A Xenopus nodal-related gene that acts in synergy with noggin to induce complete secondary axis and notochord formation
Using a paracrine assay to screen for signaling proteins that could respecify ectodermal tissue, we isolated a Xenopus gene related to the mouse gene nodal, a member of the TGFbeta superfamily. The gene is expressed in three regions in the early Xenopus embryo: first in the gastrula organizer, then...
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| Veröffentlicht in: | Development (Cambridge) 1996-10, Vol.122 (10), p.3275-3282 |
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| container_title | Development (Cambridge) |
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| creator | Lustig, K D Kroll, K Sun, E Ramos, R Elmendorf, H Kirschner, M W |
| description | Using a paracrine assay to screen for signaling proteins that could respecify ectodermal tissue, we isolated a Xenopus gene related to the mouse gene nodal, a member of the TGFbeta superfamily. The gene is expressed in three regions in the early Xenopus embryo: first in the gastrula organizer, then in two stripes of cells flanking the posterior notochord in late neurulae, and finally in lateral plate mesoderm restricted to the left side of tailbud-stage embryos. Ectopic expression of the gene induces muscle formation in ectodermal explants and partial secondary axes in whole embryos. Together with noggin, another secreted protein also present in the organizer, it induces notochord formation in ectodermal explants and complete secondary axes in whole embryos. These results suggest that the nodal-related gene may act together with noggin to induce axial pattern during gastrulation and also may play a role in left-right asymmetry generation in the post-gastrula embryo. |
| doi_str_mv | 10.1242/dev.122.10.3275 |
| format | Article |
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Kroll, K ; Sun, E ; Ramos, R ; Elmendorf, H ; Kirschner, M W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-a7287a63cb2cc519a77ff9f12fc05b1de2dd1dec9c730c02174ab48beccea04f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Axis, Cervical Vertebra - embryology</topic><topic>Axis, Cervical Vertebra - physiology</topic><topic>Carrier Proteins</topic><topic>Cloning, Molecular</topic><topic>Ectoderm - physiology</topic><topic>Endoderm - physiology</topic><topic>Freshwater</topic><topic>Gastrula - physiology</topic><topic>Gene Expression</topic><topic>Nodal Protein</topic><topic>Notochord - embryology</topic><topic>Notochord - physiology</topic><topic>Proteins</topic><topic>Transforming Growth Factor beta - genetics</topic><topic>Transforming Growth Factor beta - metabolism</topic><topic>Xenopus laevis</topic><topic>Xenopus laevis - embryology</topic><topic>Xenopus laevis - metabolism</topic><topic>Xenopus Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lustig, K D</creatorcontrib><creatorcontrib>Kroll, K</creatorcontrib><creatorcontrib>Sun, E</creatorcontrib><creatorcontrib>Ramos, R</creatorcontrib><creatorcontrib>Elmendorf, H</creatorcontrib><creatorcontrib>Kirschner, M W</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Development (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lustig, K D</au><au>Kroll, K</au><au>Sun, E</au><au>Ramos, R</au><au>Elmendorf, H</au><au>Kirschner, M W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Xenopus nodal-related gene that acts in synergy with noggin to induce complete secondary axis and notochord formation</atitle><jtitle>Development (Cambridge)</jtitle><addtitle>Development</addtitle><date>1996-10-01</date><risdate>1996</risdate><volume>122</volume><issue>10</issue><spage>3275</spage><epage>3282</epage><pages>3275-3282</pages><issn>0950-1991</issn><eissn>1477-9129</eissn><abstract>Using a paracrine assay to screen for signaling proteins that could respecify ectodermal tissue, we isolated a Xenopus gene related to the mouse gene nodal, a member of the TGFbeta superfamily. The gene is expressed in three regions in the early Xenopus embryo: first in the gastrula organizer, then in two stripes of cells flanking the posterior notochord in late neurulae, and finally in lateral plate mesoderm restricted to the left side of tailbud-stage embryos. Ectopic expression of the gene induces muscle formation in ectodermal explants and partial secondary axes in whole embryos. Together with noggin, another secreted protein also present in the organizer, it induces notochord formation in ectodermal explants and complete secondary axes in whole embryos. These results suggest that the nodal-related gene may act together with noggin to induce axial pattern during gastrulation and also may play a role in left-right asymmetry generation in the post-gastrula embryo.</abstract><cop>England</cop><pub>The Company of Biologists Limited</pub><pmid>8898239</pmid><doi>10.1242/dev.122.10.3275</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0950-1991 |
| ispartof | Development (Cambridge), 1996-10, Vol.122 (10), p.3275-3282 |
| issn | 0950-1991 1477-9129 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Company of Biologists |
| subjects | Animals Axis, Cervical Vertebra - embryology Axis, Cervical Vertebra - physiology Carrier Proteins Cloning, Molecular Ectoderm - physiology Endoderm - physiology Freshwater Gastrula - physiology Gene Expression Nodal Protein Notochord - embryology Notochord - physiology Proteins Transforming Growth Factor beta - genetics Transforming Growth Factor beta - metabolism Xenopus laevis Xenopus laevis - embryology Xenopus laevis - metabolism Xenopus Proteins |
| title | A Xenopus nodal-related gene that acts in synergy with noggin to induce complete secondary axis and notochord formation |
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