Elevated tissue levels of collagenase during dilation of uterine cervix in human parturition
Seven biopsy specimens from the cervix and 17 from the lower uterine segment were obtained in 24 women at term (37 to 42 weeks). Collagenase was extracted and assayed on telopeptide-free [3H]collagen; typical collagen cleavage products were found on sodium dodecyl sulfate-gel electrophoresis. There...
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| Veröffentlicht in: | American journal of obstetrics and gynecology 1988-10, Vol.159 (4), p.971-976 |
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| container_title | American journal of obstetrics and gynecology |
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| creator | Rajabi, Mohammad R. Dean, David D. Beydoun, Samir N. Woessner, J. Frederick |
| description | Seven biopsy specimens from the cervix and 17 from the lower uterine segment were obtained in 24 women at term (37 to 42 weeks). Collagenase was extracted and assayed on telopeptide-free [3H]collagen; typical collagen cleavage products were found on sodium dodecyl sulfate-gel electrophoresis. There was no significant difference between collagenase levels in the cervix and in the lower uterine segment in women not in labor and with the cervix closed. Levels of active and latent collagenase in 11 such specimens were 0.14 ± 0.03 and 0.64 ± 0.90 U/gm wet weight, respectively (mean ± SEM; 1 U = 1 μg collagen digested per minute at 30° C). Thirteen women at term in active labor with cervical dilation of 4 to 8 cm exhibited a thirteenfold increase in mean collagenase activity in the lower uterine segment. Active and latent collagenase increased to 2.06 ± 0.92 and 8.64 ± 2.87 U/gm, respectively. This is the first direct evidence that interstitial collagenase increases markedly during cervical dilation in human parturition. |
| doi_str_mv | 10.1016/S0002-9378(88)80183-2 |
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Frederick</creator><creatorcontrib>Rajabi, Mohammad R. ; Dean, David D. ; Beydoun, Samir N. ; Woessner, J. Frederick</creatorcontrib><description>Seven biopsy specimens from the cervix and 17 from the lower uterine segment were obtained in 24 women at term (37 to 42 weeks). Collagenase was extracted and assayed on telopeptide-free [3H]collagen; typical collagen cleavage products were found on sodium dodecyl sulfate-gel electrophoresis. There was no significant difference between collagenase levels in the cervix and in the lower uterine segment in women not in labor and with the cervix closed. Levels of active and latent collagenase in 11 such specimens were 0.14 ± 0.03 and 0.64 ± 0.90 U/gm wet weight, respectively (mean ± SEM; 1 U = 1 μg collagen digested per minute at 30° C). Thirteen women at term in active labor with cervical dilation of 4 to 8 cm exhibited a thirteenfold increase in mean collagenase activity in the lower uterine segment. 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Frederick</creatorcontrib><title>Elevated tissue levels of collagenase during dilation of uterine cervix in human parturition</title><title>American journal of obstetrics and gynecology</title><addtitle>Am J Obstet Gynecol</addtitle><description>Seven biopsy specimens from the cervix and 17 from the lower uterine segment were obtained in 24 women at term (37 to 42 weeks). Collagenase was extracted and assayed on telopeptide-free [3H]collagen; typical collagen cleavage products were found on sodium dodecyl sulfate-gel electrophoresis. There was no significant difference between collagenase levels in the cervix and in the lower uterine segment in women not in labor and with the cervix closed. Levels of active and latent collagenase in 11 such specimens were 0.14 ± 0.03 and 0.64 ± 0.90 U/gm wet weight, respectively (mean ± SEM; 1 U = 1 μg collagen digested per minute at 30° C). Thirteen women at term in active labor with cervical dilation of 4 to 8 cm exhibited a thirteenfold increase in mean collagenase activity in the lower uterine segment. Active and latent collagenase increased to 2.06 ± 0.92 and 8.64 ± 2.87 U/gm, respectively. This is the first direct evidence that interstitial collagenase increases markedly during cervical dilation in human parturition.</description><subject>Biological and medical sciences</subject><subject>Cervix</subject><subject>Cervix Uteri - enzymology</subject><subject>Cervix Uteri - physiology</subject><subject>Chromatography, Gel</subject><subject>collagen</subject><subject>collagenase</subject><subject>dilation of cervix</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hormone metabolism and regulation</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>labor</subject><subject>Labor, Obstetric - metabolism</subject><subject>Labor, Obstetric - physiology</subject><subject>Microbial Collagenase - metabolism</subject><subject>Pregnancy</subject><subject>Pregnancy. Parturition. Lactation</subject><subject>Uterus - enzymology</subject><subject>Vertebrates: reproduction</subject><issn>0002-9378</issn><issn>1097-6868</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtKxDAUhoMo43h5hIEsRHRRzaVN0pWIeIMBF-pOCGlyqpFOOybtoG9v6gyzFQLh5Hzn5OdDaEbJBSVUXD4TQlhWcqnOlDpXhCqesR00paSUmVBC7aLpFtlHBzF-jiUr2QRNmMoLqcQUvd02sDI9ONz7GAfAqYQm4q7Gtmsa8w6tiYDdEHz7jp1vTO-7dmwPPaQ3wBbCyn9j3-KPYWFavDShT_SIHaG92jQRjjf3IXq9u325ecjmT_ePN9fzzHJV9lldu4oo6gpX0Lyqa2G4tLbiUBImgRPLeMkFzSUhXFY5V1TwQjJqIRclNZYfotP13mXovgaIvV74aCHFb6EbopYql1KUMoHFGrShizFArZfBL0z40ZTo0ar-s6pHZVqlM1rVLM3NNh8M1QLcdmqjMfVPNn0TrWnqYFrr4xYTKSwl45qrNZYMw8pD0NF6aC04H8D22nX-nyC_or6USA</recordid><startdate>19881001</startdate><enddate>19881001</enddate><creator>Rajabi, Mohammad R.</creator><creator>Dean, David D.</creator><creator>Beydoun, Samir N.</creator><creator>Woessner, J. Frederick</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19881001</creationdate><title>Elevated tissue levels of collagenase during dilation of uterine cervix in human parturition</title><author>Rajabi, Mohammad R. ; Dean, David D. ; Beydoun, Samir N. ; Woessner, J. Frederick</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-ffdb081d5d514bff6a37ccb3e9027e30c239361470037b4381635721ce4691ac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Biological and medical sciences</topic><topic>Cervix</topic><topic>Cervix Uteri - enzymology</topic><topic>Cervix Uteri - physiology</topic><topic>Chromatography, Gel</topic><topic>collagen</topic><topic>collagenase</topic><topic>dilation of cervix</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hormone metabolism and regulation</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>labor</topic><topic>Labor, Obstetric - metabolism</topic><topic>Labor, Obstetric - physiology</topic><topic>Microbial Collagenase - metabolism</topic><topic>Pregnancy</topic><topic>Pregnancy. Parturition. Lactation</topic><topic>Uterus - enzymology</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rajabi, Mohammad R.</creatorcontrib><creatorcontrib>Dean, David D.</creatorcontrib><creatorcontrib>Beydoun, Samir N.</creatorcontrib><creatorcontrib>Woessner, J. Frederick</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of obstetrics and gynecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rajabi, Mohammad R.</au><au>Dean, David D.</au><au>Beydoun, Samir N.</au><au>Woessner, J. Frederick</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Elevated tissue levels of collagenase during dilation of uterine cervix in human parturition</atitle><jtitle>American journal of obstetrics and gynecology</jtitle><addtitle>Am J Obstet Gynecol</addtitle><date>1988-10-01</date><risdate>1988</risdate><volume>159</volume><issue>4</issue><spage>971</spage><epage>976</epage><pages>971-976</pages><issn>0002-9378</issn><eissn>1097-6868</eissn><coden>AJOGAH</coden><abstract>Seven biopsy specimens from the cervix and 17 from the lower uterine segment were obtained in 24 women at term (37 to 42 weeks). Collagenase was extracted and assayed on telopeptide-free [3H]collagen; typical collagen cleavage products were found on sodium dodecyl sulfate-gel electrophoresis. There was no significant difference between collagenase levels in the cervix and in the lower uterine segment in women not in labor and with the cervix closed. Levels of active and latent collagenase in 11 such specimens were 0.14 ± 0.03 and 0.64 ± 0.90 U/gm wet weight, respectively (mean ± SEM; 1 U = 1 μg collagen digested per minute at 30° C). Thirteen women at term in active labor with cervical dilation of 4 to 8 cm exhibited a thirteenfold increase in mean collagenase activity in the lower uterine segment. Active and latent collagenase increased to 2.06 ± 0.92 and 8.64 ± 2.87 U/gm, respectively. This is the first direct evidence that interstitial collagenase increases markedly during cervical dilation in human parturition.</abstract><cop>Philadelphia, PA</cop><pub>Elsevier Inc</pub><pmid>2845786</pmid><doi>10.1016/S0002-9378(88)80183-2</doi><tpages>6</tpages></addata></record> |
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| subjects | Biological and medical sciences Cervix Cervix Uteri - enzymology Cervix Uteri - physiology Chromatography, Gel collagen collagenase dilation of cervix Electrophoresis, Polyacrylamide Gel Female Fundamental and applied biological sciences. Psychology Hormone metabolism and regulation Humans In Vitro Techniques labor Labor, Obstetric - metabolism Labor, Obstetric - physiology Microbial Collagenase - metabolism Pregnancy Pregnancy. Parturition. Lactation Uterus - enzymology Vertebrates: reproduction |
| title | Elevated tissue levels of collagenase during dilation of uterine cervix in human parturition |
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