentlicht in: | Research in veterinary science 1988-09, Vol.45 (2), p.267-269 |
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| creator | TIMMS, P. BARRY, D.N. GILL, A.C. SHARP, P.J. DE VOS, A.J. |
| description | Groups of cattle were inoculated subcutaneously with (i) a recombinant DNA-derived Babesia bovis protein (KaBbl-GZ) fused to β-galactosidase and combined with adjuvants, or (ii) native β-galactosidase (GZ) plus adjuvant, or (iii) adjuvant only or (iv) a live, attenuated B bovis vaccine. KaBbl-GZ was produced in the λ gt11-amp3 system as a 5-10 kD babesial polypeptide linked to GZ. KaBbl has previously been shown to be an immunodominant antigen of B bovis, localised at the apex of the parasite, and present in a range of B bovis strains. High levels of GZ antibodies were observed in KaBbl-GZ and GZ inoculated cattle, but specific KaBbl antibodies could not be detected by klisa. Five months after primary inoculation, all cattle were blood challenged with a virulent heterologous B bovis strain. Despite four inoculations with KaBbl-GZ, significant protection against the challenge was not observed. |
| doi_str_mv | 10.1016/S0034-5288(18)30947-0 |
| format | Article |
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KaBbl-GZ was produced in the λ gt11-amp3 system as a 5-10 kD babesial polypeptide linked to GZ. KaBbl has previously been shown to be an immunodominant antigen of B bovis, localised at the apex of the parasite, and present in a range of B bovis strains. High levels of GZ antibodies were observed in KaBbl-GZ and GZ inoculated cattle, but specific KaBbl antibodies could not be detected by klisa. Five months after primary inoculation, all cattle were blood challenged with a virulent heterologous B bovis strain. Despite four inoculations with KaBbl-GZ, significant protection against the challenge was not observed.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/S0034-5288(18)30947-0</identifier><identifier>PMID: 3194602</identifier><language>eng</language><publisher>Amsterdam: Elsevier Ltd</publisher><subject>Adjuvants ; Animals ; Antibodies ; Antigens ; Antigens, Protozoan - administration & dosage ; Babesia - immunology ; Babesia bovis ; Babesiosis - prevention & control ; Biological and medical sciences ; Bovidae ; Cattle ; Cattle Diseases - parasitology ; Cattle Diseases - prevention & control ; Deoxyribonucleic acid ; DNA ; Fundamental and applied biological sciences. Psychology ; Galactosidase ; Injections, Subcutaneous ; Inoculation ; Proteins ; Protozoa ; Recombinant DNA ; Recombinant Proteins ; Veterinary medicine ; β-Galactosidase</subject><ispartof>Research in veterinary science, 1988-09, Vol.45 (2), p.267-269</ispartof><rights>1988</rights><rights>1991 INIST-CNRS</rights><rights>Copyright Elsevier Limited Sep 1988</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c449t-918f2620c1a166e11bb6ab794b8c3d593f01cbffdd12116d60e8979af1aa5c023</citedby><cites>FETCH-LOGICAL-c449t-918f2620c1a166e11bb6ab794b8c3d593f01cbffdd12116d60e8979af1aa5c023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0034-5288(18)30947-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19551013$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3194602$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TIMMS, P.</creatorcontrib><creatorcontrib>BARRY, D.N.</creatorcontrib><creatorcontrib>GILL, A.C.</creatorcontrib><creatorcontrib>SHARP, P.J.</creatorcontrib><creatorcontrib>DE VOS, A.J.</creatorcontrib><title>Failure of a recombinant Babesia bovis antigen to protect cattle against heterologous strain challenge</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>Groups of cattle were inoculated subcutaneously with (i) a recombinant DNA-derived Babesia bovis protein (KaBbl-GZ) fused to β-galactosidase and combined with adjuvants, or (ii) native β-galactosidase (GZ) plus adjuvant, or (iii) adjuvant only or (iv) a live, attenuated B bovis vaccine. KaBbl-GZ was produced in the λ gt11-amp3 system as a 5-10 kD babesial polypeptide linked to GZ. KaBbl has previously been shown to be an immunodominant antigen of B bovis, localised at the apex of the parasite, and present in a range of B bovis strains. High levels of GZ antibodies were observed in KaBbl-GZ and GZ inoculated cattle, but specific KaBbl antibodies could not be detected by klisa. Five months after primary inoculation, all cattle were blood challenged with a virulent heterologous B bovis strain. Despite four inoculations with KaBbl-GZ, significant protection against the challenge was not observed.</description><subject>Adjuvants</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antigens</subject><subject>Antigens, Protozoan - administration & dosage</subject><subject>Babesia - immunology</subject><subject>Babesia bovis</subject><subject>Babesiosis - prevention & control</subject><subject>Biological and medical sciences</subject><subject>Bovidae</subject><subject>Cattle</subject><subject>Cattle Diseases - parasitology</subject><subject>Cattle Diseases - prevention & control</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Galactosidase</subject><subject>Injections, Subcutaneous</subject><subject>Inoculation</subject><subject>Proteins</subject><subject>Protozoa</subject><subject>Recombinant DNA</subject><subject>Recombinant Proteins</subject><subject>Veterinary medicine</subject><subject>β-Galactosidase</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVFrFTEQhRdR6rX6EwoBUfRhNZNNstkn0WJVKPigPofZ7OQ2Ze-mJtmC_97c3ksFX_oUmDlzcma-pjkD_g446Pc_OO9kq4Qxb8C87fgg-5Y_ajagOtEKreFxs7mXPG2e5XzNOZcA_Ulz0sEgNRebxl9gmNdELHqGLJGLuzEsuBT2CUfKAdkYb0NmtRK2tLAS2U2KhVxhDkuZieEWw5ILu6JCKc5xG9fMckm1ytwVzjMtW3rePPE4Z3pxfE-bXxeff55_bS-_f_l2_vGydVIOpR3AeKEFd4CgNQGMo8axH-RoXDepofMc3Oj9NIEA0JPmZIZ-QA-IynHRnTavD7415O-VcrG7kB3NMy5Uc9neKCkV7x4UghLSGDBV-PI_4XVc01KXsIKrevVOib2dOqhcijkn8vYmhR2mPxa43eOyd7jsnoUFY-9wWV7nzo7u67ij6X7qyKf2Xx37mB3OPuHiQv5nPihV3ff_fzjoqB73NlCy2QVaHE2hMi12iuGBJH8BsJexUQ</recordid><startdate>19880901</startdate><enddate>19880901</enddate><creator>TIMMS, P.</creator><creator>BARRY, D.N.</creator><creator>GILL, A.C.</creator><creator>SHARP, P.J.</creator><creator>DE VOS, A.J.</creator><general>Elsevier Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19880901</creationdate><title>Failure of a recombinant Babesia bovis antigen to protect cattle against heterologous strain challenge</title><author>TIMMS, P. ; BARRY, D.N. ; GILL, A.C. ; SHARP, P.J. ; DE VOS, A.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449t-918f2620c1a166e11bb6ab794b8c3d593f01cbffdd12116d60e8979af1aa5c023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Adjuvants</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antigens</topic><topic>Antigens, Protozoan - administration & dosage</topic><topic>Babesia - immunology</topic><topic>Babesia bovis</topic><topic>Babesiosis - prevention & control</topic><topic>Biological and medical sciences</topic><topic>Bovidae</topic><topic>Cattle</topic><topic>Cattle Diseases - parasitology</topic><topic>Cattle Diseases - prevention & control</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Fundamental and applied biological sciences. 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KaBbl-GZ was produced in the λ gt11-amp3 system as a 5-10 kD babesial polypeptide linked to GZ. KaBbl has previously been shown to be an immunodominant antigen of B bovis, localised at the apex of the parasite, and present in a range of B bovis strains. High levels of GZ antibodies were observed in KaBbl-GZ and GZ inoculated cattle, but specific KaBbl antibodies could not be detected by klisa. Five months after primary inoculation, all cattle were blood challenged with a virulent heterologous B bovis strain. Despite four inoculations with KaBbl-GZ, significant protection against the challenge was not observed.</abstract><cop>Amsterdam</cop><pub>Elsevier Ltd</pub><pmid>3194602</pmid><doi>10.1016/S0034-5288(18)30947-0</doi><tpages>3</tpages></addata></record> |
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| ispartof | Research in veterinary science, 1988-09, Vol.45 (2), p.267-269 |
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| subjects | Adjuvants Animals Antibodies Antigens Antigens, Protozoan - administration & dosage Babesia - immunology Babesia bovis Babesiosis - prevention & control Biological and medical sciences Bovidae Cattle Cattle Diseases - parasitology Cattle Diseases - prevention & control Deoxyribonucleic acid DNA Fundamental and applied biological sciences. Psychology Galactosidase Injections, Subcutaneous Inoculation Proteins Protozoa Recombinant DNA Recombinant Proteins Veterinary medicine β-Galactosidase |
| title | Failure of a recombinant Babesia bovis antigen to protect cattle against heterologous strain challenge |
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