Elevation of PMN cytosolic free calcium and locomotion stimulated by novel peptides from IL-1-treated human synovial cell cultures
Treatment of human synovial cell cultures with human recombinant interleukin 1 (IL-1) results in the appearance of activity in the supernatant which stimulates human polymorphonuclear neutrophils (PMNs), as assessed by increased chemokinesis and elevation of intracellular free calcium concentration....
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| Veröffentlicht in: | Biochemical and biophysical research communications 1988-09, Vol.155 (3), p.1154-1160 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Watson, M.L. Westwick, J. Fincham, N.J. Camp, R.D.R. |
| description | Treatment of human synovial cell cultures with human recombinant interleukin 1 (IL-1) results in the appearance of activity in the supernatant which stimulates human polymorphonuclear neutrophils (PMNs), as assessed by increased chemokinesis and elevation of intracellular free calcium concentration. IL-1 (or related cytokines) were unable to stimulate these responses. This activity chromatographed as a single peak on C
8 reversed-phase HPLC and subsequent size exclusion HPLC revealed two peaks of chemokinetic activity with apparent molecular masses of ≈ 13kDa and 6kDa. Fractions containing the higher molecular mass material also elevated PMN cytosolic free calcium. The local production of such factors may mediate IL-1-induced PMN accumulation
in vivo. |
| doi_str_mv | 10.1016/S0006-291X(88)81261-0 |
| format | Article |
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8 reversed-phase HPLC and subsequent size exclusion HPLC revealed two peaks of chemokinetic activity with apparent molecular masses of ≈ 13kDa and 6kDa. Fractions containing the higher molecular mass material also elevated PMN cytosolic free calcium. The local production of such factors may mediate IL-1-induced PMN accumulation
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8 reversed-phase HPLC and subsequent size exclusion HPLC revealed two peaks of chemokinetic activity with apparent molecular masses of ≈ 13kDa and 6kDa. Fractions containing the higher molecular mass material also elevated PMN cytosolic free calcium. The local production of such factors may mediate IL-1-induced PMN accumulation
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| ispartof | Biochemical and biophysical research communications, 1988-09, Vol.155 (3), p.1154-1160 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Calcium - metabolism Cell physiology Cells, Cultured Chemotaxis, Leukocyte - drug effects Chromatography, High Pressure Liquid Fundamental and applied biological sciences. Psychology Humans Interleukin-1 - pharmacology Miscellaneous Molecular and cellular biology Neutrophils - metabolism Synovial Fluid - cytology |
| title | Elevation of PMN cytosolic free calcium and locomotion stimulated by novel peptides from IL-1-treated human synovial cell cultures |
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