Effect of cholesterol and other sterols on human sperm acrosomal responsiveness
Human sperm become responsive to inducers of the acrosome reaction when they are washed free of seminal plasma and incubated in an appropriate medium. Previous work has shown that cholesterol‐enriched medium prevents sperm from becoming responsive to the inducer, progesterone. Sperm that were incuba...
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| Veröffentlicht in: | Molecular reproduction and development 1996-10, Vol.45 (2), p.212-217 |
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| container_title | Molecular reproduction and development |
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| creator | Cross, Nicholas L. |
| description | Human sperm become responsive to inducers of the acrosome reaction when they are washed free of seminal plasma and incubated in an appropriate medium. Previous work has shown that cholesterol‐enriched medium prevents sperm from becoming responsive to the inducer, progesterone. Sperm that were incubated 24 hr in cholesterol‐enriched medium and then treated with progesterone showed no evidence of membrane fusion, indicating that cholesterol acts at a stage before the earliest morphological change. Inhibition of acrosomal responsiveness by cholesterol was reversible. Among other sterols reported in mammalian sperm, desmosterol and cholesterol sulfate also inhibited sperm from becoming responsive, but cholesterol palmitate did not. Our results support a model in which sperm unesterified cholesterol, or a molecule in equilibrium with it, suppresses acrosomal responsiveness. Cholesterol‐enriched medium also prevented sperm from becoming responsive to the calcium/proton exchanging ionophore, ionomycin, suggesting that cholesterol's effect may be, at least in part, at a point in the signal transduction pathway subsequent to the rise in intracellular‐free calcium. © 1996 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/(SICI)1098-2795(199610)45:2<212::AID-MRD14>3.0.CO;2-2 |
| format | Article |
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Previous work has shown that cholesterol‐enriched medium prevents sperm from becoming responsive to the inducer, progesterone. Sperm that were incubated 24 hr in cholesterol‐enriched medium and then treated with progesterone showed no evidence of membrane fusion, indicating that cholesterol acts at a stage before the earliest morphological change. Inhibition of acrosomal responsiveness by cholesterol was reversible. Among other sterols reported in mammalian sperm, desmosterol and cholesterol sulfate also inhibited sperm from becoming responsive, but cholesterol palmitate did not. Our results support a model in which sperm unesterified cholesterol, or a molecule in equilibrium with it, suppresses acrosomal responsiveness. Cholesterol‐enriched medium also prevented sperm from becoming responsive to the calcium/proton exchanging ionophore, ionomycin, suggesting that cholesterol's effect may be, at least in part, at a point in the signal transduction pathway subsequent to the rise in intracellular‐free calcium. © 1996 Wiley‐Liss, Inc.</description><identifier>ISSN: 1040-452X</identifier><identifier>EISSN: 1098-2795</identifier><identifier>DOI: 10.1002/(SICI)1098-2795(199610)45:2<212::AID-MRD14>3.0.CO;2-2</identifier><identifier>PMID: 8914079</identifier><identifier>CODEN: MREDEE</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>acrosome ; Acrosome - drug effects ; acrosome reaction ; Biological and medical sciences ; capacitation ; Cholesterol - pharmacology ; Fundamental and applied biological sciences. Psychology ; Humans ; Male ; Mammalian male genital system ; Morphology. Physiology ; Sperm Capacitation - drug effects ; Spermatozoa - drug effects ; Spermatozoa - physiology ; Sterols - pharmacology ; Vertebrates: reproduction</subject><ispartof>Molecular reproduction and development, 1996-10, Vol.45 (2), p.212-217</ispartof><rights>Copyright © 1996 Wiley‐Liss, Inc.</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c4964-3efd1afb58e0544ffb5444df5f7a47bf5bb712a0d99ac69129f6d90bd0e23ad93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291098-2795%28199610%2945%3A2%3C212%3A%3AAID-MRD14%3E3.0.CO%3B2-2$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291098-2795%28199610%2945%3A2%3C212%3A%3AAID-MRD14%3E3.0.CO%3B2-2$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3260724$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8914079$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cross, Nicholas L.</creatorcontrib><title>Effect of cholesterol and other sterols on human sperm acrosomal responsiveness</title><title>Molecular reproduction and development</title><addtitle>Mol. Reprod. Dev</addtitle><description>Human sperm become responsive to inducers of the acrosome reaction when they are washed free of seminal plasma and incubated in an appropriate medium. Previous work has shown that cholesterol‐enriched medium prevents sperm from becoming responsive to the inducer, progesterone. Sperm that were incubated 24 hr in cholesterol‐enriched medium and then treated with progesterone showed no evidence of membrane fusion, indicating that cholesterol acts at a stage before the earliest morphological change. Inhibition of acrosomal responsiveness by cholesterol was reversible. Among other sterols reported in mammalian sperm, desmosterol and cholesterol sulfate also inhibited sperm from becoming responsive, but cholesterol palmitate did not. Our results support a model in which sperm unesterified cholesterol, or a molecule in equilibrium with it, suppresses acrosomal responsiveness. Cholesterol‐enriched medium also prevented sperm from becoming responsive to the calcium/proton exchanging ionophore, ionomycin, suggesting that cholesterol's effect may be, at least in part, at a point in the signal transduction pathway subsequent to the rise in intracellular‐free calcium. © 1996 Wiley‐Liss, Inc.</description><subject>acrosome</subject><subject>Acrosome - drug effects</subject><subject>acrosome reaction</subject><subject>Biological and medical sciences</subject><subject>capacitation</subject><subject>Cholesterol - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Morphology. Physiology</subject><subject>Sperm Capacitation - drug effects</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - physiology</subject><subject>Sterols - pharmacology</subject><subject>Vertebrates: reproduction</subject><issn>1040-452X</issn><issn>1098-2795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVtv1DAQRiMEKm3hJyD5AaH2Icv4lsTLRSrphZUWVqJcKl5GTmJrA0m8tXeB_nsSssoLSDx5NDP-dHQmil5RmFEA9vzkepEvTimoLGapkidUqYTCqZBz9pJRNp-fLc7jdx_OqXjNZzDLVy9YzO5Fh9OP-0MtIBaS3TyMjkL4BgBKZXAQHWSKCkjVYbS6sNaUW-IsKdeuMWFrvGuI7iritmvjydgIxHVkvWt1R8LG-Jbo0rvgWt0Qb8LGdaH-YToTwqPogdVNMI_373H06fLiY_42Xq6uFvnZMi6FSkTMja2otoXMDEghbF8JISorbapFWlhZFCllGiqldJkoypRNKgVFBYZxXSl-HD0bczfe3e56bGzrUJqm0Z1xu4BpJpkAJfkEMAAHbyxufN1qf4cUcBCNOIjGQRsO2nAUjUIiw140Yi8a_4hGjoD5auj3uU_2ALuiNdWUujfbz5_u5zqUurFed2UdpjXOEkiZ6Nc-j2s_68bc_cX2H7R_kY2NPjgeg-v-gr-mYO2_Y5LyVOKX91d4zZZfk5vLN8j5b5s0taQ</recordid><startdate>199610</startdate><enddate>199610</enddate><creator>Cross, Nicholas L.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199610</creationdate><title>Effect of cholesterol and other sterols on human sperm acrosomal responsiveness</title><author>Cross, Nicholas L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4964-3efd1afb58e0544ffb5444df5f7a47bf5bb712a0d99ac69129f6d90bd0e23ad93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>acrosome</topic><topic>Acrosome - drug effects</topic><topic>acrosome reaction</topic><topic>Biological and medical sciences</topic><topic>capacitation</topic><topic>Cholesterol - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Morphology. Physiology</topic><topic>Sperm Capacitation - drug effects</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - physiology</topic><topic>Sterols - pharmacology</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cross, Nicholas L.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular reproduction and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cross, Nicholas L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of cholesterol and other sterols on human sperm acrosomal responsiveness</atitle><jtitle>Molecular reproduction and development</jtitle><addtitle>Mol. Reprod. Dev</addtitle><date>1996-10</date><risdate>1996</risdate><volume>45</volume><issue>2</issue><spage>212</spage><epage>217</epage><pages>212-217</pages><issn>1040-452X</issn><eissn>1098-2795</eissn><coden>MREDEE</coden><abstract>Human sperm become responsive to inducers of the acrosome reaction when they are washed free of seminal plasma and incubated in an appropriate medium. Previous work has shown that cholesterol‐enriched medium prevents sperm from becoming responsive to the inducer, progesterone. Sperm that were incubated 24 hr in cholesterol‐enriched medium and then treated with progesterone showed no evidence of membrane fusion, indicating that cholesterol acts at a stage before the earliest morphological change. Inhibition of acrosomal responsiveness by cholesterol was reversible. Among other sterols reported in mammalian sperm, desmosterol and cholesterol sulfate also inhibited sperm from becoming responsive, but cholesterol palmitate did not. Our results support a model in which sperm unesterified cholesterol, or a molecule in equilibrium with it, suppresses acrosomal responsiveness. Cholesterol‐enriched medium also prevented sperm from becoming responsive to the calcium/proton exchanging ionophore, ionomycin, suggesting that cholesterol's effect may be, at least in part, at a point in the signal transduction pathway subsequent to the rise in intracellular‐free calcium. © 1996 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>8914079</pmid><doi>10.1002/(SICI)1098-2795(199610)45:2<212::AID-MRD14>3.0.CO;2-2</doi><tpages>6</tpages></addata></record> |
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| ispartof | Molecular reproduction and development, 1996-10, Vol.45 (2), p.212-217 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | acrosome Acrosome - drug effects acrosome reaction Biological and medical sciences capacitation Cholesterol - pharmacology Fundamental and applied biological sciences. Psychology Humans Male Mammalian male genital system Morphology. Physiology Sperm Capacitation - drug effects Spermatozoa - drug effects Spermatozoa - physiology Sterols - pharmacology Vertebrates: reproduction |
| title | Effect of cholesterol and other sterols on human sperm acrosomal responsiveness |
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