Refinement of the differentiated phenotype of the spermatogenic cell line GC-2spd(ts)

A transformed spermatogenic cell line GC-2spd(ts), recently reported to express a protein marker of spermiogenesis, was tested for the presence of several mRNAs encoded by genes transcribed specifically in the testis and at precise stages of spermatogenesis. Northern blotting and reverse-transcripta...

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Veröffentlicht in:	Biology of reproduction 1996-10, Vol.55 (4), p.923-932
Hauptverfasser:	WOLKOWICZ, M. J, COONROD, S. M, PRABHAKARA REDDI, P, MILLAN, J. L, HOFMANN, M.-C, HERR, J. C
Format:	Artikel
Sprache:	eng
Schlagworte:	Acrosin - analysis Acrosin - genetics Acrosome Animal cells Animals Antigens Antigens, Differentiation - analysis Antigens, Differentiation - genetics Antigens, Differentiation - immunology Antigens, Surface - analysis Antigens, Surface - genetics Biological and medical sciences Biotechnology Blotting, Northern Cell Differentiation - genetics Cell Differentiation - immunology Cell Differentiation - physiology Cell Line, Transformed Electrophoresis, Polyacrylamide Gel Eukaryotic cell cultures Flow Cytometry Fundamental and applied biological sciences. Psychology Genetical aspects Gonadal Steroid Hormones Humans Immunoblotting Isoenzymes L-Lactate Dehydrogenase - analysis L-Lactate Dehydrogenase - genetics Male Membrane Proteins Methods. Procedures. Technologies Mice Phenotype Polymerase Chain Reaction Protamines - analysis Protamines - genetics Proteins - analysis Proteins - genetics RNA - analysis RNA - genetics Spermatocytes - cytology Spermatocytes - physiology Spermatogenesis - physiology Temperature Time Factors
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