Trabecular meshwork cells grown on filters. Conductivity and cytochalasin effects
A system was developed to measure the hydraulic conductivity of cultured monolayers of human trabecular meshwork (HTM) cells. By optimizing the cell growth conditions and evaluating a number of filter supports, confluent HTM cells in single layers were obtained for measurement of hydraulic conductiv...
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| Veröffentlicht in: | Investigative ophthalmology & visual science 1988-12, Vol.29 (12), p.1836-1846 |
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| container_issue | 12 |
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| container_title | Investigative ophthalmology & visual science |
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| creator | Perkins, TW Alvarado, JA Polansky, JR Stilwell, L Maglio, M Juster, R |
| description | A system was developed to measure the hydraulic conductivity of cultured monolayers of human trabecular meshwork (HTM) cells. By optimizing the cell growth conditions and evaluating a number of filter supports, confluent HTM cells in single layers were obtained for measurement of hydraulic conductivity. The HTM monolayers had hydraulic conductivities of 0.3-2.0 microliters/min/mm Hg/cm2 measured at near-physiological flow rates. Evaluations of cytochalasin B (CB) effects on the hydraulic conductivity of our HTM monolayers revealed that CB (10(-6) to 10(-5) M) caused a dramatic dose-related increase in conductivity within 10 to 30 min, which parallels CB effects on outflow facility in vivo. Morphologic observations show that the increase in hydraulic conductivity was accompanied by a retraction of the trabecular cells and widening of the intercellular spaces. Our findings suggest that growth of HTM cells on filter supports can provide a useful in vitro system to study the regulation of aqueous outflow. |
| format | Article |
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Conductivity and cytochalasin effects</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Perkins, TW ; Alvarado, JA ; Polansky, JR ; Stilwell, L ; Maglio, M ; Juster, R</creator><creatorcontrib>Perkins, TW ; Alvarado, JA ; Polansky, JR ; Stilwell, L ; Maglio, M ; Juster, R</creatorcontrib><description>A system was developed to measure the hydraulic conductivity of cultured monolayers of human trabecular meshwork (HTM) cells. By optimizing the cell growth conditions and evaluating a number of filter supports, confluent HTM cells in single layers were obtained for measurement of hydraulic conductivity. The HTM monolayers had hydraulic conductivities of 0.3-2.0 microliters/min/mm Hg/cm2 measured at near-physiological flow rates. Evaluations of cytochalasin B (CB) effects on the hydraulic conductivity of our HTM monolayers revealed that CB (10(-6) to 10(-5) M) caused a dramatic dose-related increase in conductivity within 10 to 30 min, which parallels CB effects on outflow facility in vivo. Morphologic observations show that the increase in hydraulic conductivity was accompanied by a retraction of the trabecular cells and widening of the intercellular spaces. Our findings suggest that growth of HTM cells on filter supports can provide a useful in vitro system to study the regulation of aqueous outflow.</description><identifier>ISSN: 0146-0404</identifier><identifier>EISSN: 1552-5783</identifier><identifier>PMID: 3192373</identifier><identifier>CODEN: IOVSDA</identifier><language>eng</language><publisher>Rockville, MD: ARVO</publisher><subject>Adult ; Biological and medical sciences ; Biological Transport - drug effects ; Body Fluids - drug effects ; Body Fluids - metabolism ; Cell Division ; Cells, Cultured ; Cytochalasin B - pharmacology ; Eye and associated structures. Visual pathways and centers. Vision ; Filtration - instrumentation ; Fundamental and applied biological sciences. Psychology ; Humans ; Methods ; Perfusion ; Permeability ; Trabecular Meshwork - cytology ; Trabecular Meshwork - drug effects ; Trabecular Meshwork - physiology ; Vertebrates: nervous system and sense organs</subject><ispartof>Investigative ophthalmology & visual science, 1988-12, Vol.29 (12), p.1836-1846</ispartof><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7070795$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3192373$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Perkins, TW</creatorcontrib><creatorcontrib>Alvarado, JA</creatorcontrib><creatorcontrib>Polansky, JR</creatorcontrib><creatorcontrib>Stilwell, L</creatorcontrib><creatorcontrib>Maglio, M</creatorcontrib><creatorcontrib>Juster, R</creatorcontrib><title>Trabecular meshwork cells grown on filters. Conductivity and cytochalasin effects</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>A system was developed to measure the hydraulic conductivity of cultured monolayers of human trabecular meshwork (HTM) cells. By optimizing the cell growth conditions and evaluating a number of filter supports, confluent HTM cells in single layers were obtained for measurement of hydraulic conductivity. The HTM monolayers had hydraulic conductivities of 0.3-2.0 microliters/min/mm Hg/cm2 measured at near-physiological flow rates. Evaluations of cytochalasin B (CB) effects on the hydraulic conductivity of our HTM monolayers revealed that CB (10(-6) to 10(-5) M) caused a dramatic dose-related increase in conductivity within 10 to 30 min, which parallels CB effects on outflow facility in vivo. Morphologic observations show that the increase in hydraulic conductivity was accompanied by a retraction of the trabecular cells and widening of the intercellular spaces. Our findings suggest that growth of HTM cells on filter supports can provide a useful in vitro system to study the regulation of aqueous outflow.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Biological Transport - drug effects</subject><subject>Body Fluids - drug effects</subject><subject>Body Fluids - metabolism</subject><subject>Cell Division</subject><subject>Cells, Cultured</subject><subject>Cytochalasin B - pharmacology</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Filtration - instrumentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Methods</subject><subject>Perfusion</subject><subject>Permeability</subject><subject>Trabecular Meshwork - cytology</subject><subject>Trabecular Meshwork - drug effects</subject><subject>Trabecular Meshwork - physiology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0146-0404</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kEtLxDAUhYMo4zj6E4QsRFeVPJqmXcrgCwZEGNfhJk2n0bQdk9Yy_97KFDmLuzgfh3vOCVpSIVgiZM5P0ZLQNEtIStJzdBHjJyGMUkYWaMFpwbjkS_S-DaCtGTwE3NhYj134wsZ6H_EudGOLuxZXzvc2xHu87tpyML37cf0BQ1tic-g7U4OH6Fpsq8qaPl6iswp8tFfzXaGPp8ft-iXZvD2_rh82Sc0y2Sep5obwLOcZEUymaUpLSKEUpOTSal2UFjg3uRCaZVRbmWkoBOMGNFhjTMFX6PaYuw_d92BjrxoX_z6H1nZDVDIXXJKCTuD1DA66saXaB9dAOKh5g8m_mX2IBnwVoDUu_mOSTCrEhN0dsdrt6tEFq2ID3k-hVI3jyApFmaJTH_4LBr10Og</recordid><startdate>19881201</startdate><enddate>19881201</enddate><creator>Perkins, TW</creator><creator>Alvarado, JA</creator><creator>Polansky, JR</creator><creator>Stilwell, L</creator><creator>Maglio, M</creator><creator>Juster, R</creator><general>ARVO</general><general>Association for Research in Vision and Ophtalmology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19881201</creationdate><title>Trabecular meshwork cells grown on filters. Conductivity and cytochalasin effects</title><author>Perkins, TW ; Alvarado, JA ; Polansky, JR ; Stilwell, L ; Maglio, M ; Juster, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h267t-4b3c03683605274441da4ad50d37ebb9dea33c855b261be76ba9523cabaeccc93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Biological Transport - drug effects</topic><topic>Body Fluids - drug effects</topic><topic>Body Fluids - metabolism</topic><topic>Cell Division</topic><topic>Cells, Cultured</topic><topic>Cytochalasin B - pharmacology</topic><topic>Eye and associated structures. Visual pathways and centers. Vision</topic><topic>Filtration - instrumentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Methods</topic><topic>Perfusion</topic><topic>Permeability</topic><topic>Trabecular Meshwork - cytology</topic><topic>Trabecular Meshwork - drug effects</topic><topic>Trabecular Meshwork - physiology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Perkins, TW</creatorcontrib><creatorcontrib>Alvarado, JA</creatorcontrib><creatorcontrib>Polansky, JR</creatorcontrib><creatorcontrib>Stilwell, L</creatorcontrib><creatorcontrib>Maglio, M</creatorcontrib><creatorcontrib>Juster, R</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Perkins, TW</au><au>Alvarado, JA</au><au>Polansky, JR</au><au>Stilwell, L</au><au>Maglio, M</au><au>Juster, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Trabecular meshwork cells grown on filters. Conductivity and cytochalasin effects</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>1988-12-01</date><risdate>1988</risdate><volume>29</volume><issue>12</issue><spage>1836</spage><epage>1846</epage><pages>1836-1846</pages><issn>0146-0404</issn><eissn>1552-5783</eissn><coden>IOVSDA</coden><abstract>A system was developed to measure the hydraulic conductivity of cultured monolayers of human trabecular meshwork (HTM) cells. By optimizing the cell growth conditions and evaluating a number of filter supports, confluent HTM cells in single layers were obtained for measurement of hydraulic conductivity. The HTM monolayers had hydraulic conductivities of 0.3-2.0 microliters/min/mm Hg/cm2 measured at near-physiological flow rates. Evaluations of cytochalasin B (CB) effects on the hydraulic conductivity of our HTM monolayers revealed that CB (10(-6) to 10(-5) M) caused a dramatic dose-related increase in conductivity within 10 to 30 min, which parallels CB effects on outflow facility in vivo. Morphologic observations show that the increase in hydraulic conductivity was accompanied by a retraction of the trabecular cells and widening of the intercellular spaces. Our findings suggest that growth of HTM cells on filter supports can provide a useful in vitro system to study the regulation of aqueous outflow.</abstract><cop>Rockville, MD</cop><pub>ARVO</pub><pmid>3192373</pmid><tpages>11</tpages></addata></record> |
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| identifier | ISSN: 0146-0404 |
| ispartof | Investigative ophthalmology & visual science, 1988-12, Vol.29 (12), p.1836-1846 |
| issn | 0146-0404 1552-5783 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adult Biological and medical sciences Biological Transport - drug effects Body Fluids - drug effects Body Fluids - metabolism Cell Division Cells, Cultured Cytochalasin B - pharmacology Eye and associated structures. Visual pathways and centers. Vision Filtration - instrumentation Fundamental and applied biological sciences. Psychology Humans Methods Perfusion Permeability Trabecular Meshwork - cytology Trabecular Meshwork - drug effects Trabecular Meshwork - physiology Vertebrates: nervous system and sense organs |
| title | Trabecular meshwork cells grown on filters. Conductivity and cytochalasin effects |
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