An Epitope Chimeric Antigen for the Hepatitis C Virus Serological Screening Test
The epitope chimeric antigen, CepCM, composed of the 9 selected major epitope regions (two in NS3, two each in the NS4 of two genotypes, two each in the core of two genotype, and one in the core in hepatitis C virus (HCV) polypeptide), was expressed as a fusion protein of the trpE peptide in E. coli...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 1996/10/15, Vol.19(10), pp.1254-1260 |
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| container_title | Biological & pharmaceutical bulletin |
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| creator | YAGI, Shintaro KASHIWAKUMA, Tomiko YAMAGUCHI, Kenjiro CHIBA, Yukie OHTSUKA, Eiko HASEGAWA, Akira |
| description | The epitope chimeric antigen, CepCM, composed of the 9 selected major epitope regions (two in NS3, two each in the NS4 of two genotypes, two each in the core of two genotype, and one in the core in hepatitis C virus (HCV) polypeptide), was expressed as a fusion protein of the trpE peptide in E. coli. An ELISA test using this antigen produced the same judgements with most of the panel sera as a second generation HCV screening kit. Though discrepancies were found in twelve samples (5% of the samples), further analysis revealed that eleven samples were indeterminate sera as judged by an immunoblot test. The reactivity found in several seroconversion series sera suggested that CepCM has superior reactivity to HCV infected sera than some second generation kits. These data indicated that an epitope chimeric antigen with a man-made sequence will be a excellent tool for a diagnostic test kit. |
| doi_str_mv | 10.1248/bpb.19.1254 |
| format | Article |
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An ELISA test using this antigen produced the same judgements with most of the panel sera as a second generation HCV screening kit. Though discrepancies were found in twelve samples (5% of the samples), further analysis revealed that eleven samples were indeterminate sera as judged by an immunoblot test. The reactivity found in several seroconversion series sera suggested that CepCM has superior reactivity to HCV infected sera than some second generation kits. These data indicated that an epitope chimeric antigen with a man-made sequence will be a excellent tool for a diagnostic test kit.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.19.1254</identifier><identifier>PMID: 8913492</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>Antigens, Viral - chemistry ; Antigens, Viral - immunology ; Blotting, Western ; Enzyme-Linked Immunosorbent Assay - methods ; epitope chimeric antigen ; Epitopes - analysis ; Hepacivirus - genetics ; Hepatitis Antigens ; Hepatitis C ; Hepatitis C Antigens - analysis ; hepatitis C virus ; Humans ; man-made antigen ; Reagent Kits, Diagnostic ; Recombinant Fusion Proteins - analysis ; Recombinant Fusion Proteins - chemistry ; Recombinant Fusion Proteins - immunology ; serological screening</subject><ispartof>Biological and Pharmaceutical Bulletin, 1996/10/15, Vol.19(10), pp.1254-1260</ispartof><rights>The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 1996</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c610t-1a05d3bc5362b61f9c24a35797e9a63d59556748b745309829ec2786591c50733</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1881,27915,27916</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8913492$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YAGI, Shintaro</creatorcontrib><creatorcontrib>KASHIWAKUMA, Tomiko</creatorcontrib><creatorcontrib>YAMAGUCHI, Kenjiro</creatorcontrib><creatorcontrib>CHIBA, Yukie</creatorcontrib><creatorcontrib>OHTSUKA, Eiko</creatorcontrib><creatorcontrib>HASEGAWA, Akira</creatorcontrib><title>An Epitope Chimeric Antigen for the Hepatitis C Virus Serological Screening Test</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>The epitope chimeric antigen, CepCM, composed of the 9 selected major epitope regions (two in NS3, two each in the NS4 of two genotypes, two each in the core of two genotype, and one in the core in hepatitis C virus (HCV) polypeptide), was expressed as a fusion protein of the trpE peptide in E. coli. An ELISA test using this antigen produced the same judgements with most of the panel sera as a second generation HCV screening kit. Though discrepancies were found in twelve samples (5% of the samples), further analysis revealed that eleven samples were indeterminate sera as judged by an immunoblot test. The reactivity found in several seroconversion series sera suggested that CepCM has superior reactivity to HCV infected sera than some second generation kits. These data indicated that an epitope chimeric antigen with a man-made sequence will be a excellent tool for a diagnostic test kit.</description><subject>Antigens, Viral - chemistry</subject><subject>Antigens, Viral - immunology</subject><subject>Blotting, Western</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>epitope chimeric antigen</subject><subject>Epitopes - analysis</subject><subject>Hepacivirus - genetics</subject><subject>Hepatitis Antigens</subject><subject>Hepatitis C</subject><subject>Hepatitis C Antigens - analysis</subject><subject>hepatitis C virus</subject><subject>Humans</subject><subject>man-made antigen</subject><subject>Reagent Kits, Diagnostic</subject><subject>Recombinant Fusion Proteins - analysis</subject><subject>Recombinant Fusion Proteins - chemistry</subject><subject>Recombinant Fusion Proteins - immunology</subject><subject>serological screening</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtrGzEURkVJSJ20q6wDgkA3YVI9Rq-lMXlBIIGk3QqNfG3LjGemkmaRf185drzIJhtJ8B2OuPdD6JySa8pq_bsZmmtqylvU39CE8lpVglFxhCbEUF1JKvR3dJrSmhCiCOMn6ESbghk2Qc_TDt8MIfcD4NkqbCAGj6ddDkvo8KKPOK8A38Pgcsgh4Rn-G-KY8AvEvu2XwbsWv_gI0IVuiV8h5R_oeOHaBD_39xn6c3vzOruvHp_uHmbTx8pLSnJFHRFz3njBJWskXRjPaseFMgqMk3wujBBS1bpRteDEaGbAM6WlMNQLojg_Q7923iH2_8bysd2E5KFtXQf9mKzSZQdEsi9BKpTS5h28_ASu-zF2ZQhL69pQyYU0hbraUT72KUVY2CGGjYtvlhK7rcOWOiw1dltHoS_2zrHZwPzA7vdf8ttdvk7ZLeGQu5iDb2Hrosbwdx_5OIv4APiVixY6_h9ilZp6</recordid><startdate>19961001</startdate><enddate>19961001</enddate><creator>YAGI, Shintaro</creator><creator>KASHIWAKUMA, Tomiko</creator><creator>YAMAGUCHI, Kenjiro</creator><creator>CHIBA, Yukie</creator><creator>OHTSUKA, Eiko</creator><creator>HASEGAWA, Akira</creator><general>The Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19961001</creationdate><title>An Epitope Chimeric Antigen for the Hepatitis C Virus Serological Screening Test</title><author>YAGI, Shintaro ; KASHIWAKUMA, Tomiko ; YAMAGUCHI, Kenjiro ; CHIBA, Yukie ; OHTSUKA, Eiko ; HASEGAWA, Akira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c610t-1a05d3bc5362b61f9c24a35797e9a63d59556748b745309829ec2786591c50733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Antigens, Viral - chemistry</topic><topic>Antigens, Viral - immunology</topic><topic>Blotting, Western</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>epitope chimeric antigen</topic><topic>Epitopes - analysis</topic><topic>Hepacivirus - genetics</topic><topic>Hepatitis Antigens</topic><topic>Hepatitis C</topic><topic>Hepatitis C Antigens - analysis</topic><topic>hepatitis C virus</topic><topic>Humans</topic><topic>man-made antigen</topic><topic>Reagent Kits, Diagnostic</topic><topic>Recombinant Fusion Proteins - analysis</topic><topic>Recombinant Fusion Proteins - chemistry</topic><topic>Recombinant Fusion Proteins - immunology</topic><topic>serological screening</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YAGI, Shintaro</creatorcontrib><creatorcontrib>KASHIWAKUMA, Tomiko</creatorcontrib><creatorcontrib>YAMAGUCHI, Kenjiro</creatorcontrib><creatorcontrib>CHIBA, Yukie</creatorcontrib><creatorcontrib>OHTSUKA, Eiko</creatorcontrib><creatorcontrib>HASEGAWA, Akira</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YAGI, Shintaro</au><au>KASHIWAKUMA, Tomiko</au><au>YAMAGUCHI, Kenjiro</au><au>CHIBA, Yukie</au><au>OHTSUKA, Eiko</au><au>HASEGAWA, Akira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An Epitope Chimeric Antigen for the Hepatitis C Virus Serological Screening Test</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>1996-10-01</date><risdate>1996</risdate><volume>19</volume><issue>10</issue><spage>1254</spage><epage>1260</epage><pages>1254-1260</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>The epitope chimeric antigen, CepCM, composed of the 9 selected major epitope regions (two in NS3, two each in the NS4 of two genotypes, two each in the core of two genotype, and one in the core in hepatitis C virus (HCV) polypeptide), was expressed as a fusion protein of the trpE peptide in E. coli. An ELISA test using this antigen produced the same judgements with most of the panel sera as a second generation HCV screening kit. Though discrepancies were found in twelve samples (5% of the samples), further analysis revealed that eleven samples were indeterminate sera as judged by an immunoblot test. The reactivity found in several seroconversion series sera suggested that CepCM has superior reactivity to HCV infected sera than some second generation kits. These data indicated that an epitope chimeric antigen with a man-made sequence will be a excellent tool for a diagnostic test kit.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>8913492</pmid><doi>10.1248/bpb.19.1254</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biological and Pharmaceutical Bulletin, 1996/10/15, Vol.19(10), pp.1254-1260 |
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| source | J-STAGE Free; MEDLINE; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Antigens, Viral - chemistry Antigens, Viral - immunology Blotting, Western Enzyme-Linked Immunosorbent Assay - methods epitope chimeric antigen Epitopes - analysis Hepacivirus - genetics Hepatitis Antigens Hepatitis C Hepatitis C Antigens - analysis hepatitis C virus Humans man-made antigen Reagent Kits, Diagnostic Recombinant Fusion Proteins - analysis Recombinant Fusion Proteins - chemistry Recombinant Fusion Proteins - immunology serological screening |
| title | An Epitope Chimeric Antigen for the Hepatitis C Virus Serological Screening Test |
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