Demonstration of Antigenic and Genotypic Variation in Orientia tsutsugamushi Which Were Isolated in Japan, and Their Classification into Type and Subtype
A total of 40 strains of Orientia tsutsugamushi (34 isolates from patients and trombiculid mites in Japan, and 6 prototype strains of antigenic variants) were examined for classification based on the reactivities with type-specific monoclonal antibodies in indirect immunofluorescence tests, and on t...
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| Veröffentlicht in: | MICROBIOLOGY and IMMUNOLOGY 1996/09/20, Vol.40(9), pp.627-638 |
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| container_title | MICROBIOLOGY and IMMUNOLOGY |
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| creator | Ohashi, Norio Koyama, Yoko Urakami, Hiroshi Fukuhara, Masahiro Tamura, Akira Kawamori, Fumihiko Yamamoto, Seigo Kasuya, Shiro Yoshimura, Kentaro |
| description | A total of 40 strains of Orientia tsutsugamushi (34 isolates from patients and trombiculid mites in Japan, and 6 prototype strains of antigenic variants) were examined for classification based on the reactivities with type-specific monoclonal antibodies in indirect immunofluorescence tests, and on the restriction fragment length polymorphism of a polymerase chain reaction (PCR) -amplified 56-kilodalton type-specific antigenic protein gene. By these methods, several antigenic and genotypic variants were found among the strains, and these variants were classified into types and further into subtypes. These results suggest that there are many variants in O. tsutsugamushi, and the methods used here seem to be useful for the systematic classification of the numerous variants. A strain which may be a new type distinguishable from those identified previously was also found in this study. Furthermore, variety in the degree of pathogenicity in mice related to type and/or subtype classification were observed. |
| doi_str_mv | 10.1111/j.1348-0421.1996.tb01120.x |
| format | Article |
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By these methods, several antigenic and genotypic variants were found among the strains, and these variants were classified into types and further into subtypes. These results suggest that there are many variants in O. tsutsugamushi, and the methods used here seem to be useful for the systematic classification of the numerous variants. A strain which may be a new type distinguishable from those identified previously was also found in this study. Furthermore, variety in the degree of pathogenicity in mice related to type and/or subtype classification were observed.</description><identifier>ISSN: 0385-5600</identifier><identifier>EISSN: 1348-0421</identifier><identifier>DOI: 10.1111/j.1348-0421.1996.tb01120.x</identifier><identifier>PMID: 8908607</identifier><identifier>CODEN: MIIMDV</identifier><language>eng</language><publisher>Tokyo: Blackwell Publishing Ltd</publisher><subject>Animals ; Antigens, Bacterial - genetics ; Antigens, Bacterial - immunology ; Bacteriology ; Biological and medical sciences ; Classification ; Female ; Fluorescent Antibody Technique, Indirect ; Fundamental and applied biological sciences. Psychology ; Genetic Variation ; Humans ; Japan - epidemiology ; Mice ; Mice, Inbred C3H ; Microbiology ; Mites ; Orientia tsutsugamushi ; Orientia tsutsugamushi - classification ; Orientia tsutsugamushi - genetics ; Orientia tsutsugamushi - immunology ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Scrub Typhus - epidemiology ; Scrub Typhus - genetics ; Scrub Typhus - immunology ; Subtype ; Systematics</subject><ispartof>MICROBIOLOGY and IMMUNOLOGY, 1996/09/20, Vol.40(9), pp.627-638</ispartof><rights>Center for Academic Publications Japan</rights><rights>owned by Center for Academic Publications Japan (Publisher)</rights><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6260-d604ff94e33be4626a265f27e6275d16f03b08d8dd090eafd576af52c124e3bf3</citedby><cites>FETCH-LOGICAL-c6260-d604ff94e33be4626a265f27e6275d16f03b08d8dd090eafd576af52c124e3bf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2480836$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8908607$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ohashi, Norio</creatorcontrib><creatorcontrib>Koyama, Yoko</creatorcontrib><creatorcontrib>Urakami, Hiroshi</creatorcontrib><creatorcontrib>Fukuhara, Masahiro</creatorcontrib><creatorcontrib>Tamura, Akira</creatorcontrib><creatorcontrib>Kawamori, Fumihiko</creatorcontrib><creatorcontrib>Yamamoto, Seigo</creatorcontrib><creatorcontrib>Kasuya, Shiro</creatorcontrib><creatorcontrib>Yoshimura, Kentaro</creatorcontrib><title>Demonstration of Antigenic and Genotypic Variation in Orientia tsutsugamushi Which Were Isolated in Japan, and Their Classification into Type and Subtype</title><title>MICROBIOLOGY and IMMUNOLOGY</title><addtitle>Microbiology and Immunology</addtitle><description>A total of 40 strains of Orientia tsutsugamushi (34 isolates from patients and trombiculid mites in Japan, and 6 prototype strains of antigenic variants) were examined for classification based on the reactivities with type-specific monoclonal antibodies in indirect immunofluorescence tests, and on the restriction fragment length polymorphism of a polymerase chain reaction (PCR) -amplified 56-kilodalton type-specific antigenic protein gene. By these methods, several antigenic and genotypic variants were found among the strains, and these variants were classified into types and further into subtypes. These results suggest that there are many variants in O. tsutsugamushi, and the methods used here seem to be useful for the systematic classification of the numerous variants. A strain which may be a new type distinguishable from those identified previously was also found in this study. Furthermore, variety in the degree of pathogenicity in mice related to type and/or subtype classification were observed.</description><subject>Animals</subject><subject>Antigens, Bacterial - genetics</subject><subject>Antigens, Bacterial - immunology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Classification</subject><subject>Female</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Variation</subject><subject>Humans</subject><subject>Japan - epidemiology</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Microbiology</subject><subject>Mites</subject><subject>Orientia tsutsugamushi</subject><subject>Orientia tsutsugamushi - classification</subject><subject>Orientia tsutsugamushi - genetics</subject><subject>Orientia tsutsugamushi - immunology</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Scrub Typhus - epidemiology</subject><subject>Scrub Typhus - genetics</subject><subject>Scrub Typhus - immunology</subject><subject>Subtype</subject><subject>Systematics</subject><issn>0385-5600</issn><issn>1348-0421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkc2O0zAURiMEGjoDj4BkIcSKFNtJbIcVozKUwpRZUCg7y0muW5f8FNvRtI_C2-I2pWKHsCI78T33OPIXRc8JHpMwXm_GJElFjFNKxiTP2dgXmBCKx7sH0ehcehiNcCKyOGMYP44undtgTDkV6UV0IXIsGOaj6Nc7aLrWeau86VrUaXTderOC1pRItRWaQtv5_TZ8fVPWDJBp0Z01EDiFvOvDs1JN79YGLdemXKMlWEAz19XKQ3WgP6qtal8dfYs1GIsmtXLOaFP-EfoOLfZbOCJf-iKcCE-iR1rVDp6e1qvo6_ubxeRDfHs3nU2ub-OSUYbjiuFU6zyFJCkgDVuKskxTDozyrCJM46TAohJVhXMMSlcZZ0pntCQ09BQ6uYpeDt6t7X724LxsjCuhrlULXe8kFxkRIkv-CZKM5SIhPIBvBrC0nXMWtNxa0yi7lwTLQ4ByIw8pyUNK8hCgPAUod6H52emUvmigOreeEgv1F6e6cqWqtVVtadwZo6nAImEBeztg96aG_X_8gJzP5sfXoLgZFBvn1QrODmW9KWuQTQjLkJxzmWKZD1O49HO9XCsroQ2eePAY52H3l-aHZDzhmVx-nkr2aS74Ivsu8-Q3d1_iDw</recordid><startdate>19960101</startdate><enddate>19960101</enddate><creator>Ohashi, Norio</creator><creator>Koyama, Yoko</creator><creator>Urakami, Hiroshi</creator><creator>Fukuhara, Masahiro</creator><creator>Tamura, Akira</creator><creator>Kawamori, Fumihiko</creator><creator>Yamamoto, Seigo</creator><creator>Kasuya, Shiro</creator><creator>Yoshimura, Kentaro</creator><general>Blackwell Publishing Ltd</general><general>Center For Academic Publications Japan</general><general>Center for Academic Publications Japan</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19960101</creationdate><title>Demonstration of Antigenic and Genotypic Variation in Orientia tsutsugamushi Which Were Isolated in Japan, and Their Classification into Type and Subtype</title><author>Ohashi, Norio ; Koyama, Yoko ; Urakami, Hiroshi ; Fukuhara, Masahiro ; Tamura, Akira ; Kawamori, Fumihiko ; Yamamoto, Seigo ; Kasuya, Shiro ; Yoshimura, Kentaro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6260-d604ff94e33be4626a265f27e6275d16f03b08d8dd090eafd576af52c124e3bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Antigens, Bacterial - genetics</topic><topic>Antigens, Bacterial - immunology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Classification</topic><topic>Female</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Variation</topic><topic>Humans</topic><topic>Japan - epidemiology</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Microbiology</topic><topic>Mites</topic><topic>Orientia tsutsugamushi</topic><topic>Orientia tsutsugamushi - classification</topic><topic>Orientia tsutsugamushi - genetics</topic><topic>Orientia tsutsugamushi - immunology</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Scrub Typhus - epidemiology</topic><topic>Scrub Typhus - genetics</topic><topic>Scrub Typhus - immunology</topic><topic>Subtype</topic><topic>Systematics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ohashi, Norio</creatorcontrib><creatorcontrib>Koyama, Yoko</creatorcontrib><creatorcontrib>Urakami, Hiroshi</creatorcontrib><creatorcontrib>Fukuhara, Masahiro</creatorcontrib><creatorcontrib>Tamura, Akira</creatorcontrib><creatorcontrib>Kawamori, Fumihiko</creatorcontrib><creatorcontrib>Yamamoto, Seigo</creatorcontrib><creatorcontrib>Kasuya, Shiro</creatorcontrib><creatorcontrib>Yoshimura, Kentaro</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ohashi, Norio</au><au>Koyama, Yoko</au><au>Urakami, Hiroshi</au><au>Fukuhara, Masahiro</au><au>Tamura, Akira</au><au>Kawamori, Fumihiko</au><au>Yamamoto, Seigo</au><au>Kasuya, Shiro</au><au>Yoshimura, Kentaro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Demonstration of Antigenic and Genotypic Variation in Orientia tsutsugamushi Which Were Isolated in Japan, and Their Classification into Type and Subtype</atitle><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle><addtitle>Microbiology and Immunology</addtitle><date>1996-01-01</date><risdate>1996</risdate><volume>40</volume><issue>9</issue><spage>627</spage><epage>638</epage><pages>627-638</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><coden>MIIMDV</coden><abstract>A total of 40 strains of Orientia tsutsugamushi (34 isolates from patients and trombiculid mites in Japan, and 6 prototype strains of antigenic variants) were examined for classification based on the reactivities with type-specific monoclonal antibodies in indirect immunofluorescence tests, and on the restriction fragment length polymorphism of a polymerase chain reaction (PCR) -amplified 56-kilodalton type-specific antigenic protein gene. By these methods, several antigenic and genotypic variants were found among the strains, and these variants were classified into types and further into subtypes. These results suggest that there are many variants in O. tsutsugamushi, and the methods used here seem to be useful for the systematic classification of the numerous variants. A strain which may be a new type distinguishable from those identified previously was also found in this study. Furthermore, variety in the degree of pathogenicity in mice related to type and/or subtype classification were observed.</abstract><cop>Tokyo</cop><pub>Blackwell Publishing Ltd</pub><pmid>8908607</pmid><doi>10.1111/j.1348-0421.1996.tb01120.x</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0385-5600 |
| ispartof | MICROBIOLOGY and IMMUNOLOGY, 1996/09/20, Vol.40(9), pp.627-638 |
| issn | 0385-5600 1348-0421 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78518853 |
| source | MEDLINE; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Open Access Titles of Japan; Alma/SFX Local Collection |
| subjects | Animals Antigens, Bacterial - genetics Antigens, Bacterial - immunology Bacteriology Biological and medical sciences Classification Female Fluorescent Antibody Technique, Indirect Fundamental and applied biological sciences. Psychology Genetic Variation Humans Japan - epidemiology Mice Mice, Inbred C3H Microbiology Mites Orientia tsutsugamushi Orientia tsutsugamushi - classification Orientia tsutsugamushi - genetics Orientia tsutsugamushi - immunology Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Scrub Typhus - epidemiology Scrub Typhus - genetics Scrub Typhus - immunology Subtype Systematics |
| title | Demonstration of Antigenic and Genotypic Variation in Orientia tsutsugamushi Which Were Isolated in Japan, and Their Classification into Type and Subtype |
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