Eliminating Amino Acid and Peptide Interference in High-Performance Anion-Exchange Pulsed Amperometric Detection Glycoprotein Monosaccharide Analysis

The monosaccharide content of a glycoprotein is often determined by acid hydrolysis at elevated temperature and subsequent high pH chromatography of the released, underivatized monosaccharides on pellicular anion-exchange resin (HPAE) using pulsed amperometric detection (PAD). We have found that for...

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Veröffentlicht in:	Analytical biochemistry 1996-10, Vol.241 (1), p.128-134
Hauptverfasser:	Weitzhandler, Michael, Pohl, Christopher, Rohrer, Jeffrey, Narayanan, Latha, Slingsby, Rosanne, Avdalovic, Nebojsa
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acids Animals Chromatography, Ion Exchange - methods Glycoproteins - analysis Humans Monosaccharides - analysis Peptides Sensitivity and Specificity
Online-Zugang:	Volltext
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