Direct Activation of the ATP-sensitive Potassium Channel by Oxygen Free Radicals in Guinea-pig Ventricular Cells:its Potentiation by MgADP
The effects of hydrogen peroxide (H 2O 2) on the activity of adenosine 5′-triphosphate-sensitive potassium (K + ATP) channels in ventricular cells isolated from guinea-pig hearts were investigated in inside-out membrane patches using the patch-clamp technique. H 2O 2at concentrations of 5 m mbut not...
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| Veröffentlicht in: | Journal of molecular and cellular cardiology 1996-09, Vol.28 (9), p.1867-1877 |
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| container_title | Journal of molecular and cellular cardiology |
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| creator | Ichinari, Koutarou Kakei, Masafumi Matsuoka, Tatsuru Nakashima, Hitoshi Tanaka, Hiromitsu |
| description | The effects of hydrogen peroxide (H
2O
2) on the activity of adenosine 5′-triphosphate-sensitive potassium (K
+
ATP) channels in ventricular cells isolated from guinea-pig hearts were investigated in inside-out membrane patches using the patch-clamp technique. H
2O
2at concentrations of 5 m
mbut not 1 m
mincreased K
+
ATPchannel activity in the presence of 0.3 m
mATP. The presence of 10
μ
mADP together with 0.3 m
mATP led to activation of the K
+
ATPchannel by 1 m
mH
2O
2. This potentiation of the H
2O
2-induced activation of the K
+
ATPchannel by ADP depended on the presence of Mg-ATP. Channel activation was due to an increase in the open-state probability and was not associated with a change in the single-channel conductance or the mean open and closed times during burst-like openings. The relationship between channel activity and ATP concentration could be fitted by the Hill equation with a Hill coefficient of 2 and the half maximal inhibition at 85
μ
mATP in the presence of 10
μ
mADP. The curve was shifted to higher ATP concentrations in a non-parallel manner by 1 m
mH
2O
2. Analysis of open-state probability for membrane patches containing several channels indicated that H
2O
2activated the individual channels to a differing extent. It is concluded that H
2O
2activates the K
+
ATPchannel directly by decreasing the sensitivity to ATP. This effect was potentiated by the presence of ADP. |
| doi_str_mv | 10.1006/jmcc.1996.0179 |
| format | Article |
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2O
2) on the activity of adenosine 5′-triphosphate-sensitive potassium (K
+
ATP) channels in ventricular cells isolated from guinea-pig hearts were investigated in inside-out membrane patches using the patch-clamp technique. H
2O
2at concentrations of 5 m
mbut not 1 m
mincreased K
+
ATPchannel activity in the presence of 0.3 m
mATP. The presence of 10
μ
mADP together with 0.3 m
mATP led to activation of the K
+
ATPchannel by 1 m
mH
2O
2. This potentiation of the H
2O
2-induced activation of the K
+
ATPchannel by ADP depended on the presence of Mg-ATP. Channel activation was due to an increase in the open-state probability and was not associated with a change in the single-channel conductance or the mean open and closed times during burst-like openings. The relationship between channel activity and ATP concentration could be fitted by the Hill equation with a Hill coefficient of 2 and the half maximal inhibition at 85
μ
mATP in the presence of 10
μ
mADP. The curve was shifted to higher ATP concentrations in a non-parallel manner by 1 m
mH
2O
2. Analysis of open-state probability for membrane patches containing several channels indicated that H
2O
2activated the individual channels to a differing extent. It is concluded that H
2O
2activates the K
+
ATPchannel directly by decreasing the sensitivity to ATP. This effect was potentiated by the presence of ADP.</description><identifier>ISSN: 0022-2828</identifier><identifier>EISSN: 1095-8584</identifier><identifier>DOI: 10.1006/jmcc.1996.0179</identifier><identifier>PMID: 8899545</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Adenosine Diphosphate - pharmacology ; Adenosine Triphosphate - pharmacology ; Animals ; ATP-sensitive K +channel ; Guinea Pigs ; Heart Ventricles - cytology ; Heart Ventricles - drug effects ; hydrogen peroxide ; Hydrogen Peroxide - pharmacology ; In Vitro Techniques ; Magnesium Chloride - pharmacology ; patch clamp ; Patch-Clamp Techniques ; Potassium Channels - drug effects ; Potassium Channels - physiology ; single ventricular myocytes ; Ventricular Function</subject><ispartof>Journal of molecular and cellular cardiology, 1996-09, Vol.28 (9), p.1867-1877</ispartof><rights>1996</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-ee8e4c8c5cbd866797bec618c91d493cba6bdb08a4eb4c187b9d2d5023d1f4183</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/jmcc.1996.0179$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8899545$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ichinari, Koutarou</creatorcontrib><creatorcontrib>Kakei, Masafumi</creatorcontrib><creatorcontrib>Matsuoka, Tatsuru</creatorcontrib><creatorcontrib>Nakashima, Hitoshi</creatorcontrib><creatorcontrib>Tanaka, Hiromitsu</creatorcontrib><title>Direct Activation of the ATP-sensitive Potassium Channel by Oxygen Free Radicals in Guinea-pig Ventricular Cells:its Potentiation by MgADP</title><title>Journal of molecular and cellular cardiology</title><addtitle>J Mol Cell Cardiol</addtitle><description>The effects of hydrogen peroxide (H
2O
2) on the activity of adenosine 5′-triphosphate-sensitive potassium (K
+
ATP) channels in ventricular cells isolated from guinea-pig hearts were investigated in inside-out membrane patches using the patch-clamp technique. H
2O
2at concentrations of 5 m
mbut not 1 m
mincreased K
+
ATPchannel activity in the presence of 0.3 m
mATP. The presence of 10
μ
mADP together with 0.3 m
mATP led to activation of the K
+
ATPchannel by 1 m
mH
2O
2. This potentiation of the H
2O
2-induced activation of the K
+
ATPchannel by ADP depended on the presence of Mg-ATP. Channel activation was due to an increase in the open-state probability and was not associated with a change in the single-channel conductance or the mean open and closed times during burst-like openings. The relationship between channel activity and ATP concentration could be fitted by the Hill equation with a Hill coefficient of 2 and the half maximal inhibition at 85
μ
mATP in the presence of 10
μ
mADP. The curve was shifted to higher ATP concentrations in a non-parallel manner by 1 m
mH
2O
2. Analysis of open-state probability for membrane patches containing several channels indicated that H
2O
2activated the individual channels to a differing extent. It is concluded that H
2O
2activates the K
+
ATPchannel directly by decreasing the sensitivity to ATP. This effect was potentiated by the presence of ADP.</description><subject>Adenosine Diphosphate - pharmacology</subject><subject>Adenosine Triphosphate - pharmacology</subject><subject>Animals</subject><subject>ATP-sensitive K +channel</subject><subject>Guinea Pigs</subject><subject>Heart Ventricles - cytology</subject><subject>Heart Ventricles - drug effects</subject><subject>hydrogen peroxide</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Magnesium Chloride - pharmacology</subject><subject>patch clamp</subject><subject>Patch-Clamp Techniques</subject><subject>Potassium Channels - drug effects</subject><subject>Potassium Channels - physiology</subject><subject>single ventricular myocytes</subject><subject>Ventricular Function</subject><issn>0022-2828</issn><issn>1095-8584</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUFv2yAYhtG0qcvaXXebxGk3Z2BjG3aL0rWb1KlR1e2K4ONLSmXjDHC1_IX96tlL1FtPSLwvDx8PhHzgbMkZaz4_9gBLrlSzZLxVr8iCM1UXspbiNVkwVpZFKUv5lrxL6ZExpkRVnZEzKZWqRb0gfy99RMh0Bdk_meyHQIctzQ9IV_ebImFIfgqQboZsUvJjT9cPJgTsqD3Q2z-HHQZ6FRHpnXEeTJeoD_R69AFNsfc7-gtDjh7GzkS6xq5LX3xOM23a98f7JtCP3epyc0HebCcAvj-t5-Tn1df79bfi5vb6-3p1U4BgdS4QJQqQUIN1smla1VqEhktQ3AlVgTWNdZZJI9AK4LK1ypWuZmXl-FZwWZ2TT0fuPg6_R0xZ9z7BNJsJOIxJt7LmvCrn4vJYhDikFHGr99H3Jh40Z3qWr2f5epavZ_nTgY8n8mh7dM_1k-0pl8ccp-c9eYw6gccA6P5_gnaDfwn9D5HplYM</recordid><startdate>19960901</startdate><enddate>19960901</enddate><creator>Ichinari, Koutarou</creator><creator>Kakei, Masafumi</creator><creator>Matsuoka, Tatsuru</creator><creator>Nakashima, Hitoshi</creator><creator>Tanaka, Hiromitsu</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960901</creationdate><title>Direct Activation of the ATP-sensitive Potassium Channel by Oxygen Free Radicals in Guinea-pig Ventricular Cells:its Potentiation by MgADP</title><author>Ichinari, Koutarou ; Kakei, Masafumi ; Matsuoka, Tatsuru ; Nakashima, Hitoshi ; Tanaka, Hiromitsu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-ee8e4c8c5cbd866797bec618c91d493cba6bdb08a4eb4c187b9d2d5023d1f4183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Adenosine Diphosphate - pharmacology</topic><topic>Adenosine Triphosphate - pharmacology</topic><topic>Animals</topic><topic>ATP-sensitive K +channel</topic><topic>Guinea Pigs</topic><topic>Heart Ventricles - cytology</topic><topic>Heart Ventricles - drug effects</topic><topic>hydrogen peroxide</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Magnesium Chloride - pharmacology</topic><topic>patch clamp</topic><topic>Patch-Clamp Techniques</topic><topic>Potassium Channels - drug effects</topic><topic>Potassium Channels - physiology</topic><topic>single ventricular myocytes</topic><topic>Ventricular Function</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ichinari, Koutarou</creatorcontrib><creatorcontrib>Kakei, Masafumi</creatorcontrib><creatorcontrib>Matsuoka, Tatsuru</creatorcontrib><creatorcontrib>Nakashima, Hitoshi</creatorcontrib><creatorcontrib>Tanaka, Hiromitsu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular and cellular cardiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ichinari, Koutarou</au><au>Kakei, Masafumi</au><au>Matsuoka, Tatsuru</au><au>Nakashima, Hitoshi</au><au>Tanaka, Hiromitsu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct Activation of the ATP-sensitive Potassium Channel by Oxygen Free Radicals in Guinea-pig Ventricular Cells:its Potentiation by MgADP</atitle><jtitle>Journal of molecular and cellular cardiology</jtitle><addtitle>J Mol Cell Cardiol</addtitle><date>1996-09-01</date><risdate>1996</risdate><volume>28</volume><issue>9</issue><spage>1867</spage><epage>1877</epage><pages>1867-1877</pages><issn>0022-2828</issn><eissn>1095-8584</eissn><abstract>The effects of hydrogen peroxide (H
2O
2) on the activity of adenosine 5′-triphosphate-sensitive potassium (K
+
ATP) channels in ventricular cells isolated from guinea-pig hearts were investigated in inside-out membrane patches using the patch-clamp technique. H
2O
2at concentrations of 5 m
mbut not 1 m
mincreased K
+
ATPchannel activity in the presence of 0.3 m
mATP. The presence of 10
μ
mADP together with 0.3 m
mATP led to activation of the K
+
ATPchannel by 1 m
mH
2O
2. This potentiation of the H
2O
2-induced activation of the K
+
ATPchannel by ADP depended on the presence of Mg-ATP. Channel activation was due to an increase in the open-state probability and was not associated with a change in the single-channel conductance or the mean open and closed times during burst-like openings. The relationship between channel activity and ATP concentration could be fitted by the Hill equation with a Hill coefficient of 2 and the half maximal inhibition at 85
μ
mATP in the presence of 10
μ
mADP. The curve was shifted to higher ATP concentrations in a non-parallel manner by 1 m
mH
2O
2. Analysis of open-state probability for membrane patches containing several channels indicated that H
2O
2activated the individual channels to a differing extent. It is concluded that H
2O
2activates the K
+
ATPchannel directly by decreasing the sensitivity to ATP. This effect was potentiated by the presence of ADP.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>8899545</pmid><doi>10.1006/jmcc.1996.0179</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-2828 |
| ispartof | Journal of molecular and cellular cardiology, 1996-09, Vol.28 (9), p.1867-1877 |
| issn | 0022-2828 1095-8584 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78511328 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adenosine Diphosphate - pharmacology Adenosine Triphosphate - pharmacology Animals ATP-sensitive K +channel Guinea Pigs Heart Ventricles - cytology Heart Ventricles - drug effects hydrogen peroxide Hydrogen Peroxide - pharmacology In Vitro Techniques Magnesium Chloride - pharmacology patch clamp Patch-Clamp Techniques Potassium Channels - drug effects Potassium Channels - physiology single ventricular myocytes Ventricular Function |
| title | Direct Activation of the ATP-sensitive Potassium Channel by Oxygen Free Radicals in Guinea-pig Ventricular Cells:its Potentiation by MgADP |
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