Preferential Release of ATP and Its Extracellular Catabolism as a Source of Adenosine upon High‐ but Not Low‐Frequency Stimulation of Rat Hippocampal Slices

: The release of adenosine and ATP evoked by electrical field stimulation in rat hippocampal slices was investigated with the following two patterns of stimulation: (1) a brief, high‐frequency burst stimulation (trains of stimuli at 100 Hz for 50 ms applied every 2 s for 1 min), to mimic a long‐term...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of neurochemistry 1996-11, Vol.67 (5), p.2180-2187
Hauptverfasser: Cunha, Rodrigo A., Vizi, E. Sylvester, Ribeiro, J. Alexandre, Sebastião, Ana M.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 2187
container_issue 5
container_start_page 2180
container_title Journal of neurochemistry
container_volume 67
creator Cunha, Rodrigo A.
Vizi, E. Sylvester
Ribeiro, J. Alexandre
Sebastião, Ana M.
description : The release of adenosine and ATP evoked by electrical field stimulation in rat hippocampal slices was investigated with the following two patterns of stimulation: (1) a brief, high‐frequency burst stimulation (trains of stimuli at 100 Hz for 50 ms applied every 2 s for 1 min), to mimic a long‐term potentiation (LTP) stimulation paradigm, and (2) a more prolonged (3 min) and low‐frequency (5 Hz) train stimulation, to mimic a long‐term depression (LTD) stimulation paradigm. The release of ATP was greater at a brief, high‐frequency burst stimulation, whereas the release of [3H]adenosine was slightly greater at a more prolonged and low‐frequency stimulation. To investigate the source of extracellular adenosine, the following two pharmacological tools were used; α,β‐methylene ADP (AOPCP), an inhibitor of ecto‐5′‐nucleotidase, to assess the contribution of the catabolism of released adenine nucleotides as a source of extracellular adenosine, and S‐(4‐nitrobenzyl)‐6‐thioinosine (NBTI), an inhibitor of adenosine transporters, to assess the contribution of the release of adenosine, as such, as a source of extracellular adenosine. At low‐frequency stimulation, NBTI inhibited by nearly 50% the evoked outflow of [3H]adenosine, whereas AOPCP inhibited [3H]adenosine outflow only marginally. In contrast, at high‐frequency stimulation, AOPCP inhibited by 30% the evoked release of [3H]adenosine, whereas NBTI produced a 40% inhibition of [3H]adenosine outflow. At both frequencies, the kinetics of evoked [3H]adenosine outflow was affected in different manners by AOPCP and NBTI; NBTI mainly depressed the rate of evoked [3H]adenosine outflow, whereas AOPCP mainly inhibited the later phase of evoked [3H]adenosine accumulation. These results show that there is a simultaneous, but quantitatively different, release of ATP and adenosine from rat hippocampal slices stimulated at frequencies that can induce plasticity phenomena such as LTP (100 Hz) or LTD (5 Hz). The source of extracellular adenosine is also different according to the frequency of stimulation; i.e., at a brief, high‐frequency stimulation there is a greater contribution of released adenine nucleotides for the formation of extracellular adenosine than at a low frequency with a more prolonged stimulation.
doi_str_mv 10.1046/j.1471-4159.1996.67052180.x
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_78440236</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>78440236</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4370-ed6ba36d32638825e69ee1315410dc51290b3a069ed089f18eecdf4e20858c253</originalsourceid><addsrcrecordid>eNqVkd9u0zAUxiMEGmXwCEiWQNwl-H8dcTWVjQ1V27SOa8t1TsBVEgc70do7HoFH4Nl4Ehy16y3alXX8_b5zjv1l2TuCC4K5_LgpCJ-TnBNRFqQsZSHnWFCicLF9ls2O2vNshjGlOcOcvsxexbjBmEguyUl2opRkgpaz7M9tgBoCdIMzDbqDBkwE5Gt0dn-LTFehqyGi8-0QjIWmGRsT0MIMZu0bF1tkIjJo5cdg954KOh9dB2jsfYcu3fcff3_9RutxQNd-QEv_kMqLAD9H6OwOrQbXpo6DS2xy35khWfreW9P2aZlV4yzE19mL2jQR3hzO0-zbxfn94jJf3ny5Wpwtc8vZHOdQybVhsmJUMqWoAFkCEEYEJ7iygtASr5nB6bbCqqyJArBVzYFiJZSlgp1mH_Z9--DTfnHQrYvTm00Hfox6rjjHlMn_gkSokoqSJvDTHrTBx5i-WffBtSbsNMF6ClJv9BSWnsLSU5D6MUi9Te63hzHjuoXq6D0kl_T3B91Ea5o6mM66eMQYFYorkrDPe-zBNbB7ygb66_XisWL_AL8nvWk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15892592</pqid></control><display><type>article</type><title>Preferential Release of ATP and Its Extracellular Catabolism as a Source of Adenosine upon High‐ but Not Low‐Frequency Stimulation of Rat Hippocampal Slices</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Cunha, Rodrigo A. ; Vizi, E. Sylvester ; Ribeiro, J. Alexandre ; Sebastião, Ana M.</creator><creatorcontrib>Cunha, Rodrigo A. ; Vizi, E. Sylvester ; Ribeiro, J. Alexandre ; Sebastião, Ana M.</creatorcontrib><description>: The release of adenosine and ATP evoked by electrical field stimulation in rat hippocampal slices was investigated with the following two patterns of stimulation: (1) a brief, high‐frequency burst stimulation (trains of stimuli at 100 Hz for 50 ms applied every 2 s for 1 min), to mimic a long‐term potentiation (LTP) stimulation paradigm, and (2) a more prolonged (3 min) and low‐frequency (5 Hz) train stimulation, to mimic a long‐term depression (LTD) stimulation paradigm. The release of ATP was greater at a brief, high‐frequency burst stimulation, whereas the release of [3H]adenosine was slightly greater at a more prolonged and low‐frequency stimulation. To investigate the source of extracellular adenosine, the following two pharmacological tools were used; α,β‐methylene ADP (AOPCP), an inhibitor of ecto‐5′‐nucleotidase, to assess the contribution of the catabolism of released adenine nucleotides as a source of extracellular adenosine, and S‐(4‐nitrobenzyl)‐6‐thioinosine (NBTI), an inhibitor of adenosine transporters, to assess the contribution of the release of adenosine, as such, as a source of extracellular adenosine. At low‐frequency stimulation, NBTI inhibited by nearly 50% the evoked outflow of [3H]adenosine, whereas AOPCP inhibited [3H]adenosine outflow only marginally. In contrast, at high‐frequency stimulation, AOPCP inhibited by 30% the evoked release of [3H]adenosine, whereas NBTI produced a 40% inhibition of [3H]adenosine outflow. At both frequencies, the kinetics of evoked [3H]adenosine outflow was affected in different manners by AOPCP and NBTI; NBTI mainly depressed the rate of evoked [3H]adenosine outflow, whereas AOPCP mainly inhibited the later phase of evoked [3H]adenosine accumulation. These results show that there is a simultaneous, but quantitatively different, release of ATP and adenosine from rat hippocampal slices stimulated at frequencies that can induce plasticity phenomena such as LTP (100 Hz) or LTD (5 Hz). The source of extracellular adenosine is also different according to the frequency of stimulation; i.e., at a brief, high‐frequency stimulation there is a greater contribution of released adenine nucleotides for the formation of extracellular adenosine than at a low frequency with a more prolonged stimulation.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1046/j.1471-4159.1996.67052180.x</identifier><identifier>PMID: 8863529</identifier><identifier>CODEN: JONRA9</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>5'-Nucleotidase - antagonists &amp; inhibitors ; Adenosine ; Adenosine - metabolism ; Adenosine Diphosphate - analogs &amp; derivatives ; Adenosine Diphosphate - pharmacology ; Adenosine Triphosphate - metabolism ; Animals ; ATP ; Biochemistry and metabolism ; Biological and medical sciences ; Central nervous system ; Ectonucleotidases ; Electric Stimulation ; Energy Metabolism ; Enzyme Inhibitors - pharmacology ; Fundamental and applied biological sciences. Psychology ; Hippocampus ; Hippocampus - metabolism ; Hippocampus - physiology ; In Vitro Techniques ; Kinetics ; Long-Term Potentiation ; Male ; Models, Neurological ; Neurons - cytology ; Neurons - metabolism ; Neurons - physiology ; Rats ; Rats, Wistar ; Synaptic plasticity ; Time Factors ; Tritium ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of neurochemistry, 1996-11, Vol.67 (5), p.2180-2187</ispartof><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4370-ed6ba36d32638825e69ee1315410dc51290b3a069ed089f18eecdf4e20858c253</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1471-4159.1996.67052180.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1471-4159.1996.67052180.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=3258481$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8863529$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cunha, Rodrigo A.</creatorcontrib><creatorcontrib>Vizi, E. Sylvester</creatorcontrib><creatorcontrib>Ribeiro, J. Alexandre</creatorcontrib><creatorcontrib>Sebastião, Ana M.</creatorcontrib><title>Preferential Release of ATP and Its Extracellular Catabolism as a Source of Adenosine upon High‐ but Not Low‐Frequency Stimulation of Rat Hippocampal Slices</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>: The release of adenosine and ATP evoked by electrical field stimulation in rat hippocampal slices was investigated with the following two patterns of stimulation: (1) a brief, high‐frequency burst stimulation (trains of stimuli at 100 Hz for 50 ms applied every 2 s for 1 min), to mimic a long‐term potentiation (LTP) stimulation paradigm, and (2) a more prolonged (3 min) and low‐frequency (5 Hz) train stimulation, to mimic a long‐term depression (LTD) stimulation paradigm. The release of ATP was greater at a brief, high‐frequency burst stimulation, whereas the release of [3H]adenosine was slightly greater at a more prolonged and low‐frequency stimulation. To investigate the source of extracellular adenosine, the following two pharmacological tools were used; α,β‐methylene ADP (AOPCP), an inhibitor of ecto‐5′‐nucleotidase, to assess the contribution of the catabolism of released adenine nucleotides as a source of extracellular adenosine, and S‐(4‐nitrobenzyl)‐6‐thioinosine (NBTI), an inhibitor of adenosine transporters, to assess the contribution of the release of adenosine, as such, as a source of extracellular adenosine. At low‐frequency stimulation, NBTI inhibited by nearly 50% the evoked outflow of [3H]adenosine, whereas AOPCP inhibited [3H]adenosine outflow only marginally. In contrast, at high‐frequency stimulation, AOPCP inhibited by 30% the evoked release of [3H]adenosine, whereas NBTI produced a 40% inhibition of [3H]adenosine outflow. At both frequencies, the kinetics of evoked [3H]adenosine outflow was affected in different manners by AOPCP and NBTI; NBTI mainly depressed the rate of evoked [3H]adenosine outflow, whereas AOPCP mainly inhibited the later phase of evoked [3H]adenosine accumulation. These results show that there is a simultaneous, but quantitatively different, release of ATP and adenosine from rat hippocampal slices stimulated at frequencies that can induce plasticity phenomena such as LTP (100 Hz) or LTD (5 Hz). The source of extracellular adenosine is also different according to the frequency of stimulation; i.e., at a brief, high‐frequency stimulation there is a greater contribution of released adenine nucleotides for the formation of extracellular adenosine than at a low frequency with a more prolonged stimulation.</description><subject>5'-Nucleotidase - antagonists &amp; inhibitors</subject><subject>Adenosine</subject><subject>Adenosine - metabolism</subject><subject>Adenosine Diphosphate - analogs &amp; derivatives</subject><subject>Adenosine Diphosphate - pharmacology</subject><subject>Adenosine Triphosphate - metabolism</subject><subject>Animals</subject><subject>ATP</subject><subject>Biochemistry and metabolism</subject><subject>Biological and medical sciences</subject><subject>Central nervous system</subject><subject>Ectonucleotidases</subject><subject>Electric Stimulation</subject><subject>Energy Metabolism</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hippocampus</subject><subject>Hippocampus - metabolism</subject><subject>Hippocampus - physiology</subject><subject>In Vitro Techniques</subject><subject>Kinetics</subject><subject>Long-Term Potentiation</subject><subject>Male</subject><subject>Models, Neurological</subject><subject>Neurons - cytology</subject><subject>Neurons - metabolism</subject><subject>Neurons - physiology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Synaptic plasticity</subject><subject>Time Factors</subject><subject>Tritium</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkd9u0zAUxiMEGmXwCEiWQNwl-H8dcTWVjQ1V27SOa8t1TsBVEgc70do7HoFH4Nl4Ehy16y3alXX8_b5zjv1l2TuCC4K5_LgpCJ-TnBNRFqQsZSHnWFCicLF9ls2O2vNshjGlOcOcvsxexbjBmEguyUl2opRkgpaz7M9tgBoCdIMzDbqDBkwE5Gt0dn-LTFehqyGi8-0QjIWmGRsT0MIMZu0bF1tkIjJo5cdg954KOh9dB2jsfYcu3fcff3_9RutxQNd-QEv_kMqLAD9H6OwOrQbXpo6DS2xy35khWfreW9P2aZlV4yzE19mL2jQR3hzO0-zbxfn94jJf3ny5Wpwtc8vZHOdQybVhsmJUMqWoAFkCEEYEJ7iygtASr5nB6bbCqqyJArBVzYFiJZSlgp1mH_Z9--DTfnHQrYvTm00Hfox6rjjHlMn_gkSokoqSJvDTHrTBx5i-WffBtSbsNMF6ClJv9BSWnsLSU5D6MUi9Te63hzHjuoXq6D0kl_T3B91Ea5o6mM66eMQYFYorkrDPe-zBNbB7ygb66_XisWL_AL8nvWk</recordid><startdate>199611</startdate><enddate>199611</enddate><creator>Cunha, Rodrigo A.</creator><creator>Vizi, E. Sylvester</creator><creator>Ribeiro, J. Alexandre</creator><creator>Sebastião, Ana M.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>199611</creationdate><title>Preferential Release of ATP and Its Extracellular Catabolism as a Source of Adenosine upon High‐ but Not Low‐Frequency Stimulation of Rat Hippocampal Slices</title><author>Cunha, Rodrigo A. ; Vizi, E. Sylvester ; Ribeiro, J. Alexandre ; Sebastião, Ana M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4370-ed6ba36d32638825e69ee1315410dc51290b3a069ed089f18eecdf4e20858c253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>5'-Nucleotidase - antagonists &amp; inhibitors</topic><topic>Adenosine</topic><topic>Adenosine - metabolism</topic><topic>Adenosine Diphosphate - analogs &amp; derivatives</topic><topic>Adenosine Diphosphate - pharmacology</topic><topic>Adenosine Triphosphate - metabolism</topic><topic>Animals</topic><topic>ATP</topic><topic>Biochemistry and metabolism</topic><topic>Biological and medical sciences</topic><topic>Central nervous system</topic><topic>Ectonucleotidases</topic><topic>Electric Stimulation</topic><topic>Energy Metabolism</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hippocampus</topic><topic>Hippocampus - metabolism</topic><topic>Hippocampus - physiology</topic><topic>In Vitro Techniques</topic><topic>Kinetics</topic><topic>Long-Term Potentiation</topic><topic>Male</topic><topic>Models, Neurological</topic><topic>Neurons - cytology</topic><topic>Neurons - metabolism</topic><topic>Neurons - physiology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Synaptic plasticity</topic><topic>Time Factors</topic><topic>Tritium</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cunha, Rodrigo A.</creatorcontrib><creatorcontrib>Vizi, E. Sylvester</creatorcontrib><creatorcontrib>Ribeiro, J. Alexandre</creatorcontrib><creatorcontrib>Sebastião, Ana M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cunha, Rodrigo A.</au><au>Vizi, E. Sylvester</au><au>Ribeiro, J. Alexandre</au><au>Sebastião, Ana M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preferential Release of ATP and Its Extracellular Catabolism as a Source of Adenosine upon High‐ but Not Low‐Frequency Stimulation of Rat Hippocampal Slices</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>1996-11</date><risdate>1996</risdate><volume>67</volume><issue>5</issue><spage>2180</spage><epage>2187</epage><pages>2180-2187</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>: The release of adenosine and ATP evoked by electrical field stimulation in rat hippocampal slices was investigated with the following two patterns of stimulation: (1) a brief, high‐frequency burst stimulation (trains of stimuli at 100 Hz for 50 ms applied every 2 s for 1 min), to mimic a long‐term potentiation (LTP) stimulation paradigm, and (2) a more prolonged (3 min) and low‐frequency (5 Hz) train stimulation, to mimic a long‐term depression (LTD) stimulation paradigm. The release of ATP was greater at a brief, high‐frequency burst stimulation, whereas the release of [3H]adenosine was slightly greater at a more prolonged and low‐frequency stimulation. To investigate the source of extracellular adenosine, the following two pharmacological tools were used; α,β‐methylene ADP (AOPCP), an inhibitor of ecto‐5′‐nucleotidase, to assess the contribution of the catabolism of released adenine nucleotides as a source of extracellular adenosine, and S‐(4‐nitrobenzyl)‐6‐thioinosine (NBTI), an inhibitor of adenosine transporters, to assess the contribution of the release of adenosine, as such, as a source of extracellular adenosine. At low‐frequency stimulation, NBTI inhibited by nearly 50% the evoked outflow of [3H]adenosine, whereas AOPCP inhibited [3H]adenosine outflow only marginally. In contrast, at high‐frequency stimulation, AOPCP inhibited by 30% the evoked release of [3H]adenosine, whereas NBTI produced a 40% inhibition of [3H]adenosine outflow. At both frequencies, the kinetics of evoked [3H]adenosine outflow was affected in different manners by AOPCP and NBTI; NBTI mainly depressed the rate of evoked [3H]adenosine outflow, whereas AOPCP mainly inhibited the later phase of evoked [3H]adenosine accumulation. These results show that there is a simultaneous, but quantitatively different, release of ATP and adenosine from rat hippocampal slices stimulated at frequencies that can induce plasticity phenomena such as LTP (100 Hz) or LTD (5 Hz). The source of extracellular adenosine is also different according to the frequency of stimulation; i.e., at a brief, high‐frequency stimulation there is a greater contribution of released adenine nucleotides for the formation of extracellular adenosine than at a low frequency with a more prolonged stimulation.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>8863529</pmid><doi>10.1046/j.1471-4159.1996.67052180.x</doi><tpages>8</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0022-3042
ispartof Journal of neurochemistry, 1996-11, Vol.67 (5), p.2180-2187
issn 0022-3042
1471-4159
language eng
recordid cdi_proquest_miscellaneous_78440236
source MEDLINE; Access via Wiley Online Library
subjects 5'-Nucleotidase - antagonists & inhibitors
Adenosine
Adenosine - metabolism
Adenosine Diphosphate - analogs & derivatives
Adenosine Diphosphate - pharmacology
Adenosine Triphosphate - metabolism
Animals
ATP
Biochemistry and metabolism
Biological and medical sciences
Central nervous system
Ectonucleotidases
Electric Stimulation
Energy Metabolism
Enzyme Inhibitors - pharmacology
Fundamental and applied biological sciences. Psychology
Hippocampus
Hippocampus - metabolism
Hippocampus - physiology
In Vitro Techniques
Kinetics
Long-Term Potentiation
Male
Models, Neurological
Neurons - cytology
Neurons - metabolism
Neurons - physiology
Rats
Rats, Wistar
Synaptic plasticity
Time Factors
Tritium
Vertebrates: nervous system and sense organs
title Preferential Release of ATP and Its Extracellular Catabolism as a Source of Adenosine upon High‐ but Not Low‐Frequency Stimulation of Rat Hippocampal Slices
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T21%3A38%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Preferential%20Release%20of%20ATP%20and%20Its%20Extracellular%20Catabolism%20as%20a%20Source%20of%20Adenosine%20upon%20High%E2%80%90%20but%20Not%20Low%E2%80%90Frequency%20Stimulation%20of%20Rat%20Hippocampal%20Slices&rft.jtitle=Journal%20of%20neurochemistry&rft.au=Cunha,%20Rodrigo%20A.&rft.date=1996-11&rft.volume=67&rft.issue=5&rft.spage=2180&rft.epage=2187&rft.pages=2180-2187&rft.issn=0022-3042&rft.eissn=1471-4159&rft.coden=JONRA9&rft_id=info:doi/10.1046/j.1471-4159.1996.67052180.x&rft_dat=%3Cproquest_cross%3E78440236%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15892592&rft_id=info:pmid/8863529&rfr_iscdi=true