Scrambled ribosomal RNA gene pieces in chlamydomonas reinhardtii mitochondrial DNA
We describe here a bizarre organization of the single large subunit (LSU) and small subunit (SSU) rRNA genes in the mitochondrial DNA of Chlamydomonas reinhardtii. Each gene is discontinuous, with gene pieces encoding specific rRNA domains (“modules”) interspersed with one another and with intact pr...
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| Veröffentlicht in: | Cell 1988-11, Vol.55 (3), p.399-411 |
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| creator | Boer, Poppo H. Gray, Michael W. |
| description | We describe here a bizarre organization of the single large subunit (LSU) and small subunit (SSU) rRNA genes in the mitochondrial DNA of Chlamydomonas reinhardtii. Each gene is discontinuous, with gene pieces encoding specific rRNA domains (“modules”) interspersed with one another and with intact protein coding and tRNA genes throughout a 6 kbp region. Transcript mapping experiments reveal the presence of abundant small rRNAs whose sizes approximate the sizes of the modules encoding them. Evidently, rRNA splicing does not occur in this system; instead, secondary structure modeling supports the view that the SSU and LSU rRNAs each functions as a noncovalent network of small RNAs, rather than as a single covalently continuous molecule. We propose that such a modular pattern may reflect the structure of the primordial ribosome. |
| doi_str_mv | 10.1016/0092-8674(88)90026-8 |
| format | Article |
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Each gene is discontinuous, with gene pieces encoding specific rRNA domains (“modules”) interspersed with one another and with intact protein coding and tRNA genes throughout a 6 kbp region. Transcript mapping experiments reveal the presence of abundant small rRNAs whose sizes approximate the sizes of the modules encoding them. Evidently, rRNA splicing does not occur in this system; instead, secondary structure modeling supports the view that the SSU and LSU rRNAs each functions as a noncovalent network of small RNAs, rather than as a single covalently continuous molecule. We propose that such a modular pattern may reflect the structure of the primordial ribosome.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/0092-8674(88)90026-8</identifier><identifier>PMID: 2846180</identifier><identifier>CODEN: CELLB5</identifier><language>eng</language><publisher>Cambridge, MA: Elsevier Inc</publisher><subject>Biological and medical sciences ; Blotting, Northern ; Chlamydomonas - genetics ; Chlamydomonas reinhardtii ; Chromosome Mapping ; DNA Restriction Enzymes ; DNA, Mitochondrial - genetics ; DNA, Ribosomal - genetics ; Endonucleases ; Fundamental and applied biological sciences. Psychology ; Genes ; Genetic code ; Molecular and cellular biology ; Molecular genetics ; Nucleic Acid Conformation ; RNA, Ribosomal - genetics ; RNA, Ribosomal, 16S - genetics ; RNA, Ribosomal, 23S - genetics ; RNA, Transfer - genetics ; Single-Strand Specific DNA and RNA Endonucleases ; Transcription, Genetic</subject><ispartof>Cell, 1988-11, Vol.55 (3), p.399-411</ispartof><rights>1988</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-2da655f2e44248d12d25cd9cedad7d3efbb879632e119916b33789f55562090a3</citedby><cites>FETCH-LOGICAL-c417t-2da655f2e44248d12d25cd9cedad7d3efbb879632e119916b33789f55562090a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0092-8674(88)90026-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6811900$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2846180$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boer, Poppo H.</creatorcontrib><creatorcontrib>Gray, Michael W.</creatorcontrib><title>Scrambled ribosomal RNA gene pieces in chlamydomonas reinhardtii mitochondrial DNA</title><title>Cell</title><addtitle>Cell</addtitle><description>We describe here a bizarre organization of the single large subunit (LSU) and small subunit (SSU) rRNA genes in the mitochondrial DNA of Chlamydomonas reinhardtii. Each gene is discontinuous, with gene pieces encoding specific rRNA domains (“modules”) interspersed with one another and with intact protein coding and tRNA genes throughout a 6 kbp region. Transcript mapping experiments reveal the presence of abundant small rRNAs whose sizes approximate the sizes of the modules encoding them. Evidently, rRNA splicing does not occur in this system; instead, secondary structure modeling supports the view that the SSU and LSU rRNAs each functions as a noncovalent network of small RNAs, rather than as a single covalently continuous molecule. We propose that such a modular pattern may reflect the structure of the primordial ribosome.</description><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Chlamydomonas - genetics</subject><subject>Chlamydomonas reinhardtii</subject><subject>Chromosome Mapping</subject><subject>DNA Restriction Enzymes</subject><subject>DNA, Mitochondrial - genetics</subject><subject>DNA, Ribosomal - genetics</subject><subject>Endonucleases</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genetic code</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Nucleic Acid Conformation</subject><subject>RNA, Ribosomal - genetics</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>RNA, Ribosomal, 23S - genetics</subject><subject>RNA, Transfer - genetics</subject><subject>Single-Strand Specific DNA and RNA Endonucleases</subject><subject>Transcription, Genetic</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFO3DAQhi1EBVvKG4CUA6rKIWA7jjO-VFrRApXQIm3L2XLsCWuUxFs7W4m3J-mu9ginOcz3j_75CDlj9IpRJq8pVTwHWYlvAJeKUi5zOCAzRlWVC1bxQzLbI8fkc0ovlFIoy_KIHHEQkgGdkeVvG01Xt-iy6OuQQmfabLmYZ8_YY7b2aDFlvs_sqjXdqwtd6E3KIvp-ZaIbvM86PwS7Cr2Lfoz-WMy_kE-NaROe7uYJebr9-efmPn94vPt1M3_I7dhuyLkzsiwbjkJwAY5xx0vrlEVnXOUKbOoaKiULjowpxWRdFBWoZuwvOVXUFCfk6_buOoa_G0yD7nyy2Lamx7BJugIBRSnYhyArqeQgYQTFFrQxpBSx0evoOxNfNaN6cq4noXoSqgH0f-d6ip3v7m_qDt0-tJM87i92e5OsaZtoeuvTHpMwPkgn7PsWw1HaP49RJ-uxH4X4iHbQLvj3e7wBvBWcTw</recordid><startdate>19881104</startdate><enddate>19881104</enddate><creator>Boer, Poppo H.</creator><creator>Gray, Michael W.</creator><general>Elsevier Inc</general><general>Cell Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19881104</creationdate><title>Scrambled ribosomal RNA gene pieces in chlamydomonas reinhardtii mitochondrial DNA</title><author>Boer, Poppo H. ; Gray, Michael W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-2da655f2e44248d12d25cd9cedad7d3efbb879632e119916b33789f55562090a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Chlamydomonas - genetics</topic><topic>Chlamydomonas reinhardtii</topic><topic>Chromosome Mapping</topic><topic>DNA Restriction Enzymes</topic><topic>DNA, Mitochondrial - genetics</topic><topic>DNA, Ribosomal - genetics</topic><topic>Endonucleases</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genetic code</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Nucleic Acid Conformation</topic><topic>RNA, Ribosomal - genetics</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>RNA, Ribosomal, 23S - genetics</topic><topic>RNA, Transfer - genetics</topic><topic>Single-Strand Specific DNA and RNA Endonucleases</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boer, Poppo H.</creatorcontrib><creatorcontrib>Gray, Michael W.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boer, Poppo H.</au><au>Gray, Michael W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Scrambled ribosomal RNA gene pieces in chlamydomonas reinhardtii mitochondrial DNA</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>1988-11-04</date><risdate>1988</risdate><volume>55</volume><issue>3</issue><spage>399</spage><epage>411</epage><pages>399-411</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><coden>CELLB5</coden><abstract>We describe here a bizarre organization of the single large subunit (LSU) and small subunit (SSU) rRNA genes in the mitochondrial DNA of Chlamydomonas reinhardtii. Each gene is discontinuous, with gene pieces encoding specific rRNA domains (“modules”) interspersed with one another and with intact protein coding and tRNA genes throughout a 6 kbp region. Transcript mapping experiments reveal the presence of abundant small rRNAs whose sizes approximate the sizes of the modules encoding them. Evidently, rRNA splicing does not occur in this system; instead, secondary structure modeling supports the view that the SSU and LSU rRNAs each functions as a noncovalent network of small RNAs, rather than as a single covalently continuous molecule. We propose that such a modular pattern may reflect the structure of the primordial ribosome.</abstract><cop>Cambridge, MA</cop><pub>Elsevier Inc</pub><pmid>2846180</pmid><doi>10.1016/0092-8674(88)90026-8</doi><tpages>13</tpages></addata></record> |
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| ispartof | Cell, 1988-11, Vol.55 (3), p.399-411 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Biological and medical sciences Blotting, Northern Chlamydomonas - genetics Chlamydomonas reinhardtii Chromosome Mapping DNA Restriction Enzymes DNA, Mitochondrial - genetics DNA, Ribosomal - genetics Endonucleases Fundamental and applied biological sciences. Psychology Genes Genetic code Molecular and cellular biology Molecular genetics Nucleic Acid Conformation RNA, Ribosomal - genetics RNA, Ribosomal, 16S - genetics RNA, Ribosomal, 23S - genetics RNA, Transfer - genetics Single-Strand Specific DNA and RNA Endonucleases Transcription, Genetic |
| title | Scrambled ribosomal RNA gene pieces in chlamydomonas reinhardtii mitochondrial DNA |
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