Restoration of antibody binding to blotted meningococcal outer membrane proteins using various detergents

Restoration of IgG antibody binding to heat-denatured meningococcal outer membrane proteins has been studied on immunoblots with a series of 14 detergents. Nitrocellulose strips with the blotted proteins were incubated with the detergents and sera from human volunteers vaccinated with meningococcal...

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Veröffentlicht in:	Journal of immunological methods 1988-10, Vol.113 (1), p.51-59
Hauptverfasser:	Wedege, E., Bryn, K., Frøholm, L.O.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Bacterial Outer Membrane Proteins - isolation & purification Binding Sites, Antibody Cetrimonium Cetrimonium Compounds Detergent Detergents Humans Immunoblotting Immunoblotting - methods Immunoglobulin G - metabolism Lipopolysaccharide Lipopolysaccharides Meningococcal porin Neisseria meningitidis - analysis Organic Chemicals Protein Denaturation Quaternary Ammonium Compounds Renaturation Sarcosine - analogs & derivatives Surface-Active Agents
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