Components of multiprotein-RNA complex that controls transcription elongation in Escherichia coli phage lambda
This chapter discusses the components of multiprotein-RNA complex that controls transcription elongation in Escherichia Coli phage λ. Studies of bacteriophage A led to the discovery of several basic mechanisms of transcriptional regulation. One of these is transcriptional antitermination, the proces...
Gespeichert in:
| Veröffentlicht in: | Methods in Enzymology 1996, Vol.274, p.374-402 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 402 |
|---|---|
| container_issue | |
| container_start_page | 374 |
| container_title | Methods in Enzymology |
| container_volume | 274 |
| creator | Das, Asis Pal, Mahadeb Mena, Jaime Garcia Whalen, William Wolska, Krystyna Crossley, Robin Rees, William von Hippel, Peter H. Costantino, Nina Court, Donald Mazzulla, Marie Altieri, Amanda S. Byrd, R.Andrew Chattopadhyay, Samit DeVito, Joseph Ghosh, Balaram |
| description | This chapter discusses the components of multiprotein-RNA complex that controls transcription elongation in Escherichia Coli phage λ. Studies of bacteriophage A led to the discovery of several basic mechanisms of transcriptional regulation. One of these is transcriptional antitermination, the process in which genes whose transcription is otherwise blocked by premature termination are expressed through termination suppression. In λ and related phages, genome-specific antiterminators convert RNA polymerase (RNAP) into a termination-resistant form during early phases of transcription elongation. The chapter describes the current understanding of how one such antiterminator, the λ N gene product, works. The chapter reviews the methods of overproduction, isolation, and assay of the N protein as well as several accessory factors that modulate transcription elongation in Escherichia coli in the form of a multiprotein-RNA complex. The availability of N and Nus factors in large scale should now make it possible not only to attempt to determine the structures of the individual protein components, and the various RNA-protein and protein-protein complexes by biophysical methods, but also to examine these interactions by conventional biochemical methods. |
| doi_str_mv | 10.1016/S0076-6879(96)74032-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_78523211</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0076687996740326</els_id><sourcerecordid>78523211</sourcerecordid><originalsourceid>FETCH-LOGICAL-e264t-152d8ad787bdd94b6c99832ab1ccc16b29f6d2e13260373ea55c66e2a86d20953</originalsourceid><addsrcrecordid>eNo9UU1PGzEQtVpQmkB_QiSfqnJYsL1Zf5wqFEFbCYHUwtny2pPEldfe2k4F_54lRD3Nx3szmnkPoSUll5RQfvWbEMEbLoX6qviFWJGWNfwDmtOuE41QUn5EC0IZlYyKTpyg-X_-J7Qo5Q8hTEhFZ2gmFWGSkTmK6zSMKUKsBacNHvah-jGnCj42v-6vsZ3gAM-47kydilhzCgXXbGKx2Y_Vp4ghpLg1h9RHfFPsDrK3O2-mgeDxuDNbwMEMvTPn6HRjQoHPx3iGnm5vHtc_mruH7z_X13cNML6qDe2Yk8YJKXrn1KrnVinZMtNTay3lPVMb7hjQlnHSihZM11nOgRk5tYnq2jP05X3v9MvfPZSqB18shGAipH3RQnasZZROxOWRuO8HcHrMfjD5RR8FmvBv7zhM1_7zkHWxHqIF5zPYql3ymhL9Zo8-2KPf5NaK64M9mrevWyyA9g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>78523211</pqid></control><display><type>article</type><title>Components of multiprotein-RNA complex that controls transcription elongation in Escherichia coli phage lambda</title><source>MEDLINE</source><source>ScienceDirect eBooks</source><source>Access via ScienceDirect (Elsevier)</source><creator>Das, Asis ; Pal, Mahadeb ; Mena, Jaime Garcia ; Whalen, William ; Wolska, Krystyna ; Crossley, Robin ; Rees, William ; von Hippel, Peter H. ; Costantino, Nina ; Court, Donald ; Mazzulla, Marie ; Altieri, Amanda S. ; Byrd, R.Andrew ; Chattopadhyay, Samit ; DeVito, Joseph ; Ghosh, Balaram</creator><creatorcontrib>Das, Asis ; Pal, Mahadeb ; Mena, Jaime Garcia ; Whalen, William ; Wolska, Krystyna ; Crossley, Robin ; Rees, William ; von Hippel, Peter H. ; Costantino, Nina ; Court, Donald ; Mazzulla, Marie ; Altieri, Amanda S. ; Byrd, R.Andrew ; Chattopadhyay, Samit ; DeVito, Joseph ; Ghosh, Balaram</creatorcontrib><description>This chapter discusses the components of multiprotein-RNA complex that controls transcription elongation in Escherichia Coli phage λ. Studies of bacteriophage A led to the discovery of several basic mechanisms of transcriptional regulation. One of these is transcriptional antitermination, the process in which genes whose transcription is otherwise blocked by premature termination are expressed through termination suppression. In λ and related phages, genome-specific antiterminators convert RNA polymerase (RNAP) into a termination-resistant form during early phases of transcription elongation. The chapter describes the current understanding of how one such antiterminator, the λ N gene product, works. The chapter reviews the methods of overproduction, isolation, and assay of the N protein as well as several accessory factors that modulate transcription elongation in Escherichia coli in the form of a multiprotein-RNA complex. The availability of N and Nus factors in large scale should now make it possible not only to attempt to determine the structures of the individual protein components, and the various RNA-protein and protein-protein complexes by biophysical methods, but also to examine these interactions by conventional biochemical methods.</description><identifier>ISSN: 0076-6879</identifier><identifier>ISBN: 0121821757</identifier><identifier>ISBN: 9780121821753</identifier><identifier>EISSN: 1557-7988</identifier><identifier>DOI: 10.1016/S0076-6879(96)74032-6</identifier><identifier>PMID: 8902820</identifier><language>eng</language><publisher>United States: Elsevier Science & Technology</publisher><subject>Adenosine Triphosphate - metabolism ; Bacterial Proteins - isolation & purification ; Bacterial Proteins - metabolism ; Bacteriophage lambda - genetics ; Bacteriophage lambda - metabolism ; Base Sequence ; Chromatography, Affinity - methods ; Chromatography, Ion Exchange - methods ; DNA-Directed RNA Polymerases - metabolism ; Electrophoresis, Polyacrylamide Gel - methods ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Escherichia coli Proteins ; Indicators and Reagents ; Kinetics ; Models, Genetic ; Molecular Sequence Data ; Nucleic Acid Conformation ; Nucleocapsid - isolation & purification ; Nucleocapsid - metabolism ; Operon ; Peptide Elongation Factors - isolation & purification ; Peptide Elongation Factors - metabolism ; Phosphorus Radioisotopes ; Promoter Regions, Genetic ; Radioisotope Dilution Technique ; Recombinant Proteins - isolation & purification ; Recombinant Proteins - metabolism ; RNA, Viral - analysis ; RNA, Viral - biosynthesis ; RNA, Viral - chemistry ; Terminator Regions, Genetic ; Transcription Factors - isolation & purification ; Transcription Factors - metabolism ; Transcription, Genetic ; Transcriptional Elongation Factors</subject><ispartof>Methods in Enzymology, 1996, Vol.274, p.374-402</ispartof><rights>1996</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0076-6879(96)74032-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,779,780,784,793,3459,3550,4024,11288,27923,27924,27925,45810,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8902820$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Das, Asis</creatorcontrib><creatorcontrib>Pal, Mahadeb</creatorcontrib><creatorcontrib>Mena, Jaime Garcia</creatorcontrib><creatorcontrib>Whalen, William</creatorcontrib><creatorcontrib>Wolska, Krystyna</creatorcontrib><creatorcontrib>Crossley, Robin</creatorcontrib><creatorcontrib>Rees, William</creatorcontrib><creatorcontrib>von Hippel, Peter H.</creatorcontrib><creatorcontrib>Costantino, Nina</creatorcontrib><creatorcontrib>Court, Donald</creatorcontrib><creatorcontrib>Mazzulla, Marie</creatorcontrib><creatorcontrib>Altieri, Amanda S.</creatorcontrib><creatorcontrib>Byrd, R.Andrew</creatorcontrib><creatorcontrib>Chattopadhyay, Samit</creatorcontrib><creatorcontrib>DeVito, Joseph</creatorcontrib><creatorcontrib>Ghosh, Balaram</creatorcontrib><title>Components of multiprotein-RNA complex that controls transcription elongation in Escherichia coli phage lambda</title><title>Methods in Enzymology</title><addtitle>Methods Enzymol</addtitle><description>This chapter discusses the components of multiprotein-RNA complex that controls transcription elongation in Escherichia Coli phage λ. Studies of bacteriophage A led to the discovery of several basic mechanisms of transcriptional regulation. One of these is transcriptional antitermination, the process in which genes whose transcription is otherwise blocked by premature termination are expressed through termination suppression. In λ and related phages, genome-specific antiterminators convert RNA polymerase (RNAP) into a termination-resistant form during early phases of transcription elongation. The chapter describes the current understanding of how one such antiterminator, the λ N gene product, works. The chapter reviews the methods of overproduction, isolation, and assay of the N protein as well as several accessory factors that modulate transcription elongation in Escherichia coli in the form of a multiprotein-RNA complex. The availability of N and Nus factors in large scale should now make it possible not only to attempt to determine the structures of the individual protein components, and the various RNA-protein and protein-protein complexes by biophysical methods, but also to examine these interactions by conventional biochemical methods.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Bacterial Proteins - isolation & purification</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriophage lambda - genetics</subject><subject>Bacteriophage lambda - metabolism</subject><subject>Base Sequence</subject><subject>Chromatography, Affinity - methods</subject><subject>Chromatography, Ion Exchange - methods</subject><subject>DNA-Directed RNA Polymerases - metabolism</subject><subject>Electrophoresis, Polyacrylamide Gel - methods</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Escherichia coli Proteins</subject><subject>Indicators and Reagents</subject><subject>Kinetics</subject><subject>Models, Genetic</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Conformation</subject><subject>Nucleocapsid - isolation & purification</subject><subject>Nucleocapsid - metabolism</subject><subject>Operon</subject><subject>Peptide Elongation Factors - isolation & purification</subject><subject>Peptide Elongation Factors - metabolism</subject><subject>Phosphorus Radioisotopes</subject><subject>Promoter Regions, Genetic</subject><subject>Radioisotope Dilution Technique</subject><subject>Recombinant Proteins - isolation & purification</subject><subject>Recombinant Proteins - metabolism</subject><subject>RNA, Viral - analysis</subject><subject>RNA, Viral - biosynthesis</subject><subject>RNA, Viral - chemistry</subject><subject>Terminator Regions, Genetic</subject><subject>Transcription Factors - isolation & purification</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription, Genetic</subject><subject>Transcriptional Elongation Factors</subject><issn>0076-6879</issn><issn>1557-7988</issn><isbn>0121821757</isbn><isbn>9780121821753</isbn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UU1PGzEQtVpQmkB_QiSfqnJYsL1Zf5wqFEFbCYHUwtny2pPEldfe2k4F_54lRD3Nx3szmnkPoSUll5RQfvWbEMEbLoX6qviFWJGWNfwDmtOuE41QUn5EC0IZlYyKTpyg-X_-J7Qo5Q8hTEhFZ2gmFWGSkTmK6zSMKUKsBacNHvah-jGnCj42v-6vsZ3gAM-47kydilhzCgXXbGKx2Y_Vp4ghpLg1h9RHfFPsDrK3O2-mgeDxuDNbwMEMvTPn6HRjQoHPx3iGnm5vHtc_mruH7z_X13cNML6qDe2Yk8YJKXrn1KrnVinZMtNTay3lPVMb7hjQlnHSihZM11nOgRk5tYnq2jP05X3v9MvfPZSqB18shGAipH3RQnasZZROxOWRuO8HcHrMfjD5RR8FmvBv7zhM1_7zkHWxHqIF5zPYql3ymhL9Zo8-2KPf5NaK64M9mrevWyyA9g</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>Das, Asis</creator><creator>Pal, Mahadeb</creator><creator>Mena, Jaime Garcia</creator><creator>Whalen, William</creator><creator>Wolska, Krystyna</creator><creator>Crossley, Robin</creator><creator>Rees, William</creator><creator>von Hippel, Peter H.</creator><creator>Costantino, Nina</creator><creator>Court, Donald</creator><creator>Mazzulla, Marie</creator><creator>Altieri, Amanda S.</creator><creator>Byrd, R.Andrew</creator><creator>Chattopadhyay, Samit</creator><creator>DeVito, Joseph</creator><creator>Ghosh, Balaram</creator><general>Elsevier Science & Technology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>1996</creationdate><title>Components of multiprotein-RNA complex that controls transcription elongation in Escherichia coli phage lambda</title><author>Das, Asis ; Pal, Mahadeb ; Mena, Jaime Garcia ; Whalen, William ; Wolska, Krystyna ; Crossley, Robin ; Rees, William ; von Hippel, Peter H. ; Costantino, Nina ; Court, Donald ; Mazzulla, Marie ; Altieri, Amanda S. ; Byrd, R.Andrew ; Chattopadhyay, Samit ; DeVito, Joseph ; Ghosh, Balaram</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e264t-152d8ad787bdd94b6c99832ab1ccc16b29f6d2e13260373ea55c66e2a86d20953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Bacterial Proteins - isolation & purification</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriophage lambda - genetics</topic><topic>Bacteriophage lambda - metabolism</topic><topic>Base Sequence</topic><topic>Chromatography, Affinity - methods</topic><topic>Chromatography, Ion Exchange - methods</topic><topic>DNA-Directed RNA Polymerases - metabolism</topic><topic>Electrophoresis, Polyacrylamide Gel - methods</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>Escherichia coli Proteins</topic><topic>Indicators and Reagents</topic><topic>Kinetics</topic><topic>Models, Genetic</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Conformation</topic><topic>Nucleocapsid - isolation & purification</topic><topic>Nucleocapsid - metabolism</topic><topic>Operon</topic><topic>Peptide Elongation Factors - isolation & purification</topic><topic>Peptide Elongation Factors - metabolism</topic><topic>Phosphorus Radioisotopes</topic><topic>Promoter Regions, Genetic</topic><topic>Radioisotope Dilution Technique</topic><topic>Recombinant Proteins - isolation & purification</topic><topic>Recombinant Proteins - metabolism</topic><topic>RNA, Viral - analysis</topic><topic>RNA, Viral - biosynthesis</topic><topic>RNA, Viral - chemistry</topic><topic>Terminator Regions, Genetic</topic><topic>Transcription Factors - isolation & purification</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription, Genetic</topic><topic>Transcriptional Elongation Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Das, Asis</creatorcontrib><creatorcontrib>Pal, Mahadeb</creatorcontrib><creatorcontrib>Mena, Jaime Garcia</creatorcontrib><creatorcontrib>Whalen, William</creatorcontrib><creatorcontrib>Wolska, Krystyna</creatorcontrib><creatorcontrib>Crossley, Robin</creatorcontrib><creatorcontrib>Rees, William</creatorcontrib><creatorcontrib>von Hippel, Peter H.</creatorcontrib><creatorcontrib>Costantino, Nina</creatorcontrib><creatorcontrib>Court, Donald</creatorcontrib><creatorcontrib>Mazzulla, Marie</creatorcontrib><creatorcontrib>Altieri, Amanda S.</creatorcontrib><creatorcontrib>Byrd, R.Andrew</creatorcontrib><creatorcontrib>Chattopadhyay, Samit</creatorcontrib><creatorcontrib>DeVito, Joseph</creatorcontrib><creatorcontrib>Ghosh, Balaram</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Methods in Enzymology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Das, Asis</au><au>Pal, Mahadeb</au><au>Mena, Jaime Garcia</au><au>Whalen, William</au><au>Wolska, Krystyna</au><au>Crossley, Robin</au><au>Rees, William</au><au>von Hippel, Peter H.</au><au>Costantino, Nina</au><au>Court, Donald</au><au>Mazzulla, Marie</au><au>Altieri, Amanda S.</au><au>Byrd, R.Andrew</au><au>Chattopadhyay, Samit</au><au>DeVito, Joseph</au><au>Ghosh, Balaram</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Components of multiprotein-RNA complex that controls transcription elongation in Escherichia coli phage lambda</atitle><jtitle>Methods in Enzymology</jtitle><addtitle>Methods Enzymol</addtitle><date>1996</date><risdate>1996</risdate><volume>274</volume><spage>374</spage><epage>402</epage><pages>374-402</pages><issn>0076-6879</issn><eissn>1557-7988</eissn><isbn>0121821757</isbn><isbn>9780121821753</isbn><abstract>This chapter discusses the components of multiprotein-RNA complex that controls transcription elongation in Escherichia Coli phage λ. Studies of bacteriophage A led to the discovery of several basic mechanisms of transcriptional regulation. One of these is transcriptional antitermination, the process in which genes whose transcription is otherwise blocked by premature termination are expressed through termination suppression. In λ and related phages, genome-specific antiterminators convert RNA polymerase (RNAP) into a termination-resistant form during early phases of transcription elongation. The chapter describes the current understanding of how one such antiterminator, the λ N gene product, works. The chapter reviews the methods of overproduction, isolation, and assay of the N protein as well as several accessory factors that modulate transcription elongation in Escherichia coli in the form of a multiprotein-RNA complex. The availability of N and Nus factors in large scale should now make it possible not only to attempt to determine the structures of the individual protein components, and the various RNA-protein and protein-protein complexes by biophysical methods, but also to examine these interactions by conventional biochemical methods.</abstract><cop>United States</cop><pub>Elsevier Science & Technology</pub><pmid>8902820</pmid><doi>10.1016/S0076-6879(96)74032-6</doi><tpages>29</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0076-6879 |
| ispartof | Methods in Enzymology, 1996, Vol.274, p.374-402 |
| issn | 0076-6879 1557-7988 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78523211 |
| source | MEDLINE; ScienceDirect eBooks; Access via ScienceDirect (Elsevier) |
| subjects | Adenosine Triphosphate - metabolism Bacterial Proteins - isolation & purification Bacterial Proteins - metabolism Bacteriophage lambda - genetics Bacteriophage lambda - metabolism Base Sequence Chromatography, Affinity - methods Chromatography, Ion Exchange - methods DNA-Directed RNA Polymerases - metabolism Electrophoresis, Polyacrylamide Gel - methods Escherichia coli - genetics Escherichia coli - metabolism Escherichia coli Proteins Indicators and Reagents Kinetics Models, Genetic Molecular Sequence Data Nucleic Acid Conformation Nucleocapsid - isolation & purification Nucleocapsid - metabolism Operon Peptide Elongation Factors - isolation & purification Peptide Elongation Factors - metabolism Phosphorus Radioisotopes Promoter Regions, Genetic Radioisotope Dilution Technique Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism RNA, Viral - analysis RNA, Viral - biosynthesis RNA, Viral - chemistry Terminator Regions, Genetic Transcription Factors - isolation & purification Transcription Factors - metabolism Transcription, Genetic Transcriptional Elongation Factors |
| title | Components of multiprotein-RNA complex that controls transcription elongation in Escherichia coli phage lambda |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T12%3A38%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Components%20of%20multiprotein-RNA%20complex%20that%20controls%20transcription%20elongation%20in%20Escherichia%20coli%20phage%20lambda&rft.jtitle=Methods%20in%20Enzymology&rft.au=Das,%20Asis&rft.date=1996&rft.volume=274&rft.spage=374&rft.epage=402&rft.pages=374-402&rft.issn=0076-6879&rft.eissn=1557-7988&rft.isbn=0121821757&rft.isbn_list=9780121821753&rft_id=info:doi/10.1016/S0076-6879(96)74032-6&rft_dat=%3Cproquest_pubme%3E78523211%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=78523211&rft_id=info:pmid/8902820&rft_els_id=S0076687996740326&rfr_iscdi=true |