Electrofusion parameters for nuclear transfer predicted using isofusion contours produced with bovine embryonic cells
Electrofusion is a valuable technique for the nuclear transfer procedure. An enucleated oocyte is electrofused with a blastomere to create a nuclear transfer embryo. The present study constructed isofusion contours after the electrofusion of identical coupled cells that characterized all the bovine...
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| Veröffentlicht in: | Molecular reproduction and development 1996-03, Vol.43 (3), p.306-312 |
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| container_title | Molecular reproduction and development |
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| creator | Tatham, B.G. (Monash University, Clayton, Victoria, Australia.) Giliam, K.J Trounson, A.O |
| description | Electrofusion is a valuable technique for the nuclear transfer procedure. An enucleated oocyte is electrofused with a blastomere to create a nuclear transfer embryo. The present study constructed isofusion contours after the electrofusion of identical coupled cells that characterized all the bovine embryonic cell types used in nuclear transfer. The intersection of isofusion contours for enucleated oocytes and blastomeres provided the parameters for electrofusion during nuclear transfer. Blastomeres isolated from in vitro produced embryos 3–6 days after (in vitro fertilization) were electrofused with oocytes enucleated by centrifugation (85, 87, 89, and 73% electrofusion, respectively). The cleavage (46, 40, 37, and 28%, respectively) of the nuclear transfer embryos produced a trend that decreased as the age of the blastomeres increased. The isofusion contours provided information about the interaction between different cell types in an electric field, and gave precise electrofusion parameters for a range of bovine embryonic cell types used in nuclear transfer. © 1996 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/(SICI)1098-2795(199603)43:3<306::AID-MRD4>3.0.CO;2-U |
| format | Article |
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(Monash University, Clayton, Victoria, Australia.) ; Giliam, K.J ; Trounson, A.O</creator><creatorcontrib>Tatham, B.G. (Monash University, Clayton, Victoria, Australia.) ; Giliam, K.J ; Trounson, A.O</creatorcontrib><description>Electrofusion is a valuable technique for the nuclear transfer procedure. An enucleated oocyte is electrofused with a blastomere to create a nuclear transfer embryo. The present study constructed isofusion contours after the electrofusion of identical coupled cells that characterized all the bovine embryonic cell types used in nuclear transfer. The intersection of isofusion contours for enucleated oocytes and blastomeres provided the parameters for electrofusion during nuclear transfer. Blastomeres isolated from in vitro produced embryos 3–6 days after (in vitro fertilization) were electrofused with oocytes enucleated by centrifugation (85, 87, 89, and 73% electrofusion, respectively). The cleavage (46, 40, 37, and 28%, respectively) of the nuclear transfer embryos produced a trend that decreased as the age of the blastomeres increased. 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Teratology ; EMBRYONIC DEVELOPMENT ; Fundamental and applied biological sciences. 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(Monash University, Clayton, Victoria, Australia.)</creatorcontrib><creatorcontrib>Giliam, K.J</creatorcontrib><creatorcontrib>Trounson, A.O</creatorcontrib><title>Electrofusion parameters for nuclear transfer predicted using isofusion contours produced with bovine embryonic cells</title><title>Molecular reproduction and development</title><addtitle>Mol. Reprod. Dev</addtitle><description>Electrofusion is a valuable technique for the nuclear transfer procedure. An enucleated oocyte is electrofused with a blastomere to create a nuclear transfer embryo. The present study constructed isofusion contours after the electrofusion of identical coupled cells that characterized all the bovine embryonic cell types used in nuclear transfer. The intersection of isofusion contours for enucleated oocytes and blastomeres provided the parameters for electrofusion during nuclear transfer. Blastomeres isolated from in vitro produced embryos 3–6 days after (in vitro fertilization) were electrofused with oocytes enucleated by centrifugation (85, 87, 89, and 73% electrofusion, respectively). The cleavage (46, 40, 37, and 28%, respectively) of the nuclear transfer embryos produced a trend that decreased as the age of the blastomeres increased. The isofusion contours provided information about the interaction between different cell types in an electric field, and gave precise electrofusion parameters for a range of bovine embryonic cell types used in nuclear transfer. © 1996 Wiley‐Liss, Inc.</description><subject>AGE</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>BLASTOMERE</subject><subject>Blastomeres - physiology</subject><subject>BOVIN</subject><subject>bovine</subject><subject>CATTLE</subject><subject>Cell Fusion</subject><subject>Cytological Techniques</subject><subject>DESARROLLO EMBRIONARIO</subject><subject>DEVELOPPEMENT EMBRYONNAIRE</subject><subject>Early stages. Segmentation. Gastrulation. Neurulation</subject><subject>EDAD</subject><subject>electrofusion</subject><subject>embryo</subject><subject>EMBRYO AGE</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>EMBRYONIC DEVELOPMENT</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GANADO BOVINO</subject><subject>NOYAU CELLULAIRE</subject><subject>nuclear transfer</subject><subject>Nuclear Transfer Techniques</subject><subject>NUCLEI</subject><subject>NUCLEO</subject><subject>NUCLEUS</subject><subject>OOCYTES</subject><subject>OVA</subject><subject>OVULE</subject><subject>OVULO</subject><issn>1040-452X</issn><issn>1098-2795</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV-L1DAUxYso67r6BQShDyK7Dx3TJE3bcRGW7h8HVgfcHVd8uaTpzRrtNGPSus63N3XG8UFBQklCzj39cU4UHadkkhJCXx5ezarZUUrKIqF5mR2mZSkIO-Jsyo4ZEdPpyew0efv-lL9mEzKp5q9osrgX7e8G7o9nThKe0Y8Po0fefyGElGVB9qK9ohAF5Ww_Gs5aVL2zevDGdvFKOrnEHp2PtXVxN6gWpYt7Jzuv0cUrh41RPTZx0He3sfG_J5XtejuEuZWzzaCC4s70n-Pafjcdxris3dp2RsUK29Y_jh5o2Xp8st0PosX52XX1JrmcX8yqk8tEcS54wpQWlIgmzVIUmuZYNzTnWV0LnsmSYsaKgos0bzQWGdPIuNAYPqHLmivdsIPoxcY3QH0b0PewNH4kkB3awUNe8JBOxoLweiNUznrvUMPKmaV0a0gJjG0AjG3AGC6M4cKmDeAMwiICILQBYxvhTqCaA4VFsH22_f9QL7HZmW7jD-_Pt-_SK9nqELMyfidjJE1zWv6huzMtrv9C-w_ZP8B-3YNtsrE1vscfO1vpvoLIWZ7BzbsLyD-QT-f0_AaqoH-60WtpQd66QLq4KnOeisD4E5nFzAY</recordid><startdate>199603</startdate><enddate>199603</enddate><creator>Tatham, B.G. (Monash University, Clayton, Victoria, Australia.)</creator><creator>Giliam, K.J</creator><creator>Trounson, A.O</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199603</creationdate><title>Electrofusion parameters for nuclear transfer predicted using isofusion contours produced with bovine embryonic cells</title><author>Tatham, B.G. (Monash University, Clayton, Victoria, Australia.) ; Giliam, K.J ; Trounson, A.O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4464-3cf6206d151e6f27ebd2745bb645a92e53884617dfe853fe346fe46f6f9b4cfd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>AGE</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>BLASTOMERE</topic><topic>Blastomeres - physiology</topic><topic>BOVIN</topic><topic>bovine</topic><topic>CATTLE</topic><topic>Cell Fusion</topic><topic>Cytological Techniques</topic><topic>DESARROLLO EMBRIONARIO</topic><topic>DEVELOPPEMENT EMBRYONNAIRE</topic><topic>Early stages. Segmentation. Gastrulation. Neurulation</topic><topic>EDAD</topic><topic>electrofusion</topic><topic>embryo</topic><topic>EMBRYO AGE</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>EMBRYONIC DEVELOPMENT</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GANADO BOVINO</topic><topic>NOYAU CELLULAIRE</topic><topic>nuclear transfer</topic><topic>Nuclear Transfer Techniques</topic><topic>NUCLEI</topic><topic>NUCLEO</topic><topic>NUCLEUS</topic><topic>OOCYTES</topic><topic>OVA</topic><topic>OVULE</topic><topic>OVULO</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tatham, B.G. 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(Monash University, Clayton, Victoria, Australia.)</au><au>Giliam, K.J</au><au>Trounson, A.O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrofusion parameters for nuclear transfer predicted using isofusion contours produced with bovine embryonic cells</atitle><jtitle>Molecular reproduction and development</jtitle><addtitle>Mol. Reprod. Dev</addtitle><date>1996-03</date><risdate>1996</risdate><volume>43</volume><issue>3</issue><spage>306</spage><epage>312</epage><pages>306-312</pages><issn>1040-452X</issn><eissn>1098-2795</eissn><coden>MREDEE</coden><abstract>Electrofusion is a valuable technique for the nuclear transfer procedure. An enucleated oocyte is electrofused with a blastomere to create a nuclear transfer embryo. The present study constructed isofusion contours after the electrofusion of identical coupled cells that characterized all the bovine embryonic cell types used in nuclear transfer. The intersection of isofusion contours for enucleated oocytes and blastomeres provided the parameters for electrofusion during nuclear transfer. Blastomeres isolated from in vitro produced embryos 3–6 days after (in vitro fertilization) were electrofused with oocytes enucleated by centrifugation (85, 87, 89, and 73% electrofusion, respectively). The cleavage (46, 40, 37, and 28%, respectively) of the nuclear transfer embryos produced a trend that decreased as the age of the blastomeres increased. The isofusion contours provided information about the interaction between different cell types in an electric field, and gave precise electrofusion parameters for a range of bovine embryonic cell types used in nuclear transfer. © 1996 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>8868243</pmid><doi>10.1002/(SICI)1098-2795(199603)43:3<306::AID-MRD4>3.0.CO;2-U</doi><tpages>7</tpages></addata></record> |
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| ispartof | Molecular reproduction and development, 1996-03, Vol.43 (3), p.306-312 |
| issn | 1040-452X 1098-2795 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | AGE Animals Biological and medical sciences BLASTOMERE Blastomeres - physiology BOVIN bovine CATTLE Cell Fusion Cytological Techniques DESARROLLO EMBRIONARIO DEVELOPPEMENT EMBRYONNAIRE Early stages. Segmentation. Gastrulation. Neurulation EDAD electrofusion embryo EMBRYO AGE Embryology: invertebrates and vertebrates. Teratology EMBRYONIC DEVELOPMENT Fundamental and applied biological sciences. Psychology GANADO BOVINO NOYAU CELLULAIRE nuclear transfer Nuclear Transfer Techniques NUCLEI NUCLEO NUCLEUS OOCYTES OVA OVULE OVULO |
| title | Electrofusion parameters for nuclear transfer predicted using isofusion contours produced with bovine embryonic cells |
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