Comparison of gene probe and conventional methods for the differentiation of ovine footrot isolates of Dichelobacter nodosus
In a collaborative study that involved four Australian veterinary diagnostic laboratories a gene probe test based on the recombinant plasmids pJIR318, pJIR314B, and pJIR313, which contain genomic vap or vrl regions, was compared with conventional tests used for the differential diagnosis of ovine fo...
Gespeichert in:
| Veröffentlicht in: | Veterinary microbiology 1996-09, Vol.52 (1), p.127-141 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 141 |
|---|---|
| container_issue | 1 |
| container_start_page | 127 |
| container_title | Veterinary microbiology |
| container_volume | 52 |
| creator | Rood, Julian I. Howarth, Pauline A. Haring, Volker Billington, Stephen J. Yong, Weng K. Liu, Don Palmer, Michael A. Pitman, David R. Links, Ian Stewart, David J. Vaughan, Jill A. |
| description | In a collaborative study that involved four Australian veterinary diagnostic laboratories a gene probe test based on the recombinant plasmids pJIR318, pJIR314B, and pJIR313, which contain genomic
vap or
vrl regions, was compared with conventional tests used for the differential diagnosis of ovine footrot. A total of 771 clinical
Dichelobacter nodosus isolates were tested and designated as belonging to one of several gene probe categories. The results showed that 87% of the virulent isolates belonged to gene probe category 1, compared to only 6% of the benign isolates. It was concluded that there was good correlation between the gene probe test and the virulence designation of these isolates as well as the results of elastase, gelatin-gel and protease isoenzyme tests. Furthermore, the gene probe test was converted to a polymerase chain reaction (PCR)-based test. It is suggested that diagnostic laboratories consider carrying out both this PCR test and tests based on the extracellular proteases of
D. nodosus. |
| doi_str_mv | 10.1016/0378-1135(96)00054-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78530977</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0378113596000545</els_id><sourcerecordid>78530977</sourcerecordid><originalsourceid>FETCH-LOGICAL-c436t-1caa68fc5975e2202921717a316e0e80ac9e2dec788fee5ecf7a9670a4e373aa3</originalsourceid><addsrcrecordid>eNqFkU2LFDEYhBtR1tnVPyAKOYjooTUfnU7nsiCzfsGgFz2Hd9JvdiLdyZhkBgR_vGlmmKOeQqiniqSqaZ4z-pZR1r-jQg0tY0K-1v0bSqnsWvmgWbFBiZbLjj9sVhfkcXOd888KdbqnV83VoFnHpVo1f9Zx3kPyOQYSHbnHgGSf4hYJhJHYGI4Yio8BJjJj2cUxExcTKTsko3cO0yLDQiz2ePTV72IsKRZSQycomBflztsdTnELtmAiIY4xH_KT5pGDKePT83nT_Pj44fv6c7v59unL-v2mtZ3oS8ssQD84K7WSyDnlmjPFFAjWI8WBgtXIR7RqGByiROsU6F5R6FAoASBumlen3PqzXwfMxcw-W5wmCBgP2ahBCqqV-i_I5ND1SooKdifQpphzQmf2yc-QfhtGzbKOWao3S_VG18uyjpHV9uKcf9jOOF5M5zmq_vKsQ7YwuQTB-nzBBO-07nXFnp0wB9HAfV3PfN1oRTmjXRVvTyLWRo8ek8nWY7A4-oS2mDH6fz_yL5_9tZY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15846753</pqid></control><display><type>article</type><title>Comparison of gene probe and conventional methods for the differentiation of ovine footrot isolates of Dichelobacter nodosus</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Rood, Julian I. ; Howarth, Pauline A. ; Haring, Volker ; Billington, Stephen J. ; Yong, Weng K. ; Liu, Don ; Palmer, Michael A. ; Pitman, David R. ; Links, Ian ; Stewart, David J. ; Vaughan, Jill A.</creator><creatorcontrib>Rood, Julian I. ; Howarth, Pauline A. ; Haring, Volker ; Billington, Stephen J. ; Yong, Weng K. ; Liu, Don ; Palmer, Michael A. ; Pitman, David R. ; Links, Ian ; Stewart, David J. ; Vaughan, Jill A.</creatorcontrib><description>In a collaborative study that involved four Australian veterinary diagnostic laboratories a gene probe test based on the recombinant plasmids pJIR318, pJIR314B, and pJIR313, which contain genomic
vap or
vrl regions, was compared with conventional tests used for the differential diagnosis of ovine footrot. A total of 771 clinical
Dichelobacter nodosus isolates were tested and designated as belonging to one of several gene probe categories. The results showed that 87% of the virulent isolates belonged to gene probe category 1, compared to only 6% of the benign isolates. It was concluded that there was good correlation between the gene probe test and the virulence designation of these isolates as well as the results of elastase, gelatin-gel and protease isoenzyme tests. Furthermore, the gene probe test was converted to a polymerase chain reaction (PCR)-based test. It is suggested that diagnostic laboratories consider carrying out both this PCR test and tests based on the extracellular proteases of
D. nodosus.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/0378-1135(96)00054-5</identifier><identifier>PMID: 8914257</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>ADN RECOMBINADO ; ADN RECOMBINE ; Animal bacterial diseases ; Animals ; Bacterial diseases ; Base Sequence ; Biological and medical sciences ; DIAGNOSIS ; DIAGNOSTIC ; DIAGNOSTICO ; Dichelobacter nodosus ; DNA Primers ; DNA Probes ; Elastase ; FOOT ROT ; Foot Rot - microbiology ; Genes, Bacterial ; Gram-Negative Anaerobic Bacteria - genetics ; Gram-Negative Anaerobic Bacteria - isolation & purification ; Gram-Negative Anaerobic Bacteria - pathogenicity ; Gram-Negative Bacterial Infections - microbiology ; Gram-Negative Bacterial Infections - veterinary ; Hybridization ; Infectious diseases ; Medical sciences ; NUCLEIC PROBES ; OVIN ; OVINOS ; PCR ; PEDERO ; PLASMIDE ; PLASMIDIOS ; PLASMIDS ; PODODERMATITE INTERDIGITEE ; Polymerase Chain Reaction - methods ; RECOMBINANT DNA ; SHEEP ; Sheep Diseases ; SONDAS NUCLEICAS ; SONDE NUCLEIQUE ; vap ; Virulence ; vrl</subject><ispartof>Veterinary microbiology, 1996-09, Vol.52 (1), p.127-141</ispartof><rights>1996</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c436t-1caa68fc5975e2202921717a316e0e80ac9e2dec788fee5ecf7a9670a4e373aa3</citedby><cites>FETCH-LOGICAL-c436t-1caa68fc5975e2202921717a316e0e80ac9e2dec788fee5ecf7a9670a4e373aa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0378113596000545$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3249969$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8914257$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rood, Julian I.</creatorcontrib><creatorcontrib>Howarth, Pauline A.</creatorcontrib><creatorcontrib>Haring, Volker</creatorcontrib><creatorcontrib>Billington, Stephen J.</creatorcontrib><creatorcontrib>Yong, Weng K.</creatorcontrib><creatorcontrib>Liu, Don</creatorcontrib><creatorcontrib>Palmer, Michael A.</creatorcontrib><creatorcontrib>Pitman, David R.</creatorcontrib><creatorcontrib>Links, Ian</creatorcontrib><creatorcontrib>Stewart, David J.</creatorcontrib><creatorcontrib>Vaughan, Jill A.</creatorcontrib><title>Comparison of gene probe and conventional methods for the differentiation of ovine footrot isolates of Dichelobacter nodosus</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>In a collaborative study that involved four Australian veterinary diagnostic laboratories a gene probe test based on the recombinant plasmids pJIR318, pJIR314B, and pJIR313, which contain genomic
vap or
vrl regions, was compared with conventional tests used for the differential diagnosis of ovine footrot. A total of 771 clinical
Dichelobacter nodosus isolates were tested and designated as belonging to one of several gene probe categories. The results showed that 87% of the virulent isolates belonged to gene probe category 1, compared to only 6% of the benign isolates. It was concluded that there was good correlation between the gene probe test and the virulence designation of these isolates as well as the results of elastase, gelatin-gel and protease isoenzyme tests. Furthermore, the gene probe test was converted to a polymerase chain reaction (PCR)-based test. It is suggested that diagnostic laboratories consider carrying out both this PCR test and tests based on the extracellular proteases of
D. nodosus.</description><subject>ADN RECOMBINADO</subject><subject>ADN RECOMBINE</subject><subject>Animal bacterial diseases</subject><subject>Animals</subject><subject>Bacterial diseases</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>DIAGNOSIS</subject><subject>DIAGNOSTIC</subject><subject>DIAGNOSTICO</subject><subject>Dichelobacter nodosus</subject><subject>DNA Primers</subject><subject>DNA Probes</subject><subject>Elastase</subject><subject>FOOT ROT</subject><subject>Foot Rot - microbiology</subject><subject>Genes, Bacterial</subject><subject>Gram-Negative Anaerobic Bacteria - genetics</subject><subject>Gram-Negative Anaerobic Bacteria - isolation & purification</subject><subject>Gram-Negative Anaerobic Bacteria - pathogenicity</subject><subject>Gram-Negative Bacterial Infections - microbiology</subject><subject>Gram-Negative Bacterial Infections - veterinary</subject><subject>Hybridization</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>NUCLEIC PROBES</subject><subject>OVIN</subject><subject>OVINOS</subject><subject>PCR</subject><subject>PEDERO</subject><subject>PLASMIDE</subject><subject>PLASMIDIOS</subject><subject>PLASMIDS</subject><subject>PODODERMATITE INTERDIGITEE</subject><subject>Polymerase Chain Reaction - methods</subject><subject>RECOMBINANT DNA</subject><subject>SHEEP</subject><subject>Sheep Diseases</subject><subject>SONDAS NUCLEICAS</subject><subject>SONDE NUCLEIQUE</subject><subject>vap</subject><subject>Virulence</subject><subject>vrl</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2LFDEYhBtR1tnVPyAKOYjooTUfnU7nsiCzfsGgFz2Hd9JvdiLdyZhkBgR_vGlmmKOeQqiniqSqaZ4z-pZR1r-jQg0tY0K-1v0bSqnsWvmgWbFBiZbLjj9sVhfkcXOd888KdbqnV83VoFnHpVo1f9Zx3kPyOQYSHbnHgGSf4hYJhJHYGI4Yio8BJjJj2cUxExcTKTsko3cO0yLDQiz2ePTV72IsKRZSQycomBflztsdTnELtmAiIY4xH_KT5pGDKePT83nT_Pj44fv6c7v59unL-v2mtZ3oS8ssQD84K7WSyDnlmjPFFAjWI8WBgtXIR7RqGByiROsU6F5R6FAoASBumlen3PqzXwfMxcw-W5wmCBgP2ahBCqqV-i_I5ND1SooKdifQpphzQmf2yc-QfhtGzbKOWao3S_VG18uyjpHV9uKcf9jOOF5M5zmq_vKsQ7YwuQTB-nzBBO-07nXFnp0wB9HAfV3PfN1oRTmjXRVvTyLWRo8ek8nWY7A4-oS2mDH6fz_yL5_9tZY</recordid><startdate>19960901</startdate><enddate>19960901</enddate><creator>Rood, Julian I.</creator><creator>Howarth, Pauline A.</creator><creator>Haring, Volker</creator><creator>Billington, Stephen J.</creator><creator>Yong, Weng K.</creator><creator>Liu, Don</creator><creator>Palmer, Michael A.</creator><creator>Pitman, David R.</creator><creator>Links, Ian</creator><creator>Stewart, David J.</creator><creator>Vaughan, Jill A.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19960901</creationdate><title>Comparison of gene probe and conventional methods for the differentiation of ovine footrot isolates of Dichelobacter nodosus</title><author>Rood, Julian I. ; Howarth, Pauline A. ; Haring, Volker ; Billington, Stephen J. ; Yong, Weng K. ; Liu, Don ; Palmer, Michael A. ; Pitman, David R. ; Links, Ian ; Stewart, David J. ; Vaughan, Jill A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-1caa68fc5975e2202921717a316e0e80ac9e2dec788fee5ecf7a9670a4e373aa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>ADN RECOMBINADO</topic><topic>ADN RECOMBINE</topic><topic>Animal bacterial diseases</topic><topic>Animals</topic><topic>Bacterial diseases</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>DIAGNOSIS</topic><topic>DIAGNOSTIC</topic><topic>DIAGNOSTICO</topic><topic>Dichelobacter nodosus</topic><topic>DNA Primers</topic><topic>DNA Probes</topic><topic>Elastase</topic><topic>FOOT ROT</topic><topic>Foot Rot - microbiology</topic><topic>Genes, Bacterial</topic><topic>Gram-Negative Anaerobic Bacteria - genetics</topic><topic>Gram-Negative Anaerobic Bacteria - isolation & purification</topic><topic>Gram-Negative Anaerobic Bacteria - pathogenicity</topic><topic>Gram-Negative Bacterial Infections - microbiology</topic><topic>Gram-Negative Bacterial Infections - veterinary</topic><topic>Hybridization</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>NUCLEIC PROBES</topic><topic>OVIN</topic><topic>OVINOS</topic><topic>PCR</topic><topic>PEDERO</topic><topic>PLASMIDE</topic><topic>PLASMIDIOS</topic><topic>PLASMIDS</topic><topic>PODODERMATITE INTERDIGITEE</topic><topic>Polymerase Chain Reaction - methods</topic><topic>RECOMBINANT DNA</topic><topic>SHEEP</topic><topic>Sheep Diseases</topic><topic>SONDAS NUCLEICAS</topic><topic>SONDE NUCLEIQUE</topic><topic>vap</topic><topic>Virulence</topic><topic>vrl</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rood, Julian I.</creatorcontrib><creatorcontrib>Howarth, Pauline A.</creatorcontrib><creatorcontrib>Haring, Volker</creatorcontrib><creatorcontrib>Billington, Stephen J.</creatorcontrib><creatorcontrib>Yong, Weng K.</creatorcontrib><creatorcontrib>Liu, Don</creatorcontrib><creatorcontrib>Palmer, Michael A.</creatorcontrib><creatorcontrib>Pitman, David R.</creatorcontrib><creatorcontrib>Links, Ian</creatorcontrib><creatorcontrib>Stewart, David J.</creatorcontrib><creatorcontrib>Vaughan, Jill A.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rood, Julian I.</au><au>Howarth, Pauline A.</au><au>Haring, Volker</au><au>Billington, Stephen J.</au><au>Yong, Weng K.</au><au>Liu, Don</au><au>Palmer, Michael A.</au><au>Pitman, David R.</au><au>Links, Ian</au><au>Stewart, David J.</au><au>Vaughan, Jill A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of gene probe and conventional methods for the differentiation of ovine footrot isolates of Dichelobacter nodosus</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>1996-09-01</date><risdate>1996</risdate><volume>52</volume><issue>1</issue><spage>127</spage><epage>141</epage><pages>127-141</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>In a collaborative study that involved four Australian veterinary diagnostic laboratories a gene probe test based on the recombinant plasmids pJIR318, pJIR314B, and pJIR313, which contain genomic
vap or
vrl regions, was compared with conventional tests used for the differential diagnosis of ovine footrot. A total of 771 clinical
Dichelobacter nodosus isolates were tested and designated as belonging to one of several gene probe categories. The results showed that 87% of the virulent isolates belonged to gene probe category 1, compared to only 6% of the benign isolates. It was concluded that there was good correlation between the gene probe test and the virulence designation of these isolates as well as the results of elastase, gelatin-gel and protease isoenzyme tests. Furthermore, the gene probe test was converted to a polymerase chain reaction (PCR)-based test. It is suggested that diagnostic laboratories consider carrying out both this PCR test and tests based on the extracellular proteases of
D. nodosus.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>8914257</pmid><doi>10.1016/0378-1135(96)00054-5</doi><tpages>15</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1135 |
| ispartof | Veterinary microbiology, 1996-09, Vol.52 (1), p.127-141 |
| issn | 0378-1135 1873-2542 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78530977 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | ADN RECOMBINADO ADN RECOMBINE Animal bacterial diseases Animals Bacterial diseases Base Sequence Biological and medical sciences DIAGNOSIS DIAGNOSTIC DIAGNOSTICO Dichelobacter nodosus DNA Primers DNA Probes Elastase FOOT ROT Foot Rot - microbiology Genes, Bacterial Gram-Negative Anaerobic Bacteria - genetics Gram-Negative Anaerobic Bacteria - isolation & purification Gram-Negative Anaerobic Bacteria - pathogenicity Gram-Negative Bacterial Infections - microbiology Gram-Negative Bacterial Infections - veterinary Hybridization Infectious diseases Medical sciences NUCLEIC PROBES OVIN OVINOS PCR PEDERO PLASMIDE PLASMIDIOS PLASMIDS PODODERMATITE INTERDIGITEE Polymerase Chain Reaction - methods RECOMBINANT DNA SHEEP Sheep Diseases SONDAS NUCLEICAS SONDE NUCLEIQUE vap Virulence vrl |
| title | Comparison of gene probe and conventional methods for the differentiation of ovine footrot isolates of Dichelobacter nodosus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T09%3A45%3A37IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20gene%20probe%20and%20conventional%20methods%20for%20the%20differentiation%20of%20ovine%20footrot%20isolates%20of%20Dichelobacter%20nodosus&rft.jtitle=Veterinary%20microbiology&rft.au=Rood,%20Julian%20I.&rft.date=1996-09-01&rft.volume=52&rft.issue=1&rft.spage=127&rft.epage=141&rft.pages=127-141&rft.issn=0378-1135&rft.eissn=1873-2542&rft.coden=VMICDQ&rft_id=info:doi/10.1016/0378-1135(96)00054-5&rft_dat=%3Cproquest_cross%3E78530977%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15846753&rft_id=info:pmid/8914257&rft_els_id=0378113596000545&rfr_iscdi=true |