Correlation between clonotypic T-cell receptor beta chain variable region (TCR-Vβ) gene expression and aberrant T-cell antigen expression in cutaneous T-cell lymphoma
Immunohistochemical studies can augment the clinicopathologic diagnosis of cutaneous T‐cell lymphoma (CTCL). Our goal was to determine whether a panel of 11 T‐cell receptor (TCR) beta chain variable region (Vβ) monoclonal antibodies (moAbs) could consistently identify clonal T‐cell populations withi...
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| Veröffentlicht in: | Journal of cutaneous pathology 1996-08, Vol.23 (4), p.306-311 |
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| container_title | Journal of cutaneous pathology |
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| creator | Finn, Daniel T. Jaworsky, Christine Chooback, Lilian Jensen, Pamela J. Lessin, Stuart R. |
| description | Immunohistochemical studies can augment the clinicopathologic diagnosis of cutaneous T‐cell lymphoma (CTCL). Our goal was to determine whether a panel of 11 T‐cell receptor (TCR) beta chain variable region (Vβ) monoclonal antibodies (moAbs) could consistently identify clonal T‐cell populations within CTCL skin infiltrates, and whether these cells exhibited aberrant T‐cell antigen expression. Biopsies from 24 CTCL and 3 parapsoriasis patients were analyzed. Of the 27 patients, 4 (15%) demonstrated T‐cell clonality by restricted TCR‐Vp moAb staining. The Vp+ restricted cells expressed aberrant antigen profiles. Overall, aberrant antigen profiles were detected in 18/24 (75%) CTCL patients. Vβ18 moAb crossreacted with a 85 kD protein produced by basal and suprabasal keratinocytes. We conclude: 1) Restricted TCR‐Vp expression correlated with aberrant T‐cell antigen profiles; 2) In the absence of a complete panel of TCR‐Vp moAbs, localization of aberrant T‐cell antigen expression can be useful in identifying malignant T‐cells within CTCL skin infiltrates; 3) The detection sensitivity and specificity of the currently available TCR‐Vβ moAbs may limit their utility to consistently detect clonal T‐cell populations in CTCL skin biopsies; 4) A 85 kD protein present on basal and suprabasal keratinocytes is recognized by Vβ18 moAb and may be related to immune function (s) of the epidermis. |
| doi_str_mv | 10.1111/j.1600-0560.1996.tb01302.x |
| format | Article |
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Our goal was to determine whether a panel of 11 T‐cell receptor (TCR) beta chain variable region (Vβ) monoclonal antibodies (moAbs) could consistently identify clonal T‐cell populations within CTCL skin infiltrates, and whether these cells exhibited aberrant T‐cell antigen expression. Biopsies from 24 CTCL and 3 parapsoriasis patients were analyzed. Of the 27 patients, 4 (15%) demonstrated T‐cell clonality by restricted TCR‐Vp moAb staining. The Vp+ restricted cells expressed aberrant antigen profiles. Overall, aberrant antigen profiles were detected in 18/24 (75%) CTCL patients. Vβ18 moAb crossreacted with a 85 kD protein produced by basal and suprabasal keratinocytes. We conclude: 1) Restricted TCR‐Vp expression correlated with aberrant T‐cell antigen profiles; 2) In the absence of a complete panel of TCR‐Vp moAbs, localization of aberrant T‐cell antigen expression can be useful in identifying malignant T‐cells within CTCL skin infiltrates; 3) The detection sensitivity and specificity of the currently available TCR‐Vβ moAbs may limit their utility to consistently detect clonal T‐cell populations in CTCL skin biopsies; 4) A 85 kD protein present on basal and suprabasal keratinocytes is recognized by Vβ18 moAb and may be related to immune function (s) of the epidermis.</description><identifier>ISSN: 0303-6987</identifier><identifier>EISSN: 1600-0560</identifier><identifier>DOI: 10.1111/j.1600-0560.1996.tb01302.x</identifier><identifier>PMID: 8864916</identifier><identifier>CODEN: JCUPBN</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Antigens, Differentiation, T-Lymphocyte - biosynthesis ; Biological and medical sciences ; Biomarkers, Tumor - biosynthesis ; Blotting, Western ; Clone Cells ; Gene Expression - genetics ; Hematologic and hematopoietic diseases ; Humans ; Immunohistochemistry ; Leukemias. Malignant lymphomas. Malignant reticulosis. 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Our goal was to determine whether a panel of 11 T‐cell receptor (TCR) beta chain variable region (Vβ) monoclonal antibodies (moAbs) could consistently identify clonal T‐cell populations within CTCL skin infiltrates, and whether these cells exhibited aberrant T‐cell antigen expression. Biopsies from 24 CTCL and 3 parapsoriasis patients were analyzed. Of the 27 patients, 4 (15%) demonstrated T‐cell clonality by restricted TCR‐Vp moAb staining. The Vp+ restricted cells expressed aberrant antigen profiles. Overall, aberrant antigen profiles were detected in 18/24 (75%) CTCL patients. Vβ18 moAb crossreacted with a 85 kD protein produced by basal and suprabasal keratinocytes. We conclude: 1) Restricted TCR‐Vp expression correlated with aberrant T‐cell antigen profiles; 2) In the absence of a complete panel of TCR‐Vp moAbs, localization of aberrant T‐cell antigen expression can be useful in identifying malignant T‐cells within CTCL skin infiltrates; 3) The detection sensitivity and specificity of the currently available TCR‐Vβ moAbs may limit their utility to consistently detect clonal T‐cell populations in CTCL skin biopsies; 4) A 85 kD protein present on basal and suprabasal keratinocytes is recognized by Vβ18 moAb and may be related to immune function (s) of the epidermis.</description><subject>Antigens, Differentiation, T-Lymphocyte - biosynthesis</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - biosynthesis</subject><subject>Blotting, Western</subject><subject>Clone Cells</subject><subject>Gene Expression - genetics</subject><subject>Hematologic and hematopoietic diseases</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</subject><subject>Lymphoma, T-Cell - metabolism</subject><subject>Medical sciences</subject><subject>Receptors, Antigen, T-Cell, alpha-beta - biosynthesis</subject><subject>Receptors, Antigen, T-Cell, alpha-beta - genetics</subject><subject>Skin Neoplasms - metabolism</subject><issn>0303-6987</issn><issn>1600-0560</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkc9u1DAQxi0EKsvCIyBFCCE4ZLHjxEl6QEIRLEhVgbKFozWxJ62X_MPO0t0n4s6D8Ew4JF1xZS62_P3mm5E_Qp4wumK-Xm5XTFAa0kT4hzwXq6GkjNNotb9DFkfpLllQTnko8iy9Tx44t6WUiUwkJ-Qky0ScM7EgP4vOWqxhMF0blDjcILaBqru2Gw69UcEmVFjXgUWF_dDZEYFAXYNpgx9gDZQ1evFq7H6-KS7CL79_vQiusMUA971F50YFWh1AidZCO9w6-qvx3L-Y91S7AVrsdu4Wqw9Nf9018JDcq6B2-Gg-l-Ty7ZtN8S48-7B-X7w-C1XMhQiBVxXVqBWkjPMYqlgLrkWkYwVKa011mpeUJnHFOEQRpUCjvBJRlWPKOcZ8SZ5Nvr3tvu_QDbIxbtxkWkumWcz_1pKcTqCynXMWK9lb04A9SEblmJLcyjEKOUYhx5TknJLc--bH85Rd2aA-ts6xeP3prINTUFf-45RxR4yzNOFx6rFXE3Zjajz8xwKyuPzI6TgnnAyMG3B_NAD7TYqUp4n8er6W68_x-Sb_xOQF_wMt1sIf</recordid><startdate>199608</startdate><enddate>199608</enddate><creator>Finn, Daniel T.</creator><creator>Jaworsky, Christine</creator><creator>Chooback, Lilian</creator><creator>Jensen, Pamela J.</creator><creator>Lessin, Stuart R.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199608</creationdate><title>Correlation between clonotypic T-cell receptor beta chain variable region (TCR-Vβ) gene expression and aberrant T-cell antigen expression in cutaneous T-cell lymphoma</title><author>Finn, Daniel T. ; Jaworsky, Christine ; Chooback, Lilian ; Jensen, Pamela J. ; Lessin, Stuart R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4366-a3ff0dedca71334af4d63d62d4cacddd0d79b0054f13a2200a029f62f9e733e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Antigens, Differentiation, T-Lymphocyte - biosynthesis</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - biosynthesis</topic><topic>Blotting, Western</topic><topic>Clone Cells</topic><topic>Gene Expression - genetics</topic><topic>Hematologic and hematopoietic diseases</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis</topic><topic>Lymphoma, T-Cell - metabolism</topic><topic>Medical sciences</topic><topic>Receptors, Antigen, T-Cell, alpha-beta - biosynthesis</topic><topic>Receptors, Antigen, T-Cell, alpha-beta - genetics</topic><topic>Skin Neoplasms - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Finn, Daniel T.</creatorcontrib><creatorcontrib>Jaworsky, Christine</creatorcontrib><creatorcontrib>Chooback, Lilian</creatorcontrib><creatorcontrib>Jensen, Pamela J.</creatorcontrib><creatorcontrib>Lessin, Stuart R.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cutaneous pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Finn, Daniel T.</au><au>Jaworsky, Christine</au><au>Chooback, Lilian</au><au>Jensen, Pamela J.</au><au>Lessin, Stuart R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Correlation between clonotypic T-cell receptor beta chain variable region (TCR-Vβ) gene expression and aberrant T-cell antigen expression in cutaneous T-cell lymphoma</atitle><jtitle>Journal of cutaneous pathology</jtitle><addtitle>J Cutan Pathol</addtitle><date>1996-08</date><risdate>1996</risdate><volume>23</volume><issue>4</issue><spage>306</spage><epage>311</epage><pages>306-311</pages><issn>0303-6987</issn><eissn>1600-0560</eissn><coden>JCUPBN</coden><abstract>Immunohistochemical studies can augment the clinicopathologic diagnosis of cutaneous T‐cell lymphoma (CTCL). Our goal was to determine whether a panel of 11 T‐cell receptor (TCR) beta chain variable region (Vβ) monoclonal antibodies (moAbs) could consistently identify clonal T‐cell populations within CTCL skin infiltrates, and whether these cells exhibited aberrant T‐cell antigen expression. Biopsies from 24 CTCL and 3 parapsoriasis patients were analyzed. Of the 27 patients, 4 (15%) demonstrated T‐cell clonality by restricted TCR‐Vp moAb staining. The Vp+ restricted cells expressed aberrant antigen profiles. Overall, aberrant antigen profiles were detected in 18/24 (75%) CTCL patients. Vβ18 moAb crossreacted with a 85 kD protein produced by basal and suprabasal keratinocytes. We conclude: 1) Restricted TCR‐Vp expression correlated with aberrant T‐cell antigen profiles; 2) In the absence of a complete panel of TCR‐Vp moAbs, localization of aberrant T‐cell antigen expression can be useful in identifying malignant T‐cells within CTCL skin infiltrates; 3) The detection sensitivity and specificity of the currently available TCR‐Vβ moAbs may limit their utility to consistently detect clonal T‐cell populations in CTCL skin biopsies; 4) A 85 kD protein present on basal and suprabasal keratinocytes is recognized by Vβ18 moAb and may be related to immune function (s) of the epidermis.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>8864916</pmid><doi>10.1111/j.1600-0560.1996.tb01302.x</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of cutaneous pathology, 1996-08, Vol.23 (4), p.306-311 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Antigens, Differentiation, T-Lymphocyte - biosynthesis Biological and medical sciences Biomarkers, Tumor - biosynthesis Blotting, Western Clone Cells Gene Expression - genetics Hematologic and hematopoietic diseases Humans Immunohistochemistry Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Lymphoma, T-Cell - metabolism Medical sciences Receptors, Antigen, T-Cell, alpha-beta - biosynthesis Receptors, Antigen, T-Cell, alpha-beta - genetics Skin Neoplasms - metabolism |
| title | Correlation between clonotypic T-cell receptor beta chain variable region (TCR-Vβ) gene expression and aberrant T-cell antigen expression in cutaneous T-cell lymphoma |
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