IFN-γ facilitates NGF-induced neuronal differentiation in PC12 cells
Natural or recombinant murine interferon-γ causes a reversible arrest of proliferation of PC12 cells. Treatment with other antimitotics (AraC, colchicine, mitomycin C, hydroxy-urea) or removal of serum, on the contrary, leads to mitotic arrest followed by cell death. IFN-γ-treated PC12 cells respond...
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| Veröffentlicht in: | Experimental cell research 1988-11, Vol.179 (1), p.1-9 |
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| creator | Improta, T. Salvatore, A.M. Di Luzio, A. Romeo, G. Coccia, E.M. Calissano, P. |
| description | Natural or recombinant murine interferon-γ causes a reversible arrest of proliferation of PC12 cells. Treatment with other antimitotics (AraC, colchicine, mitomycin C, hydroxy-urea) or removal of serum, on the contrary, leads to mitotic arrest followed by cell death. IFN-γ-treated PC12 cells respond more rapidly to NGF in terms of speed of neuronal outgrowth. On the other hand, NGF potentiates the action of IFN-γ in stimulating the enzyme 2′,5′-A synthetase which shifts from an average of 4.4-fold stimulation at 48 h with IFN-γ alone to increments varying between 5- and 18-fold when PC12 cells are treated for 48 h with IFN-γ and NGF. NGF alone, on the contrary, does not exert any detectable effect on this enzyme. From these findings we propose the use of a combined treatment of PC12 cells with NGF and IFN-γ for a more rapid induction of neuronal differentiation. |
| doi_str_mv | 10.1016/0014-4827(88)90342-4 |
| format | Article |
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Treatment with other antimitotics (AraC, colchicine, mitomycin C, hydroxy-urea) or removal of serum, on the contrary, leads to mitotic arrest followed by cell death. IFN-γ-treated PC12 cells respond more rapidly to NGF in terms of speed of neuronal outgrowth. On the other hand, NGF potentiates the action of IFN-γ in stimulating the enzyme 2′,5′-A synthetase which shifts from an average of 4.4-fold stimulation at 48 h with IFN-γ alone to increments varying between 5- and 18-fold when PC12 cells are treated for 48 h with IFN-γ and NGF. NGF alone, on the contrary, does not exert any detectable effect on this enzyme. 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Psychology ; Hydroxyurea - pharmacology ; Interferon-gamma - pharmacology ; Mice ; Mitomycin ; Mitomycins - pharmacology ; Molecular and cellular biology ; Nerve Growth Factors - pharmacology ; Neurons - cytology ; Neurons - drug effects ; Pheochromocytoma ; Rats ; Recombinant Proteins - pharmacology ; Responses to growth factors, tumor promotors, other factors ; Tumor Cells, Cultured</subject><ispartof>Experimental cell research, 1988-11, Vol.179 (1), p.1-9</ispartof><rights>1988</rights><rights>1989 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-6c55948dee4883106209b5046b2c618cb0aa8eb147c7dd14b02ce3ceb3cf18c83</citedby><cites>FETCH-LOGICAL-c417t-6c55948dee4883106209b5046b2c618cb0aa8eb147c7dd14b02ce3ceb3cf18c83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014482788903424$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7153522$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3139434$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Improta, T.</creatorcontrib><creatorcontrib>Salvatore, A.M.</creatorcontrib><creatorcontrib>Di Luzio, A.</creatorcontrib><creatorcontrib>Romeo, G.</creatorcontrib><creatorcontrib>Coccia, E.M.</creatorcontrib><creatorcontrib>Calissano, P.</creatorcontrib><title>IFN-γ facilitates NGF-induced neuronal differentiation in PC12 cells</title><title>Experimental cell research</title><addtitle>Exp Cell Res</addtitle><description>Natural or recombinant murine interferon-γ causes a reversible arrest of proliferation of PC12 cells. Treatment with other antimitotics (AraC, colchicine, mitomycin C, hydroxy-urea) or removal of serum, on the contrary, leads to mitotic arrest followed by cell death. IFN-γ-treated PC12 cells respond more rapidly to NGF in terms of speed of neuronal outgrowth. On the other hand, NGF potentiates the action of IFN-γ in stimulating the enzyme 2′,5′-A synthetase which shifts from an average of 4.4-fold stimulation at 48 h with IFN-γ alone to increments varying between 5- and 18-fold when PC12 cells are treated for 48 h with IFN-γ and NGF. NGF alone, on the contrary, does not exert any detectable effect on this enzyme. From these findings we propose the use of a combined treatment of PC12 cells with NGF and IFN-γ for a more rapid induction of neuronal differentiation.</description><subject>Adrenal Gland Neoplasms</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell physiology</subject><subject>Cell Survival - drug effects</subject><subject>Colchicine - pharmacology</subject><subject>Cytarabine - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydroxyurea - pharmacology</subject><subject>Interferon-gamma - pharmacology</subject><subject>Mice</subject><subject>Mitomycin</subject><subject>Mitomycins - pharmacology</subject><subject>Molecular and cellular biology</subject><subject>Nerve Growth Factors - pharmacology</subject><subject>Neurons - cytology</subject><subject>Neurons - drug effects</subject><subject>Pheochromocytoma</subject><subject>Rats</subject><subject>Recombinant Proteins - pharmacology</subject><subject>Responses to growth factors, tumor promotors, other factors</subject><subject>Tumor Cells, Cultured</subject><issn>0014-4827</issn><issn>1090-2422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1KxDAUhYMoOv68gUIXIrqo5ia3bboRZHBUEHWh65CmtxDppJq0gs_le_hMdpxhlrq6i_Odw-Vj7BD4OXDILzgHTFGJ4lSps5JLFClusAnwkqcChdhkkzWyw3ZjfOWcKwX5NtuWIEuUOGHXd7OH9PsraYx1retNTzF5uJmlzteDpTrxNITOmzapXdNQIN8707vOJ84nT1MQiaW2jftsqzFtpIPV3WMvs-vn6W16_3hzN726Ty1C0ae5zbISVU2ESkngueBllXHMK2FzULbixiiqAAtb1DVgxYUlaamSthljJffYyXL3LXTvA8Vez11cfGA8dUPUhUKUkJf_gpAJKcpfEJegDV2MgRr9FtzchE8NXC8064VDvXColdK_mjWOtaPV_lDNqV6XVl7H_HiVm2hN2wTjrYtrrIBMZkKM2OUSo1Hah6Ogo3XkR_EukO113bm___gB9jWXSQ</recordid><startdate>19881101</startdate><enddate>19881101</enddate><creator>Improta, T.</creator><creator>Salvatore, A.M.</creator><creator>Di Luzio, A.</creator><creator>Romeo, G.</creator><creator>Coccia, E.M.</creator><creator>Calissano, P.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19881101</creationdate><title>IFN-γ facilitates NGF-induced neuronal differentiation in PC12 cells</title><author>Improta, T. ; Salvatore, A.M. ; Di Luzio, A. ; Romeo, G. ; Coccia, E.M. ; Calissano, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-6c55948dee4883106209b5046b2c618cb0aa8eb147c7dd14b02ce3ceb3cf18c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Adrenal Gland Neoplasms</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell physiology</topic><topic>Cell Survival - drug effects</topic><topic>Colchicine - pharmacology</topic><topic>Cytarabine - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydroxyurea - pharmacology</topic><topic>Interferon-gamma - pharmacology</topic><topic>Mice</topic><topic>Mitomycin</topic><topic>Mitomycins - pharmacology</topic><topic>Molecular and cellular biology</topic><topic>Nerve Growth Factors - pharmacology</topic><topic>Neurons - cytology</topic><topic>Neurons - drug effects</topic><topic>Pheochromocytoma</topic><topic>Rats</topic><topic>Recombinant Proteins - pharmacology</topic><topic>Responses to growth factors, tumor promotors, other factors</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Improta, T.</creatorcontrib><creatorcontrib>Salvatore, A.M.</creatorcontrib><creatorcontrib>Di Luzio, A.</creatorcontrib><creatorcontrib>Romeo, G.</creatorcontrib><creatorcontrib>Coccia, E.M.</creatorcontrib><creatorcontrib>Calissano, P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Improta, T.</au><au>Salvatore, A.M.</au><au>Di Luzio, A.</au><au>Romeo, G.</au><au>Coccia, E.M.</au><au>Calissano, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>IFN-γ facilitates NGF-induced neuronal differentiation in PC12 cells</atitle><jtitle>Experimental cell research</jtitle><addtitle>Exp Cell Res</addtitle><date>1988-11-01</date><risdate>1988</risdate><volume>179</volume><issue>1</issue><spage>1</spage><epage>9</epage><pages>1-9</pages><issn>0014-4827</issn><eissn>1090-2422</eissn><coden>ECREAL</coden><abstract>Natural or recombinant murine interferon-γ causes a reversible arrest of proliferation of PC12 cells. Treatment with other antimitotics (AraC, colchicine, mitomycin C, hydroxy-urea) or removal of serum, on the contrary, leads to mitotic arrest followed by cell death. IFN-γ-treated PC12 cells respond more rapidly to NGF in terms of speed of neuronal outgrowth. On the other hand, NGF potentiates the action of IFN-γ in stimulating the enzyme 2′,5′-A synthetase which shifts from an average of 4.4-fold stimulation at 48 h with IFN-γ alone to increments varying between 5- and 18-fold when PC12 cells are treated for 48 h with IFN-γ and NGF. NGF alone, on the contrary, does not exert any detectable effect on this enzyme. From these findings we propose the use of a combined treatment of PC12 cells with NGF and IFN-γ for a more rapid induction of neuronal differentiation.</abstract><cop>Orlando, FL</cop><pub>Elsevier Inc</pub><pmid>3139434</pmid><doi>10.1016/0014-4827(88)90342-4</doi><tpages>9</tpages></addata></record> |
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| subjects | Adrenal Gland Neoplasms Animals Biological and medical sciences Cell Differentiation - drug effects Cell physiology Cell Survival - drug effects Colchicine - pharmacology Cytarabine - pharmacology Fundamental and applied biological sciences. Psychology Hydroxyurea - pharmacology Interferon-gamma - pharmacology Mice Mitomycin Mitomycins - pharmacology Molecular and cellular biology Nerve Growth Factors - pharmacology Neurons - cytology Neurons - drug effects Pheochromocytoma Rats Recombinant Proteins - pharmacology Responses to growth factors, tumor promotors, other factors Tumor Cells, Cultured |
| title | IFN-γ facilitates NGF-induced neuronal differentiation in PC12 cells |
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