Detection of Autoantibodies to the Pancreatic Islet Heat Shock Protein 60 in Insulin-dependent Diabetes Mellitus
Autoantibodies against heat shock protein (hsp) 60 have been reported to be detected in sera of non-obese diabetic mice, in an experimental model of IDDM. However, there are only a few studies which have examined IDDM patients for antibodies against mammalian hsp60. We produced murine hsp60 derived...
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| Veröffentlicht in: | Journal of autoimmunity 1996-08, Vol.9 (4), p.517-524 |
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| creator | Ozawa, Yukako Kasuga, Akira Nomaguchi, Hiroko Maruyama, Taro Kasatani, Tomohiro Shimada, Akira Takei, Izumi Miyazaki, Jun-ichi Saruta, Takao |
| description | Autoantibodies against heat shock protein (hsp) 60 have been reported to be detected in sera of non-obese diabetic mice, in an experimental model of IDDM. However, there are only a few studies which have examined IDDM patients for antibodies against mammalian hsp60. We produced murine hsp60 derived from pancreatic beta cells which has high homology to human hsp60 and examined antibodies against the hsp60 in IDDM patients using an enzyme-linked immunosorbent assay. We extended the analysis to patients with other immune-mediated diseases and non-insulin-dependent diabetes mellitus (NIDDM). Positive sera for hsp60 antibody were more frequently detected in 13 out of 84 IDDM (15.5%) and 5 out of 25 rheumatoid arthritis patients (20%), when compared to healthy subjects (1/85; 1.2%,
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| doi_str_mv | 10.1006/jaut.1996.0069 |
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P<0.001 and
P<0.01, respectively). The levels of hsp60 antibodies of IDDM (0.218±0.227) and rheumatoid arthritis patients (0.259±0.191) were significantly higher than those of healthy subjects (0.076±0.131,
P<0.001,
P<0.01, respectively). Patients with slowly progressive IDDM (
n=26), autoimmune thyroid disease (
n=42), or NIDDM (
n=40) had levels of hsp60 antibodies similar to those in healthy subjects. We found no relationship between the levels of hsp60 antibodies and islet cell antibodies (ICA) or antibodies to glutamic acid decarboxylase (GAD65) in IDDM patients. In conclusion, hsp60 antibodies were detected in Japanese IDDM as well as in rheumatoid arthritis patients. Although the positivity was low, the detection of hsp60 antibodies may be helpful for diagnosis of IDDM especially in GAD65 Ab- or ICA-negative Japanese patients.</description><identifier>ISSN: 0896-8411</identifier><identifier>EISSN: 1095-9157</identifier><identifier>DOI: 10.1006/jaut.1996.0069</identifier><identifier>PMID: 8864827</identifier><language>eng</language><publisher>London: Elsevier Ltd</publisher><subject>Autoantibodies - blood ; autoantibody ; Biological and medical sciences ; Chaperonin 60 - immunology ; Diabetes Mellitus, Type 1 - blood ; Diabetes Mellitus, Type 1 - immunology ; Diabetes. Impaired glucose tolerance ; ELISA ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; Etiopathogenesis. Screening. Investigations. Target tissue resistance ; Female ; Fluorescent Antibody Technique, Indirect ; heat shock protein 60 ; Humans ; insulin-dependent diabetes mellitus ; Islets of Langerhans - chemistry ; Male ; Medical sciences ; Middle Aged ; rheumatoid arthritis</subject><ispartof>Journal of autoimmunity, 1996-08, Vol.9 (4), p.517-524</ispartof><rights>1996 Academic Press</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-22da130480851dc49e60368712d4db67628883e447f82f1ca643193596c50b8b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/jaut.1996.0069$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3184079$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8864827$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ozawa, Yukako</creatorcontrib><creatorcontrib>Kasuga, Akira</creatorcontrib><creatorcontrib>Nomaguchi, Hiroko</creatorcontrib><creatorcontrib>Maruyama, Taro</creatorcontrib><creatorcontrib>Kasatani, Tomohiro</creatorcontrib><creatorcontrib>Shimada, Akira</creatorcontrib><creatorcontrib>Takei, Izumi</creatorcontrib><creatorcontrib>Miyazaki, Jun-ichi</creatorcontrib><creatorcontrib>Saruta, Takao</creatorcontrib><title>Detection of Autoantibodies to the Pancreatic Islet Heat Shock Protein 60 in Insulin-dependent Diabetes Mellitus</title><title>Journal of autoimmunity</title><addtitle>J Autoimmun</addtitle><description>Autoantibodies against heat shock protein (hsp) 60 have been reported to be detected in sera of non-obese diabetic mice, in an experimental model of IDDM. However, there are only a few studies which have examined IDDM patients for antibodies against mammalian hsp60. We produced murine hsp60 derived from pancreatic beta cells which has high homology to human hsp60 and examined antibodies against the hsp60 in IDDM patients using an enzyme-linked immunosorbent assay. We extended the analysis to patients with other immune-mediated diseases and non-insulin-dependent diabetes mellitus (NIDDM). Positive sera for hsp60 antibody were more frequently detected in 13 out of 84 IDDM (15.5%) and 5 out of 25 rheumatoid arthritis patients (20%), when compared to healthy subjects (1/85; 1.2%,
P<0.001 and
P<0.01, respectively). The levels of hsp60 antibodies of IDDM (0.218±0.227) and rheumatoid arthritis patients (0.259±0.191) were significantly higher than those of healthy subjects (0.076±0.131,
P<0.001,
P<0.01, respectively). Patients with slowly progressive IDDM (
n=26), autoimmune thyroid disease (
n=42), or NIDDM (
n=40) had levels of hsp60 antibodies similar to those in healthy subjects. We found no relationship between the levels of hsp60 antibodies and islet cell antibodies (ICA) or antibodies to glutamic acid decarboxylase (GAD65) in IDDM patients. In conclusion, hsp60 antibodies were detected in Japanese IDDM as well as in rheumatoid arthritis patients. Although the positivity was low, the detection of hsp60 antibodies may be helpful for diagnosis of IDDM especially in GAD65 Ab- or ICA-negative Japanese patients.</description><subject>Autoantibodies - blood</subject><subject>autoantibody</subject><subject>Biological and medical sciences</subject><subject>Chaperonin 60 - immunology</subject><subject>Diabetes Mellitus, Type 1 - blood</subject><subject>Diabetes Mellitus, Type 1 - immunology</subject><subject>Diabetes. Impaired glucose tolerance</subject><subject>ELISA</subject><subject>Endocrine pancreas. Apud cells (diseases)</subject><subject>Endocrinopathies</subject><subject>Etiopathogenesis. Screening. Investigations. Target tissue resistance</subject><subject>Female</subject><subject>Fluorescent Antibody Technique, Indirect</subject><subject>heat shock protein 60</subject><subject>Humans</subject><subject>insulin-dependent diabetes mellitus</subject><subject>Islets of Langerhans - chemistry</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>rheumatoid arthritis</subject><issn>0896-8411</issn><issn>1095-9157</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9rFDEUx4Moda1evQk5iLdZk8mPSY6lrXahYkE9h0zyhqbOJmuSEfzvzbBLb6WXFx7v8755fL8IvadkSwmRnx_sUrdUa7ltnX6BNpRo0WkqhpdoQ5SWneKUvkZvSnkghFIhxBk6U0py1Q8bdLiCCq6GFHGa8MVSk401jMkHKLgmXO8B39noMtgaHN6VGSq-aQ3-cZ_cb3yXU4UQsSS41V0syxxi5-EA0UOs-CrYsf1Q8DeY51CX8ha9muxc4N3pPUe_vlz_vLzpbr9_3V1e3HaOS1G7vveWMsIVUYJ6xzVIwqQaaO-5H-Uge6UUA86HSfUTdVZyRjUTWjpBRjWyc_TpqHvI6c8CpZp9KK4dYSOkpZhBcSYZU8-CVKieDVo0cHsEXU6lZJjMIYe9zf8MJWbNwqxZmDULs2bRFj6clJdxD_4RP5nf5h9Pc1ucnafcfA7lEWNUcTKsMuqIQbPrb4BsigsQHfiQW3TGp_DUBf8B6BqkGA</recordid><startdate>19960801</startdate><enddate>19960801</enddate><creator>Ozawa, Yukako</creator><creator>Kasuga, Akira</creator><creator>Nomaguchi, Hiroko</creator><creator>Maruyama, Taro</creator><creator>Kasatani, Tomohiro</creator><creator>Shimada, Akira</creator><creator>Takei, Izumi</creator><creator>Miyazaki, Jun-ichi</creator><creator>Saruta, Takao</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19960801</creationdate><title>Detection of Autoantibodies to the Pancreatic Islet Heat Shock Protein 60 in Insulin-dependent Diabetes Mellitus</title><author>Ozawa, Yukako ; Kasuga, Akira ; Nomaguchi, Hiroko ; Maruyama, Taro ; Kasatani, Tomohiro ; Shimada, Akira ; Takei, Izumi ; Miyazaki, Jun-ichi ; Saruta, Takao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-22da130480851dc49e60368712d4db67628883e447f82f1ca643193596c50b8b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Autoantibodies - blood</topic><topic>autoantibody</topic><topic>Biological and medical sciences</topic><topic>Chaperonin 60 - immunology</topic><topic>Diabetes Mellitus, Type 1 - blood</topic><topic>Diabetes Mellitus, Type 1 - immunology</topic><topic>Diabetes. Impaired glucose tolerance</topic><topic>ELISA</topic><topic>Endocrine pancreas. Apud cells (diseases)</topic><topic>Endocrinopathies</topic><topic>Etiopathogenesis. Screening. Investigations. Target tissue resistance</topic><topic>Female</topic><topic>Fluorescent Antibody Technique, Indirect</topic><topic>heat shock protein 60</topic><topic>Humans</topic><topic>insulin-dependent diabetes mellitus</topic><topic>Islets of Langerhans - chemistry</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>rheumatoid arthritis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ozawa, Yukako</creatorcontrib><creatorcontrib>Kasuga, Akira</creatorcontrib><creatorcontrib>Nomaguchi, Hiroko</creatorcontrib><creatorcontrib>Maruyama, Taro</creatorcontrib><creatorcontrib>Kasatani, Tomohiro</creatorcontrib><creatorcontrib>Shimada, Akira</creatorcontrib><creatorcontrib>Takei, Izumi</creatorcontrib><creatorcontrib>Miyazaki, Jun-ichi</creatorcontrib><creatorcontrib>Saruta, Takao</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of autoimmunity</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ozawa, Yukako</au><au>Kasuga, Akira</au><au>Nomaguchi, Hiroko</au><au>Maruyama, Taro</au><au>Kasatani, Tomohiro</au><au>Shimada, Akira</au><au>Takei, Izumi</au><au>Miyazaki, Jun-ichi</au><au>Saruta, Takao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Autoantibodies to the Pancreatic Islet Heat Shock Protein 60 in Insulin-dependent Diabetes Mellitus</atitle><jtitle>Journal of autoimmunity</jtitle><addtitle>J Autoimmun</addtitle><date>1996-08-01</date><risdate>1996</risdate><volume>9</volume><issue>4</issue><spage>517</spage><epage>524</epage><pages>517-524</pages><issn>0896-8411</issn><eissn>1095-9157</eissn><abstract>Autoantibodies against heat shock protein (hsp) 60 have been reported to be detected in sera of non-obese diabetic mice, in an experimental model of IDDM. However, there are only a few studies which have examined IDDM patients for antibodies against mammalian hsp60. We produced murine hsp60 derived from pancreatic beta cells which has high homology to human hsp60 and examined antibodies against the hsp60 in IDDM patients using an enzyme-linked immunosorbent assay. We extended the analysis to patients with other immune-mediated diseases and non-insulin-dependent diabetes mellitus (NIDDM). Positive sera for hsp60 antibody were more frequently detected in 13 out of 84 IDDM (15.5%) and 5 out of 25 rheumatoid arthritis patients (20%), when compared to healthy subjects (1/85; 1.2%,
P<0.001 and
P<0.01, respectively). The levels of hsp60 antibodies of IDDM (0.218±0.227) and rheumatoid arthritis patients (0.259±0.191) were significantly higher than those of healthy subjects (0.076±0.131,
P<0.001,
P<0.01, respectively). Patients with slowly progressive IDDM (
n=26), autoimmune thyroid disease (
n=42), or NIDDM (
n=40) had levels of hsp60 antibodies similar to those in healthy subjects. We found no relationship between the levels of hsp60 antibodies and islet cell antibodies (ICA) or antibodies to glutamic acid decarboxylase (GAD65) in IDDM patients. In conclusion, hsp60 antibodies were detected in Japanese IDDM as well as in rheumatoid arthritis patients. Although the positivity was low, the detection of hsp60 antibodies may be helpful for diagnosis of IDDM especially in GAD65 Ab- or ICA-negative Japanese patients.</abstract><cop>London</cop><pub>Elsevier Ltd</pub><pmid>8864827</pmid><doi>10.1006/jaut.1996.0069</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of autoimmunity, 1996-08, Vol.9 (4), p.517-524 |
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| subjects | Autoantibodies - blood autoantibody Biological and medical sciences Chaperonin 60 - immunology Diabetes Mellitus, Type 1 - blood Diabetes Mellitus, Type 1 - immunology Diabetes. Impaired glucose tolerance ELISA Endocrine pancreas. Apud cells (diseases) Endocrinopathies Etiopathogenesis. Screening. Investigations. Target tissue resistance Female Fluorescent Antibody Technique, Indirect heat shock protein 60 Humans insulin-dependent diabetes mellitus Islets of Langerhans - chemistry Male Medical sciences Middle Aged rheumatoid arthritis |
| title | Detection of Autoantibodies to the Pancreatic Islet Heat Shock Protein 60 in Insulin-dependent Diabetes Mellitus |
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