DNA Cytometry of Pure Dysgerminomas of the Ovary
SUMMARYThe value of DNA cytometry for predicting the malignant potential of pure dysgerminomas of the ovary was investigated. Feulgen-thionin DNA image cytometry and propidium iodide DNA flow cytometry were performed in isolated nuclei from paraffin-embedded tissue of 25 dysgerminomas. Nineteen were...
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| Veröffentlicht in: | International journal of gynecological pathology 1988-09, Vol.7 (3), p.258-267 |
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| container_title | International journal of gynecological pathology |
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| creator | Oud, Peter S Soeters, Robert P Pahlplatz, Martin M. M Hermkens, Huub G Beck, Hans L. M Reubsaet-Veldhuizen, José Vooijs, G Peter |
| description | SUMMARYThe value of DNA cytometry for predicting the malignant potential of pure dysgerminomas of the ovary was investigated. Feulgen-thionin DNA image cytometry and propidium iodide DNA flow cytometry were performed in isolated nuclei from paraffin-embedded tissue of 25 dysgerminomas. Nineteen were in clinical stage lal, and six were in stage III (two in IIIa and four in IIIb). Eight patients showed recurrences during a 5-year follow-up period and one patient died of the disease. The DNA index (DI; the modal DNA value compared with that of normal cells) of the tumor cells was computed from the image and flow DNA histograms. Comparable DI values, ranging from 1.29 and 3.23, were found with both types of cytometry. No correlation was found with the clinical stage or the recurrence state. Furthermore, it was striking that, although values were found strongly deviating from the normal diploid content (DI = 1.0) suggesting an unfavorable prognosis, the survival rate was relatively high. It can be concluded that prediction of the clinical course of pure dysgerminomas by DNA cytometry does not seem feasible. |
| doi_str_mv | 10.1097/00004347-198809000-00006 |
| format | Article |
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M ; Hermkens, Huub G ; Beck, Hans L. M ; Reubsaet-Veldhuizen, José ; Vooijs, G Peter</creator><creatorcontrib>Oud, Peter S ; Soeters, Robert P ; Pahlplatz, Martin M. M ; Hermkens, Huub G ; Beck, Hans L. M ; Reubsaet-Veldhuizen, José ; Vooijs, G Peter</creatorcontrib><description>SUMMARYThe value of DNA cytometry for predicting the malignant potential of pure dysgerminomas of the ovary was investigated. Feulgen-thionin DNA image cytometry and propidium iodide DNA flow cytometry were performed in isolated nuclei from paraffin-embedded tissue of 25 dysgerminomas. Nineteen were in clinical stage lal, and six were in stage III (two in IIIa and four in IIIb). Eight patients showed recurrences during a 5-year follow-up period and one patient died of the disease. The DNA index (DI; the modal DNA value compared with that of normal cells) of the tumor cells was computed from the image and flow DNA histograms. Comparable DI values, ranging from 1.29 and 3.23, were found with both types of cytometry. No correlation was found with the clinical stage or the recurrence state. Furthermore, it was striking that, although values were found strongly deviating from the normal diploid content (DI = 1.0) suggesting an unfavorable prognosis, the survival rate was relatively high. It can be concluded that prediction of the clinical course of pure dysgerminomas by DNA cytometry does not seem feasible.</description><identifier>ISSN: 0277-1691</identifier><identifier>EISSN: 1538-7151</identifier><identifier>DOI: 10.1097/00004347-198809000-00006</identifier><identifier>PMID: 3182171</identifier><identifier>CODEN: IJGPDR</identifier><language>eng</language><publisher>Philadelphia, PA: International Society of Gynecological Pathologists</publisher><subject>Biological and medical sciences ; Cell Nucleus - analysis ; DNA, Neoplasm - analysis ; Dysgerminoma - analysis ; Dysgerminoma - pathology ; Dysgerminoma - ultrastructure ; Female ; Female genital diseases ; Flow Cytometry ; Gynecology. Andrology. Obstetrics ; Humans ; Medical sciences ; Neoplasm Staging ; Non tumoral diseases ; Ovarian Neoplasms - analysis ; Ovarian Neoplasms - pathology ; Ovarian Neoplasms - ultrastructure</subject><ispartof>International journal of gynecological pathology, 1988-09, Vol.7 (3), p.258-267</ispartof><rights>1988International Society of Gynecological Pathologists</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4506-ce5252e2198f65daa8f6bd82fd103591cd72de24dc2a2f42c1263ac459fe806f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6702739$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3182171$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oud, Peter S</creatorcontrib><creatorcontrib>Soeters, Robert P</creatorcontrib><creatorcontrib>Pahlplatz, Martin M. M</creatorcontrib><creatorcontrib>Hermkens, Huub G</creatorcontrib><creatorcontrib>Beck, Hans L. M</creatorcontrib><creatorcontrib>Reubsaet-Veldhuizen, José</creatorcontrib><creatorcontrib>Vooijs, G Peter</creatorcontrib><title>DNA Cytometry of Pure Dysgerminomas of the Ovary</title><title>International journal of gynecological pathology</title><addtitle>Int J Gynecol Pathol</addtitle><description>SUMMARYThe value of DNA cytometry for predicting the malignant potential of pure dysgerminomas of the ovary was investigated. Feulgen-thionin DNA image cytometry and propidium iodide DNA flow cytometry were performed in isolated nuclei from paraffin-embedded tissue of 25 dysgerminomas. Nineteen were in clinical stage lal, and six were in stage III (two in IIIa and four in IIIb). Eight patients showed recurrences during a 5-year follow-up period and one patient died of the disease. The DNA index (DI; the modal DNA value compared with that of normal cells) of the tumor cells was computed from the image and flow DNA histograms. Comparable DI values, ranging from 1.29 and 3.23, were found with both types of cytometry. No correlation was found with the clinical stage or the recurrence state. Furthermore, it was striking that, although values were found strongly deviating from the normal diploid content (DI = 1.0) suggesting an unfavorable prognosis, the survival rate was relatively high. It can be concluded that prediction of the clinical course of pure dysgerminomas by DNA cytometry does not seem feasible.</description><subject>Biological and medical sciences</subject><subject>Cell Nucleus - analysis</subject><subject>DNA, Neoplasm - analysis</subject><subject>Dysgerminoma - analysis</subject><subject>Dysgerminoma - pathology</subject><subject>Dysgerminoma - ultrastructure</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Flow Cytometry</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Neoplasm Staging</subject><subject>Non tumoral diseases</subject><subject>Ovarian Neoplasms - analysis</subject><subject>Ovarian Neoplasms - pathology</subject><subject>Ovarian Neoplasms - ultrastructure</subject><issn>0277-1691</issn><issn>1538-7151</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1UctOwzAQtBColMInIOWAuAVsJ_HjWLW8pIpygLPlOmsaSJpiJ1T5exwaesOXlWdndkezCEUE3xAs-S0OL01SHhMpBJbhF_cQO0JjkiUi5iQjx2iMKQ8UJskpOvP-A2PCCOMjNEqIoISTMcLz52k065q6gsZ1UW2jl9ZBNO_8O7iq2NSV9j3arCFafmvXnaMTq0sPF0OdoLf7u9fZY7xYPjzNpovYpBlmsYGMZhRo8GdZlmsdyioX1OYEJ5kkJuc0B5rmhmpqU2oIZYkOWmlBYGaTCbrez926-qsF36iq8AbKUm-gbr3iIpVMSBmIYk80rvbegVVbV1TBqSJY9WGpv7DUIaxfiAXp5bCjXVWQH4RDOqF_NfS1N7q0Tm9M4Q80xkO-Se8g3dN2ddmA859luwOn1qDLZq3-O1XyA7lzfzs</recordid><startdate>198809</startdate><enddate>198809</enddate><creator>Oud, Peter S</creator><creator>Soeters, Robert P</creator><creator>Pahlplatz, Martin M. M</creator><creator>Hermkens, Huub G</creator><creator>Beck, Hans L. M</creator><creator>Reubsaet-Veldhuizen, José</creator><creator>Vooijs, G Peter</creator><general>International Society of Gynecological Pathologists</general><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198809</creationdate><title>DNA Cytometry of Pure Dysgerminomas of the Ovary</title><author>Oud, Peter S ; Soeters, Robert P ; Pahlplatz, Martin M. M ; Hermkens, Huub G ; Beck, Hans L. M ; Reubsaet-Veldhuizen, José ; Vooijs, G Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4506-ce5252e2198f65daa8f6bd82fd103591cd72de24dc2a2f42c1263ac459fe806f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Biological and medical sciences</topic><topic>Cell Nucleus - analysis</topic><topic>DNA, Neoplasm - analysis</topic><topic>Dysgerminoma - analysis</topic><topic>Dysgerminoma - pathology</topic><topic>Dysgerminoma - ultrastructure</topic><topic>Female</topic><topic>Female genital diseases</topic><topic>Flow Cytometry</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Neoplasm Staging</topic><topic>Non tumoral diseases</topic><topic>Ovarian Neoplasms - analysis</topic><topic>Ovarian Neoplasms - pathology</topic><topic>Ovarian Neoplasms - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oud, Peter S</creatorcontrib><creatorcontrib>Soeters, Robert P</creatorcontrib><creatorcontrib>Pahlplatz, Martin M. M</creatorcontrib><creatorcontrib>Hermkens, Huub G</creatorcontrib><creatorcontrib>Beck, Hans L. 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M</au><au>Reubsaet-Veldhuizen, José</au><au>Vooijs, G Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA Cytometry of Pure Dysgerminomas of the Ovary</atitle><jtitle>International journal of gynecological pathology</jtitle><addtitle>Int J Gynecol Pathol</addtitle><date>1988-09</date><risdate>1988</risdate><volume>7</volume><issue>3</issue><spage>258</spage><epage>267</epage><pages>258-267</pages><issn>0277-1691</issn><eissn>1538-7151</eissn><coden>IJGPDR</coden><abstract>SUMMARYThe value of DNA cytometry for predicting the malignant potential of pure dysgerminomas of the ovary was investigated. Feulgen-thionin DNA image cytometry and propidium iodide DNA flow cytometry were performed in isolated nuclei from paraffin-embedded tissue of 25 dysgerminomas. Nineteen were in clinical stage lal, and six were in stage III (two in IIIa and four in IIIb). Eight patients showed recurrences during a 5-year follow-up period and one patient died of the disease. The DNA index (DI; the modal DNA value compared with that of normal cells) of the tumor cells was computed from the image and flow DNA histograms. Comparable DI values, ranging from 1.29 and 3.23, were found with both types of cytometry. No correlation was found with the clinical stage or the recurrence state. Furthermore, it was striking that, although values were found strongly deviating from the normal diploid content (DI = 1.0) suggesting an unfavorable prognosis, the survival rate was relatively high. It can be concluded that prediction of the clinical course of pure dysgerminomas by DNA cytometry does not seem feasible.</abstract><cop>Philadelphia, PA</cop><cop>Hagerstown, MD</cop><pub>International Society of Gynecological Pathologists</pub><pmid>3182171</pmid><doi>10.1097/00004347-198809000-00006</doi><tpages>10</tpages></addata></record> |
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| subjects | Biological and medical sciences Cell Nucleus - analysis DNA, Neoplasm - analysis Dysgerminoma - analysis Dysgerminoma - pathology Dysgerminoma - ultrastructure Female Female genital diseases Flow Cytometry Gynecology. Andrology. Obstetrics Humans Medical sciences Neoplasm Staging Non tumoral diseases Ovarian Neoplasms - analysis Ovarian Neoplasms - pathology Ovarian Neoplasms - ultrastructure |
| title | DNA Cytometry of Pure Dysgerminomas of the Ovary |
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