Anti-HIV antibody in saliva: an assessment of the role of the components of saliva, testing methodologies and collection systems

The various components of saliva, namely mixed saliva, parotid saliva, submandibular saliva, crevicular fluid and minor (labial) gland secretions, were collected from 63 known HIV antibody seropositive patients. A commercial test system. Wellcozyme HIV 1+2, and an antibody capture ELISA (GACELISA)....

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Veröffentlicht in:Journal of oral pathology & medicine 1996-03, Vol.25 (3), p.104-107
Hauptverfasser: Lamey, P.-J., Nolan, A., Follett, E. A. C., Coote, I., MacFarlane, T. W., Kennedy, D. H., Connell, A., Parry, J. V.
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container_end_page 107
container_issue 3
container_start_page 104
container_title Journal of oral pathology & medicine
container_volume 25
creator Lamey, P.-J.
Nolan, A.
Follett, E. A. C.
Coote, I.
MacFarlane, T. W.
Kennedy, D. H.
Connell, A.
Parry, J. V.
description The various components of saliva, namely mixed saliva, parotid saliva, submandibular saliva, crevicular fluid and minor (labial) gland secretions, were collected from 63 known HIV antibody seropositive patients. A commercial test system. Wellcozyme HIV 1+2, and an antibody capture ELISA (GACELISA). were compared for sensitivity against all components. Sensitivity of the GACELISA system was 100%. in 123 mixed saliva, 121 parotid saliva and 127 labial fluid samples, and 98% in 99 submandibular samples and 127 crevicular fluid samples. Respective figures for Wellcozyme 1+2 were 92%, 55% 73%, 66% and 63%. Mixed saliva was most easily, conveniently and effectively collected using a plain Salivette. In 241 Salivette samples examined from the 63 patients. GACELISA proved 100% sensitive, and Wellcozyme 95% sensitive. Another form of Salivette impregnated with citric acid was unsuitable for GACELISA and gave a false negative value of 45%. In 197 samples from the gingival margin taken by a dry swab. GACELISA showed a sensitivity of 98% and Wellcozyme 81%. The most sensitive method for demonstrating anti‐HIV antibody in saliva is to collect mixed saliva with the plain Salivette system and assay anti‐HIV antibody levels by GACELISA.
doi_str_mv 10.1111/j.1600-0714.1996.tb00202.x
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In 241 Salivette samples examined from the 63 patients. GACELISA proved 100% sensitive, and Wellcozyme 95% sensitive. Another form of Salivette impregnated with citric acid was unsuitable for GACELISA and gave a false negative value of 45%. In 197 samples from the gingival margin taken by a dry swab. GACELISA showed a sensitivity of 98% and Wellcozyme 81%. 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A. C.</creatorcontrib><creatorcontrib>Coote, I.</creatorcontrib><creatorcontrib>MacFarlane, T. W.</creatorcontrib><creatorcontrib>Kennedy, D. H.</creatorcontrib><creatorcontrib>Connell, A.</creatorcontrib><creatorcontrib>Parry, J. V.</creatorcontrib><title>Anti-HIV antibody in saliva: an assessment of the role of the components of saliva, testing methodologies and collection systems</title><title>Journal of oral pathology &amp; medicine</title><addtitle>J Oral Pathol Med</addtitle><description>The various components of saliva, namely mixed saliva, parotid saliva, submandibular saliva, crevicular fluid and minor (labial) gland secretions, were collected from 63 known HIV antibody seropositive patients. A commercial test system. Wellcozyme HIV 1+2, and an antibody capture ELISA (GACELISA). were compared for sensitivity against all components. 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Immunoglobulinopathies</subject><subject>Immunoglobulin G - analysis</subject><subject>Immunopathology</subject><subject>Lip</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Parotid Gland - secretion</subject><subject>Reagent Kits, Diagnostic</subject><subject>saliva</subject><subject>Saliva - immunology</subject><subject>Salivary Glands, Minor - secretion</subject><subject>Salivette</subject><subject>Sensitivity and Specificity</subject><subject>Specimen Handling - instrumentation</subject><subject>Specimen Handling - methods</subject><subject>Submandibular Gland - secretion</subject><issn>0904-2512</issn><issn>1600-0714</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkE1v1DAQhi0EKkvhJyBFCHEiYZw4Xz0gVRX0QysKEtCjZSeT1osTLxkv7N746ThK2DtzsTXvO--MHsZecUh4qHebhBcAMZRcJLyui8RrgBTSZP-IrY7SY7aCGkSc5jx9yp4RbQB4mQl-wk5qLirI8hX7cz54E19df49U-GjXHiIzRKSs-aXOQi9SREjU4-Aj10X-AaPRWfz3b1y_dUMQaerMY28jj-TNcB_16B9c66y7N0ghrA1-a7HxxoUdB_LY03P2pFOW8MXynrJvHz98vbiK17eX1xfn67gRRS5iVCCgxA6rVGVVh41IeaZqznVb6UJ0Ndclz3XwNAIBsdNtrbs0NDBUW2en7M2cux3dz104UPaGGrRWDeh2JMtKZIFKEYxns7EZHdGIndyOplfjQXKQE365kRNjOTGWE3654Jf7MPxy2bLTPbbH0YV30F8vuqJG2W5UQ2PoaMsgr4qKB9v72fbbWDz8xwHy5vYzBxEC4jnABMj7Y4Aaf8iizMpc3n26lOlNtf4Cd7mss78th7NT</recordid><startdate>199603</startdate><enddate>199603</enddate><creator>Lamey, P.-J.</creator><creator>Nolan, A.</creator><creator>Follett, E. 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V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-HIV antibody in saliva: an assessment of the role of the components of saliva, testing methodologies and collection systems</atitle><jtitle>Journal of oral pathology &amp; medicine</jtitle><addtitle>J Oral Pathol Med</addtitle><date>1996-03</date><risdate>1996</risdate><volume>25</volume><issue>3</issue><spage>104</spage><epage>107</epage><pages>104-107</pages><issn>0904-2512</issn><eissn>1600-0714</eissn><abstract>The various components of saliva, namely mixed saliva, parotid saliva, submandibular saliva, crevicular fluid and minor (labial) gland secretions, were collected from 63 known HIV antibody seropositive patients. A commercial test system. Wellcozyme HIV 1+2, and an antibody capture ELISA (GACELISA). were compared for sensitivity against all components. Sensitivity of the GACELISA system was 100%. in 123 mixed saliva, 121 parotid saliva and 127 labial fluid samples, and 98% in 99 submandibular samples and 127 crevicular fluid samples. Respective figures for Wellcozyme 1+2 were 92%, 55% 73%, 66% and 63%. Mixed saliva was most easily, conveniently and effectively collected using a plain Salivette. In 241 Salivette samples examined from the 63 patients. GACELISA proved 100% sensitive, and Wellcozyme 95% sensitive. Another form of Salivette impregnated with citric acid was unsuitable for GACELISA and gave a false negative value of 45%. In 197 samples from the gingival margin taken by a dry swab. GACELISA showed a sensitivity of 98% and Wellcozyme 81%. The most sensitive method for demonstrating anti‐HIV antibody in saliva is to collect mixed saliva with the plain Salivette system and assay anti‐HIV antibody levels by GACELISA.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>9148035</pmid><doi>10.1111/j.1600-0714.1996.tb00202.x</doi><tpages>4</tpages></addata></record>
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subjects Adult
AIDS/HIV
anti-HIV antibody
antibody capture
Biological and medical sciences
Blotting, Western
Chelating Agents
Citric Acid
Dentistry
Enzyme-Linked Immunosorbent Assay - methods
False Negative Reactions
Female
Gingival Crevicular Fluid - immunology
HIV Antibodies - analysis
HIV Seropositivity - immunology
HIV-1 - immunology
HIV-2 - immunology
Humans
Immunodeficiencies
Immunodeficiencies. Immunoglobulinopathies
Immunoglobulin G - analysis
Immunopathology
Lip
Male
Medical sciences
Middle Aged
Parotid Gland - secretion
Reagent Kits, Diagnostic
saliva
Saliva - immunology
Salivary Glands, Minor - secretion
Salivette
Sensitivity and Specificity
Specimen Handling - instrumentation
Specimen Handling - methods
Submandibular Gland - secretion
title Anti-HIV antibody in saliva: an assessment of the role of the components of saliva, testing methodologies and collection systems
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