Application of Primary Hepatocytes from p53-Knockout Mice for Studies of Expression of Cyp3a
CYP3A rapidly disappears in primary hepatocytes, although the primary cells are suitable for studies of the regulation of CYP3A genes. In the present study, we found that Cyp3a mRNA could be expressed in the primary hepatocytes from p53-knockout mice for at least 2 weeks when the cells were cultured...
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| Veröffentlicht in: | Journal of biochemistry (Tokyo) 1996-07, Vol.120 (1), p.42-48 |
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| container_title | Journal of biochemistry (Tokyo) |
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| creator | Shimoji, Miyuki Itoh, Susumu Toide, Kenji Nakayama, Kazuo Aizawa, Shinichi Kamataki, Tetsuya |
| description | CYP3A rapidly disappears in primary hepatocytes, although the primary cells are suitable for studies of the regulation of CYP3A genes. In the present study, we found that Cyp3a mRNA could be expressed in the primary hepatocytes from p53-knockout mice for at least 2 weeks when the cells were cultured in the presence of dexamethasone. Propoxycoumarin O-depropylase activity, which is known to be mainly catalyzed by CYP3A, was maintained at a level of 50% of the initial activity even after 5 days of culture, and the activity correlated with the expression level of Cyp3a mRNA in the primary hepatocytes from p53-knockout mice. The cells remained morphologically intact during 4 weeks. These results suggest that hepatocytes from p53-knockout mice are a useful tool for studies of the expression of Cyp3a. |
| doi_str_mv | 10.1093/oxfordjournals.jbchem.a021391 |
| format | Article |
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In the present study, we found that Cyp3a mRNA could be expressed in the primary hepatocytes from p53-knockout mice for at least 2 weeks when the cells were cultured in the presence of dexamethasone. Propoxycoumarin O-depropylase activity, which is known to be mainly catalyzed by CYP3A, was maintained at a level of 50% of the initial activity even after 5 days of culture, and the activity correlated with the expression level of Cyp3a mRNA in the primary hepatocytes from p53-knockout mice. The cells remained morphologically intact during 4 weeks. These results suggest that hepatocytes from p53-knockout mice are a useful tool for studies of the expression of Cyp3a.</description><identifier>ISSN: 0021-924X</identifier><identifier>DOI: 10.1093/oxfordjournals.jbchem.a021391</identifier><identifier>PMID: 8864842</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>7-Alkoxycoumarin O-Dealkylase - metabolism ; Animals ; Aryl Hydrocarbon Hydroxylases ; Cell Division ; Cells, Cultured ; Cyp3a ; Cytochrome P-450 CYP3A ; Cytochrome P-450 Enzyme System - genetics ; dexamethasone ; Dexamethasone - pharmacology ; Epidermal Growth Factor - pharmacology ; Gene Expression Regulation, Enzymologic - physiology ; Hepatocyte Growth Factor - pharmacology ; hepatocytes ; Insulin - pharmacology ; Liver - cytology ; Liver - drug effects ; Liver - enzymology ; Male ; Mice ; Mice, Knockout ; Oxidoreductases, N-Demethylating - genetics ; p53 ; primary culture ; RNA, Messenger - analysis ; Tumor Suppressor Protein p53 - physiology</subject><ispartof>Journal of biochemistry (Tokyo), 1996-07, Vol.120 (1), p.42-48</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8864842$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shimoji, Miyuki</creatorcontrib><creatorcontrib>Itoh, Susumu</creatorcontrib><creatorcontrib>Toide, Kenji</creatorcontrib><creatorcontrib>Nakayama, Kazuo</creatorcontrib><creatorcontrib>Aizawa, Shinichi</creatorcontrib><creatorcontrib>Kamataki, Tetsuya</creatorcontrib><title>Application of Primary Hepatocytes from p53-Knockout Mice for Studies of Expression of Cyp3a</title><title>Journal of biochemistry (Tokyo)</title><addtitle>J Biochem</addtitle><description>CYP3A rapidly disappears in primary hepatocytes, although the primary cells are suitable for studies of the regulation of CYP3A genes. In the present study, we found that Cyp3a mRNA could be expressed in the primary hepatocytes from p53-knockout mice for at least 2 weeks when the cells were cultured in the presence of dexamethasone. Propoxycoumarin O-depropylase activity, which is known to be mainly catalyzed by CYP3A, was maintained at a level of 50% of the initial activity even after 5 days of culture, and the activity correlated with the expression level of Cyp3a mRNA in the primary hepatocytes from p53-knockout mice. The cells remained morphologically intact during 4 weeks. These results suggest that hepatocytes from p53-knockout mice are a useful tool for studies of the expression of Cyp3a.</description><subject>7-Alkoxycoumarin O-Dealkylase - metabolism</subject><subject>Animals</subject><subject>Aryl Hydrocarbon Hydroxylases</subject><subject>Cell Division</subject><subject>Cells, Cultured</subject><subject>Cyp3a</subject><subject>Cytochrome P-450 CYP3A</subject><subject>Cytochrome P-450 Enzyme System - genetics</subject><subject>dexamethasone</subject><subject>Dexamethasone - pharmacology</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>Gene Expression Regulation, Enzymologic - physiology</subject><subject>Hepatocyte Growth Factor - pharmacology</subject><subject>hepatocytes</subject><subject>Insulin - pharmacology</subject><subject>Liver - cytology</subject><subject>Liver - drug effects</subject><subject>Liver - enzymology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Oxidoreductases, N-Demethylating - genetics</subject><subject>p53</subject><subject>primary culture</subject><subject>RNA, Messenger - analysis</subject><subject>Tumor Suppressor Protein p53 - physiology</subject><issn>0021-924X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9PwkAQxfegQUQ_gkkveit2u9t29-CBEBEjKsE_IcRks2ynsUDZutsm8O1d0obEk6eZyXtvZvJD6BoHfRxwcqt3mTbpStdmKze2v1qqbyj6Mggx4fgEdQPX-Tyk8zN0bu3qMIaEdFCHsZgyGnbR16AsN7mSVa63ns68qckLafbeGEpZabWvwHqZ0YVXRsR_2mq11nXlPecKPHfae6vqNHcWl7zflQasbfcM9yWRF-g0c3_BZVt76GN0_z4c-5PXh8fhYOIrGoSVzziwUCWpkirBNIo5yWIWUYy5VERCBClmaaBixuOUU6eFGFSsAEsiU8Uy0kM3zd7S6J8abCWK3CrYbOQWdG1FwigJcZz8a8RxQBiPuDPeNUZltLUGMlE2YAQOxAG9-IteNOhFi97lr9pD9bKA9JhuuTvdb_TcVrA7ytKshXszicR4vhAvn5MZXcymYkR-AXN3meY</recordid><startdate>19960701</startdate><enddate>19960701</enddate><creator>Shimoji, Miyuki</creator><creator>Itoh, Susumu</creator><creator>Toide, Kenji</creator><creator>Nakayama, Kazuo</creator><creator>Aizawa, Shinichi</creator><creator>Kamataki, Tetsuya</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19960701</creationdate><title>Application of Primary Hepatocytes from p53-Knockout Mice for Studies of Expression of Cyp3a</title><author>Shimoji, Miyuki ; Itoh, Susumu ; Toide, Kenji ; Nakayama, Kazuo ; Aizawa, Shinichi ; Kamataki, Tetsuya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-89e82c7dcac7145693f6854119ac3ae5ed18d0c6896d94f6821ec6ce1a3adc8f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>7-Alkoxycoumarin O-Dealkylase - metabolism</topic><topic>Animals</topic><topic>Aryl Hydrocarbon Hydroxylases</topic><topic>Cell Division</topic><topic>Cells, Cultured</topic><topic>Cyp3a</topic><topic>Cytochrome P-450 CYP3A</topic><topic>Cytochrome P-450 Enzyme System - genetics</topic><topic>dexamethasone</topic><topic>Dexamethasone - pharmacology</topic><topic>Epidermal Growth Factor - pharmacology</topic><topic>Gene Expression Regulation, Enzymologic - physiology</topic><topic>Hepatocyte Growth Factor - pharmacology</topic><topic>hepatocytes</topic><topic>Insulin - pharmacology</topic><topic>Liver - cytology</topic><topic>Liver - drug effects</topic><topic>Liver - enzymology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Oxidoreductases, N-Demethylating - genetics</topic><topic>p53</topic><topic>primary culture</topic><topic>RNA, Messenger - analysis</topic><topic>Tumor Suppressor Protein p53 - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shimoji, Miyuki</creatorcontrib><creatorcontrib>Itoh, Susumu</creatorcontrib><creatorcontrib>Toide, Kenji</creatorcontrib><creatorcontrib>Nakayama, Kazuo</creatorcontrib><creatorcontrib>Aizawa, Shinichi</creatorcontrib><creatorcontrib>Kamataki, Tetsuya</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biochemistry (Tokyo)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shimoji, Miyuki</au><au>Itoh, Susumu</au><au>Toide, Kenji</au><au>Nakayama, Kazuo</au><au>Aizawa, Shinichi</au><au>Kamataki, Tetsuya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of Primary Hepatocytes from p53-Knockout Mice for Studies of Expression of Cyp3a</atitle><jtitle>Journal of biochemistry (Tokyo)</jtitle><addtitle>J Biochem</addtitle><date>1996-07-01</date><risdate>1996</risdate><volume>120</volume><issue>1</issue><spage>42</spage><epage>48</epage><pages>42-48</pages><issn>0021-924X</issn><abstract>CYP3A rapidly disappears in primary hepatocytes, although the primary cells are suitable for studies of the regulation of CYP3A genes. In the present study, we found that Cyp3a mRNA could be expressed in the primary hepatocytes from p53-knockout mice for at least 2 weeks when the cells were cultured in the presence of dexamethasone. Propoxycoumarin O-depropylase activity, which is known to be mainly catalyzed by CYP3A, was maintained at a level of 50% of the initial activity even after 5 days of culture, and the activity correlated with the expression level of Cyp3a mRNA in the primary hepatocytes from p53-knockout mice. The cells remained morphologically intact during 4 weeks. These results suggest that hepatocytes from p53-knockout mice are a useful tool for studies of the expression of Cyp3a.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>8864842</pmid><doi>10.1093/oxfordjournals.jbchem.a021391</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of biochemistry (Tokyo), 1996-07, Vol.120 (1), p.42-48 |
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| language | eng |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Free Full-Text Journals in Chemistry |
| subjects | 7-Alkoxycoumarin O-Dealkylase - metabolism Animals Aryl Hydrocarbon Hydroxylases Cell Division Cells, Cultured Cyp3a Cytochrome P-450 CYP3A Cytochrome P-450 Enzyme System - genetics dexamethasone Dexamethasone - pharmacology Epidermal Growth Factor - pharmacology Gene Expression Regulation, Enzymologic - physiology Hepatocyte Growth Factor - pharmacology hepatocytes Insulin - pharmacology Liver - cytology Liver - drug effects Liver - enzymology Male Mice Mice, Knockout Oxidoreductases, N-Demethylating - genetics p53 primary culture RNA, Messenger - analysis Tumor Suppressor Protein p53 - physiology |
| title | Application of Primary Hepatocytes from p53-Knockout Mice for Studies of Expression of Cyp3a |
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