The human endogenous local anesthetic-like factor (ELLF) is functionally neutralized by serum albumin
The cerebrospinal fluid (CSF) of patients with multiple sclerosis or Guillain-Barré syndrome contains a factor that inhibits excitation of nerve and muscle cells like local anesthetics. CSF samples containing the endogenous local anesthetic-like factor (ELLF) were analyzed by gel filtration chromato...
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| Veröffentlicht in: | Neuroscience letters 1996-09, Vol.216 (1), p.37-40 |
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| container_title | Neuroscience letters |
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| creator | Aulkemeyer, Peter Brinkmeier, Heinrich Wollinsky, Kurt H. Rüdel, Reinhardt |
| description | The cerebrospinal fluid (CSF) of patients with multiple sclerosis or Guillain-Barré syndrome contains a factor that inhibits excitation of nerve and muscle cells like local anesthetics. CSF samples containing the endogenous local anesthetic-like factor (ELLF) were analyzed by gel filtration chromatography and ultraviolet (UV) absorption at 210 nm. The active component was in a single peak corresponding to a molecular weight of 600–800 Da. This peak was decreased and the Na
+ channel blocking activity was neutralized by the addition of 40 g/l human serum albumin to the CSF. When the albumin was separated from the CSF/albumin mixture by acetonitrile treatment, the Na
+ channel blocking activity reappeared. The ELLF and its neutralization may be of relevance for the clinical fluctuations known with these diseases. |
| doi_str_mv | 10.1016/0304-3940(96)12997-9 |
| format | Article |
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+ channel blocking activity was neutralized by the addition of 40 g/l human serum albumin to the CSF. When the albumin was separated from the CSF/albumin mixture by acetonitrile treatment, the Na
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+ channel blocking activity was neutralized by the addition of 40 g/l human serum albumin to the CSF. When the albumin was separated from the CSF/albumin mixture by acetonitrile treatment, the Na
+ channel blocking activity reappeared. The ELLF and its neutralization may be of relevance for the clinical fluctuations known with these diseases.</description><subject>Albumin</subject><subject>Biological and medical sciences</subject><subject>Cerebrospinal Fluid</subject><subject>Chromatography, Gel</subject><subject>Demyelinating Diseases - cerebrospinal fluid</subject><subject>Guillain-Barré syndrome</subject><subject>High performance liquid chromatography</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Multiple sclerosis</subject><subject>Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis</subject><subject>Neurology</subject><subject>NH15-CA2 cells</subject><subject>Polyradiculoneuropathy - cerebrospinal fluid</subject><subject>Protein Binding - physiology</subject><subject>Serum Albumin - physiology</subject><subject>Sodium channel</subject><subject>Sodium Channels - metabolism</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Tumor Cells, Cultured</subject><subject>Whole-cell recording</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2LFDEQhoMo67j6DxRyENk9tCaddCe5LMiyq8KAl_Uc8lFxoun0mnQL46834wxz9FSHeqp43weh15S8p4SOHwgjvGOKkys1XtNeKdGpJ2hDpeg7oUT_FG3OyHP0otYfhJCBDvwCXUipeibHDYKHHeDdOpmMIfv5O-R5rTjNziRsMtRlB0t0XYo_AQfjlrngq7vt9v4ax4rDmt0S52xS2uMM61JMin_AY7vHFco6YZPsOsX8Ej0LJlV4dZqX6Nv93cPt52779dOX24_bzrUwS6eU94T33hvDDlmNtEACHwfBmRTO21G50OowCwDOShb4MDjqlLTGChrYJXp3_PtY5l9rS6-nWB2k1Kq0XlpILqXgfQP5EXRlrrVA0I8lTqbsNSX6YFcf1OmDOq1G_c-uVu3szen_aifw56OTzrZ_e9qb2gyGYrKL9YyxvldE0YbdHDFoLn5HKLq6CNmBjwXcov0c_5_jL9wJlys</recordid><startdate>19960920</startdate><enddate>19960920</enddate><creator>Aulkemeyer, Peter</creator><creator>Brinkmeier, Heinrich</creator><creator>Wollinsky, Kurt H.</creator><creator>Rüdel, Reinhardt</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960920</creationdate><title>The human endogenous local anesthetic-like factor (ELLF) is functionally neutralized by serum albumin</title><author>Aulkemeyer, Peter ; Brinkmeier, Heinrich ; Wollinsky, Kurt H. ; Rüdel, Reinhardt</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-99dd042ddaa30005a8be0f46574387cdb69cf3943beeecb83f455c1c98bab71f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Albumin</topic><topic>Biological and medical sciences</topic><topic>Cerebrospinal Fluid</topic><topic>Chromatography, Gel</topic><topic>Demyelinating Diseases - cerebrospinal fluid</topic><topic>Guillain-Barré syndrome</topic><topic>High performance liquid chromatography</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Multiple sclerosis</topic><topic>Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis</topic><topic>Neurology</topic><topic>NH15-CA2 cells</topic><topic>Polyradiculoneuropathy - cerebrospinal fluid</topic><topic>Protein Binding - physiology</topic><topic>Serum Albumin - physiology</topic><topic>Sodium channel</topic><topic>Sodium Channels - metabolism</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Tumor Cells, Cultured</topic><topic>Whole-cell recording</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aulkemeyer, Peter</creatorcontrib><creatorcontrib>Brinkmeier, Heinrich</creatorcontrib><creatorcontrib>Wollinsky, Kurt H.</creatorcontrib><creatorcontrib>Rüdel, Reinhardt</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aulkemeyer, Peter</au><au>Brinkmeier, Heinrich</au><au>Wollinsky, Kurt H.</au><au>Rüdel, Reinhardt</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The human endogenous local anesthetic-like factor (ELLF) is functionally neutralized by serum albumin</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1996-09-20</date><risdate>1996</risdate><volume>216</volume><issue>1</issue><spage>37</spage><epage>40</epage><pages>37-40</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>The cerebrospinal fluid (CSF) of patients with multiple sclerosis or Guillain-Barré syndrome contains a factor that inhibits excitation of nerve and muscle cells like local anesthetics. CSF samples containing the endogenous local anesthetic-like factor (ELLF) were analyzed by gel filtration chromatography and ultraviolet (UV) absorption at 210 nm. The active component was in a single peak corresponding to a molecular weight of 600–800 Da. This peak was decreased and the Na
+ channel blocking activity was neutralized by the addition of 40 g/l human serum albumin to the CSF. When the albumin was separated from the CSF/albumin mixture by acetonitrile treatment, the Na
+ channel blocking activity reappeared. The ELLF and its neutralization may be of relevance for the clinical fluctuations known with these diseases.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>8892386</pmid><doi>10.1016/0304-3940(96)12997-9</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0304-3940 |
| ispartof | Neuroscience letters, 1996-09, Vol.216 (1), p.37-40 |
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| language | eng |
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| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Albumin Biological and medical sciences Cerebrospinal Fluid Chromatography, Gel Demyelinating Diseases - cerebrospinal fluid Guillain-Barré syndrome High performance liquid chromatography Humans Medical sciences Multiple sclerosis Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis Neurology NH15-CA2 cells Polyradiculoneuropathy - cerebrospinal fluid Protein Binding - physiology Serum Albumin - physiology Sodium channel Sodium Channels - metabolism Spectrophotometry, Ultraviolet Tumor Cells, Cultured Whole-cell recording |
| title | The human endogenous local anesthetic-like factor (ELLF) is functionally neutralized by serum albumin |
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