Direct evidence for the presence of GABAA receptors on the cytoplasmic side of the Deiters' neurone membrane
A newly developed micromethod has been used for studying the rate of passage of 36Cl- ions across single nerve membranes from rabbit Deiters' neurones. The application of gamma-aminobutyric acid (GABA) on the cytoplasmic side of those membranes increases the rate of passage of 36Cl- ions from t...
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| Veröffentlicht in: | Brain research 1988-10, Vol.462 (2), p.350-353 |
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| creator | RAPALLINO, M. V CUPELLO, A HYDEN, H |
| description | A newly developed micromethod has been used for studying the rate of passage of 36Cl- ions across single nerve membranes from rabbit Deiters' neurones. The application of gamma-aminobutyric acid (GABA) on the cytoplasmic side of those membranes increases the rate of passage of 36Cl- ions from that side to the other one across the membrane. The maximal effect is exerted by 10(-6) M GABA and it fades at higher neurotransmitter concentrations (10(-5) M to 3.3 x 10(-3) M). The cause of this fading of the effect appears to be a receptor desensitization phenomenon. The 10(-6) M GABA effect is reversed by both 10(-4) M picrotoxin and 10(-5) M bicuculline. The overall pattern of the data indicates the presence of GABAA receptors on the cytoplasmic side of these nerve membranes. |
| doi_str_mv | 10.1016/0006-8993(88)90563-X |
| format | Article |
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The overall pattern of the data indicates the presence of GABAA receptors on the cytoplasmic side of these nerve membranes.</description><subject>Animals</subject><subject>Bicuculline - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>Chlorides - pharmacokinetics</subject><subject>Cytoplasm - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gamma-Aminobutyric Acid - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Neurons - drug effects</subject><subject>Neurons - metabolism</subject><subject>Picrotoxin - pharmacology</subject><subject>Rabbits</subject><subject>Receptors, GABA-A - drug effects</subject><subject>Receptors, GABA-A - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LxDAQhoMouq7-A4UcxI9DNWnafBzXXV2FBS8evIU0nWClbWrSCv5723XZU8jM8w4zD0IXlNxTQvkDIYQnUil2K-WdIjlnyccBmlEp0oSnGTlEsz1ygk5j_Bq_jClyjI5TmQmZpzNUr6oAtsfwU5XQWsDOB9x_Au4CxG3BO7xePC4WeOSg632I2LdbxP72vqtNbCqL4xif0Km-gqqHEG9wC0PwLeAGmiKYFs7QkTN1hPPdO0fvz0_vy5dk87Z-XS42iU0z3ic8p0JSIYxwhStyZzIrVClVkTLGKJG8cBknjlBFeOZKIRzPnaWMGVrmqmBzdP0_tgv-e4DY66aKFup6XMEPUU-Xq1yKEcz-QRt8jAGc7kLVmPCrKdGTYz0J1JNALaXeOtYfY-xyN38oGij3oZ3UsX-165toTe3G020V95ggnCqRsT_BH4Pq</recordid><startdate>19881018</startdate><enddate>19881018</enddate><creator>RAPALLINO, M. 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Pathways and receptors</topic><topic>Chlorides - pharmacokinetics</topic><topic>Cytoplasm - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gamma-Aminobutyric Acid - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Neurons - drug effects</topic><topic>Neurons - metabolism</topic><topic>Picrotoxin - pharmacology</topic><topic>Rabbits</topic><topic>Receptors, GABA-A - drug effects</topic><topic>Receptors, GABA-A - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>RAPALLINO, M. V</creatorcontrib><creatorcontrib>CUPELLO, A</creatorcontrib><creatorcontrib>HYDEN, H</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>RAPALLINO, M. V</au><au>CUPELLO, A</au><au>HYDEN, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct evidence for the presence of GABAA receptors on the cytoplasmic side of the Deiters' neurone membrane</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>1988-10-18</date><risdate>1988</risdate><volume>462</volume><issue>2</issue><spage>350</spage><epage>353</epage><pages>350-353</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>A newly developed micromethod has been used for studying the rate of passage of 36Cl- ions across single nerve membranes from rabbit Deiters' neurones. The application of gamma-aminobutyric acid (GABA) on the cytoplasmic side of those membranes increases the rate of passage of 36Cl- ions from that side to the other one across the membrane. The maximal effect is exerted by 10(-6) M GABA and it fades at higher neurotransmitter concentrations (10(-5) M to 3.3 x 10(-3) M). The cause of this fading of the effect appears to be a receptor desensitization phenomenon. The 10(-6) M GABA effect is reversed by both 10(-4) M picrotoxin and 10(-5) M bicuculline. The overall pattern of the data indicates the presence of GABAA receptors on the cytoplasmic side of these nerve membranes.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier</pub><pmid>2847852</pmid><doi>10.1016/0006-8993(88)90563-X</doi><tpages>4</tpages></addata></record> |
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| ispartof | Brain research, 1988-10, Vol.462 (2), p.350-353 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Bicuculline - pharmacology Biological and medical sciences Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Chlorides - pharmacokinetics Cytoplasm - metabolism Fundamental and applied biological sciences. Psychology gamma-Aminobutyric Acid - pharmacology In Vitro Techniques Neurons - drug effects Neurons - metabolism Picrotoxin - pharmacology Rabbits Receptors, GABA-A - drug effects Receptors, GABA-A - metabolism Vertebrates: nervous system and sense organs |
| title | Direct evidence for the presence of GABAA receptors on the cytoplasmic side of the Deiters' neurone membrane |
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