Detection and sequence analysis of the major immediate early and PP150 gene of latent human cytomegalovirus in spleen, liver, and kidney tissues of trauma victims
The presence of human cytomegalovirus (HCMV) DNA in liver, spleen, and kidney samples of HCMV‐seropositive trauma victims during latency was demonstrated by polymerase chain reaction (PCR), using primers reactive with the major immediate early gene exon 4 and the structural gene pp150. Sequence anal...
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| Veröffentlicht in: | Journal of medical virology 1996-10, Vol.50 (2), p.193-197 |
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| creator | Hendrix, M. G. Ron Beuken, Eric Slobbe, Rob L. Bruggeman, Cathrien A. |
| description | The presence of human cytomegalovirus (HCMV) DNA in liver, spleen, and kidney samples of HCMV‐seropositive trauma victims during latency was demonstrated by polymerase chain reaction (PCR), using primers reactive with the major immediate early gene exon 4 and the structural gene pp150. Sequence analysis of the PCR amplificates showed more than 95% homology with the reference HCMV strain AD169. The few mutations observed were mostly distributed randomly. In one subject two types of the MIE‐4 gene were detected, and in another subject two types of the pp150 gene were found, suggesting that different strains of HCMV can be found in organs of the same patient during latency. © 1996 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/(SICI)1096-9071(199610)50:2<193::AID-JMV14>3.0.CO;2-N |
| format | Article |
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G. Ron ; Beuken, Eric ; Slobbe, Rob L. ; Bruggeman, Cathrien A.</creator><creatorcontrib>Hendrix, M. G. Ron ; Beuken, Eric ; Slobbe, Rob L. ; Bruggeman, Cathrien A.</creatorcontrib><description>The presence of human cytomegalovirus (HCMV) DNA in liver, spleen, and kidney samples of HCMV‐seropositive trauma victims during latency was demonstrated by polymerase chain reaction (PCR), using primers reactive with the major immediate early gene exon 4 and the structural gene pp150. Sequence analysis of the PCR amplificates showed more than 95% homology with the reference HCMV strain AD169. The few mutations observed were mostly distributed randomly. 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G. Ron</creatorcontrib><creatorcontrib>Beuken, Eric</creatorcontrib><creatorcontrib>Slobbe, Rob L.</creatorcontrib><creatorcontrib>Bruggeman, Cathrien A.</creatorcontrib><title>Detection and sequence analysis of the major immediate early and PP150 gene of latent human cytomegalovirus in spleen, liver, and kidney tissues of trauma victims</title><title>Journal of medical virology</title><addtitle>J. Med. Virol</addtitle><description>The presence of human cytomegalovirus (HCMV) DNA in liver, spleen, and kidney samples of HCMV‐seropositive trauma victims during latency was demonstrated by polymerase chain reaction (PCR), using primers reactive with the major immediate early gene exon 4 and the structural gene pp150. Sequence analysis of the PCR amplificates showed more than 95% homology with the reference HCMV strain AD169. The few mutations observed were mostly distributed randomly. In one subject two types of the MIE‐4 gene were detected, and in another subject two types of the pp150 gene were found, suggesting that different strains of HCMV can be found in organs of the same patient during latency. © 1996 Wiley‐Liss, Inc.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Cytomegalovirus - isolation & purification</subject><subject>Cytomegalovirus - physiology</subject><subject>Cytomegalovirus Infections - blood</subject><subject>Cytomegalovirus Infections - virology</subject><subject>DNA, Viral - analysis</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HCMV</subject><subject>Humans</subject><subject>Kidney - virology</subject><subject>latency</subject><subject>Liver - virology</subject><subject>Male</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>PCR</subject><subject>Phosphoproteins</subject><subject>Polymerase Chain Reaction</subject><subject>Sequence Analysis, DNA</subject><subject>Spleen - virology</subject><subject>Techniques used in virology</subject><subject>Viral Matrix Proteins - genetics</subject><subject>Virology</subject><subject>Virus Latency</subject><subject>Wounds and Injuries - virology</subject><issn>0146-6615</issn><issn>1096-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1v0zAUhiMEGmXwE5B8gdAmLcUfdRJ3gLR1MDqVdtJgk7ix3OR08-Y4xU4K-Tv8Utym6g1Iu_HHOe95_cpPFH0guE8wpu8Orsaj8SHBIokFTskBESIh-JDjIX1PBBsOT8Zn8cXXazL4yPq4P5od03j6JOrtJp5GPUwGSZwkhD-PXnh_jzHOBKV70V4mCM-ytBf9OYMa8lpXFilbIA8_G7A5hIsyrdceVQtU3wEq1X3lkC5LKLSqAYFypt2MXF4SjtEtWFhrTWjaGt01pbIob-uqhFtlqpV2jUfaIr80APYIGb0Cd7QxeNCFhRbV2vsGugedCvNopUOw0r-Mni2U8fBqu-9H3z9_-jb6Ek9m5-PRySTOBwwP4izhoKhiKckhZbRI53ies4ECAMwpF_OCCq4KNucFDwtf0EwJlrEMlKBJSth-9LbzXboq_IKvZal9DsYoC1XjZZpxhjnGbBcgd5X3DhZy6XSpXCsJlmt2Uq7ZyTUJuSYhO3aSY0nDmUkZ2MkNO8kklqNZqE-D7-ttgGYe_nnnuoUV-m-2feVzZRZO2Vz7nYzRhGY0CbLrTvZLG2j_yfZItP8l6wrBOO6Mta_h985YuQeZpCzl8mZ6Lm_Y6dUpYz_khP0FkXDSZg</recordid><startdate>199610</startdate><enddate>199610</enddate><creator>Hendrix, M. G. Ron</creator><creator>Beuken, Eric</creator><creator>Slobbe, Rob L.</creator><creator>Bruggeman, Cathrien A.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199610</creationdate><title>Detection and sequence analysis of the major immediate early and PP150 gene of latent human cytomegalovirus in spleen, liver, and kidney tissues of trauma victims</title><author>Hendrix, M. G. Ron ; Beuken, Eric ; Slobbe, Rob L. ; Bruggeman, Cathrien A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4304-865ea2a371ce732d7b0bc34aeee05259bd295ad3b5d53b55f28a93838ea926713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Cytomegalovirus - isolation & purification</topic><topic>Cytomegalovirus - physiology</topic><topic>Cytomegalovirus Infections - blood</topic><topic>Cytomegalovirus Infections - virology</topic><topic>DNA, Viral - analysis</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. 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Ron</creatorcontrib><creatorcontrib>Beuken, Eric</creatorcontrib><creatorcontrib>Slobbe, Rob L.</creatorcontrib><creatorcontrib>Bruggeman, Cathrien A.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hendrix, M. G. Ron</au><au>Beuken, Eric</au><au>Slobbe, Rob L.</au><au>Bruggeman, Cathrien A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection and sequence analysis of the major immediate early and PP150 gene of latent human cytomegalovirus in spleen, liver, and kidney tissues of trauma victims</atitle><jtitle>Journal of medical virology</jtitle><addtitle>J. Med. Virol</addtitle><date>1996-10</date><risdate>1996</risdate><volume>50</volume><issue>2</issue><spage>193</spage><epage>197</epage><pages>193-197</pages><issn>0146-6615</issn><eissn>1096-9071</eissn><coden>JMVIDB</coden><abstract>The presence of human cytomegalovirus (HCMV) DNA in liver, spleen, and kidney samples of HCMV‐seropositive trauma victims during latency was demonstrated by polymerase chain reaction (PCR), using primers reactive with the major immediate early gene exon 4 and the structural gene pp150. Sequence analysis of the PCR amplificates showed more than 95% homology with the reference HCMV strain AD169. The few mutations observed were mostly distributed randomly. In one subject two types of the MIE‐4 gene were detected, and in another subject two types of the pp150 gene were found, suggesting that different strains of HCMV can be found in organs of the same patient during latency. © 1996 Wiley‐Liss, Inc.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>8915887</pmid><doi>10.1002/(SICI)1096-9071(199610)50:2<193::AID-JMV14>3.0.CO;2-N</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0146-6615 |
| ispartof | Journal of medical virology, 1996-10, Vol.50 (2), p.193-197 |
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| subjects | Adolescent Adult Biological and medical sciences Cytomegalovirus - isolation & purification Cytomegalovirus - physiology Cytomegalovirus Infections - blood Cytomegalovirus Infections - virology DNA, Viral - analysis Female Fundamental and applied biological sciences. Psychology HCMV Humans Kidney - virology latency Liver - virology Male Microbiology Middle Aged PCR Phosphoproteins Polymerase Chain Reaction Sequence Analysis, DNA Spleen - virology Techniques used in virology Viral Matrix Proteins - genetics Virology Virus Latency Wounds and Injuries - virology |
| title | Detection and sequence analysis of the major immediate early and PP150 gene of latent human cytomegalovirus in spleen, liver, and kidney tissues of trauma victims |
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