Inhibition of penetration of canine zonae pellucidae by homologous spermatoza in vitro using monoclonal antibodies raised against porcine zonae
Ten monoclonal antibodies (MAbs) raised against porcine zonae pellucidae (PZP) were checked for their reactivity against zonae of other species. Two (MAbs A 6 and C 10) out of six MAbs checked reacted with canine zonae as ascertained by indirect immunofluorescence. One of these cross-reactive MAbs (...
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| Veröffentlicht in: | Journal of reproductive immunology 1988-06, Vol.13 (1), p.85-95 |
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| creator | Bamezai, A.K. Mahi-Brown, C.A. Talwar, G.P. |
| description | Ten monoclonal antibodies (MAbs) raised against porcine zonae pellucidae (PZP) were checked for their reactivity against zonae of other species. Two (MAbs A
6 and C
10) out of six MAbs checked reacted with canine zonae as ascertained by indirect immunofluorescence. One of these cross-reactive MAbs (A
6) inhibited the penetration of canine ZP by canine spermatozoa in an in vitro assay by 62.5% and 22% at 10 μg/ml and 1 μg/ml, concentration, respectively. Epitopes recognized by MAbs A
6 and C
10 are present on the 55-kDa glycoprotein of PZP as determined by Western blotting experiments. None of the ten anti-PZP MAbs reacted with zonae of the bonnet monkey, hamster or rabbit. Of the six MAbs checked, none showed cross-reactivity against mouse and cat zonae, and of four checked, none reacted with guinea pig zonae. Only one of the ten showed slight reactivity with human zonae. |
| doi_str_mv | 10.1016/0165-0378(88)90051-4 |
| format | Article |
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6 and C
10) out of six MAbs checked reacted with canine zonae as ascertained by indirect immunofluorescence. One of these cross-reactive MAbs (A
6) inhibited the penetration of canine ZP by canine spermatozoa in an in vitro assay by 62.5% and 22% at 10 μg/ml and 1 μg/ml, concentration, respectively. Epitopes recognized by MAbs A
6 and C
10 are present on the 55-kDa glycoprotein of PZP as determined by Western blotting experiments. None of the ten anti-PZP MAbs reacted with zonae of the bonnet monkey, hamster or rabbit. Of the six MAbs checked, none showed cross-reactivity against mouse and cat zonae, and of four checked, none reacted with guinea pig zonae. Only one of the ten showed slight reactivity with human zonae.</description><identifier>ISSN: 0165-0378</identifier><identifier>EISSN: 1872-7603</identifier><identifier>DOI: 10.1016/0165-0378(88)90051-4</identifier><identifier>PMID: 3418620</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Animals ; Antibodies, Monoclonal ; canine ; Cross Reactions ; Dogs ; Female ; Humans ; In Vitro Techniques ; inhibitory effect ; Male ; monoclonal antibodies ; Ovum - immunology ; porcine ; Species Specificity ; Sperm-Ovum Interactions ; Zona Pellucida - immunology ; zonae pellucidae</subject><ispartof>Journal of reproductive immunology, 1988-06, Vol.13 (1), p.85-95</ispartof><rights>1988</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-c538089184f157888836364fa4dedd58f3825768d0dd43556807465861975b673</citedby><cites>FETCH-LOGICAL-c272t-c538089184f157888836364fa4dedd58f3825768d0dd43556807465861975b673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0165037888900514$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3418620$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bamezai, A.K.</creatorcontrib><creatorcontrib>Mahi-Brown, C.A.</creatorcontrib><creatorcontrib>Talwar, G.P.</creatorcontrib><title>Inhibition of penetration of canine zonae pellucidae by homologous spermatoza in vitro using monoclonal antibodies raised against porcine zonae</title><title>Journal of reproductive immunology</title><addtitle>J Reprod Immunol</addtitle><description>Ten monoclonal antibodies (MAbs) raised against porcine zonae pellucidae (PZP) were checked for their reactivity against zonae of other species. Two (MAbs A
6 and C
10) out of six MAbs checked reacted with canine zonae as ascertained by indirect immunofluorescence. One of these cross-reactive MAbs (A
6) inhibited the penetration of canine ZP by canine spermatozoa in an in vitro assay by 62.5% and 22% at 10 μg/ml and 1 μg/ml, concentration, respectively. Epitopes recognized by MAbs A
6 and C
10 are present on the 55-kDa glycoprotein of PZP as determined by Western blotting experiments. None of the ten anti-PZP MAbs reacted with zonae of the bonnet monkey, hamster or rabbit. Of the six MAbs checked, none showed cross-reactivity against mouse and cat zonae, and of four checked, none reacted with guinea pig zonae. Only one of the ten showed slight reactivity with human zonae.</description><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>canine</subject><subject>Cross Reactions</subject><subject>Dogs</subject><subject>Female</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>inhibitory effect</subject><subject>Male</subject><subject>monoclonal antibodies</subject><subject>Ovum - immunology</subject><subject>porcine</subject><subject>Species Specificity</subject><subject>Sperm-Ovum Interactions</subject><subject>Zona Pellucida - immunology</subject><subject>zonae pellucidae</subject><issn>0165-0378</issn><issn>1872-7603</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UV1rFDEUDaLUtfoPFPIk-jA1mXzdfRGk-FEo9KU-h0yS2UZmkjHJFNo_4V8266772EDIDeeceznnIvSWkgtKqPzUrugIU_AB4OOWEEE7_gxtKKi-U5Kw52hzorxEr0r5RQhVZEvP0BnjFGRPNujPVbwLQ6ghRZxGvPjoazb_v9bEED1-TNH4hk3TaoNr5fCA79KcprRLa8Fl8Xk2NT0aHCK-DzUnvJYQd3hOMdmpqSdsYg1DcsEXnE0o3mGzMyGWipeU7WnKa_RiNFPxb47vOfr57evt5Y_u-ub71eWX6872qq-dFQwIbCnwkQoF7TDJJB8Nd945ASODXigJjjjHmRASiOJSgKRbJQap2Dl6f-i75PR79aXqORTbHJromyetgFPVw57ID0SbUynZj3rJYTb5QVOi93vQ-5D1PmQNoP_tQfMme3fsvw6zdyfRMfiGfz7gvpm8Dz7rYoOP1ruQva3apfD0gL-Mwpkh</recordid><startdate>198806</startdate><enddate>198806</enddate><creator>Bamezai, A.K.</creator><creator>Mahi-Brown, C.A.</creator><creator>Talwar, G.P.</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198806</creationdate><title>Inhibition of penetration of canine zonae pellucidae by homologous spermatoza in vitro using monoclonal antibodies raised against porcine zonae</title><author>Bamezai, A.K. ; Mahi-Brown, C.A. ; Talwar, G.P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-c538089184f157888836364fa4dedd58f3825768d0dd43556807465861975b673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>canine</topic><topic>Cross Reactions</topic><topic>Dogs</topic><topic>Female</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>inhibitory effect</topic><topic>Male</topic><topic>monoclonal antibodies</topic><topic>Ovum - immunology</topic><topic>porcine</topic><topic>Species Specificity</topic><topic>Sperm-Ovum Interactions</topic><topic>Zona Pellucida - immunology</topic><topic>zonae pellucidae</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bamezai, A.K.</creatorcontrib><creatorcontrib>Mahi-Brown, C.A.</creatorcontrib><creatorcontrib>Talwar, G.P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of reproductive immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bamezai, A.K.</au><au>Mahi-Brown, C.A.</au><au>Talwar, G.P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of penetration of canine zonae pellucidae by homologous spermatoza in vitro using monoclonal antibodies raised against porcine zonae</atitle><jtitle>Journal of reproductive immunology</jtitle><addtitle>J Reprod Immunol</addtitle><date>1988-06</date><risdate>1988</risdate><volume>13</volume><issue>1</issue><spage>85</spage><epage>95</epage><pages>85-95</pages><issn>0165-0378</issn><eissn>1872-7603</eissn><abstract>Ten monoclonal antibodies (MAbs) raised against porcine zonae pellucidae (PZP) were checked for their reactivity against zonae of other species. Two (MAbs A
6 and C
10) out of six MAbs checked reacted with canine zonae as ascertained by indirect immunofluorescence. One of these cross-reactive MAbs (A
6) inhibited the penetration of canine ZP by canine spermatozoa in an in vitro assay by 62.5% and 22% at 10 μg/ml and 1 μg/ml, concentration, respectively. Epitopes recognized by MAbs A
6 and C
10 are present on the 55-kDa glycoprotein of PZP as determined by Western blotting experiments. None of the ten anti-PZP MAbs reacted with zonae of the bonnet monkey, hamster or rabbit. Of the six MAbs checked, none showed cross-reactivity against mouse and cat zonae, and of four checked, none reacted with guinea pig zonae. Only one of the ten showed slight reactivity with human zonae.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>3418620</pmid><doi>10.1016/0165-0378(88)90051-4</doi><tpages>11</tpages></addata></record> |
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| ispartof | Journal of reproductive immunology, 1988-06, Vol.13 (1), p.85-95 |
| issn | 0165-0378 1872-7603 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antibodies, Monoclonal canine Cross Reactions Dogs Female Humans In Vitro Techniques inhibitory effect Male monoclonal antibodies Ovum - immunology porcine Species Specificity Sperm-Ovum Interactions Zona Pellucida - immunology zonae pellucidae |
| title | Inhibition of penetration of canine zonae pellucidae by homologous spermatoza in vitro using monoclonal antibodies raised against porcine zonae |
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