Gene targeting the myf‐5 locus with nlacZ reveals expression of this myogenic factor in mature skeletal muscle fibres as well as early embryonic muscle

We have introduced the nlacZ reporter gene into the locus of the myogenic factor gene myf‐5 by homologous recombination in embryonic stem (ES) cells. Targeted ES clones were injected into precompaction morula, and the β‐galactosidase expression pattern was monitored. These mice permit the sensitive...

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