Human immunodeficiency virus type 1 neutralizing antibody serotyping using serum pools and an infectivity reduction assay
Classification of human immunodeficiency virus type 1 (HIV-1) by neutralization serotype may be important for the design of active and passive immunization strategies. Neutralizing antibody serotyping is hindered by the lack of standard reagents and assay format, and by the weak activity of many ind...
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| Veröffentlicht in: | AIDS research and human retroviruses 1996-09, Vol.12 (14), p.1319-1328 |
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| creator | MASCOLA, J. R LOUDER, M. K MCCUTCHAN, F. E BIRX, D. L BURKE, D. S SURMAN, S. R VANCOTT, T. C XIAO FANG YU BRADAC, J PORTER, K. R NELSON, K. E GIRARD, M MCNEIL, J. G |
| description | Classification of human immunodeficiency virus type 1 (HIV-1) by neutralization serotype may be important for the design of active and passive immunization strategies. Neutralizing antibody serotyping is hindered by the lack of standard reagents and assay format, and by the weak activity of many individual sera. To facilitate cross-clade neutralization analysis, we used an infectivity reduction assay (IRA) and selected clade-specific serum (or plasma) pools from subjects infected with clade B and E HIV-1, respectively. Several serum pools were utilized; some were selected for strong neutralizing activity against intraclade viruses and others were derived from conveniently available samples. Against a panel of 51 clade B and E viruses, serum pools displayed strong neutralization of most intraclade viruses and significantly diminished cross-clade neutralization. Results were confirmed against a blinded panel of 20 viruses. The data indicate that the phylogenetic classification of virus subtypes B and E corresponds to two distinct neutralization serotypes. This approach to neutralizing antibody serotyping may be useful in defining the antigenic relationship among viruses from other clades. |
| doi_str_mv | 10.1089/aid.1996.12.1319 |
| format | Article |
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R ; LOUDER, M. K ; MCCUTCHAN, F. E ; BIRX, D. L ; BURKE, D. S ; SURMAN, S. R ; VANCOTT, T. C ; XIAO FANG YU ; BRADAC, J ; PORTER, K. R ; NELSON, K. E ; GIRARD, M ; MCNEIL, J. G</creator><creatorcontrib>MASCOLA, J. R ; LOUDER, M. K ; MCCUTCHAN, F. E ; BIRX, D. L ; BURKE, D. S ; SURMAN, S. R ; VANCOTT, T. C ; XIAO FANG YU ; BRADAC, J ; PORTER, K. R ; NELSON, K. E ; GIRARD, M ; MCNEIL, J. G</creatorcontrib><description>Classification of human immunodeficiency virus type 1 (HIV-1) by neutralization serotype may be important for the design of active and passive immunization strategies. Neutralizing antibody serotyping is hindered by the lack of standard reagents and assay format, and by the weak activity of many individual sera. To facilitate cross-clade neutralization analysis, we used an infectivity reduction assay (IRA) and selected clade-specific serum (or plasma) pools from subjects infected with clade B and E HIV-1, respectively. Several serum pools were utilized; some were selected for strong neutralizing activity against intraclade viruses and others were derived from conveniently available samples. Against a panel of 51 clade B and E viruses, serum pools displayed strong neutralization of most intraclade viruses and significantly diminished cross-clade neutralization. Results were confirmed against a blinded panel of 20 viruses. The data indicate that the phylogenetic classification of virus subtypes B and E corresponds to two distinct neutralization serotypes. 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Psychology ; HIV Envelope Protein gp120 - metabolism ; HIV Envelope Protein gp160 - metabolism ; HIV Seropositivity - diagnosis ; human immunodeficiency virus ; Humans ; Microbiology ; Morphology, structure, chemical composition, physicochemical properties ; Neutralization Tests ; Peptide Fragments - metabolism ; Serotyping - methods ; Virology</subject><ispartof>AIDS research and human retroviruses, 1996-09, Vol.12 (14), p.1319-1328</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c354t-6646bb0828ecf7f57af89813269384e41615bb92fe500ba3190d537d521e6cc43</citedby><cites>FETCH-LOGICAL-c354t-6646bb0828ecf7f57af89813269384e41615bb92fe500ba3190d537d521e6cc43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3029,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3232033$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8891111$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MASCOLA, J. R</creatorcontrib><creatorcontrib>LOUDER, M. K</creatorcontrib><creatorcontrib>MCCUTCHAN, F. E</creatorcontrib><creatorcontrib>BIRX, D. L</creatorcontrib><creatorcontrib>BURKE, D. S</creatorcontrib><creatorcontrib>SURMAN, S. R</creatorcontrib><creatorcontrib>VANCOTT, T. C</creatorcontrib><creatorcontrib>XIAO FANG YU</creatorcontrib><creatorcontrib>BRADAC, J</creatorcontrib><creatorcontrib>PORTER, K. R</creatorcontrib><creatorcontrib>NELSON, K. E</creatorcontrib><creatorcontrib>GIRARD, M</creatorcontrib><creatorcontrib>MCNEIL, J. G</creatorcontrib><title>Human immunodeficiency virus type 1 neutralizing antibody serotyping using serum pools and an infectivity reduction assay</title><title>AIDS research and human retroviruses</title><addtitle>AIDS Res Hum Retroviruses</addtitle><description>Classification of human immunodeficiency virus type 1 (HIV-1) by neutralization serotype may be important for the design of active and passive immunization strategies. Neutralizing antibody serotyping is hindered by the lack of standard reagents and assay format, and by the weak activity of many individual sera. To facilitate cross-clade neutralization analysis, we used an infectivity reduction assay (IRA) and selected clade-specific serum (or plasma) pools from subjects infected with clade B and E HIV-1, respectively. Several serum pools were utilized; some were selected for strong neutralizing activity against intraclade viruses and others were derived from conveniently available samples. Against a panel of 51 clade B and E viruses, serum pools displayed strong neutralization of most intraclade viruses and significantly diminished cross-clade neutralization. Results were confirmed against a blinded panel of 20 viruses. The data indicate that the phylogenetic classification of virus subtypes B and E corresponds to two distinct neutralization serotypes. 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Psychology</subject><subject>HIV Envelope Protein gp120 - metabolism</subject><subject>HIV Envelope Protein gp160 - metabolism</subject><subject>HIV Seropositivity - diagnosis</subject><subject>human immunodeficiency virus</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Morphology, structure, chemical composition, physicochemical properties</subject><subject>Neutralization Tests</subject><subject>Peptide Fragments - metabolism</subject><subject>Serotyping - methods</subject><subject>Virology</subject><issn>0889-2229</issn><issn>1931-8405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb1rHDEQxUVIcC52-jQBFSHdXjT62JXKYJLYYHCT1EKrlYzMrnSRVobNX28tPtxGxWh485tXzEPoE5AjEKm-mTAdQan-CPQIDNQbdADFoJOciLfoQKRUHaVUvUcfSnkkhChKxQW6aDq0d0DbTV1MxGFZakyT88EGF-2Gn0KuBa_byWHA0dU1mzn8C_EBm7iGMU0bLi6nBuxaLXttQl3wKaW5NGrCu2_0zq7hKawbzm6qrU8Rm1LMdoXeeTMX9_H8X6I_P3_8vr7p7u5_3V5_v-ssE3zt-p7340gklc76wYvBeKkkMNorJrnj0IMYR0W9E4SMpt2ATIINk6Dgems5u0RfX3xPOf2trqx6CcW6eTbRpVr0ILnkgqv_giD6AahgDSQvoM2plOy8PuWwmLxpIHqPRbdY9B6LBqr3WNrK57N3HRc3vS6cc2jzL-e5KdbMPptoQ3nFGGWUMMaeAYGSl6o</recordid><startdate>19960920</startdate><enddate>19960920</enddate><creator>MASCOLA, J. 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Psychology</topic><topic>HIV Envelope Protein gp120 - metabolism</topic><topic>HIV Envelope Protein gp160 - metabolism</topic><topic>HIV Seropositivity - diagnosis</topic><topic>human immunodeficiency virus</topic><topic>Humans</topic><topic>Microbiology</topic><topic>Morphology, structure, chemical composition, physicochemical properties</topic><topic>Neutralization Tests</topic><topic>Peptide Fragments - metabolism</topic><topic>Serotyping - methods</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MASCOLA, J. R</creatorcontrib><creatorcontrib>LOUDER, M. K</creatorcontrib><creatorcontrib>MCCUTCHAN, F. E</creatorcontrib><creatorcontrib>BIRX, D. L</creatorcontrib><creatorcontrib>BURKE, D. S</creatorcontrib><creatorcontrib>SURMAN, S. R</creatorcontrib><creatorcontrib>VANCOTT, T. C</creatorcontrib><creatorcontrib>XIAO FANG YU</creatorcontrib><creatorcontrib>BRADAC, J</creatorcontrib><creatorcontrib>PORTER, K. R</creatorcontrib><creatorcontrib>NELSON, K. E</creatorcontrib><creatorcontrib>GIRARD, M</creatorcontrib><creatorcontrib>MCNEIL, J. G</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>AIDS research and human retroviruses</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MASCOLA, J. R</au><au>LOUDER, M. K</au><au>MCCUTCHAN, F. E</au><au>BIRX, D. L</au><au>BURKE, D. S</au><au>SURMAN, S. R</au><au>VANCOTT, T. C</au><au>XIAO FANG YU</au><au>BRADAC, J</au><au>PORTER, K. R</au><au>NELSON, K. E</au><au>GIRARD, M</au><au>MCNEIL, J. G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human immunodeficiency virus type 1 neutralizing antibody serotyping using serum pools and an infectivity reduction assay</atitle><jtitle>AIDS research and human retroviruses</jtitle><addtitle>AIDS Res Hum Retroviruses</addtitle><date>1996-09-20</date><risdate>1996</risdate><volume>12</volume><issue>14</issue><spage>1319</spage><epage>1328</epage><pages>1319-1328</pages><issn>0889-2229</issn><eissn>1931-8405</eissn><coden>ARHRE7</coden><abstract>Classification of human immunodeficiency virus type 1 (HIV-1) by neutralization serotype may be important for the design of active and passive immunization strategies. Neutralizing antibody serotyping is hindered by the lack of standard reagents and assay format, and by the weak activity of many individual sera. To facilitate cross-clade neutralization analysis, we used an infectivity reduction assay (IRA) and selected clade-specific serum (or plasma) pools from subjects infected with clade B and E HIV-1, respectively. Several serum pools were utilized; some were selected for strong neutralizing activity against intraclade viruses and others were derived from conveniently available samples. Against a panel of 51 clade B and E viruses, serum pools displayed strong neutralization of most intraclade viruses and significantly diminished cross-clade neutralization. Results were confirmed against a blinded panel of 20 viruses. The data indicate that the phylogenetic classification of virus subtypes B and E corresponds to two distinct neutralization serotypes. This approach to neutralizing antibody serotyping may be useful in defining the antigenic relationship among viruses from other clades.</abstract><cop>Larchmont, NY</cop><pub>Liebert</pub><pmid>8891111</pmid><doi>10.1089/aid.1996.12.1319</doi><tpages>10</tpages></addata></record> |
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| ispartof | AIDS research and human retroviruses, 1996-09, Vol.12 (14), p.1319-1328 |
| issn | 0889-2229 1931-8405 |
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| source | Mary Ann Liebert Online Subscription; MEDLINE |
| subjects | AIDS/HIV Biological and medical sciences Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology HIV Envelope Protein gp120 - metabolism HIV Envelope Protein gp160 - metabolism HIV Seropositivity - diagnosis human immunodeficiency virus Humans Microbiology Morphology, structure, chemical composition, physicochemical properties Neutralization Tests Peptide Fragments - metabolism Serotyping - methods Virology |
| title | Human immunodeficiency virus type 1 neutralizing antibody serotyping using serum pools and an infectivity reduction assay |
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