The use of plasmid profile analysis and ribotyping for typing Acinetobacter baumannii isolates
Plasmid profiles were used to analyse 39 Acinetobacter baumannii isolates from 36 patients at three hospitals. The isolates were previously classified by biotyping and rDNA fingerprinting. Ribotyping was useful to establish the lineage of isolates and to confirm genospecies identification. Thirty-se...
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| Veröffentlicht in: | The Journal of hospital infection 1996-10, Vol.34 (2), p.139-144 |
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| container_title | The Journal of hospital infection |
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| creator | García, D.C. Nociari, M.M. Sordelli, D.O. Di Martino, A. Catalano, M. |
| description | Plasmid profiles were used to analyse 39
Acinetobacter baumannii isolates from 36 patients at three hospitals. The isolates were previously classified by biotyping and rDNA fingerprinting. Ribotyping was useful to establish the lineage of isolates and to confirm genospecies identification. Thirty-seven isolates (94·9%) contained plasmids. The variable number of plasmids with different molecular weights in each isolate enabled the identification of 13 profiles without the need for endonuclease digestion. Fifteen
A. baumannii biotype 2 isolates of similar ribotype and antibiotype contained identical plasmids over a two-month outbreak at one hospital. Plasmid typing discriminated these isolates from sporadic
A. baumannii isolates of close ribotype obtained from different hospitals. A few isolates of different lineage, however, showed similar plasmid profile. Our results suggest that plasmid typing is a practical method to assist infection control of nosocomial
A. baumannii. A combination of plasmid typing and ribotyping is suggested to confirm genospecies classification and to identify strains against reference band profiles. |
| doi_str_mv | 10.1016/S0195-6701(96)90139-5 |
| format | Article |
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Acinetobacter baumannii isolates from 36 patients at three hospitals. The isolates were previously classified by biotyping and rDNA fingerprinting. Ribotyping was useful to establish the lineage of isolates and to confirm genospecies identification. Thirty-seven isolates (94·9%) contained plasmids. The variable number of plasmids with different molecular weights in each isolate enabled the identification of 13 profiles without the need for endonuclease digestion. Fifteen
A. baumannii biotype 2 isolates of similar ribotype and antibiotype contained identical plasmids over a two-month outbreak at one hospital. Plasmid typing discriminated these isolates from sporadic
A. baumannii isolates of close ribotype obtained from different hospitals. A few isolates of different lineage, however, showed similar plasmid profile. Our results suggest that plasmid typing is a practical method to assist infection control of nosocomial
A. baumannii. A combination of plasmid typing and ribotyping is suggested to confirm genospecies classification and to identify strains against reference band profiles.</description><identifier>ISSN: 0195-6701</identifier><identifier>EISSN: 1532-2939</identifier><identifier>DOI: 10.1016/S0195-6701(96)90139-5</identifier><identifier>PMID: 8910756</identifier><language>eng</language><publisher>Kent: Elsevier Ltd</publisher><subject>Acinetobacter ; Acinetobacter - classification ; Acinetobacter - drug effects ; Acinetobacter - isolation & purification ; Acinetobacter baumannii ; Bacteriological methods and techniques used in bacteriology ; Bacteriological Techniques ; Bacteriology ; Biological and medical sciences ; Cross Infection - microbiology ; Fundamental and applied biological sciences. Psychology ; Humans ; Microbial Sensitivity Tests ; Microbiology ; Plasmid profile ; Plasmids - classification ; Plasmids - isolation & purification ; ribotyping ; strain identification ; Tropical medicine</subject><ispartof>The Journal of hospital infection, 1996-10, Vol.34 (2), p.139-144</ispartof><rights>1996</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-4ee91c80d2e275a6d31a3c1d1b5d9efd12b275f3ecfdce31c4ac2c953eb1ce5e3</citedby><cites>FETCH-LOGICAL-c420t-4ee91c80d2e275a6d31a3c1d1b5d9efd12b275f3ecfdce31c4ac2c953eb1ce5e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0195670196901395$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3242758$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8910756$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>García, D.C.</creatorcontrib><creatorcontrib>Nociari, M.M.</creatorcontrib><creatorcontrib>Sordelli, D.O.</creatorcontrib><creatorcontrib>Di Martino, A.</creatorcontrib><creatorcontrib>Catalano, M.</creatorcontrib><title>The use of plasmid profile analysis and ribotyping for typing Acinetobacter baumannii isolates</title><title>The Journal of hospital infection</title><addtitle>J Hosp Infect</addtitle><description>Plasmid profiles were used to analyse 39
Acinetobacter baumannii isolates from 36 patients at three hospitals. The isolates were previously classified by biotyping and rDNA fingerprinting. Ribotyping was useful to establish the lineage of isolates and to confirm genospecies identification. Thirty-seven isolates (94·9%) contained plasmids. The variable number of plasmids with different molecular weights in each isolate enabled the identification of 13 profiles without the need for endonuclease digestion. Fifteen
A. baumannii biotype 2 isolates of similar ribotype and antibiotype contained identical plasmids over a two-month outbreak at one hospital. Plasmid typing discriminated these isolates from sporadic
A. baumannii isolates of close ribotype obtained from different hospitals. A few isolates of different lineage, however, showed similar plasmid profile. Our results suggest that plasmid typing is a practical method to assist infection control of nosocomial
A. baumannii. A combination of plasmid typing and ribotyping is suggested to confirm genospecies classification and to identify strains against reference band profiles.</description><subject>Acinetobacter</subject><subject>Acinetobacter - classification</subject><subject>Acinetobacter - drug effects</subject><subject>Acinetobacter - isolation & purification</subject><subject>Acinetobacter baumannii</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriological Techniques</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cross Infection - microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbiology</subject><subject>Plasmid profile</subject><subject>Plasmids - classification</subject><subject>Plasmids - isolation & purification</subject><subject>ribotyping</subject><subject>strain identification</subject><subject>Tropical medicine</subject><issn>0195-6701</issn><issn>1532-2939</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EKtvCI1TyASE4BDx2nMQnVFVQkCpxoFyxnPEEjJJ4sZNK-_a43WivPc1o5pvx-P8ZuwTxAQQ0H38IMLpqWgHvTPPeCFCm0s_YDrSSlTTKPGe7E_KSnef8VwhR6vqMnXUGRKubHft194f4monHge9Hl6fg-T7FIYzE3ezGQw65JJ6n0MflsA_zbz7ExLf0CsNMS-wdLpR479bJzXMIPOQ4uoXyK_ZicGOm11u8YD-_fL67_lrdfr_5dn11W2EtxVLVRAawE16SbLVrvAKnEDz02hsaPMi-1AdFOHgkBVg7lGi0oh6QNKkL9va4t9z-b6W82ClkpHF0M8U127bTsm6FfBIEbVplOiigPoKYYs6JBrtPYXLpYEHYBwPsowH2QV1rGvtogNVl7nJ7YO0n8qepTfHSf7P1XUY3DsnNGPIJU7IuP-0K9umIUVHtPlCyGQPNSD4kwsX6GJ445D-HnaQz</recordid><startdate>19961001</startdate><enddate>19961001</enddate><creator>García, D.C.</creator><creator>Nociari, M.M.</creator><creator>Sordelli, D.O.</creator><creator>Di Martino, A.</creator><creator>Catalano, M.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19961001</creationdate><title>The use of plasmid profile analysis and ribotyping for typing Acinetobacter baumannii isolates</title><author>García, D.C. ; Nociari, M.M. ; Sordelli, D.O. ; Di Martino, A. ; Catalano, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-4ee91c80d2e275a6d31a3c1d1b5d9efd12b275f3ecfdce31c4ac2c953eb1ce5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Acinetobacter</topic><topic>Acinetobacter - classification</topic><topic>Acinetobacter - drug effects</topic><topic>Acinetobacter - isolation & purification</topic><topic>Acinetobacter baumannii</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriological Techniques</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cross Infection - microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbiology</topic><topic>Plasmid profile</topic><topic>Plasmids - classification</topic><topic>Plasmids - isolation & purification</topic><topic>ribotyping</topic><topic>strain identification</topic><topic>Tropical medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>García, D.C.</creatorcontrib><creatorcontrib>Nociari, M.M.</creatorcontrib><creatorcontrib>Sordelli, D.O.</creatorcontrib><creatorcontrib>Di Martino, A.</creatorcontrib><creatorcontrib>Catalano, M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of hospital infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>García, D.C.</au><au>Nociari, M.M.</au><au>Sordelli, D.O.</au><au>Di Martino, A.</au><au>Catalano, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The use of plasmid profile analysis and ribotyping for typing Acinetobacter baumannii isolates</atitle><jtitle>The Journal of hospital infection</jtitle><addtitle>J Hosp Infect</addtitle><date>1996-10-01</date><risdate>1996</risdate><volume>34</volume><issue>2</issue><spage>139</spage><epage>144</epage><pages>139-144</pages><issn>0195-6701</issn><eissn>1532-2939</eissn><abstract>Plasmid profiles were used to analyse 39
Acinetobacter baumannii isolates from 36 patients at three hospitals. The isolates were previously classified by biotyping and rDNA fingerprinting. Ribotyping was useful to establish the lineage of isolates and to confirm genospecies identification. Thirty-seven isolates (94·9%) contained plasmids. The variable number of plasmids with different molecular weights in each isolate enabled the identification of 13 profiles without the need for endonuclease digestion. Fifteen
A. baumannii biotype 2 isolates of similar ribotype and antibiotype contained identical plasmids over a two-month outbreak at one hospital. Plasmid typing discriminated these isolates from sporadic
A. baumannii isolates of close ribotype obtained from different hospitals. A few isolates of different lineage, however, showed similar plasmid profile. Our results suggest that plasmid typing is a practical method to assist infection control of nosocomial
A. baumannii. A combination of plasmid typing and ribotyping is suggested to confirm genospecies classification and to identify strains against reference band profiles.</abstract><cop>Kent</cop><pub>Elsevier Ltd</pub><pmid>8910756</pmid><doi>10.1016/S0195-6701(96)90139-5</doi><tpages>6</tpages></addata></record> |
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| ispartof | The Journal of hospital infection, 1996-10, Vol.34 (2), p.139-144 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Acinetobacter Acinetobacter - classification Acinetobacter - drug effects Acinetobacter - isolation & purification Acinetobacter baumannii Bacteriological methods and techniques used in bacteriology Bacteriological Techniques Bacteriology Biological and medical sciences Cross Infection - microbiology Fundamental and applied biological sciences. Psychology Humans Microbial Sensitivity Tests Microbiology Plasmid profile Plasmids - classification Plasmids - isolation & purification ribotyping strain identification Tropical medicine |
| title | The use of plasmid profile analysis and ribotyping for typing Acinetobacter baumannii isolates |
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