Components of multiprotein-RNA complex that controls transcription elongation in Escherichia coli phage lambda

This chapter discusses the components of multiprotein-RNA complex that controls transcription elongation in Escherichia Coli phage λ. Studies of bacteriophage A led to the discovery of several basic mechanisms of transcriptional regulation. One of these is transcriptional antitermination, the proces...

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Veröffentlicht in:	Methods in Enzymology 1996, Vol.274, p.374-402
Hauptverfasser:	Das, Asis, Pal, Mahadeb, Mena, Jaime Garcia, Whalen, William, Wolska, Krystyna, Crossley, Robin, Rees, William, von Hippel, Peter H., Costantino, Nina, Court, Donald, Mazzulla, Marie, Altieri, Amanda S., Byrd, R.Andrew, Chattopadhyay, Samit, DeVito, Joseph, Ghosh, Balaram
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - metabolism Bacterial Proteins - isolation & purification Bacterial Proteins - metabolism Bacteriophage lambda - genetics Bacteriophage lambda - metabolism Base Sequence Chromatography, Affinity - methods Chromatography, Ion Exchange - methods DNA-Directed RNA Polymerases - metabolism Electrophoresis, Polyacrylamide Gel - methods Escherichia coli - genetics Escherichia coli - metabolism Escherichia coli Proteins Indicators and Reagents Kinetics Models, Genetic Molecular Sequence Data Nucleic Acid Conformation Nucleocapsid - isolation & purification Nucleocapsid - metabolism Operon Peptide Elongation Factors - isolation & purification Peptide Elongation Factors - metabolism Phosphorus Radioisotopes Promoter Regions, Genetic Radioisotope Dilution Technique Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism RNA, Viral - analysis RNA, Viral - biosynthesis RNA, Viral - chemistry Terminator Regions, Genetic Transcription Factors - isolation & purification Transcription Factors - metabolism Transcription, Genetic Transcriptional Elongation Factors
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