A Serum-Free Primary Culture System for Studying Cell-Substrate Interactions during Newt Epidermal Cell Migration

To study the interaction of migrating newt epidermal cells with purified extracellular matrix (ECM) molecules we have developed an in vitro migration assay using pieces of newt skin explanted onto culture dishes coated with various ECM molecules and cultured for 18 h in defined serum-free medium. Ne...

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Veröffentlicht in:	In Vitro Cellular & Developmental Biology 1988-10, Vol.24 (10), p.1023-1030
Hauptverfasser:	Mahan, James T., Donaldson, Donald J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal cells Animal migration behavior Animals Biological and medical sciences Cell Adhesion Cell cultures. Hybridization. Fusion Cell Division Cell Movement Cells, Cultured Collagen - physiology Collagens Culture Media Cultured cells Cycloheximide - pharmacology DNA DNA - biosynthesis Epidermal cells Epidermis Fibrinogen - physiology Fibronectins - physiology Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Glycoproteins - physiology Insulin Insulin - pharmacology Keratins - metabolism Male Molecular and cellular biology Newts Notophthalmus viridescens Protein synthesis Puromycin - pharmacology Salamandridae Skin Skin - cytology Skin Physiological Phenomena Vitronectin
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