Expression of pituitary adenylate cyclase activating polypeptide receptors in the early mouse embryo as assessed by reverse transcription polymerase chain reaction and in situ hybridisation
We have examined the expression of mRNA for the pituitary adenylate cyclase activating polypeptide (PACAP) receptor in the mouse from day 9.5 of embryonic development onwards by reverse transcription polymerase chain reaction (RT-PCR) and by in situ hybridisation. PACAP receptor mRNAs were detected...
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| Veröffentlicht in: | Neuroscience letters 1996-09, Vol.216 (1), p.45-48 |
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| creator | Sheward, W.J. Lutz, E.M. Harmar, A.J. |
| description | We have examined the expression of mRNA for the pituitary adenylate cyclase activating polypeptide (PACAP) receptor in the mouse from day 9.5 of embryonic development onwards by reverse transcription polymerase chain reaction (RT-PCR) and by in situ hybridisation. PACAP receptor mRNAs were detected by PCR in the earliest developmental stage examined and thereafter the levels of mRNA for all receptors increased during development. Wholemount in situ hybridisation first showed the expression of the PACAP receptor in the neural tube of 9.5 day old embryos, in later embryos heaviest expression of the PACAP receptor was found in the developing rhombencephalon (day 10.5 and 11.5).These results are consistent with a role for PACAP or a related peptide in the early development of the central nervous system. |
| doi_str_mv | 10.1016/0304-3940(96)13002-0 |
| format | Article |
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PACAP receptor mRNAs were detected by PCR in the earliest developmental stage examined and thereafter the levels of mRNA for all receptors increased during development. Wholemount in situ hybridisation first showed the expression of the PACAP receptor in the neural tube of 9.5 day old embryos, in later embryos heaviest expression of the PACAP receptor was found in the developing rhombencephalon (day 10.5 and 11.5).These results are consistent with a role for PACAP or a related peptide in the early development of the central nervous system.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/0304-3940(96)13002-0</identifier><identifier>PMID: 8892388</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Biological and medical sciences ; Development ; Embryology: invertebrates and vertebrates. Teratology ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental - physiology ; In Situ Hybridization ; Mice ; Mice, Inbred Strains ; Molecular embryology ; Mouse ; Neuropeptides - metabolism ; Neurotransmitter Agents - metabolism ; Oligonucleotide Probes ; Pituitary Adenylate Cyclase-Activating Polypeptide ; Pituitary Gland - embryology ; Pituitary Gland - metabolism ; Polymerase Chain Reaction ; Pregnancy ; Rats ; Receptors ; Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide ; Receptors, Pituitary Hormone - biosynthesis ; Receptors, Pituitary Hormone - genetics ; Rhombencephalon - embryology ; Rhombencephalon - metabolism ; RNA, Messenger - biosynthesis ; Vasoactive intestinal peptide</subject><ispartof>Neuroscience letters, 1996-09, Vol.216 (1), p.45-48</ispartof><rights>1996</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-cc3493c74408c449052f35e703f73d3fce38aed5ef3bd45d51ce2ced1c62cfbe3</citedby><cites>FETCH-LOGICAL-c386t-cc3493c74408c449052f35e703f73d3fce38aed5ef3bd45d51ce2ced1c62cfbe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0304-3940(96)13002-0$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3229411$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8892388$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sheward, W.J.</creatorcontrib><creatorcontrib>Lutz, E.M.</creatorcontrib><creatorcontrib>Harmar, A.J.</creatorcontrib><title>Expression of pituitary adenylate cyclase activating polypeptide receptors in the early mouse embryo as assessed by reverse transcription polymerase chain reaction and in situ hybridisation</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>We have examined the expression of mRNA for the pituitary adenylate cyclase activating polypeptide (PACAP) receptor in the mouse from day 9.5 of embryonic development onwards by reverse transcription polymerase chain reaction (RT-PCR) and by in situ hybridisation. PACAP receptor mRNAs were detected by PCR in the earliest developmental stage examined and thereafter the levels of mRNA for all receptors increased during development. Wholemount in situ hybridisation first showed the expression of the PACAP receptor in the neural tube of 9.5 day old embryos, in later embryos heaviest expression of the PACAP receptor was found in the developing rhombencephalon (day 10.5 and 11.5).These results are consistent with a role for PACAP or a related peptide in the early development of the central nervous system.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Development</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental - physiology</subject><subject>In Situ Hybridization</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Molecular embryology</subject><subject>Mouse</subject><subject>Neuropeptides - metabolism</subject><subject>Neurotransmitter Agents - metabolism</subject><subject>Oligonucleotide Probes</subject><subject>Pituitary Adenylate Cyclase-Activating Polypeptide</subject><subject>Pituitary Gland - embryology</subject><subject>Pituitary Gland - metabolism</subject><subject>Polymerase Chain Reaction</subject><subject>Pregnancy</subject><subject>Rats</subject><subject>Receptors</subject><subject>Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide</subject><subject>Receptors, Pituitary Hormone - biosynthesis</subject><subject>Receptors, Pituitary Hormone - genetics</subject><subject>Rhombencephalon - embryology</subject><subject>Rhombencephalon - metabolism</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Vasoactive intestinal peptide</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UcuqFDEQDaJcx9E_UMhCRBetSSfdnd4Icrk-4IIbXYd0pdqJ9MskM9gf579Z7QyzFAIJOafOqarD2HMp3koh63dCCV2oVovXbf1GKiHKQjxgO2masmjapnzIdlfKY_YkpZ9CiEpW-obdGNOWypgd-3P3e4mYUpgnPvd8CfkYsosrdx6ndXAZOawwuITcQQ4nl8P0gy_zsC645OCRRwR6zTHxMPF8QI4uDisf5yPV4NjFdeYu0Ulkg553K5WcMBKao5sSxEBCZL-Jjhg3Kzg4Eou4WRLiJr-JJ2qOH9YuBh-S25Cn7FHvhoTPLveeff949-32c3H_9dOX2w_3BShT5wJA6VZBo7UwoHUrqrJXFTZC9Y3yqgdUxqGvsFed15WvJGAJ6CXUJfQdqj17ddZd4vzriCnbMSTAYXAT0py2MdpUtdZE1GcixDmliL1dYhhpn1YKu6Vmt0jsFolta_svNfrasxcX_WM3or8WXWIi_OUFdwnc0NPeIKQrTZVlq6Uk2vszDWkXp4DRJgg40SSBUsrWz-H_ffwF6au6eg</recordid><startdate>19960920</startdate><enddate>19960920</enddate><creator>Sheward, W.J.</creator><creator>Lutz, E.M.</creator><creator>Harmar, A.J.</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19960920</creationdate><title>Expression of pituitary adenylate cyclase activating polypeptide receptors in the early mouse embryo as assessed by reverse transcription polymerase chain reaction and in situ hybridisation</title><author>Sheward, W.J. ; Lutz, E.M. ; Harmar, A.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-cc3493c74408c449052f35e703f73d3fce38aed5ef3bd45d51ce2ced1c62cfbe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Development</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental - physiology</topic><topic>In Situ Hybridization</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Molecular embryology</topic><topic>Mouse</topic><topic>Neuropeptides - metabolism</topic><topic>Neurotransmitter Agents - metabolism</topic><topic>Oligonucleotide Probes</topic><topic>Pituitary Adenylate Cyclase-Activating Polypeptide</topic><topic>Pituitary Gland - embryology</topic><topic>Pituitary Gland - metabolism</topic><topic>Polymerase Chain Reaction</topic><topic>Pregnancy</topic><topic>Rats</topic><topic>Receptors</topic><topic>Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide</topic><topic>Receptors, Pituitary Hormone - biosynthesis</topic><topic>Receptors, Pituitary Hormone - genetics</topic><topic>Rhombencephalon - embryology</topic><topic>Rhombencephalon - metabolism</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Vasoactive intestinal peptide</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sheward, W.J.</creatorcontrib><creatorcontrib>Lutz, E.M.</creatorcontrib><creatorcontrib>Harmar, A.J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sheward, W.J.</au><au>Lutz, E.M.</au><au>Harmar, A.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of pituitary adenylate cyclase activating polypeptide receptors in the early mouse embryo as assessed by reverse transcription polymerase chain reaction and in situ hybridisation</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>1996-09-20</date><risdate>1996</risdate><volume>216</volume><issue>1</issue><spage>45</spage><epage>48</epage><pages>45-48</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>We have examined the expression of mRNA for the pituitary adenylate cyclase activating polypeptide (PACAP) receptor in the mouse from day 9.5 of embryonic development onwards by reverse transcription polymerase chain reaction (RT-PCR) and by in situ hybridisation. PACAP receptor mRNAs were detected by PCR in the earliest developmental stage examined and thereafter the levels of mRNA for all receptors increased during development. Wholemount in situ hybridisation first showed the expression of the PACAP receptor in the neural tube of 9.5 day old embryos, in later embryos heaviest expression of the PACAP receptor was found in the developing rhombencephalon (day 10.5 and 11.5).These results are consistent with a role for PACAP or a related peptide in the early development of the central nervous system.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>8892388</pmid><doi>10.1016/0304-3940(96)13002-0</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Neuroscience letters, 1996-09, Vol.216 (1), p.45-48 |
| issn | 0304-3940 1872-7972 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Biological and medical sciences Development Embryology: invertebrates and vertebrates. Teratology Female Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental - physiology In Situ Hybridization Mice Mice, Inbred Strains Molecular embryology Mouse Neuropeptides - metabolism Neurotransmitter Agents - metabolism Oligonucleotide Probes Pituitary Adenylate Cyclase-Activating Polypeptide Pituitary Gland - embryology Pituitary Gland - metabolism Polymerase Chain Reaction Pregnancy Rats Receptors Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide Receptors, Pituitary Hormone - biosynthesis Receptors, Pituitary Hormone - genetics Rhombencephalon - embryology Rhombencephalon - metabolism RNA, Messenger - biosynthesis Vasoactive intestinal peptide |
| title | Expression of pituitary adenylate cyclase activating polypeptide receptors in the early mouse embryo as assessed by reverse transcription polymerase chain reaction and in situ hybridisation |
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