Long-term survival and function of isolated bovine pancreatic islets maintained in different culture media
Bovine islets are being evaluated for their potential in transplantation studies. We studied the recovery, morphology, and function of purified bovine islets cultured up to 4 weeks under varying conditions. Approximately 60% of the initial islet mass could be recovered after 4 weeks at 37 degrees C...
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| Veröffentlicht in: | Acta diabetologica 1996-07, Vol.33 (2), p.166-168 |
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| container_end_page | 168 |
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| container_issue | 2 |
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| container_title | Acta diabetologica |
| container_volume | 33 |
| creator | COPPELLI, A ARVIA, C GIANNARELLI, R MARCHETTI, P VIACAVA, P NACCARATO, A. G LORENZETTI, M COSIMI, S CECCHETTI, P NAVALESI, R |
| description | Bovine islets are being evaluated for their potential in transplantation studies. We studied the recovery, morphology, and function of purified bovine islets cultured up to 4 weeks under varying conditions. Approximately 60% of the initial islet mass could be recovered after 4 weeks at 37 degrees C in CMRL 1066 or M 199 culture medium, and the cultured islets were well preserved histologically and viable both in vitro and in vivo. On the other hand, culture with RPMI 1640 caused disaggregation of the islets within a few days, with altered in vitro viability. Thus, culturing purified bovine islets with appropriate media is a suitable procedure to maintain islet mass, morphology, and function in the long term. |
| doi_str_mv | 10.1007/BF00569429 |
| format | Article |
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Thus, culturing purified bovine islets with appropriate media is a suitable procedure to maintain islet mass, morphology, and function in the long term.</description><identifier>ISSN: 0940-5429</identifier><identifier>EISSN: 1432-5233</identifier><identifier>DOI: 10.1007/BF00569429</identifier><identifier>PMID: 8870821</identifier><language>eng</language><publisher>Berlin: Springer International</publisher><subject>Animals ; Biological and medical sciences ; Cattle ; Cell cultures. Hybridization. Fusion ; Culture Media - pharmacology ; Fundamental and applied biological sciences. 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Psychology</subject><subject>General aspects</subject><subject>Immunohistochemistry</subject><subject>Insulin - metabolism</subject><subject>Insulin Secretion</subject><subject>Islets of Langerhans - drug effects</subject><subject>Islets of Langerhans - physiology</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Organ Transplantation</subject><issn>0940-5429</issn><issn>1432-5233</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtLBDEQhIMouj4u3oUcxIMw2knmeVTxBQte9Dz0Jh2JzGTWJLPgvzfiooem6a6PgirGTgVcCYDm-vYBoKq7UnY7bCFKJYtKKrXLFtCVUFT5f8AOY_wAELJR7T7bb9sGWikW7GM5-fciURh5nMPGbXDg6A23s9fJTZ5Plrs4DZjI8NW0cZ74Gr0OhMnpLA2UIh_R-ZQnM85z46ylQD5xPQ9pDsRHMg6P2Z7FIdLJdh-xt4f717unYvny-Hx3syy0EiIVJkeqUK2EobqrrCEwqNEiIBqUdSNAidJIrbVBtBKwyjcKMqCxRtWqI3bx67sO0-dMMfWji5qGAT1Nc-ybtlSN7FQGL39BHaYYA9l-HdyI4asX0P8U2_8Xm-Gzreu8ynH-0G2TWT_f6hg1Djbkklz8w5Royhpq9Q0dxYId</recordid><startdate>19960701</startdate><enddate>19960701</enddate><creator>COPPELLI, A</creator><creator>ARVIA, C</creator><creator>GIANNARELLI, R</creator><creator>MARCHETTI, P</creator><creator>VIACAVA, P</creator><creator>NACCARATO, A. 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| fulltext | fulltext |
| identifier | ISSN: 0940-5429 |
| ispartof | Acta diabetologica, 1996-07, Vol.33 (2), p.166-168 |
| issn | 0940-5429 1432-5233 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animals Biological and medical sciences Cattle Cell cultures. Hybridization. Fusion Culture Media - pharmacology Fundamental and applied biological sciences. Psychology General aspects Immunohistochemistry Insulin - metabolism Insulin Secretion Islets of Langerhans - drug effects Islets of Langerhans - physiology Mice Molecular and cellular biology Organ Transplantation |
| title | Long-term survival and function of isolated bovine pancreatic islets maintained in different culture media |
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