Purification of the Saccharomyces cerevisiae cleavage/polyadenylation factor I: separation into two components that are required for both cleavage and polyadenylation of mRNA 3' ends
The cleavage/polyadenylation factor I (CF I) is one of four factors required for mRNA 3' end formation in the yeast Saccharomyces cerevisiae. Here we describe the purification of CF I and its separation into two components, CF IA and CF IB. Both components are needed to reconstitute CF I activi...
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| Veröffentlicht in: | The Journal of biological chemistry 1996-10, Vol.271 (43), p.27167-27175 |
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| container_end_page | 27175 |
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| container_issue | 43 |
| container_start_page | 27167 |
| container_title | The Journal of biological chemistry |
| container_volume | 271 |
| creator | Kessler, M.M. (Tufts University School of Medicine, Boston, MA.) Zhao, J Moore, C.L |
| description | The cleavage/polyadenylation factor I (CF I) is one of four factors required for mRNA 3' end formation in the yeast Saccharomyces cerevisiae. Here we describe the purification of CF I and its separation into two components, CF IA and CF IB. Both components are needed to reconstitute CF I activity in cleavage and poly(A) addition. CF IA consists of a complex of four polypeptides of 76, 70, 50, and 38 kDa, and CF IB is a single 73-kDa polypeptide. The 76- and 38-kDa subunits of CF IA correspond to the previously identified RNA14 and RNA15 proteins. The RNA14 protein, but not the 70- or 50-kDa proteins, coimmunoprecipitates with the RNA15 protein, indicating that RNA14 and RNA15 proteins exist in a tight complex. RNA15 is the only subunit of CF I that can be cross-linked to pre-mRNA |
| doi_str_mv | 10.1074/jbc.271.43.27167 |
| format | Article |
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CF IA consists of a complex of four polypeptides of 76, 70, 50, and 38 kDa, and CF IB is a single 73-kDa polypeptide. The 76- and 38-kDa subunits of CF IA correspond to the previously identified RNA14 and RNA15 proteins. The RNA14 protein, but not the 70- or 50-kDa proteins, coimmunoprecipitates with the RNA15 protein, indicating that RNA14 and RNA15 proteins exist in a tight complex. RNA15 is the only subunit of CF I that can be cross-linked to pre-mRNA</abstract><cop>United States</cop><pmid>8900210</pmid><doi>10.1074/jbc.271.43.27167</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1996-10, Vol.271 (43), p.27167-27175 |
| issn | 0021-9258 1083-351X |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | ADENOSINA ADENOSINE ARN MENSAJERO ARN MESSAGER Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel Fungal Proteins - isolation & purification Fungal Proteins - metabolism mRNA Cleavage and Polyadenylation Factors Nuclear Proteins - metabolism PEPTIDE PEPTIDOS Poly A - metabolism PURIFICACION PURIFICATION RNA, Fungal - metabolism RNA-Binding Proteins - isolation & purification RNA-Binding Proteins - metabolism SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins |
| title | Purification of the Saccharomyces cerevisiae cleavage/polyadenylation factor I: separation into two components that are required for both cleavage and polyadenylation of mRNA 3' ends |
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