Kinetic Analysis of the Cleavage of Natural and Synthetic Substrates by the Serratia Nuclease

The extracellular nuclease from Serratia marcescens is a non‐specific endonuclease that hydrolyzes double‐stranded and single‐stranded DNA and RNA with high specific activity. Steady‐state and pre‐steady‐state kinetic cleavage experiments were performed with natural and synthetic DNA and RNA substra...

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Veröffentlicht in:	European journal of biochemistry 1996-10, Vol.241 (2), p.572-580
Hauptverfasser:	Friedhoff, Peter, Meiss, Gregor, Kolmes, Bettina, Pieper, Uwe, Gimadutdinow, Oleg, Urbanke, Claus, Pingoud, Alfred
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence deoxyribonuclease DNA Endonucleases - metabolism Kinetics modified oligonucleotide Molecular Weight Oligodeoxyribonucleotides - chemical synthesis Oligodeoxyribonucleotides - chemistry Poly dA-dT Polydeoxyribonucleotides pre‐steady‐state kinetics ribonuclease RNA Serratia marcescens Serratia marcescens - enzymology steady‐state kinetics Substrate Specificity
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