Expression of c-KIT and its ligand, stem cell factor, in normal and subfertile human testicular tissue
The c‐KIT proto‐oncogene encodes for a transmembrane receptor and is associated with maturation of several cell types, including germ cells. The ligand of the receptor has been identified as stem cell factor (SCF). Loss or alteration of the expression of either of these factors leads to anemia, albi...
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Veröffentlicht in: | Journal of andrology 1996-07, Vol.17 (4), p.403-408 |
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description | The c‐KIT proto‐oncogene encodes for a transmembrane receptor and is associated with maturation of several cell types, including germ cells. The ligand of the receptor has been identified as stem cell factor (SCF). Loss or alteration of the expression of either of these factors leads to anemia, albinism, and/or sterility in mice. We examined the expression of c‐KIT and SCF by immunohistochemistry in specimens from normal and infertile human testis. All specimens were obtained in the evaluation of male subfertility. We were able to demonstrate staining for c‐KIT in Leydig cells in all specimens. Normal testis stained for c‐KIT in the cytoplasm of early spermatogenic cells, as well as the acrosomal granules of the round spermatids and the acrosome of testicular spermatozoa. However, staining in testis demonstrating maturation arrest failed to demonstrate acrosomal staining, and Sertoli‐only specimens demonstrated staining for c‐KIT in Leydig cells only. The results for SCF demonstrated an overall uniform staining of Leydig cells in all specimens. The intensity of staining of Sertoli cells increased from normal to maturation arrest to Sertoli‐only specimens. Germ cell staining was consistently negative. We hypothesize that these staining patterns for SCF are due to either lack of staining of the receptor‐ligand complex or overexpression of the kit ligand in tissue that does not express the kit receptor. It appears that the c‐kit receptor is expressed in the acrosome of developing germ cells, as well as in Leydig cells and early spermatogenic cells, suggesting a role in the acrosome reaction, as well as germ cell maturation and differentiation. |
doi_str_mv | 10.1002/j.1939-4640.1996.tb01806.x |
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I ; Feng, H. L ; Cohen, M. B ; Sandra, A</creator><creatorcontrib>Sandlow, J. I ; Feng, H. L ; Cohen, M. B ; Sandra, A</creatorcontrib><description>The c‐KIT proto‐oncogene encodes for a transmembrane receptor and is associated with maturation of several cell types, including germ cells. The ligand of the receptor has been identified as stem cell factor (SCF). Loss or alteration of the expression of either of these factors leads to anemia, albinism, and/or sterility in mice. We examined the expression of c‐KIT and SCF by immunohistochemistry in specimens from normal and infertile human testis. All specimens were obtained in the evaluation of male subfertility. We were able to demonstrate staining for c‐KIT in Leydig cells in all specimens. Normal testis stained for c‐KIT in the cytoplasm of early spermatogenic cells, as well as the acrosomal granules of the round spermatids and the acrosome of testicular spermatozoa. However, staining in testis demonstrating maturation arrest failed to demonstrate acrosomal staining, and Sertoli‐only specimens demonstrated staining for c‐KIT in Leydig cells only. The results for SCF demonstrated an overall uniform staining of Leydig cells in all specimens. The intensity of staining of Sertoli cells increased from normal to maturation arrest to Sertoli‐only specimens. Germ cell staining was consistently negative. We hypothesize that these staining patterns for SCF are due to either lack of staining of the receptor‐ligand complex or overexpression of the kit ligand in tissue that does not express the kit receptor. It appears that the c‐kit receptor is expressed in the acrosome of developing germ cells, as well as in Leydig cells and early spermatogenic cells, suggesting a role in the acrosome reaction, as well as germ cell maturation and differentiation.</description><identifier>ISSN: 0196-3635</identifier><identifier>EISSN: 1939-4640</identifier><identifier>DOI: 10.1002/j.1939-4640.1996.tb01806.x</identifier><identifier>PMID: 8889703</identifier><identifier>CODEN: JOAND3</identifier><language>eng</language><publisher>Oxford, UK: Am Soc Andrology</publisher><subject>Adult ; Biological and medical sciences ; Birth control ; Germ cells ; Gynecology. Andrology. Obstetrics ; Humans ; Immunohistochemistry ; Male ; Medical sciences ; Proto-Oncogene Proteins c-kit - biosynthesis ; proto‐oncogenes ; Spermatogonia - chemistry ; Staining and Labeling ; Stem Cell Factor - biosynthesis ; stem cells ; Sterility. Assisted procreation ; testis ; Testis - chemistry ; Testis - metabolism</subject><ispartof>Journal of andrology, 1996-07, Vol.17 (4), p.403-408</ispartof><rights>1996 American Society of Andrology</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5486-4f3e1f978e1eadc90782ef830892ec6ca7b8125da3e6ca3f86006edc536c62803</citedby><cites>FETCH-LOGICAL-c5486-4f3e1f978e1eadc90782ef830892ec6ca7b8125da3e6ca3f86006edc536c62803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3242104$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8889703$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sandlow, J. I</creatorcontrib><creatorcontrib>Feng, H. L</creatorcontrib><creatorcontrib>Cohen, M. B</creatorcontrib><creatorcontrib>Sandra, A</creatorcontrib><title>Expression of c-KIT and its ligand, stem cell factor, in normal and subfertile human testicular tissue</title><title>Journal of andrology</title><addtitle>J Androl</addtitle><description>The c‐KIT proto‐oncogene encodes for a transmembrane receptor and is associated with maturation of several cell types, including germ cells. The ligand of the receptor has been identified as stem cell factor (SCF). Loss or alteration of the expression of either of these factors leads to anemia, albinism, and/or sterility in mice. We examined the expression of c‐KIT and SCF by immunohistochemistry in specimens from normal and infertile human testis. All specimens were obtained in the evaluation of male subfertility. We were able to demonstrate staining for c‐KIT in Leydig cells in all specimens. Normal testis stained for c‐KIT in the cytoplasm of early spermatogenic cells, as well as the acrosomal granules of the round spermatids and the acrosome of testicular spermatozoa. However, staining in testis demonstrating maturation arrest failed to demonstrate acrosomal staining, and Sertoli‐only specimens demonstrated staining for c‐KIT in Leydig cells only. The results for SCF demonstrated an overall uniform staining of Leydig cells in all specimens. The intensity of staining of Sertoli cells increased from normal to maturation arrest to Sertoli‐only specimens. Germ cell staining was consistently negative. We hypothesize that these staining patterns for SCF are due to either lack of staining of the receptor‐ligand complex or overexpression of the kit ligand in tissue that does not express the kit receptor. It appears that the c‐kit receptor is expressed in the acrosome of developing germ cells, as well as in Leydig cells and early spermatogenic cells, suggesting a role in the acrosome reaction, as well as germ cell maturation and differentiation.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Birth control</subject><subject>Germ cells</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Proto-Oncogene Proteins c-kit - biosynthesis</subject><subject>proto‐oncogenes</subject><subject>Spermatogonia - chemistry</subject><subject>Staining and Labeling</subject><subject>Stem Cell Factor - biosynthesis</subject><subject>stem cells</subject><subject>Sterility. Assisted procreation</subject><subject>testis</subject><subject>Testis - chemistry</subject><subject>Testis - metabolism</subject><issn>0196-3635</issn><issn>1939-4640</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkUFv1DAQhS0EKkvLT0CyEHBqtnbsdWxOVG2hhQou5Wx5nXHXKydZ7ETZ_vs6bLT3njz2vHnz9Bmhj5QsKSHlxXZJFVMFFzw_KCWW_ZpQScRy_wotjq3XaEGoEgUTbPUWvUtpm2cJrdgJOpFSqoqwBXI3-12ElHzX4s5hW_y6e8CmrbHvEw7-MZfnOPXQYAshYGds38Vz7FvcdrEx4b82DWsHsfcB8GZoTIt7SL23QzAR9z6lAc7QG2dCgvfzeYr-fr95uLot7v_8uLu6vC_siktRcMeAOlVJoGBqq0glS3CSEalKsMKaai1puaoNg3xhTgpCBNR2xYQVpSTsFH05-O5i92_IKXTj05TctNANSVeSV5kPzcKvB6GNXUoRnN5F35j4pCnRE2S91RNJPZHUE2Q9Q9b7PPxh3jKsG6iPozPV3P80902yJrhoWuvTUcZKXlLCs-zbQTZmck8vCKB_Xv6-nsps8flgsfGPm9FH0Cl_Ssi5qB7HkVaaa54TPQMDu6b6</recordid><startdate>199607</startdate><enddate>199607</enddate><creator>Sandlow, J. I</creator><creator>Feng, H. L</creator><creator>Cohen, M. B</creator><creator>Sandra, A</creator><general>Am Soc Andrology</general><general>Blackwell Publishing Ltd</general><general>American Society of Andrology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199607</creationdate><title>Expression of c-KIT and its ligand, stem cell factor, in normal and subfertile human testicular tissue</title><author>Sandlow, J. I ; Feng, H. L ; Cohen, M. B ; Sandra, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5486-4f3e1f978e1eadc90782ef830892ec6ca7b8125da3e6ca3f86006edc536c62803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Birth control</topic><topic>Germ cells</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Proto-Oncogene Proteins c-kit - biosynthesis</topic><topic>proto‐oncogenes</topic><topic>Spermatogonia - chemistry</topic><topic>Staining and Labeling</topic><topic>Stem Cell Factor - biosynthesis</topic><topic>stem cells</topic><topic>Sterility. Assisted procreation</topic><topic>testis</topic><topic>Testis - chemistry</topic><topic>Testis - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sandlow, J. I</creatorcontrib><creatorcontrib>Feng, H. L</creatorcontrib><creatorcontrib>Cohen, M. B</creatorcontrib><creatorcontrib>Sandra, A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of andrology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sandlow, J. I</au><au>Feng, H. L</au><au>Cohen, M. B</au><au>Sandra, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of c-KIT and its ligand, stem cell factor, in normal and subfertile human testicular tissue</atitle><jtitle>Journal of andrology</jtitle><addtitle>J Androl</addtitle><date>1996-07</date><risdate>1996</risdate><volume>17</volume><issue>4</issue><spage>403</spage><epage>408</epage><pages>403-408</pages><issn>0196-3635</issn><eissn>1939-4640</eissn><coden>JOAND3</coden><abstract>The c‐KIT proto‐oncogene encodes for a transmembrane receptor and is associated with maturation of several cell types, including germ cells. The ligand of the receptor has been identified as stem cell factor (SCF). Loss or alteration of the expression of either of these factors leads to anemia, albinism, and/or sterility in mice. We examined the expression of c‐KIT and SCF by immunohistochemistry in specimens from normal and infertile human testis. All specimens were obtained in the evaluation of male subfertility. We were able to demonstrate staining for c‐KIT in Leydig cells in all specimens. Normal testis stained for c‐KIT in the cytoplasm of early spermatogenic cells, as well as the acrosomal granules of the round spermatids and the acrosome of testicular spermatozoa. However, staining in testis demonstrating maturation arrest failed to demonstrate acrosomal staining, and Sertoli‐only specimens demonstrated staining for c‐KIT in Leydig cells only. The results for SCF demonstrated an overall uniform staining of Leydig cells in all specimens. The intensity of staining of Sertoli cells increased from normal to maturation arrest to Sertoli‐only specimens. Germ cell staining was consistently negative. We hypothesize that these staining patterns for SCF are due to either lack of staining of the receptor‐ligand complex or overexpression of the kit ligand in tissue that does not express the kit receptor. It appears that the c‐kit receptor is expressed in the acrosome of developing germ cells, as well as in Leydig cells and early spermatogenic cells, suggesting a role in the acrosome reaction, as well as germ cell maturation and differentiation.</abstract><cop>Oxford, UK</cop><pub>Am Soc Andrology</pub><pmid>8889703</pmid><doi>10.1002/j.1939-4640.1996.tb01806.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adult Biological and medical sciences Birth control Germ cells Gynecology. Andrology. Obstetrics Humans Immunohistochemistry Male Medical sciences Proto-Oncogene Proteins c-kit - biosynthesis proto‐oncogenes Spermatogonia - chemistry Staining and Labeling Stem Cell Factor - biosynthesis stem cells Sterility. Assisted procreation testis Testis - chemistry Testis - metabolism |
title | Expression of c-KIT and its ligand, stem cell factor, in normal and subfertile human testicular tissue |
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